Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries

Nature Protocols

Volumn 6, Issue 2, 2011, Pages 242-251

  Quan, Jiayuan ^a,b   Tian, Jingdong ^a,b  

^a Duke University   (United States)

^b DUKE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE;

ARTICLE; CIRCULAR POLYMERASE EXTENSION CLONING; COMBINATORIAL LIBRARY; DNA FRAGMENTATION; DNA LIBRARY; DNA SEQUENCE; GENE EXPRESSION; HOMOLOGOUS RECOMBINATION; MACROMOLECULE; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

CLONING, MOLECULAR; DNA PRIMERS; ESCHERICHIA COLI; GENE LIBRARY; GENETIC VECTORS; LAC OPERON; PLASMIDS; TRANSFORMATION, GENETIC;

EID: 79551667941     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.181     Document Type: Article

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