Scalable purification and characterization of the anticancer lunasin peptide from soybean

PLoS ONE

Volumn 7, Issue 4, 2012, Pages

  Seber, Lauren E ^a,b   Barnett, Brian W ^b,e   McConnell, Elizabeth J ^b   Hume, Steven D ^c   Cai, Jian ^d   Boles, Kati ^b   Davis, Keith R ^a,b,d  

^a UNIVERSITY OF LOUISVILLE   (United States)

^b Owensboro Cancer Research Program   (United States)

^c Kentucky BioProcessing   (United States)

^d University of Louisville School of Medicine   (United States)

^e BASF CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTINEOPLASTIC AGENT; ASPARAGINE; HISTONE; HISTONE H3; HISTONE H4; LUNASIN; SOYBEAN PROTEIN; UNCLASSIFIED DRUG; GM2S 1 PROTEIN, GLYCINE MAX; GM2S-1 PROTEIN, GLYCINE MAX; PEPTIDE;

AMINO ACID SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ANTIINFLAMMATORY ACTIVITY; ANTINEOPLASTIC ACTIVITY; ARTICLE; BINDING ASSAY; CARBOXY TERMINAL SEQUENCE; DRUG ACTIVITY; DRUG PURIFICATION; DRUG PURITY; DRUG STRUCTURE; ELECTROSPRAY MASS SPECTROMETRY; ELUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; HISTONE ACETYLATION; IMMUNOBLOTTING; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; REVERSED PHASE LIQUID CHROMATOGRAPHY; SOYBEAN; STRUCTURE ANALYSIS; ULTRAFILTRATION; CHEMISTRY; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; TANDEM MASS SPECTROMETRY;

ANIMALIA; GLYCINE MAX;

ANTINEOPLASTIC AGENTS; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HISTONES; HUMANS; PEPTIDES; PROTEIN BINDING; SOYBEAN PROTEINS; SOYBEANS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 84859719895     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0035409     Document Type: Article

References (35)

1. Odani S, Koide T, Ono T, (1987) Amino acid sequence of a soybean (Glycine max) seed polypeptide having a poly(L-aspartic acid) structure. J Biol Chem 262: 10502-10505.
2. Galvez AF, de Lumen BO, (1999) A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nat Biotechnol 17: 495-500.
3. de Mejia E, de Lumen B, (2006) Soybean bioactive peptides: A new horizon in preventing chronic diseases. Sex Reprod & Menopause 4: 91-95.
4. Omoni AO, Aluko RE, (2005) Soybean foods and their benefits: potential mechanisms of action. Nutrition Reviews 63: 272-283.
5. Galvez AF, Chen N, Macasieb J, de Lumen BO, (2001) Chemopreventive property of a soybean peptide (lunasin) that binds to deacetylated histones and inhibits acetylation. Cancer Res 61: 7473-7478.
6. Hernandez-Ledesma B, Hsieh CC, de Lumen BO, (2009) Lunasin, a novel seed peptide for cancer prevention. Peptides 30: 426-430.
7. Jeong HJ, Lam Y, de Lumen BO, (2002) Barley lunasin suppresses ras-induced colony formation and inhibits core histone acetylation in mammalian cells. J Agric Food Chem 50: 5903-5908.
8. Lam Y, Galvez A, de Lumen BO, (2003) Lunasin suppresses E1A-mediated transformation of mammalian cells but does not inhibit growth of immortalized and established cancer cell lines. Nutr Cancer 47: 88-94.
9. Jeong HJ, Jeong JB, Kim DS, Park JH, Lee JB, et al. (2007) The cancer preventive peptide lunasin from wheat inhibits core histone acetylation. Cancer Lett 255: 42-48.
10. Jeong JB, de Lumen BO, Jeong HJ, (2010) Lunasin peptide purified from Solanum nigrum L. protects DNA from oxidative damage by suppressing the generation of hydroxyl radical via blocking fenton reaction. Cancer Lett 293: 58-64.
11. Jeong JB, Jeong HJ, Park JH, Lee SH, Lee JR, et al. (2007) Cancer-preventive peptide lunasin from Solanum nigrum L. inhibits acetylation of core histones H3 and H4 and phosphorylation of retinoblastoma protein (Rb). J Agric Food Chem 55: 10707-10713.
12. Maldonado-Cervantes E, Jeong HJ, Leon-Galvan F, Barrera-Pacheco A, de Leon-Rodriguez A, et al. (2010) Amaranth lunasin-like peptide internalizes into the cell nucleus and inhibits chemical carcinogen-induced transformation of NIH-3T3 cells. Peptides 31: 1635-1642.
13. Silva-Sanchez C, de la Rosa AP, Leon-Galvan MF, de Lumen BO, de Leon-Rodriguez A, et al. (2008) Bioactive peptides in amaranth (Amaranthus hypochondriacus) seed. J Agric Food Chem 56: 1233-1240.
14. de Mejia EG, Vasconez M, de Lumen BO, Nelson R, (2004) Lunasin concentration in different soybean genotypes, commercial soy protein, and isoflavone products. J Agric Food Chem 52: 5882-5887.
15. Wang W, Dia VP, Vasconez M, de Mejia EG, Nelson RL, (2008) Analysis of soybean protein-derived peptides and the effect of cultivar, environmental conditions, and processing on lunasin concentration in soybean and soy products. J AOAC Int 91: 936-946.
16. de Mejia EG, Wang W, Dia VP, (2010) Lunasin, with an arginine-glycine-aspartic acid motif, causes apoptosis to L1210 leukemia cells by activation of caspase-3. Mol Nutr Food Res 54: 406-414.
17. Dia VP, Mejia EG, (2010) Lunasin promotes apoptosis in human colon cancer cells by mitochondrial pathway activation and induction of nuclear clusterin expression. Cancer Lett 295: 44-53.
18. Hsieh CC, Hernandez-Ledesma B, de Lumen BO, (2010) Lunasin, a novel seed peptide, sensitizes human breast cancer MDA-MB-231 cells to aspirin-arrested cell cycle and induced apoptosis. Chem Biol Interact 186: 127-134.
19. Hsieh CC, Hernandez-Ledesma B, Jeong HJ, Park JH, de Lumen BO, (2010) Complementary roles in cancer prevention: protease inhibitor makes the cancer preventive peptide lunasin bioavailable. PLoS One 5: e8890.
20. de Mejia EG, Dia VP, (2009) Lunasin and lunasin-like peptides inhibit inflammation through suppression of NF-kappaB pathway in the macrophage. Peptides 30: 2388-2398.
21. Dia VP, Berhow MA, de Mejia EG, (2008) Bowman-Birk inhibitor and genistein among soy compounds that synergistically inhibit nitric oxide and prostaglandin E2 pathways in lipopolysaccharide-induced macrophages. J Agric Food Chem 56: 11707-11717.
22. Hernandez-Ledesma B, Hsieh CC, de Lumen BO, (2009) Antioxidant and anti-inflammatory properties of cancer preventive peptide lunasin in RAW 264.7 macrophages. Biochem Biophys Res Commun 390: 803-808.
23. Galvez AF, Huang L, Magbanua MM, Dawson K, Rodriguez RL, (2011) Differential expression of thrombospondin (THBS1) in tumorigenic and nontumorigenic prostate epithelial cells in response to a chromatin-binding soy peptide. Nutr Cancer 63: 623-636.
24. Hernandez-Ledesma B, Hsieh CC, de Lumen BO, (2011) Relationship between lunasin's sequence and its inhibitory activity of histones H3 and H4 acetylation. Mol Nutr Food Res 55: 989-998.
25. Jeong HJ, Jeong JB, Kim DS, de Lumen BO, (2007) Inhibition of core histone acetylation by the cancer preventive peptide lunasin. J Agric Food Chem 55: 632-637.
26. de Lumen BO, (2005) Lunasin: a cancer-preventive soy peptide. Nutr Rev 63: 16-21.
27. Dia VP, de Mejia EG, (2011) Lunasin induces apoptosis and modifies the expression of genes associated with extracellular matrix and cell adhesion in human metastatic colon cancer cells. Mol Nutr Food Res 55: 623-634.
28. Dia VP, de Mejia EG, (2011) Lunasin potentiates the effect of oxaliplatin preventing outgrowth of colon cancer metastasis, binds to alpha5beta1 integrin and suppresses FAK/ERK/NF-kappaB signaling. Cancer Lett 313: 167-180.
29. Dia VP, de Mejia EG, (2011) Differential gene expression of RAW 264.7 macrophages in response to the RGD peptide lunasin with and without lipopolysaccharide stimulation. Peptides 32: 1979-1988.
30. Dia VP, Wang W, Oh VL, de Lumen BO, de Mejia EG, (2009) Isolation, purification and characterisation of lunasin from defatted soybean flour and in vitro evaluation of its anti-inflammatory activity. Food Chemistry 114: 108-115.
31. Jeong HJ, Park JH, Lam Y, de Lumen BO, (2003) Characterization of lunasin isolated from soybean. J Agric Food Chem 51: 7901-7906.
32. Lin J, Fido R, Shewry P, Archer DB, Alcocer MJ, (2004) The expression and processing of two recombinant 2S albumins from soybean (Glycine max) in the yeast Pichia pastoris. Biochim Biophys Acta 1698: 203-212.
33. Ampe C, van Damme J, de Castro LA, Sampaio MJ, van Montagu M, et al. (1986) The amino-acid sequence of the 2S sulphur-rich proteins from seeds of Brazil nut (Bertholletia excelsa H.B.K.). Eur J Biochem 159: 597-604.
34. Ericson ML, Rodin J, Lenman M, Glimelius K, Josefsson LG, et al. (1986) Structure of the rapeseed 1.7 S storage protein, napin, and its precursor. J Biol Chem 261: 14576-14581.
35. Krebbers E, Herdies L, de Clercq A, Seurinck J, Leemans J, et al. (1988) Determination of the processing sites of an Arabidopsis 2S albumin and characterization of the complete gene family. Plant Physiol 87: 859-866.