Efficient production of native lunasin with correct n-terminal processing by using the pH-induced self-cleavable Ssp DnaB mini-intein system in Escherichia coli

Applied Biochemistry and Biotechnology

Volumn 174, Issue 2, 2014, Pages 612-622

  Setrerrahmane, Sarra ^a   Zhang, Yi ^a   Dai, Guangzhi ^a   Lv, Jing ^a   Tan, Shuhua ^a  

^a CHINA PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Chitin binding domain; Fusion protein; Lunasin; Mini intein

Indexed keywords

CLONING; ESCHERICHIA COLI; GENES; MASS SPECTROMETRY; PROTEINS;

CHITIN BINDING DOMAINS; COST-EFFECTIVE APPROACH; ESCHERICHIA COLI BL21; FUSION PROTEINS; INHIBITORY ACTIVITY; LUNASIN; MATRIX ASSISTED LASER DESORPTION; MINI-INTEIN;

CHITIN;

DNAB HELICASE; DNAB PROTEIN, E COLI; GM2S-1 PROTEIN, GLYCINE MAX; INTEIN; PRIMER DNA; SOYBEAN PROTEIN;

BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

BASE SEQUENCE; DNA PRIMERS; DNAB HELICASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; INTEINS; POLYMERASE CHAIN REACTION; SOYBEAN PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84906948427     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-014-1081-1     Document Type: Article

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