Roles of active site aromatic residues in catalysis by ketosteroid isomerase from Pseudomonas putida biotype B

Biochemistry

Volumn 38, Issue 42, 1999, Pages 13810-13819

  Kim, Do Hyung ^a   Nam, Gyu Hyun ^a   Jang, Do Soo ^a   Choi, Gildon ^a   Joo, Soyoung ^a   Kim, Jeong Sun ^a   Oh, Byung Ha ^a   Choi, Kwan Yong ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC COMPOUNDS; BINDING ENERGY; ENZYME KINETICS; MUTAGENESIS; PHASE EQUILIBRIA; REACTION KINETICS; SUBSTITUTION REACTIONS;

AROMATIC RESIDUES; EQUILIBRIUM BINDING ANALYSIS; KETOSTEROID ISOMERASE; PSEUDOMONAS PUTIDA;

CATALYSIS;

AROMATIC AMINO ACID; BACTERIAL ENZYME; NANDROLONE; PHENYLALANINE; STEROID DELTA ISOMERASE; TRYPTOPHAN;

ARTICLE; BINDING KINETICS; CATALYSIS; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME MODIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA; STRUCTURE ACTIVITY RELATION;

ALANINE; AMINO ACID SUBSTITUTION; BINDING SITES; CATALYSIS; EQUILENIN; KINETICS; MUTAGENESIS, SITE-DIRECTED; NANDROLONE; PHENYLALANINE; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; STEROID ISOMERASES; TRYPTOPHAN; TYROSINE;

EID: 0344564131     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi991040m     Document Type: Article

References (41)

1. Batzold, F. H., Benson, A. M., Covey, D. F., Robinson, C. H., and Talalay, P. (1976) Adv. Enzyme Regul. 14, 243-267.
2. Schwab, J. M., and Henderson, B. S. (1990) Chem. Rev. 90, 1203-1245.
3. Xue, L., Talalay, P., and Mildvan, A. S. (1990) Biochemistry 29, 7491-7500.
4. Kuliopulos, A., Mullen, G. P., Xue, L., amd Mildvan, A. S. (1991) Biochemistry 30, 3169-3178.
5. Wang, S.-F., Kawahara, F. S., and Talalay, P. (1963) J. Biol. Chem. 238, 576-585.
6. Pollack, R. M., Mack, J. P. G., and Eldin, S. (1987) J. Am. Chem. Soc. 109, 5048-5050.
7. Xue, L., Kuliopulos, A., Mildvan, A. S., and Talalay, P. (1991) Biochemistry 30, 4991-4997.
8. Hawkinson, D. C., Eames, T. C., and Pollack, R. M. (1991) Biochemistry 30, 10849-10858.
9. Hawkinson, D. C., Pollack, R. M., and Ambulos, N. P., Jr. (1994) Biochemistry 33, 12172-12183.
10. Kuliopulos, A., Talalay, P., and Mildvan, A. S. (1990) Biochemistry 29, 10271-10280.
11. Kim, S. W., Cha, S.-S., Cho, H.-S., Kim, J.-S., Ha, N.-C., Cho, M.-J., Joo, S., Kim, K.-K., Choi, K. Y., and Oh, B.-H. (1997) Biochemistry 36, 14030-14036.
12. Cho, H.-S., Choi, G., Choi, K. Y., and Oh, B.-H. (1998) Biochemistry 37, 8325-8330.
13. Wu, Z. R., Ebrahimian, S., Zawrotny, M. E., Thornburg, L. D., Perez-Alvarado, G. C., Brothers, P., Pollack, R. M., and Summers, M. F. (1997) Science 276, 415-418.
14. Massiah, M. A., Abeygunawardana, C., Gittis, A. G., and Mildvan, A. S. (1998) Biochemistry 37, 14701-14712.
15. Xue, L., Talalay, P., and Mildvan, A. S. (1991) Biochemistry 30, 10858-10865.
16. Kim, S. W., Joo, S., Choi, G., Cho, H.-S., Oh, B.-H., and Choi, K. Y. (1997) J. Bacteriol. 179, 7742-7747.
17. Gerlt, J. A., and Gassman, P. G. (1993) J. Am. Chem. Soc. 115, 11552-11568.
18. Zhao, Q., Abeygunawardana, C., Talalay, P., and Milvan, A. S. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 8220-8224.
19. Zhao, Q., Abeygunawardana, C., and Mildvan, A. S. (1997) Biochemistry 36, 3458-3472.
20. Zhao, Q., Abeygunawardana, C., Gittis, A. G., and Mildvan, A. S. (1997) Biochemistry 36, 14616-14626.
21. Brothers, P. N., Blotny, G., Qi, L., and Pollack, R. M. (1995) Biochemistry 34, 15453-15458.
22. Qi, L., and Pollack, R. M. (1998) Biochemistry 37, 6760-6766.
23. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 488-492.
24. Kim, S. W., and Choi, K. Y. (1995) J. Bacteriol. 177, 2602-2605.
25. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5463-5467.
26. Kim, S. W., and Choi, K. Y. (1994) J. Bacteriol. 176, 6672-6676.
27. Connolly, M. L. (1993) J. Mol. Graphics 11, 139-141.
28. Otwinoski, Z., and Minor, W. (1997) Methods Enzymol. 276, 307-326.
29. Brunger, A. T. (1992) X-PLOR Version 3.0, Yale University Press, New Haven, CT.
30. Holman, C., and Benisek, W. (1994) Biochemistry 33, 2672-2681.
31. Holman, C., and Benisek, W. (1995) Biochemistry 34, 14245-14253.
32. Kuliopulos, A., Mildvan, A. S., Shortle, D., and Talalay, P. (1989) Biochemistry 28, 149-159.
33. Hawkinson, D. C., and Pollack, R. M. (1993) Biochemistry 32, 694-698.
34. Bevins, C. L., Pollack, R. M., Kayser, R. L., and Bounds, P. L. (1986) Biochemistry 25, 5159-5164.
35. Sack, J. S. (1988) J. Mol. Graphics 6, 224-225.
36. Kiaulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950.
37. Luzzati, P. V. (1952) Acta Crystallogr. 5, 802-810.
38. Fersht, A. (1985) in Enzyme Structure and Mechanism, 2nd ed., pp 294-346, W. H. Freeman and Co., New York.
39. Carrell, H. L., Glusker, J. P., Covey, D. F., Batzold, F. H., and Robinson, C. H. (1978) J. Am. Chem. Soc. 100, 4282-4289.
40. Bhadbhade, M. M., and Venkatesan, K. (1984) Acta Crystallogr., Sect. C (Crystal Struct. Commun.) 40, 1905.
41. Busetta, B., Comberton, G., Courseille, C., and Hospital, M. (1987) Crystal Struct. Commun. 1, 129.