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Volumn 40, Issue 23, 2001, Pages 6828-6835
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Pseudoreversion of the catalytic activity of Y14F by the additional substitution(s) of tyrosine with phenylalanine in the hydrogen bond network of Δ5-3-ketosteroid isomerase from Pseudomonas putida biotype B
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Author keywords
[No Author keywords available]
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Indexed keywords
PROTON-TRANSFER MECHANISM;
PSEUDOREVERSION;
CATALYST ACTIVITY;
CRYSTAL STRUCTURE;
ENZYME KINETICS;
HYDROGEN BONDS;
ISOMERIZATION;
BIOCHEMISTRY;
DEUTERIUM;
PHENYLALANINE;
STEROID DELTA ISOMERASE;
TYROSINE;
AMINO ACID SUBSTITUTION;
ARTICLE;
CATALYSIS;
COMPARATIVE STUDY;
CONTROLLED STUDY;
CRYSTAL STRUCTURE;
ENZYME ACTIVE SITE;
ENZYME ANALYSIS;
GENE MUTATION;
HYDROGEN BOND;
ISOMERISM;
NONHUMAN;
PRIORITY JOURNAL;
PROTON TRANSPORT;
PSEUDOMONAS PUTIDA;
REACTION ANALYSIS;
STRUCTURE ANALYSIS;
ACRYLAMIDE;
AMINO ACID SUBSTITUTION;
ANDROSTENEDIONE;
CATALYSIS;
CRYSTALLOGRAPHY, X-RAY;
DEUTERIUM OXIDE;
ENZYME ACTIVATION;
HYDROGEN BONDING;
KINETICS;
MUTAGENESIS, SITE-DIRECTED;
PHENYLALANINE;
PSEUDOMONAS PUTIDA;
SPECTROMETRY, FLUORESCENCE;
STEROID ISOMERASES;
STRUCTURE-ACTIVITY RELATIONSHIP;
SUBSTRATE SPECIFICITY;
TRYPTOPHAN;
TYROSINE;
BACTERIA (MICROORGANISMS);
PSEUDOMONAS;
PSEUDOMONAS PUTIDA;
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EID: 0035849571
PISSN: 00062960
EISSN: None
Source Type: Journal
DOI: 10.1021/bi002767+ Document Type: Article |
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Times cited : (26)
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References (35)
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