The conserved cis-Pro39 residue plays a crucial role in the proper positioning of the catalytic base Asp38 in ketosteroid isomerase from Comamonas testosteroni

Biochemical Journal

Volumn 375, Issue 2, 2003, Pages 297-305

  Nam, Gyu Hyun ^a,b   Cha, Sun Shin ^b   Yun, Young Sung ^a,b   Yun Hee, O H ^a,b   Hong, Bee Hak ^a,b   Lee, Heung Soo ^b   Choi, Kwan Yong ^a,b  

^a Natl Res Lab Protein Folding E   (South Korea)

^b Pohang University of Science and Technology (POSTECH)   (South Korea)

Author keywords

Active site geometry; cis proline; Ketosteroid isomerase; Site directed mutagenesis; Structural integrity

Indexed keywords

ACTIVATION ENERGY; CATALYSIS; ENZYMES; FREE ENERGY; ISOMERIZATION; ISOMERS;

STRUCTURAL RIGIDITY;

BIOCHEMISTRY;

8 ANILINO 1 NAPHTHALENESULFONIC ACID; ALANINE; EQUILENIN; GLYCINE; PROLINE; SERINE; STEROID DELTA ISOMERASE; UREA; VALINE; 5-ANDROSTENE-3,17-DIONE; ANDROST 5 ENE 3,17 DIONE; ANDROSTENEDIONE; ASPARTIC ACID;

ARTICLE; CATALYSIS; CATALYST; COMAMONAS TESTOSTERONI; CONTROLLED STUDY; CRYSTAL STRUCTURE; GENE MUTATION; GEOMETRY; HYDROPHOBICITY; MUTANT; NONHUMAN; PRIORITY JOURNAL; RIGIDITY; WILD TYPE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MUTATION; NUCLEOTIDE SEQUENCE; PH; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; STRUCTURE ACTIVITY RELATION; THERMODYNAMICS; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); COMAMONAS TESTOSTERONI;

ANDROSTENEDIONE; ASPARTIC ACID; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; COMAMONAS TESTOSTERONI; CONSERVED SEQUENCE; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; EQUILENIN; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTATION; PROLINE; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; STEROID ISOMERASES; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

EID: 0142200482     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030263     Document Type: Article

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