"Potential health benefits of lunasin: A multifaceted soy-derived bioactive peptide"

Journal of Food Science

Volumn 80, Issue 3, 2015, Pages C485-C494

  Lule, Vaibhao Kisanrao ^a   Garg, Sheenam ^a   Pophaly, Sarang Dilip ^b   Hitesh, ^a   Tomar, Sudhir Kumar ^a  

^a NATIONAL DAIRY RESEARCH INSTITUTE   (India)

^b Indira Gandhi Krishi Vishwavidyalaya   (India)

Author keywords

Anti inflammatory; Anticancerous; Antioxidative; LDL cholesterol; Lunasin

Indexed keywords

GLYCINE MAX;

GM2S-1 PROTEIN, GLYCINE MAX; PEPTIDE; SOYBEAN PROTEIN;

CHEMISTRY; DIET SUPPLEMENTATION; HEALTH; HUMAN; PHYTOTHERAPY; PLANT SEED; SOYBEAN;

DIETARY SUPPLEMENTS; HEALTH; HUMANS; PEPTIDES; PHYTOTHERAPY; SEEDS; SOYBEAN PROTEINS; SOYBEANS;

EID: 84925343564     PISSN: 00221147     EISSN: 17503841     Source Type: Journal    
DOI: 10.1111/1750-3841.12786     Document Type: Article

References (93)

1. Adams MR, Golden DL, Franke AA, Potter SM, Smith HS, Anthony MS. 2004. Dietary soy β-conglycinin (7S globulin) inhibits atherosclerosis in mice. The J Nutr 134:511-6.
2. Adlercreutz H, Mazur W. 1997. Phyto-oestrogens and Western diseases. Ann Med 29:95-120.
3. Allavena P, Garlanda C, Borrello MG, Sica A, Mantovani A. 2008. Pathways connecting inflammation and cancer. Curr Opin Genet Dev 18:3-10.
4. Anderson JW. Johnstone BM, Cook-Newell ME. 1995. Meta-analysis of the effects of soy protein intake on serum lipids. New Engl J Med 333:276-82.
5. Cam A, De Mejia EG. 2012. RGD-peptide lunasin inhibits Akt-mediated NF-κB activation in human macrophages through interaction with the αVβ3 integrin. Mol Nutr Food Res 56:1569-81.
6. Cavazos A, Morales E, Dia VP, Mejia D, Gonzalez E. 2012. Analysis of lunasin in commercial and pilot plant produced soybean products and an improved method of lunasin purification. J Food Sci 77:C539-45.
7. Chang HC, Lewis D, Tung CY, Han L, Henriquez SM, Voiles L, Lupov IP, Pelloso D, Sinn AL, Pollok KE, De Lumen BO, Li F, Blum JS, Srivastava S, Robertson MJ. 2014. Soypeptide lunasin in cytokine immunotherapy for lymphoma. Cancer Immunol Immunother 63:283-95.
8. Cohen LA, Zhao Z, Pittman BSJA, Scimeca JA. 2000. Effect of intact and isoflavone-depleted soy protein on NMU-induced rat mammary tumorigenesis. Carcinogenesis 21:929-35.
9. Dai Q, Franke AA, Jin F, Shu XO, Hebert JR, Custer LJ, Cheng J, Gao YT, Zheng W. 2002. Urinary excretion of phytoestrogens and risk of breast cancer among Chinese women in Shanghai. Cancer Epidem Biomar 11:815-21.
10. Davis KR, Barnett B, Seber L. 2014. U.S. Patent Nr. 8, 759, 613. Washington, DC: U.S. Patent and Trademark Office.
11. Dia VP, De Mejia EG. 2011a. Lunasin induces apoptosis and modifies the expression of genes associated with extracellular matrix and cell adhesion in human metastatic colon cancer cells. Mol Nutr Food Res 55:623-34.
12. Dia VP, De Mejia EG. 2011b. Lunasin potentiates the effect of oxaliplatin preventing outgrowth of colon cancer metastasis, binds to alpha5beta1 integrin and suppresses FAK/ERK/NF-kappaB signaling. Cancer Lett 313:167-80.
13. Dia VP, De Mejia EG. 2011c. Differential gene expression of RAW 264.7 macrophages in response to the RGD peptide lunasin with and without lipopolysaccharide stimulation. Peptides 32:1979-88.
14. Dia VP, Mejia EGD. 2010. Lunasin promotes apoptosis in human colon cancer cells by mitochondrial pathway activation and induction of nuclear clusterin expression. Cancer Lett 295:44-53.
15. Dia VP, Berhow MA, Gonzalez De Mejia E. 2008. Bowman-Birk inhibitor and genistein among soy compounds that synergistically inhibit nitric oxide and prostaglandin E2 pathways in lipopolysaccharide-induced macrophages. J Agr Food Chem 56:11707-17.
16. Dia VP, Torres S, De Lumen BO, Erdman Jr JW, Gonzalez De Mejia E. 2009. Presence of lunasin in plasma of men after soy protein consumption. J Agr Food Chem 57(4):1260-6.
17. Dinelli G, Bregola V, Bosi S, Fiori J, Gotti R, Simonetti E, Trozzi C, Leoncini E, Prata C, Massaccesi L, Malaguti M, Quinn R, Hrelia S. 2014. Lunasin in wheat: a chemical and molecular study on its presence or absence. Food Chem 151:520-5.
18. Fernandez-Tome S, Ramos S, Cordero-Herrera I, Recio I, Goya L, Hernandez-Ledesma B. 2014. In vitro chemo-protective effect of bioactive peptide lunasin against oxidative stress in human HepG2 cells. Food Res Int 62:793-800.
19. th (1999), United States Dept. of Health and Human Services. Available from: http://www.fda.gov/bbs/topics/ANSWERS/ANS00980.Html.
20. Fournier DB, Erdman JW, Gordon GB. 1998. Soy, its components, and cancer prevention: a review of the in vitro, animal, and human data. Cancer Epidem Biomar 7:1055-65.
21. Galvez AF. 2010. Methods for using soy peptides to inhibit H3 acetylation, reduce expression of HMG CoA reductase, and increase LDL receptor and Sp1 expression in a mammal. U.S. Patent 7731995.
22. Galvez AF. 2012. Abstract: identification of lunasin as the active component in soy protein responsible for reducing LDL cholesterol and risk of cardiovascular disease. Circ Res 126:A10693.
23. Galvez AF. 2013. Products and methods using soy peptides to lower total and LDL cholesterol levels. U.S. Patent 8598111.
24. Galvez AF, Benito O. 1999. A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nature Biotech 17:495-500.
25. Galvez AF, Revilleza MJ, De Lumen BO. 1997. A novel methionine-rich protein from soybean cotyledon: cloning and characterization of cDNA (Accession Nr. AF005030) (PGR #97-103). Plant Physiol 114:1567.
26. Galvez AF, Chen N, Macasieb J, Ben O. 2001. Chemopreventive property of a soybean peptide (lunasin) that binds to deacetylated histones and inhibits acetylation. Cancer Res 61:7473-8.
27. Galvez AF, Chunjiang F, Porter J, Kerley M, Presented abstract: epigenetic regulation of HMG-COA reductase and LDL receptor expression by the soy peptide lunasin provides mechanism for lowering cholesterol. Available from: http://www.lunasin.com/ScientificResearch/LunasinResearchAbstracts.aspx#AnchorFortyOne. Accessed on 2014, May 10.
28. Garcia-Nebot MJ, Recio I, Hernandez-Ledesma B. 2014. Antioxidant activity and protective effects of peptide lunasin against oxidative stress in intestinal Caco-2 cells. Food Chem Toxicol 65:155-61.
29. Genovese MI, Lajolo FM. 2001. Isoflavones in soy-based foods consumed in Brazil: levels, distribution, and estimated intake. J Agr Food Chem 50:5987-93.
30. Gobbetti M, De Angelis M, Corsetti A, Di Cagno R. 2005. Biochemistry and physiology of sourdough lactic acid bacteria. Trends Food Sci Technol 16:57-9.
31. Gonzalez De Mejia E, Dia VP. 2009. Lunasin and lunasin-like peptides inhibit inflammation through suppression of NF-kappaB pathway in the macrophage. Peptides 30:2388-98.
32. Gonzalez De Mejia E, Dia VP. 2010. Chemistry and biological properties of soybean peptides and proteins. In ACS symposium series (Oxford Univ. Press) 1059:133-54.
33. Gonzalez De Mejia E, Vasconez M, De Lumen BO, Nelson R. 2004. Lunasin concentration in different soybean genotypes, commercial soy protein, and isoflavone products. J Agr Food Chem 52:5882-7.
34. Gonzalez De Mejia E, De Lumen BO. 2006. Soybean bioactive peptides: a new horizon in preventing chronic diseases. Sex Reprod Menopause 4:91-5.
35. Gonzalez De Mejia E, Wang W, Dia VP. 2010. Lunasin with an arginine-glycine-aspartic acid motif, causes apoptosis to L1210 leukemia cells by activation of caspase-3. Mol Nutr Food Res 54:406-14.
36. Goodman MT, Wilkens LR, Hankin JH, Lyu LC, Wu AH, Kolonel LN. 1997. Association of soy and fiber consumption with the risk of endometrial cancer. Am J Epidemiol 146:294-306.
37. Greaves KA, Parks JS, Williams JK, Wagner JD. 1999. Intact dietary soy protein, but not adding an isoflavone-rich soy extract to casein, improves plasma lipids in ovariectomized cynomolgus monkeys. J Nutr 129:1585-92.
38. Hernandez-Ledesma B, Hsieh CC, De Lumen BO. 2009a. Lunasin, a novel seed peptide for cancer prevention. Peptides 30:426-30.
39. Hernandez-Ledesma B, Hsieh CC, De Lumen BO. 2009b. Antioxidant and anti-inflammatory properties of cancer preventive peptide lunasin in RAW 264.7 macrophages. Biochem Bioph Res Co 390:803-8.
40. Hernandez-Ledesma B, Hsieh CC, De Lumen BO. 2011. Relationship between lunasin's sequence and its inhibitory activity of histones H3 and H4 acetylation. Mol Nutr Food Res 55:989-98.
41. Hernandez-Ledesma B, Ben O, Hsieh CC. 2013. 1997-2012: Fifteen Years of Research on Peptide Lunasin, In: Bioactive Food Peptides in Health and Disease, Dr. Blanca Hernandez-Ledesma (Ed.), P.p 3-22, DOI: 10.5772/52368.
42. Hsieh EA, Chai CM, De Lumen BO, Neese RA, Hellerstein MK. 2004. Dynamics of keratinocytes in vivo using 2H2O labeling: a sensitive marker of epidermal proliferation state. J Invest Dermatol 123:530-6.
43. Hsieh CC, Hernandez-Ledesma B, De Lumen BO. 2010a. Lunasin, a novel seed peptide, sensitizes human breast cancer MDA-MB-231 cells to aspirin-arrested cell cycle and induced apoptosis. Chem Biol Interact 186:127-34.
44. Hsieh CC, Hernandez-Ledesma B, Jeong HJ, Park JH, Ben O. 2010b. Complementary roles in cancer prevention: protease inhibitor makes the cancer preventive peptide lunasin bioavailable. PLoS One 5:e8890.
45. Jacobsen BK, Knutsen SF, Fraser GE. 1998. Does high soy milk intake reduce prostate cancer incidence? The Adventist Health Study (United States). Cancer Cause Control 9:553-7.
46. Jeong HJ, Lam Y, De Lumen BO. 2002. Barley lunasin suppresses ras-induced colony formation and inhibits core histone acetylation in mammalian cells. J Agr Food Chem 50:5903-8.
47. Jeong HJ, Park JH, Lam Y, De Lumen BO. 2003. Characterization of lunasin isolated from soybean. J Agr Food Chem 51:7901-6.
48. Jeong HJ, Jeong JB, Kim DS, Park JH, Lee JB, Kweon DH, Chung GY, Seo EW, De Lumen BO. 2007a. The cancer preventive peptide lunasin from wheat inhibits core histone acetylation. Cancer Lett 255:42-8.
49. Jeong JB, Jeong HJ, Park JH, Lee SH, Lee JR, Lee HK, Chung GY, Choi JD, De Lumen BO. 2007b. Cancer-preventive peptide lunasin from Solanum nigrum L. inhibits acetylation of core histones H3 and H4 and phosphorylation of retinoblastoma protein (Rb). J Agr Food Chem 55:10707-13.
50. Jeong HJ, Jeong JB, Kim DS, De Lumen BO. 2007c. Inhibition of core histone acetylation by the cancer preventive peptide lunasin. J Agr Food Chem 55:632-7.
51. Jeong HJ, Lee JR, Jeong JB, Park JH, Cheong YK, de Lumen BO. 2009. The cancer preventive seed peptide lunasin from rye is bioavailable and bioactive. Nutr Cancer 61:680-6.
52. Jeong JB, De Lumen BO, Jeong HJ. 2010. Lunasin peptide purified from Solanum nigrum L. protects DNA from oxidative damage by suppressing the generation of hydroxyl radical via blocking fenton reaction. Cancer Lett 293:58-64.
53. Jones G, Srivastava A. 2014. Understanding Lunasin's biology and potential as a cancer therapeutic by utilizing Drosophila genetics. Exp Biol Med 239:519-28.
54. Kapoor S. 2014. Lunasin: attenuating effects on tumour growth in systemic malignancies. Food Chem 150:174.
55. Kennedy AR. 1998. The Bowman-Birk inhibitor from soybeans as an anticarcinogenic agent. Am J Clin Nutr 68:1406S-12S.
56. Khan N, Afaq F, Mukhtar H. 2008. Cancer chemoprevention through dietary antioxidants: progress and promise. Antioxid Redox Signal 10:475-510.
57. Kulling SE, Honig DM, Metzler M. 2001. Oxidative metabolism of the soy isoflavones daidzein and genistein in humans in vitro and in vivo. J Agr Food Chem 49:3024-33.
58. Kuper H, Adami HO, Trichopoulos D. 2000. Infections as a major preventable cause of human cancer. J Iintern Med 248:171-83.
59. Kyle S, James, KA, McPherson MJ. 2012. Recombinant production of the therapeutic peptide lunasin. Microb Cell Fact 11:1-8.
60. Lam Y, Galvez A, De Lumen BO. 2003. Lunasin™ suppresses E1A-mediated transformation of mammalian cells but does not inhibit growth of immortalized and established cancer cell lines. Nutr Cancer 47:88-94.
61. Lee MM, Gomez SL, Chang JS, Wey M, Wang RT, Hsing AW. 2003. Soy and isoflavone consumption in relation to prostate cancer risk in China. Cancer Epidem Biomar 12:665-8.
62. Liu CF, Pan TM. 2010. Recombinant expression of bioactive peptide lunasin in Escherichia coli. Appl Microbiol Biotechnol 88:177-86.
63. Losso JN. 2008. The biochemical and functional food properties of the Bowman-Birk inhibitor. Crit Rev Food Sci 48:94-118.
64. Lumen BO. 2005. Nutr Rev 63:16-21.
65. Lunasin. Available from: http://www.lunasin.com. Accessed on 2014 May 10.
66. McCue P, Shetty K. 2004. Health benefits of soy isoflavonoids and strategies for enhancement: a review. Crit Rev Food Sci 44:361-7.
67. Messina M, Flickinger B. 2002. Hypothesized anticancer effects of soy: evidence points to isoflavones as the primary anticarcinogens. Pharm Biol 40:6-23.
68. Mitchell RA, Lovegrove A, Shewry PR. 2013. Lunasin in cereal seeds: what is the origin? J Cereal Sci 57:267-9.
69. Nakurte I, Kirhnere I, Namniece J, Saleniece K, Krigere L, Mekss P, Vicupe Z, Bleidere M, Legzdina L, Muceniece R. 2013. Detection of the lunasin peptide in oats (Avena sativa L). J Cereal Sci 57:319-24.
70. Odani S, Koide T, Ono T. 1987. Amino acid sequence of a soybean (Glycine max) seed polypeptide having a poly (L-aspartic acid) structure. J Biol Chem 262:10502-5.
71. Omoni AO, Aluko RE. 2005. Soybean foods and their benefits: potential mechanisms of action. Nutrition Rev 63:272-83.
72. Park JH, Jeong HJ, De Lumen BO. 2005. Contents and bioactivities of lunasin, Bowman-Birk inhibitor, and isoflavones in soybean seed. J Agr Food Chem 53:7686-90.
73. Park JH, Jeong HJ, Lumen BOD. 2007. In vitro digestibility of the cancer-preventive soy peptides lunasin and BBI. J Agr Food Chem 55:10703-6.
74. Persky VW, Turyk ME, Wang L, Freels S, Chatterton R, Barnes S, Potter S. 2002. Effect of soy protein on endogenous hormones in postmenopausal women. Am J Clin Nutr 75:145-53.
75. Rizzello CG, Nionelli L, Coda R, Gobbetti M. 2012. Synthesis of the cancer preventive peptide lunasin by lactic acid bacteria during sourdough fermentation. Nutr Cancer 64:111-20.
76. Sacks FM, Lichtenstein A, Van Horn L, Harris W, Kris-Etherton P, Winston M. 2006. Soy protein, isoflavones, and cardiovascular health an American Heart Association science advisory for professionals from the nutrition committee. Circulation 113:1034-44.
77. Sagara M, Kanda T, NJelekera M, Teramoto T, Armitage L, Birt N, Birt C, Yamori Y. 2004. Effects of dietary intake of soy protein and isoflavones on cardiovascular disease risk factors in high risk, middle-aged men in Scotland. J Am Coll Nutr 23:85-91.
78. Seber LE, Barnett BW, McConnell EJ, Hume SD, Cai J, Boles K, Davis KR. 2012. Scalable purification and characterization of the anticancer lunasin peptide from soybean. PloS one 7:e35409.
79. Setrerrahmane S, Zhang Y, Dai G, Lv J, Tan S. 2014. Efficient production of native lunasin with correct N-terminal processing by using the pH-induced self-cleavable Ssp dnab mini-intein system in Escherichia coli. Appl Biochem Biotech 174:612-622.
80. Silva-Sanchez C, De la Rosa AB, Leon-Galvan MF, De Lumen, BO, De Leon-Rodriguez A, de Mejia EG. 2008. Bioactive peptides in amaranth (Amaranthus hypochondriacus) seed. J Agr Food Chem 56:1233-40.
81. Sirtori CR, Even R, Lovati MR. 1993. Soybean protein diet and plasma cholesterol: from therapy to molecular mechanisms. Ann N Y Acad Sci 676:188-201.
82. TM. Available from: www.soylabs.com/Products.aspx. Accessed July 21, 2014.
83. Tikkanen MJ, Adlercreutz H. 2000. Dietary soy-derived isoflavone phytoestrogens: could they have a role in coronary heart disease prevention? Biochem Pharmacol 60:1-5.
84. Tsai TH, Tsai PJ, Ho SC. 2005. Antioxidant and anti-inflammatory activities of several commonly used spices. J food Sci 70:C93-7.
85. Tung CY, Flores S, Han L, Yao S, Zhou B, Sun J, Chang HC. 2014. Activation of dendritic cells by soypeptide lunasin as a novel vaccine adjuvant (VAC6P. 942). J Immunol 192:140-3.
86. Udenigwe CC, Aluko RE. 2011. Hypolipidemic and hypocholesterolemic food proteins and peptides. In: Hettiarachchy N, Sato K, Marshall MR. (Eds.), Bioactive food proteins and peptides: Applications in human health (pp. 191-218). New York: CRC Press.
87. Villegas I, Sanchez-Fidalgo S, Alarcon de la Lastra C. 2008. New mechanisms and therapeutic potential of curcumin for colorectal cancer. Mol Nutr Food Res 52:1040-61.
88. Wang Y, Jones PJ, Ausman LM, Lichtenstein AH. 2004. Soy protein reduces triglyceride levels and triglyceride fatty acid fractional synthesis rate in hypercholesterolemic subjects. Atherosclerosis 173:269-75.
89. Wang W, Dia VP, Vasconez M, De Mejia EG, Nelson RL. 2008. Analysis of soybean protein-derived peptides and the effect of cultivar, environmental conditions, and processing on lunasin concentration in soybean and soy products. J AOAC Int 91:936-46.
90. Wu AH, Ziegler RG, Nomura AM, West DW, Kolonel LN, Horn-Ross PL, Hoover RN, Pike MC. 1998. Soy intake and risk of breast cancer in Asians and Asian Americans. Am J Clin Nutr 68:1437S-43S.
91. Yamamoto S, Sobue T, Kobayashi M, Sasaki S, Tsugane S. 2003. Soy, isoflavones, and breast cancer risk in Japan. J Natl Cancer I 95:906-13.
92. Zhang X, Shu XO, Gao YT, Yang G, Li Q, Li H, Jin H, Zheng W. 2003. Soy food consumption is associated with lower risk of coronary heart disease in Chinese women. J Nutr 133:2874-8.
93. Zhong F, Liu J, Ma J, Shoemaker CF. 2007. Preparation of hypocholesterol peptides from soy protein and their hypocholesterolemic effect in mice. Food Res Int 40:661-7.