Experimental and computational mutagenesis to investigate the positioning of a general base within an enzyme active site

Biochemistry

Volumn 53, Issue 15, 2014, Pages 2541-2555

  Schwans, Jason P ^a   Hanoian, Philip ^b   Lengerich, Benjamin J ^b   Sunden, Fanny ^a   Gonzalez, Ana ^d   Tsai, Yingssu ^d,e   Hammes Schiffer, Sharon ^c   Herschlag, Daniel ^a  

^a STANFORD UNIVERSITY   (United States)

^b PENNSYLVANIA STATE UNIVERSITY   (United States)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^d SLAC NATIONAL ACCELERATOR LABORATORY   (United States)

^e STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGY; CARBOXYLATION; CATALYSIS; MOLECULAR DYNAMICS; OPTIMIZATION; OXYGEN; PROTEINS; X RAY CRYSTALLOGRAPHY;

COMPUTATIONAL MUTAGENESIS; GENERAL BASE CATALYSIS; KETOSTEROID ISOMERASE; MOLECULAR DYNAMICS SIMULATIONS; MULTIPLE INTERACTIONS; OPTIMAL POSITIONING; PROTEIN ARCHITECTURES; STRUCTURAL INSPECTIONS;

ENZYMES;

ASPARTIC ACID; CARBOXYLIC ACID; GLUTAMIC ACID; GLYCINE; MUTANT PROTEIN; OXYGEN; PROLINE; SERINE; STEROID DELTA ISOMERASE; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME STRUCTURE; HYDROPHOBICITY; ISOMERIZATION; MOLECULAR DYNAMICS; MUTAGENESIS; MUTANT; PRIORITY JOURNAL; QUANTUM CHEMISTRY; WILD TYPE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; AMINO ACIDS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; MOLECULAR DYNAMICS SIMULATION; MOLECULAR SEQUENCE DATA; MUTAGENESIS; STEROID ISOMERASES;

EID: 84899463140     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi401671t     Document Type: Article

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