-
1
-
-
0035712796
-
Directed enzyme evolution
-
Farinas,E.T., Bulter,T. and Arnold,F.H. (2001) Directed enzyme evolution. Curr. Opin. Biotechnol., 12, 545-551.
-
(2001)
Curr. Opin. Biotechnol.
, vol.12
, pp. 545-551
-
-
Farinas, E.T.1
Bulter, T.2
Arnold, F.H.3
-
2
-
-
0036940487
-
Towards the directed evolution of hybrid catalysts
-
Reetz,M.T., Rentzsch,M., Pletsch,A. and Maywald,M. (2002) Towards the directed evolution of hybrid catalysts. Chimia, 56, 721-723.
-
(2002)
Chimia
, vol.56
, pp. 721-723
-
-
Reetz, M.T.1
Rentzsch, M.2
Pletsch, A.3
Maywald, M.4
-
3
-
-
0035903602
-
Investigating and engineering enzymes by genetic selection
-
Taylor,S.V., Kast,P. and Hilvert,D. (2001) Investigating and engineering enzymes by genetic selection. Angew. Chem. Int. Ed. Engl., 40, 3310-3335.
-
(2001)
Angew. Chem. Int. Ed. Engl.
, vol.40
, pp. 3310-3335
-
-
Taylor, S.V.1
Kast, P.2
Hilvert, D.3
-
4
-
-
0037304389
-
Genetic screens and directed evolution for protein solubility
-
Waldo,G.S. (2003) Genetic screens and directed evolution for protein solubility. Curr. Opin. Chem. Biol., 7, 33-38.
-
(2003)
Curr. Opin. Chem. Biol.
, vol.7
, pp. 33-38
-
-
Waldo, G.S.1
-
5
-
-
0036897839
-
Milestones in directed enzyme evolution
-
Tao,H. and Cornish,V.W. (2002) Milestones in directed enzyme evolution. Curr. Opin. Chem. Biol., 6, 858-864.
-
(2002)
Curr. Opin. Chem. Biol.
, vol.6
, pp. 858-864
-
-
Tao, H.1
Cornish, V.W.2
-
6
-
-
0037011406
-
Screening and selection methods for large-scale analysis of protein function
-
Lin,H. and Cornish,V.W. (2002) Screening and selection methods for large-scale analysis of protein function. Angew. Chem. Int. Ed. Engl., 41, 4402-4425.
-
(2002)
Angew. Chem. Int. Ed. Engl.
, vol.41
, pp. 4402-4425
-
-
Lin, H.1
Cornish, V.W.2
-
9
-
-
0028110130
-
DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
-
Stemmer,W.P.C. (1993) DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution. Proc. Natl Acad. Sci. USA, 91, 10747-10751.
-
(1993)
Proc. Natl. Acad. Sci. USA
, vol.91
, pp. 10747-10751
-
-
Stemmer, W.P.C.1
-
10
-
-
0028050350
-
Rapid evolution of a protein in vitro by DNA shuffling
-
Stemmer,W.P.C. (1994) Rapid evolution of a protein in vitro by DNA shuffling. Nature, 370, 389-391.
-
(1994)
Nature
, vol.370
, pp. 389-391
-
-
Stemmer, W.P.C.1
-
11
-
-
0031909113
-
Molecular evolution by staggered extension process (StEP) in vitro recombination
-
Zhao,H., Giver,L., Shao,Z., Affholter,J. and Arnold,F. (1998) Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat. Biotechnol., 16, 258-261.
-
(1998)
Nat. Biotechnol.
, vol.16
, pp. 258-261
-
-
Zhao, H.1
Giver, L.2
Shao, Z.3
Affholter, J.4
Arnold, F.5
-
12
-
-
17444363724
-
A combinatorial approach to hybrid enzymes independent of DNA homology
-
Ostermeier,M., Shim,J.H. and Benkovic,S.J. (1999) A combinatorial approach to hybrid enzymes independent of DNA homology. Nat. Biotechnol., 17, 1205-1209.
-
(1999)
Nat. Biotechnol.
, vol.17
, pp. 1205-1209
-
-
Ostermeier, M.1
Shim, J.H.2
Benkovic, S.J.3
-
13
-
-
0032828603
-
Directed evolution of an esterase: Screening of enzyme libraries based on pH-indicators and a growth assay
-
Bornscheuer,U.T., Altenbuchner,J. and Meyer,H.H. (1999) Directed evolution of an esterase: screening of enzyme libraries based on pH-indicators and a growth assay. Bioorg. Med. Chem., 7, 2169-2173.
-
(1999)
Bioorg. Med. Chem.
, vol.7
, pp. 2169-2173
-
-
Bornscheuer, U.T.1
Altenbuchner, J.2
Meyer, H.H.3
-
14
-
-
0037008967
-
Deracemization of alpha-methylbenzylamine using an enzyme obtained by in vitro evolution
-
Alexeeva,M., Enright,A., Dawson,M.J., Mahmoudian,M. and Turner,N.J. (2002) Deracemization of alpha-methylbenzylamine using an enzyme obtained by in vitro evolution. Angew. Chem. Int. Ed. Engl., 41, 3177-3180.
-
(2002)
Angew. Chem. Int. Ed. Engl.
, vol.41
, pp. 3177-3180
-
-
Alexeeva, M.1
Enright, A.2
Dawson, M.J.3
Mahmoudian, M.4
Turner, N.J.5
-
15
-
-
3042824797
-
Production of randomly mutated plasmid libraries using mutator strains
-
Nguyen,A.W. and Daugherty,P.S. (2003) Production of randomly mutated plasmid libraries using mutator strains. Methods Mol. Biol., 231, 39-44.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 39-44
-
-
Nguyen, A.W.1
Daugherty, P.S.2
-
17
-
-
3042784274
-
Generating mutant libraries using error-prone PCR
-
Cirino,P.C., Mayer,K.M. and Umeno,D. (2003) Generating mutant libraries using error-prone PCR. Methods Mol. Biol., 231, 3-9.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 3-9
-
-
Cirino, P.C.1
Mayer, K.M.2
Umeno, D.3
-
18
-
-
0035846958
-
In vitro evolution of a beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates
-
Matsumura,I. and Ellington,A.D. (2001) In vitro evolution of a beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates. J. Mol. Biol., 305, 331-339.
-
(2001)
J. Mol. Biol.
, vol.305
, pp. 331-339
-
-
Matsumura, I.1
Ellington, A.D.2
-
19
-
-
0036656222
-
Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis
-
Lingen,B., Grotzinger,J., Kolter,D., Kula,M.R. and Pohl,M. (2002) Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis. Protein Eng., 15, 585-593.
-
(2002)
Protein Eng.
, vol.15
, pp. 585-593
-
-
Lingen, B.1
Grotzinger, J.2
Kolter, D.3
Kula, M.R.4
Pohl, M.5
-
20
-
-
0141858719
-
Directed evolution of a bacterial alpha-amylase: Toward enhanced pH-performance and higher specific activity
-
Bessler,C., Schmitt,J., Maurer,K.H. and Schmid,R.D. (2003) Directed evolution of a bacterial alpha-amylase: toward enhanced pH-performance and higher specific activity. Protein Sci., 12, 2141-2149.
-
(2003)
Protein Sci.
, vol.12
, pp. 2141-2149
-
-
Bessler, C.1
Schmitt, J.2
Maurer, K.H.3
Schmid, R.D.4
-
21
-
-
0029869449
-
An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues
-
Zaccolo,M., Williams,D.M., Brown,D.M. and Gheradi,E. (1996) An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues. J. Mol. Biol., 255, 589-603.
-
(1996)
J. Mol. Biol.
, vol.255
, pp. 589-603
-
-
Zaccolo, M.1
Williams, D.M.2
Brown, D.M.3
Gheradi, E.4
-
22
-
-
0033555718
-
The effect of high-frequency random mutagenesis on in vitro protein evolution: A study on TEM-1 β-lactamase
-
Zaccolo,M. and Gherardi,E. (1999) The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 β-lactamase. J. Mol. Biol., 285, 775-783.
-
(1999)
J. Mol. Biol.
, vol.285
, pp. 775-783
-
-
Zaccolo, M.1
Gherardi, E.2
-
23
-
-
0041765676
-
User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries
-
Patrick,W.M., Firth,A.E. and Blackburn,J.M. (2003) User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries. Protein Eng., 16, 451-457.
-
(2003)
Protein Eng.
, vol.16
, pp. 451-457
-
-
Patrick, W.M.1
Firth, A.E.2
Blackburn, J.M.3
-
24
-
-
0041324888
-
A comparison of directed evolution approaches using the beta-glucuronidase model system
-
Rowe,L.A., Geddie,M.L., Alexander,O.B. and Matsumura,I. (2003) A comparison of directed evolution approaches using the beta-glucuronidase model system. J. Mol. Biol., 332, 851-860.
-
(2003)
J. Mol. Biol.
, vol.332
, pp. 851-860
-
-
Rowe, L.A.1
Geddie, M.L.2
Alexander, O.B.3
Matsumura, I.4
-
25
-
-
0033280672
-
Exploring nonnatural evolutionary pathways by saturation mutagenesis: Rapid improvement of protein function
-
Miyazaki,K. and Arnold,F.H. (1999) Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function. J. Mol. Evol., 49, 716-720.
-
(1999)
J. Mol. Evol.
, vol.49
, pp. 716-720
-
-
Miyazaki, K.1
Arnold, F.H.2
-
26
-
-
0036137462
-
Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs
-
Murakami,H., Hohsaka,T. and Sisido,M. (2002) Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs. Nat. Biotechnol., 20, 76-81.
-
(2002)
Nat. Biotechnol.
, vol.20
, pp. 76-81
-
-
Murakami, H.1
Hohsaka, T.2
Sisido, M.3
-
27
-
-
3042821864
-
Random insertion and deletion mutagenesis
-
Murakami,H., Hohsaka,T. and Sisido,M. (2003) Random insertion and deletion mutagenesis. Methods Mol. Biol., 231, 53-64.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 53-64
-
-
Murakami, H.1
Hohsaka, T.2
Sisido, M.3
-
28
-
-
0034548711
-
Pentapeptide scanning mutagenesis: Encouraging old proteins to execute unusual tricks
-
Hayes,F. and Hallet,B. (2000) Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks. Trends Microbiol., 8, 571-577.
-
(2000)
Trends Microbiol.
, vol.8
, pp. 571-577
-
-
Hayes, F.1
Hallet, B.2
-
29
-
-
0037092188
-
Generating segmental mutations in haloalkane dehalogenase: A novel part in the directed evolution toolbox
-
Pikkemaat,M.G. and Janssen,D.B. (2002) Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox. Nucleic Acids Res., 30, e35.
-
(2002)
Nucleic Acids Res.
, vol.30
-
-
Pikkemaat, M.G.1
Janssen, D.B.2
-
30
-
-
0032519301
-
Combinatorial library diversity: Probability assessment of library populations
-
Ward,B. and Juehne,T. (1998) Combinatorial library diversity: probability assessment of library populations. Nucleic Acids Res., 26, 879-886.
-
(1998)
Nucleic Acids Res.
, vol.26
, pp. 879-886
-
-
Ward, B.1
Juehne, T.2
-
31
-
-
0034643921
-
A new randomization assay reveals unexpected elements of sequence bias in model 'randomized' gene libraries: Implications for biopanning
-
Palfrey,D., Picardo,M. and Hine,A.V. (2000) A new randomization assay reveals unexpected elements of sequence bias in model 'randomized' gene libraries: implications for biopanning. Gene, 251, 91-99.
-
(2000)
Gene
, vol.251
, pp. 91-99
-
-
Palfrey, D.1
Picardo, M.2
Hine, A.V.3
-
32
-
-
0028559783
-
Trinucleotide phosphoramidites: Ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis
-
Virnekaes,B., Ge,L., Plueckthun,A. Schneider,K.C., Wellnhofer,G. and Moroney,S.E. (1994) Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. Nucleic Acids Res., 22, 5600-5607.
-
(1994)
Nucleic Acids Res.
, vol.22
, pp. 5600-5607
-
-
Virnekaes, B.1
Ge, L.2
Plueckthun, A.3
Schneider, K.C.4
Wellnhofer, G.5
Moroney, S.E.6
-
33
-
-
1842586731
-
Efficient flexible access to fully protected trinucleotides suitable for DNA synthesis by automated phosphoramidite chemistry
-
Zehl,A., Starke,A., Cech,D., Hartsch,T., Merkl,R. and Fritz,H.J. (1996) Efficient and flexible access to fully protected trinucleotides suitable for DNA synthesis by automated phosphoramidite chemistry. Chem. Commun. Chem. Soc., 2677-2678.
-
(1996)
Chem. Commun. Chem. Soc.
, pp. 2677-2678
-
-
Zehl, A.1
Starke, A.2
Cech, D.3
Hartsch, T.4
Merkl, R.5
Fritz, H.J.6
-
34
-
-
0029810446
-
A convenient approach to the synthesis of trinucleotide phosphoramidites-synthons for the generation of oligonucleotide/peptide libraries
-
Kayushin,A., Korosteleva,M., Miroshnikov,A., Zubov,D., Kosch,W. and Piel,N. (1996) A convenient approach to the synthesis of trinucleotide phosphoramidites-synthons for the generation of oligonucleotide/peptide libraries. Nucleic Acids Res., 24, 3748-3755.
-
(1996)
Nucleic Acids Res.
, vol.24
, pp. 3748-3755
-
-
Kayushin, A.1
Korosteleva, M.2
Miroshnikov, A.3
Zubov, D.4
Kosch, W.5
Piel, N.6
-
35
-
-
0034514421
-
Large-scale solid-phase preparation of 3′-unprotected trinucleotide phosphotriesters-precursors for synthesis of trinucleotide phosphoramidites
-
Kayushin,A., Korosteleva,M. and Miroshnikov,A. (2000) Large-scale solid-phase preparation of 3′-unprotected trinucleotide phosphotriesters-precursors for synthesis of trinucleotide phosphoramidites. Nucleosides Nucleotides Nucleic Acids, 19, 1967-1976.
-
(2000)
Nucleosides Nucleotides Nucleic Acids
, vol.19
, pp. 1967-1976
-
-
Kayushin, A.1
Korosteleva, M.2
Miroshnikov, A.3
-
36
-
-
0035260476
-
Orthogonal combinatorial mutagenesis: A codon-level combinatorial mutagenesis method useful for low multiplicity amino acid-scanning protocols
-
Gaytan,P., Yanez,J., Sanchez,F. and Soberon,X. (2001) Orthogonal combinatorial mutagenesis: a codon-level combinatorial mutagenesis method useful for low multiplicity and amino acid-scanning protocols. Nucleic Acids Res., 29, e9.
-
(2001)
Nucleic Acids Res.
, vol.29
-
-
Gaytan, P.1
Yanez, J.2
Sanchez, F.3
Soberon, X.4
-
37
-
-
0032170417
-
Combination of DMT-mononucleotide and Fmoc-trinucleotide phosphoramidites in oligonucleotide synthesis affords an automatable codon-level mutagenesis method
-
Gaytan,P., Yanez,J., Sanchez,F., Mackie,H. and Soberon,X. (1998) Combination of DMT-mononucleotide and Fmoc-trinucleotide phosphoramidites in oligonucleotide synthesis affords an automatable codon-level mutagenesis method. Chem. Biol., 5, 519-527.
-
(1998)
Chem. Biol.
, vol.5
, pp. 519-527
-
-
Gaytan, P.1
Yanez, J.2
Sanchez, F.3
Mackie, H.4
Soberon, X.5
-
38
-
-
0033592911
-
Patterned library analysis: A method for the quantitative assessment of hypotheses concerning the determinants of protein structure
-
Lahr,S.J., Broadwater,A., Carter,C.W., Jr, Collier,M.L., Hensley,L., Waldner,J.C., Pielak,G.J. and Edgell,M.H. (1999) Patterned library analysis: a method for the quantitative assessment of hypotheses concerning the determinants of protein structure. Proc. Natl Acad. Sci. USA, 96, 14860-14865.
-
(1999)
Proc. Natl. Acad. Sci. USA
, vol.96
, pp. 14860-14865
-
-
Lahr, S.J.1
Broadwater, A.2
Carter Jr., C.W.3
Collier, M.L.4
Hensley, L.5
Waldner, J.C.6
Pielak, G.J.7
Edgell, M.H.8
-
39
-
-
0027082901
-
Antibody engineering by codon-based mutagenesis in a filamentous phage vector system
-
Glaser,S.M., Yelton,D.E. and Huse,W.D. (1992) Antibody engineering by codon-based mutagenesis in a filamentous phage vector system. J. Immunol., 149, 3903-3913.
-
(1992)
J. Immunol.
, vol.149
, pp. 3903-3913
-
-
Glaser, S.M.1
Yelton, D.E.2
Huse, W.D.3
-
40
-
-
0033834791
-
Directed evolution of an enantioselective lipase
-
Liebeton,K., Zonta,A., Schimossek,K., Nardini,M., Lang,D., Dijkstra,B., Reetz,M. and Jaeger,K. (2000) Directed evolution of an enantioselective lipase. Chem. Biol., 7, 709-718.
-
(2000)
Chem. Biol.
, vol.7
, pp. 709-718
-
-
Liebeton, K.1
Zonta, A.2
Schimossek, K.3
Nardini, M.4
Lang, D.5
Dijkstra, B.6
Reetz, M.7
Jaeger, K.8
-
41
-
-
0035464767
-
Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate
-
Sakamoto,T., Joern,J.M., Arisawa,A. and Arnold,F.H. (2001) Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate. Appl. Environ. Microbiol., 67, 3882-3887.
-
(2001)
Appl. Environ. Microbiol.
, vol.67
, pp. 3882-3887
-
-
Sakamoto, T.1
Joern, J.M.2
Arisawa, A.3
Arnold, F.H.4
-
42
-
-
0038814072
-
Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate
-
Horsman,G.P., Liu,A.M., Henke,E., Bornscheuer,U.T. and Kazlauskas,R.J. (2003) Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate. Chemistry, 9, 1933-1939.
-
(2003)
Chemistry
, vol.9
, pp. 1933-1939
-
-
Horsman, G.P.1
Liu, A.M.2
Henke, E.3
Bornscheuer, U.T.4
Kazlauskas, R.J.5
-
43
-
-
0038070162
-
Conversion of cyclodextrin glycosyltransferase into a starch hydrolase by directed evolution: The role of alanine 230 in acceptor subsite +1
-
Leemhuis,H., Rozeboom,H.J., Wilbrink,M., Euverink,G.J., Dijkstra,B.W. and Dijkhuizen,L. (2003) Conversion of cyclodextrin glycosyltransferase into a starch hydrolase by directed evolution: the role of alanine 230 in acceptor subsite +1. Biochemistry, 42, 7518-7526.
-
(2003)
Biochemistry
, vol.42
, pp. 7518-7526
-
-
Leemhuis, H.1
Rozeboom, H.J.2
Wilbrink, M.3
Euverink, G.J.4
Dijkstra, B.W.5
Dijkhuizen, L.6
-
44
-
-
0037401692
-
Directed evolution of N-acetylneuraminic acid aldolase to catalyze enantiomeric aldol reactions
-
Wada,M., Hsu,C.C., Franke,D., Mitchell,M., Heine,A., Wilson,I. and Wong,C.H. (2003) Directed evolution of N-acetylneuraminic acid aldolase to catalyze enantiomeric aldol reactions. Bioorg. Med. Chem., 11, 2091-2098.
-
(2003)
Bioorg. Med. Chem.
, vol.11
, pp. 2091-2098
-
-
Wada, M.1
Hsu, C.C.2
Franke, D.3
Mitchell, M.4
Heine, A.5
Wilson, I.6
Wong, C.H.7
-
45
-
-
0037399074
-
Directed evolution of O(6)-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo
-
Juillerat,A., Gronemeyer,T., Keppler,A., Gendreizig,S., Pick,H., Vogel,H. and Johnsson,K. (2003) Directed evolution of O(6)-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo. Chem. Biol., 10, 313-317.
-
(2003)
Chem. Biol.
, vol.10
, pp. 313-317
-
-
Juillerat, A.1
Gronemeyer, T.2
Keppler, A.3
Gendreizig, S.4
Pick, H.5
Vogel, H.6
Johnsson, K.7
-
46
-
-
0036379384
-
Directed evolution of a glutaryl acylase into an adipyl acylase
-
Sio,C.F., Riemens,A.M., van der Laan,J.M., Verhaert,R.M. and Quax,W.J. (2002) Directed evolution of a glutaryl acylase into an adipyl acylase. Eur. J. Biochem., 269, 4495-4504.
-
(2002)
Eur. J. Biochem.
, vol.269
, pp. 4495-4504
-
-
Sio, C.F.1
Riemens, A.M.2
van der Laan, J.M.3
Verhaert, R.M.4
Quax, W.J.5
-
47
-
-
43949114931
-
Saturation mutagenesis
-
Georgescu,R., Bandara,G. and Sun,L. (2003) Saturation mutagenesis. Methods Mol. Biol., 231, 75-83.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 75-83
-
-
Georgescu, R.1
Bandara, G.2
Sun, L.3
-
48
-
-
1842842876
-
Creating random mutagenesis libraries using megaprimer PCR of whole plasmid
-
1033-1034
-
Miyazaki,K. and Takenouchi,M. (2002) Creating random mutagenesis libraries using megaprimer PCR of whole plasmid. Biotechniques, 33, 1033-1034, 1036-1038.
-
(2002)
Biotechniques
, vol.33
, pp. 1036-1038
-
-
Miyazaki, K.1
Takenouchi, M.2
-
49
-
-
3042784273
-
Creating random mutagenesis libraries by megaprimer PCR of whole plasmid (MEGAWHOP)
-
Miyazaki,K. (2003) Creating random mutagenesis libraries by megaprimer PCR of whole plasmid (MEGAWHOP). Methods Mol. Biol., 231, 23-28.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 23-28
-
-
Miyazaki, K.1
-
50
-
-
0037415021
-
Assembly of designed oligonucleotides as an efficient method for gene recombination: A new tool in directed evolution
-
Zha,D., Eipper,A. and Reetz,M.T. (2003) Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution. Chembiochemistry, 4, 34-39.
-
(2003)
Chembiochemistry
, vol.4
, pp. 34-39
-
-
Zha, D.1
Eipper, A.2
Reetz, M.T.3
-
51
-
-
0036901312
-
Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently
-
Ness,J.E., Kim,S., Gottman,A., Pak,R., Krebber,A., Borchert,T.V., Govindarajan,S., Mundorff,E.C. and Minshull,J. (2002) Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently. Nat. Biotechnol., 20, 1251-1255.
-
(2002)
Nat. Biotechnol.
, vol.20
, pp. 1251-1255
-
-
Ness, J.E.1
Kim, S.2
Gottman, A.3
Pak, R.4
Krebber, A.5
Borchert, T.V.6
Govindarajan, S.7
Mundorff, E.C.8
Minshull, J.9
-
52
-
-
0036841628
-
Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit
-
1162 1158-1160
-
Hogrefe,H.H., Cline,J., Youngblood,G.L. and Allen,R.M. (2002) Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit. Biotechniques, 33, 1158-1160, 1162, 1164-1165.
-
(2002)
Biotechniques
, vol.33
, pp. 1164-1165
-
-
Hogrefe, H.H.1
Cline, J.2
Youngblood, G.L.3
Allen, R.M.4
-
53
-
-
0042121557
-
Removing the redundancy from randomised gene libraries
-
Hughes,M.D., Nagel,D.A., Santos,A.F., Sutherland,A.J. and Hine,A.V. (2003) Removing the redundancy from randomised gene libraries. J. Mol. Biol., 331, 973-979.
-
(2003)
J. Mol. Biol.
, vol.331
, pp. 973-979
-
-
Hughes, M.D.1
Nagel, D.A.2
Santos, A.F.3
Sutherland, A.J.4
Hine, A.V.5
-
54
-
-
3042826371
-
DNA shuffling
-
Joern,J.M. (2003) DNA shuffling. Methods Mol. Biol., 231, 85-89.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 85-89
-
-
Joern, J.M.1
-
55
-
-
1542323289
-
Staggered extension process (StEP) in vitro recombination
-
Aguinaldo,A.M. and Arnold,F.H. (2003) Staggered extension process (StEP) in vitro recombination. Methods Mol. Biol., 231, 105-110.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 105-110
-
-
Aguinaldo, A.M.1
Arnold, F.H.2
-
56
-
-
0035067250
-
DNA shuffling method for generating highly recombined genes evolved enzymes
-
Coco,W.M., Levinson,W.E., Crist,M.J., Hektor,H.J., Darzins,A., Pienkos,P.T., Squires,C.H. and Monticello,D.J. (2001) DNA shuffling method for generating highly recombined genes and evolved enzymes. Nat. Biotechnol., 19, 354-359.
-
(2001)
Nat. Biotechnol.
, vol.19
, pp. 354-359
-
-
Coco, W.M.1
Levinson, W.E.2
Crist, M.J.3
Hektor, H.J.4
Darzins, A.5
Pienkos, P.T.6
Squires, C.H.7
Monticello, D.J.8
-
57
-
-
1542353447
-
RACHITT: Gene family shuffling by Random Chimeragenesis on Transient Templates
-
Coco,W.M. (2003) RACHITT: Gene family shuffling by Random Chimeragenesis on Transient Templates. Methods Mol. Biol., 231, 111-127.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 111-127
-
-
Coco, W.M.1
-
58
-
-
0035866186
-
Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides
-
Lutz,S., Ostermeier,M. and Benkovic,S.J. (2001) Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides. Nucleic Acids Res., 29, e16.
-
(2001)
Nucleic Acids Res.
, vol.29
-
-
Lutz, S.1
Ostermeier, M.2
Benkovic, S.J.3
-
59
-
-
3042739160
-
The creation of ITCHY hybrid protein libraries
-
Ostermeier,M. and Lutz,S. (2003) The creation of ITCHY hybrid protein libraries. Methods Mol. Biol., 231, 129-141.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 129-141
-
-
Ostermeier, M.1
Lutz, S.2
-
60
-
-
3042702522
-
Preparation of SCRATCHY hybrid protein libraries: Size- In-frame selection of nucleic acid sequences
-
Lutz,S. and Ostermeier,M. (2003) Preparation of SCRATCHY hybrid protein libraries: size- and in-frame selection of nucleic acid sequences. Methods Mol. Biol., 231, 143-151.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 143-151
-
-
Lutz, S.1
Ostermeier, M.2
-
61
-
-
0038606300
-
Forced evolution of a herbicide detoxifying glutathione transferase
-
Dixon,D.P., McEwen,A.G., Lapthorn,A.J. and Edwards,R. (2003) Forced evolution of a herbicide detoxifying glutathione transferase. J. Biol. Chem., 278, 23930-23935.
-
(2003)
J. Biol. Chem.
, vol.278
, pp. 23930-23935
-
-
Dixon, D.P.1
McEwen, A.G.2
Lapthorn, A.J.3
Edwards, R.4
-
62
-
-
0038521054
-
Significantly enhanced stability of glucose dehydrogenase by directed evolution
-
Baik,S.H., Ide,T., Yoshida,H., Kagami,O. and Harayama,S. (2003) Significantly enhanced stability of glucose dehydrogenase by directed evolution. Appl. Microbiol. Biotechnol., 61, 329-335.
-
(2003)
Appl. Microbiol. Biotechnol.
, vol.61
, pp. 329-335
-
-
Baik, S.H.1
Ide, T.2
Yoshida, H.3
Kagami, O.4
Harayama, S.5
-
63
-
-
0037114242
-
Random DNA fragmentation with endonuclease V: Application to DNA shuffling
-
Miyazaki,K. (2002) Random DNA fragmentation with endonuclease V: application to DNA shuffling. Nucleic Acids Res., 30, e139.
-
(2002)
Nucleic Acids Res.
, vol.30
-
-
Miyazaki, K.1
-
64
-
-
0036842594
-
Laboratory evolution of a soluble self-sufficient highly active alkane hydroxylase
-
Glieder,A., Farinas,E.T. and Arnold,F.H. (2002) Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase. Nat. Biotechnol., 20, 1135-1139.
-
(2002)
Nat. Biotechnol.
, vol.20
, pp. 1135-1139
-
-
Glieder, A.1
Farinas, E.T.2
Arnold, F.H.3
-
65
-
-
0035183586
-
Expression stabilization of galactose oxidase in Escherichia coli by directed evolution
-
Sun,L., Petrounia,I.P., Yagasaki,M., Bandara,G. and Arnold,F.H. (2001) Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng., 14, 699-704.
-
(2001)
Protein Eng.
, vol.14
, pp. 699-704
-
-
Sun, L.1
Petrounia, I.P.2
Yagasaki, M.3
Bandara, G.4
Arnold, F.H.5
-
66
-
-
0035853135
-
Predicting crossover generation in DNA shuffling
-
Moore,G.L., Maranas,C.D., Lutz,S. and Benkovic,S.J. (2001) Predicting crossover generation in DNA shuffling. Proc. Natl Acad. Sci. USA, 98, 3226-3231.
-
(2001)
Proc. Natl. Acad. Sci. USA
, vol.98
, pp. 3226-3231
-
-
Moore, G.L.1
Maranas, C.D.2
Lutz, S.3
Benkovic, S.J.4
-
67
-
-
0036606766
-
eCodonOpt: A systematic computational framework for optimizing codon usage in directed evolution experiments
-
Moore,G.L. and Maranas,C.D. (2002) eCodonOpt: a systematic computational framework for optimizing codon usage in directed evolution experiments. Nucleic Acids Res., 30, 2407-2416.
-
(2002)
Nucleic Acids Res.
, vol.30
, pp. 2407-2416
-
-
Moore, G.L.1
Maranas, C.D.2
-
68
-
-
0036307433
-
Analysis of shuffled gene libraries
-
Joern,J.M., Meinhold,P. and Arnold,F.H. (2002) Analysis of shuffled gene libraries. J. Mol. Biol., 316, 643-656.
-
(2002)
J. Mol. Biol.
, vol.316
, pp. 643-656
-
-
Joern, J.M.1
Meinhold, P.2
Arnold, F.H.3
-
69
-
-
0032814117
-
Novel family shuffling methods for the in vitro evolution of enzymes
-
Kikuchi,M., Ohnishi,K. and Harayama,S. (1999) Novel family shuffling methods for the in vitro evolution of enzymes. Gene, 236, 159-167.
-
(1999)
Gene
, vol.236
, pp. 159-167
-
-
Kikuchi, M.1
Ohnishi, K.2
Harayama, S.3
-
70
-
-
0033964975
-
An effective family shuffling method using single-stranded DNA
-
Kikuchi,M., Ohnishi,K. and Harayama,S. (2000) An effective family shuffling method using single-stranded DNA. Gene, 243, 133-137.
-
(2000)
Gene
, vol.243
, pp. 133-137
-
-
Kikuchi, M.1
Ohnishi, K.2
Harayama, S.3
-
71
-
-
1542263494
-
Family shuffling with single-stranded DNA
-
Zha,W., Zhu,T. and Zhao,H. (2003) Family shuffling with single-stranded DNA. Methods Mol. Biol., 231, 91-97.
-
(2003)
Methods Mol. Biol.
, vol.231
, pp. 91-97
-
-
Zha, W.1
Zhu, T.2
Zhao, H.3
-
72
-
-
0035854021
-
Degenerate oligonucleotide gene shuffling (DOGS): A method for enhancing the frequency of recombination with family shuffling
-
Gibbs,M.D., Nevalainen,K.M. and Bergquist,P.L. (2001) Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling. Gene, 271, 13-20.
-
(2001)
Gene
, vol.271
, pp. 13-20
-
-
Gibbs, M.D.1
Nevalainen, K.M.2
Bergquist, P.L.3
-
73
-
-
0036933287
-
A universal vector-based system for nucleic acid reading-frame selection
-
Lutz,S., Fast,W. and Benkovic,S.J. (2002) A universal, vector-based system for nucleic acid reading-frame selection. Protein Eng., 15, 1025-1030.
-
(2002)
Protein Eng.
, vol.15
, pp. 1025-1030
-
-
Lutz, S.1
Fast, W.2
Benkovic, S.J.3
-
74
-
-
0035949702
-
Creating multiple-crossover DNA libraries independent of sequence identity
-
Lutz,S., Ostermeier,M., Moore,G.L., Maranas,C.D. and Benkovic,S.J. (2001) Creating multiple-crossover DNA libraries independent of sequence identity. Proc. Natl Acad. Sci. USA, 98, 11248-11253.
-
(2001)
Proc. Natl. Acad. Sci. USA
, vol.98
, pp. 11248-11253
-
-
Lutz, S.1
Ostermeier, M.2
Moore, G.L.3
Maranas, C.D.4
Benkovic, S.J.5
-
75
-
-
0642334596
-
Enhanced crossover SCRATCHY: Construction high-throughput screening of a combinatorial library containing multiple non-homologous crossovers
-
Kawarasaki,Y., Griswold,K.E., Stevenson,J.D., Selzer,T., Benkovic,S.J., Iverson,B.L. and Georgiou,G. (2003) Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers. Nucleic Acids Res., 31, e126.
-
(2003)
Nucleic Acids Res.
, vol.31
-
-
Kawarasaki, Y.1
Griswold, K.E.2
Stevenson, J.D.3
Selzer, T.4
Benkovic, S.J.5
Iverson, B.L.6
Georgiou, G.7
-
76
-
-
0036960601
-
Structure-based combinatorial protein engineering (SCOPE)
-
O'Maille,P.E., Bakhtina,M. and Tsai,M.D. (2002) Structure-based combinatorial protein engineering (SCOPE). J. Mol. Biol., 321, 677-691.
-
(2002)
J. Mol. Biol.
, vol.321
, pp. 677-691
-
-
O'Maille, P.E.1
Bakhtina, M.2
Tsai, M.D.3
-
77
-
-
0038799745
-
General method for sequence-independent site-directed chimeragenesis
-
Hiraga,K. and Arnold,F.H. (2003) General method for sequence-independent site-directed chimeragenesis. J. Mol. Biol., 330, 287-296.
-
(2003)
J. Mol. Biol.
, vol.330
, pp. 287-296
-
-
Hiraga, K.1
Arnold, F.H.2
-
78
-
-
0036289574
-
Protein building blocks preserved by recombination
-
Voigt,C.A., Martinez,C., Wang,Z.G., Mayo,S.L. and Arnold,F.H. (2002) Protein building blocks preserved by recombination. Nat. Struct. Biol., 9, 553-558.
-
(2002)
Nat. Struct. Biol.
, vol.9
, pp. 553-558
-
-
Voigt, C.A.1
Martinez, C.2
Wang, Z.G.3
Mayo, S.L.4
Arnold, F.H.5
-
79
-
-
0038731100
-
Library analysis of SCHEMA-guided protein recombination
-
Meyer,M.M., Silberg,J.J., Voigt,C.A., Endelman,J.B., Mayo,S.L., Wang,Z.G. and Amold,F.H. (2003) Library analysis of SCHEMA-guided protein recombination. Protein Sci., 12, 1686-1693.
-
(2003)
Protein Sci.
, vol.12
, pp. 1686-1693
-
-
Meyer, M.M.1
Silberg, J.J.2
Voigt, C.A.3
Endelman, J.B.4
Mayo, S.L.5
Wang, Z.G.6
Amold, F.H.7
-
80
-
-
0031587291
-
Strategies for the in vitro evolution of protein function: Enzyme evolution by random recombination of improved sequences
-
Moore,J.C., Jin,H.-M., Kuchner,O. and Arnold,F.H. (1997) Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences. J. Mol. Biol., 272, 336-347.
-
(1997)
J. Mol. Biol.
, vol.272
, pp. 336-347
-
-
Moore, J.C.1
Jin, H.-M.2
Kuchner, O.3
Arnold, F.H.4
|