Crystal structure and enzyme mechanism of δ5-3-ketosteroid isomerase from Pseudomonas testosteroni

Biochemistry

Volumn 37, Issue 23, 1998, Pages 8325-8330

  Cho, Hyun Soo ^a   Choi, Gildon ^a   Choi, Kwan Yong ^a   Oh, Byung Ha ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

STEROID DELTA ISOMERASE;

ARTICLE; COMAMONAS TESTOSTERONI; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

BINDING SITES; CATALYSIS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; HYDROGEN BONDING; MODELS, MOLECULAR; PHENYLALANINE; PSEUDOMONAS; STEROID ISOMERASES; STRUCTURE-ACTIVITY RELATIONSHIP;

BACTERIA (MICROORGANISMS); COMAMONAS TESTOSTERONI; PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 0032499672     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9801614     Document Type: Article

References (30)

1. Batzold, F. H., Benson, A. M., Covey, D. F., Robinson, C. H., and Talalay, P. (1976) Adv. Enzyme Regul. 14, 243-267.
2. Radzicka, A., and Wolfenden, R. (1995) Science 267, 90-93.
3. Xue, L., Kuliopulos, A., Mildvan, A. S., and Talalay, P. (1991) Biochemistry 30, 4991-4997.
4. Xue, L., Talalay, P., and Mildvan, A. S. (1991) Biochemistry 30, 10858-10865.
5. Holman, C. M., and Benisek, W. F. (1994) Biochemistry 33, 2672-2681.
6. Hawkinson, D. C., Pollack, R. M., and Ambulos, N. P., Jr. (1994) Biochemistry 33, 12172-12183.
7. Hawkinson, D. C., Eames, T. C., and Pollack, R. M. (1991) Biochemistry 30, 10849-10858.
8. Shan, S., Loh, S., and Herschlag, D. (1996) Science 272, 97-101.
9. Wu, Z. R., Ebrahimian, S., Zawrotny, M. E., Thornburg, L. D., Perez-Alvarado, G. C., Brothers, P., Pollack, R. M., and Summers, M. F. (1997) Science 276, 415-418.
10. Kim, S. W., Cha, S.-S., Cho, H.-S., Kim, J.-S., Ha, N.-C., Cho, M.-J., J., S., Kim, K.-K., Choi, K. Y., and Oh, B.-H. (1997) Biochemistry 36, 14030-14036.
11. Kim, S. W., Kim, C. Y., Benisek, W. F., and Choi, K. Y. (1994) J. Bacteriol. 176, 6672-6676.
12. Brothers, P. N., Blotny, G., Qi, L., and Pollack, R. M. (1995) Biochemistry 34, 15453-15458.
13. Zhao, Q., Abeygunawardana, C., Talalay, P., and Mildvan, A. S. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 8220-8224.
14. Gerlt, J. A., and Gassman, P. G. (1993) J. Am. Chem. Soc. 115, 11552-11568.
15. Gerlt, J. A., and Gassman, P. G. (1993) Biochemistry 32, 11943-11952.
16. Cleland, W. W., and Kreevoy, M. M. (1994) Science 264, 1887-1890.
17. Frey, P. A., Whitt, S. A., and Tobin, J. B. (1994) Science 264, 1927-1930.
18. Zhao, Q., Abeygunawardana, C., Gittis, A. G., and Mildvan, A. S. (1997) Biochemistry 36, 14616-14626.
19. Kim, S. W., and Choi, K. Y. (1995) J. Bacteriol. 177, 2602-2605.
20. Otwinowski, Z. (1993) in Proceedings of the CCP4 Study Weekend (Sawyer, L., et al., Eds.) pp 56-62, SERC Daresbury Laboratory, Warrington, U.K.
21. Brünger, A. T. (1992) X-PLOR Version 3.0, Yale University, New Haven, CT.
22. Westbrook, E. M., Piro, O. E., and Sigler, P. B. (1984) J. Biol. Chem. 259, 9096-9103.
23. Westbrook, E. M. (1976) J. Mol. Biol. 103, 659-664.
24. Westbrook, E. M., Sigler, P. B., Berman, H., Glusker, J. P., Bunick, G., Benson, A., and Talalay, P. (1976) J. Mol. Biol. 103, 665-667.
25. Weintraub, H., Alfsen, A., and Baulieu, E.-E. (1970) Eur. J. Biochem. 12, 217-221.
26. Li, Y. K., Kuliopulos, A., Mildvan, A. S., and Talalay, P. (1993) Biochemistry 32, 1816-1824.
27. Kuliopulos, A., Mildvan, A. S., Shortle, D., and Talalay, P. (1989) Biochemistry 28, 149-159.
28. Zeng, B., and Pollack, R. M. (1991) J. Am. Chem. Soc. 113, 3838-3842.
29. Jones, T. A., and Kjeldgaard, M. (1993) O version 5.9. The Manual. Uppsala University, Uppsala, Sweden.
30. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950.