Overlap extension PCR cloning: A simple and reliable way to create recombinant plasmids

BioTechniques

Volumn 48, Issue 6, 2010, Pages 463-465

  Bryksin, Anton V ^a   Matsumura, Ichiro ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Overlap extension PCR cloning; Phusion; Recombinant vector; Restriction enzyme ligation independent

Indexed keywords

BETA GALACTOSIDASE; BETA GLUCURONIDASE; DEOXYRIBONUCLEASE IV; DNA POLYMERASE; GREEN FLUORESCENT PROTEIN; RESTRICTION ENDONUCLEASE; RECOMBINANT DNA;

ARTICLE; BACTERIAL CELL; CELL TRANSFORMATION; CHIMERA; ESCHERICHIA COLI; GENE INSERTION; MOLECULAR CLONING; MONITORING; NONHUMAN; OPERON; POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; RECOMBINANT PLASMID; ANIMAL; BACTERIUM TRANSFORMATION; GENETICS; HUMAN; PLASMID; PROCEDURES;

ESCHERICHIA COLI;

ANIMALS; CLONING, MOLECULAR; DNA, RECOMBINANT; ESCHERICHIA COLI; HUMANS; PLASMIDS; POLYMERASE CHAIN REACTION; TRANSFORMATION, BACTERIAL;

EID: 77954129578     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113418     Document Type: Article

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