Construction of "small-intelligent" focused mutagenesis libraries using well-designed combinatorial degenerate primers

BioTechniques

Volumn 52, Issue 3, 2012, Pages 149-158

  Tang, Lixia ^a   Gao, Hui ^a   Zhu, Xuechen ^a   Wang, Xiong ^a   Zhou, Ming ^a   Jiang, Rongxiang ^a  

^a UNIVERSITY OF ELECTRONIC SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

Amino acid bias; Codon redundancy; Degenerate codon design; Library construction; Randomization

Indexed keywords

AMINO ACID;

ARTICLE; ESCHERICHIA COLI; GENE LIBRARY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; STOP CODON;

ARTIFICIAL INTELLIGENCE; CODON; COMPUTATIONAL BIOLOGY; DNA PRIMERS; ESCHERICHIA COLI; GENE LIBRARY; GENES, BACTERIAL; HYDROLASES; MUTAGENESIS, SITE-DIRECTED; SOFTWARE;

ESCHERICHIA COLI;

EID: 84863280525     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113820     Document Type: Article

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