Biology and Mechanisms of Short RNAs in Caenorhabditis elegans

Advances in Genetics

Volumn 83, Issue , 2013, Pages 1-69

  Grishok, Alla ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84880723481     PISSN: 00652660     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-407675-4.00001-8     Document Type: Chapter

References (229)

1. Abbott A.L. Uncovering new functions for microRNAs in Caenorhabditis elegans. Current Biology 2011, 21(17):R668-R671.
2. Abbott A.L., Alvarez-Saavedra E., Miska E.A., Lau N.C., Bartel D.P., Horvitz H.R., et al. The let-7 MicroRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans. Developmental Cell 2005, 9(3):403-414.
3. Alcazar R.M., Lin R., Fire A.Z. Transmission dynamics of heritable silencing induced by double-stranded RNA in Caenorhabditis elegans. Genetics 2008, 180(3):1275-1288.
4. Alvarez-Saavedra E., Horvitz H.R. Many families of C. elegans microRNAs are not essential for development or viability. Current Biology 2010, 20(4):367-373.
5. Ambros V. MicroRNAs and developmental timing. Current Opinion in Genetics and Development 2011, 21(4):511-517.
6. Ambros V., Lee R.C., Lavanway A., Williams P.T., Jewell D. MicroRNAs and other tiny endogenous RNAs in C. elegans. Current Biology 2003, 13(10):807-818.
7. Aoki K., Moriguchi H., Yoshioka T., Okawa K., Tabara H. In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans. EMBO Journal 2007, 26(24):5007-5019.
8. Ashe A., Sapetschnig A., Weick E.M., Mitchell J., Bagijn M.P., Cording A.C., et al. piRNAs can trigger a multigenerational epigenetic memory in the germline of C. elegans. Cell 2012, 150(1):88-99.
9. Asikainen S., Storvik M., Lakso M., Wong G. Whole genome microarray analysis of C. elegans rrf-3 and eri-1 mutants. FEBS Letters 2007, 581(26):5050-5054.
10. Avgousti D.C., Palani S., Sherman Y., Grishok A. CSR-1 RNAi pathway positively regulates histone expression in C. elegans. EMBO Journal 2012, 31(19):3821-3832.
11. Bagijn M.P., Goldstein L.D., Sapetschnig A., Weick E.M., Bouasker S., Lehrbach N.J., et al. Function, targets, and evolution of Caenorhabditis elegans piRNAs. Science 2012, 337(6094):574-578.
12. Bartel D.P. MicroRNAs: Target recognition and regulatory functions. Cell 2009, 136(2):215-233.
13. Batista P.J., Ruby J.G., Claycomb J.M., Chiang R., Fahlgren N., Kasschau K.D., et al. PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans. Molecular Cell 2008, 31:67-78.
14. Billi A.C., Alessi A.F., Khivansara V., Han T., Freeberg M., Mitani S., et al. The Caenorhabditis elegans HEN1 ortholog, HENN-1, methylates and stabilizes select subclasses of germline small RNAs. PLoS Genetics 2012, 8(4):e1002617.
15. Billi A.C., Freeberg M.A., Day A.M., Chun S.Y., Khivansara V., Kim J.K. A conserved upstream motif orchestrates autonomous, germline-enriched expression of Caenorhabditis elegans piRNAs. PLoS Genetics 2013, 9(3):e1003392.
16. Billi A.C., Freeberg M.A., Kim J.K. piRNAs and siRNAs collaborate in Caenorhabditis elegans genome defense. Genome Biology 2012, 13(7):164.
17. Blanchard D., Parameswaran P., Lopez-Molina J., Gent J., Saynuk J.F., Fire A. On the nature of in vivo requirements for rde-4 in RNAi and developmental pathways in C. elegans. RNA Biology 2011, 8(3):458-467.
18. Boehm M., Slack F. A developmental timing microRNA and its target regulate life span in C. elegans. Science 2005, 310(5756):1954-1957.
19. Bosher J.M., Dufourcq P., Sookhareea S., Labouesse M. RNA interference can target pre-mRNA: Consequences for gene expression in a Caenorhabditis elegans operon. Genetics 1999, 153(3):1245-1256.
20. Bouasker S., Simard M.J. The slicing activity of miRNA-specific Argonautes is essential for the miRNA pathway in C. elegans. Nucleic Acids Research 2012, 40(20):10452-10462.
21. Boulias K., Horvitz H.R. The C. elegans microRNA mir-71 acts in neurons to promote germline-mediated longevity through regulation of DAF-16/FOXO. Cell Metabolism 2012, 15(4):439-450.
22. Brenner J.L., Jasiewicz K.L., Fahley A.F., Kemp B.J., Abbott A.L. Loss of individual microRNAs causes mutant phenotypes in sensitized genetic backgrounds in C. elegans. Current Biology 2010, 20(14):1321-1325.
23. Brenner J.L., Kemp B.J., Abbott A.L. The mir-51 family of microRNAs functions in diverse regulatory pathways in Caenorhabditis elegans. PLoS One 2012, 7(5):e37185.
24. Buckley B.A., Burkhart K.B., Gu S.G., Spracklin G., Kershner A., Fritz H., et al. A nuclear Argonaute promotes multigenerational epigenetic inheritance and germline immortality. Nature 2012, 489(7416):447-451.
25. Burkhart K.B., Guang S., Buckley B.A., Wong L., Bochner A.F., Kennedy S. A pre-mRNA-associating factor links endogenous siRNAs to chromatin regulation. PLoS Genetics 2011, 7(8):e1002249.
26. Burton N.O., Burkhart K.B., Kennedy S. Nuclear RNAi maintains heritable gene silencing in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America 2011, 108(49):19683-19688.
27. Cecere G., Zheng G.X., Mansisidor A.R., Klymko K.E., Grishok A. Promoters recognized by forkhead proteins exist for individual 21U-RNAs. Molecular Cell 2012, 47(5):734-745.
28. Ceol C.J., Horvitz H.R. A new class of C. elegans synMuv genes implicates a Tip60/NuA4-like HAT complex as a negative regulator of Ras signaling. Developmental Cell 2004, 6(4):563-576.
29. Clark A.M., Goldstein L.D., Tevlin M., Tavare S., Shaham S., Miska E.A. The microRNA miR-124 controls gene expression in the sensory nervous system of Caenorhabditis elegans. Nucleic Acids Research 2010, 38(11):3780-3793.
30. Claycomb J.M., Batista P.J., Pang K.M., Gu W., Vasale J.J., van Wolfswinkel J.C., et al. The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregation. Cell 2009, 139(1):123-134.
31. Cochella L., Hobert O. Embryonic priming of a miRNA locus predetermines postmitotic neuronal left/right asymmetry in C. elegans. Cell 2012, 151(6):1229-1242.
32. Collins J., Forbes E., Anderson P. The Tc3 family of transposable genetic elements in Caenorhabditis elegans. Genetics 1989, 121(1):47-55.
33. Conine C.C., Batista P.J., Gu W., Claycomb J.M., Chaves D.A., Shirayama M., et al. Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America 2010, 107(8):3588-3593.
34. Correa R.L., Steiner F.A., Berezikov E., Ketting R.F. MicroRNA-directed siRNA biogenesis in Caenorhabditis elegans. PLoS Genetics 2010, 6(4):e1000903.
35. Cox D.N., Chao A., Baker J., Chang L., Qiao D., Lin H. A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal. Genes & Development 1998, 12(23):3715-3727.
36. Cui M., Chen J., Myers T.R., Hwang B.J., Sternberg P.W., Greenwald I., et al. SynMuv genes redundantly inhibit lin-3/EGF expression to prevent inappropriate vulval induction in C. elegans. Developmental Cell 2006, 10(5):667-672.
37. Cui M., Kim E.B., Han M. Diverse chromatin remodeling genes antagonize the Rb-involved SynMuv pathways in C. elegans. PLoS Genetics 2006, 2(5):e74.
38. Czech B., Hannon G.J. Small RNA sorting: Matchmaking for Argonautes. Nature Reviews. Genetics 2011, 12(1):19-31.
39. Das P.P., Bagijn M.P., Goldstein L.D., Woolford J.R., Lehrbach N.J., Sapetschnig A., et al. Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline. Molecular Cell 2008, 31(1):79-90.
40. Davila Lopez M., Samuelsson T. Early evolution of histone mRNA 3' end processing. RNA 2008, 14(1):1-10.
41. de Lencastre A., Pincus Z., Zhou K., Kato M., Lee S.S., Slack F.J. MicroRNAs both promote and antagonize longevity in C. elegans. Current Biology 2010, 20(24):2159-2168.
42. Duchaine T.F., Wohlschlegel J.A., Kennedy S., Bei Y., Conte D., Pang K., et al. Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways. Cell 2006, 124(2):343-354.
43. Dudley N.R., Labbe J.C., Goldstein B. Using RNA interference to identify genes required for RNA interference. Proceedings of the National Academy of Sciences of the United States of America 2002, 99(7):4191-4196.
44. Fabian M.R., Sonenberg N., Filipowicz W. Regulation of mRNA translation and stability by microRNAs. Annual Review of Biochemistry 2010, 79:351-379.
45. Feinberg E.H., Hunter C.P. Transport of dsRNA into cells by the transmembrane protein SID-1. Science 2003, 301(5639):1545-1547.
46. Felix M.A., Ashe A., Piffaretti J., Wu G., Nuez I., Belicard T., et al. Natural and experimental infection of Caenorhabditis nematodes by novel viruses related to nodaviruses. PLoS Biology 2011, 9(1):e1000586.
47. Ferguson E.L., Horvitz H.R. The multivulva phenotype of certain Caenorhabditis elegans mutants results from defects in two functionally redundant pathways. Genetics 1989, 123(1):109-121.
48. Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 1998, 391(6669):806-811.
49. Fischer S.E. Small RNA-mediated gene silencing pathways in C. elegans. The International Journal of Biochemistry & Cell Biology 2010, 42(8):1306-1315.
50. Fischer S.E., Butler M.D., Pan Q., Ruvkun G. Trans-splicing in C. elegans generates the negative RNAi regulator ERI-6/7. Nature 2008, 455(7212):491-496.
51. Fischer S.E., Montgomery T.A., Zhang C., Fahlgren N., Breen P.C., Hwang A., et al. The ERI-6/7 helicase acts at the first stage of an siRNA amplification pathway that targets recent gene duplications. PLoS Genetics 2011, 7(11):e1002369.
52. Flamand M., Duchaine T.F. SnapShot: Endogenous RNAi machinery and mechanisms. Cell 2012, 150(3):662-662.e2.
53. Friend K., Campbell Z.T., Cooke A., Kroll-Conner P., Wickens M.P., Kimble J. A conserved PUF-Ago-eEF1A complex attenuates translation elongation. Nature Structural and Molecular Biology 2012, 19(2):176-183.
54. Gabel H.W., Ruvkun G. The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi. Nature Structural and Molecular Biology 2008, 15(5):531-533.
55. Gaydos L.J., Rechtsteiner A., Egelhofer T.A., Carroll C.R., Strome S. Antagonism between MES-4 and Polycomb repressive complex 2 promotes appropriate gene expression in C. elegans germ cells. Cell Reports 2012, 2(5):1169-1177.
56. Gent J.I., Lamm A.T., Pavelec D.M., Maniar J.M., Parameswaran P., Tao L., et al. Distinct phases of siRNA synthesis in an endogenous RNAi pathway in C. elegans soma. Molecular Cell 2010, 37(5):679-689.
57. Gent J.I., Schvarzstein M., Villeneuve A.M., Gu S.G., Jantsch V., Fire A.Z., et al. A Caenorhabditis elegans RNA-directed RNA polymerase in sperm development and endogenous RNA interference. Genetics 2009, 183(4):1297-1314.
58. Grishok A. RNAi mechanisms in Caenorhabditis elegans. FEBS Letters 2005, 579(26):5932-5939.
59. Grishok A., Hoersch S., Sharp P.A. RNA interference and retinoblastoma-related genes are required for repression of endogenous siRNA targets in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America 2008, 105(51):20386-20391.
60. Grishok A., Mello C.C. RNAi (Nematodes: Caenorhabditis elegans). Advances in Genetics 2002, 46:339-360.
61. Grishok A., Pasquinelli A.E., Conte D., Li N., Parrish S., Ha I., et al. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell 2001, 106(1):23-34.
62. Grishok A., Sharp P.A. Negative regulation of nuclear divisions in Caenorhabditis elegans by retinoblastoma and RNA interference-related genes. Proceedings of the National Academy of Sciences of the United States of America 2005, 102(48):17360-17365.
63. Grishok A., Sinskey J.L., Sharp P.A. Transcriptional silencing of a transgene by RNAi in the soma of C. elegans. Genes & Development 2005, 19(6):683-696.
64. Grishok A., Tabara H., Mello C.C. Genetic requirements for inheritance of RNAi in C. elegans. Science 2000, 287(5462):2494-2497.
65. Grosshans H., Chatterjee S. MicroRNAses and the regulated degradation of mature animal miRNAs. Advances in Experimental Medicine and Biology 2010, 700:140-155.
66. Gu W., Lee H.C., Chaves D., Youngman E.M., Pazour G.J., Conte D., et al. CapSeq and CIP-TAP identify Pol II start sites and reveal capped small RNAs as C. elegans piRNA precursors. Cell 2012, 151(7):1488-1500.
67. Gu S.G., Pak J., Barberan-Soler S., Ali M., Fire A., Zahler A.M. Distinct ribonucleoprotein reservoirs for microRNA and siRNA populations in C. elegans. RNA 2007, 13(9):1492-1504.
68. Gu S.G., Pak J., Guang S., Maniar J.M., Kennedy S., Fire A. Amplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint. Nature Genetics 2012, 44(2):157-164.
69. Gu W., Shirayama M., Conte D., Vasale J., Batista P.J., Claycomb J.M., et al. Distinct argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline. Molecular Cell 2009, 36(2):231-244.
70. Guang S., Bochner A.F., Burkhart K.B., Burton N., Pavelec D.M., Kennedy S. Small regulatory RNAs inhibit RNA polymerase II during the elongation phase of transcription. Nature 2010, 465(7301):1097-1101.
71. Guang S., Bochner A.F., Pavelec D.M., Burkhart K.B., Harding S., Lachowiec J., et al. An Argonaute transports siRNAs from the cytoplasm to the nucleus. Science 2008, 321(5888):537-541.
72. Gupta B.P., Hanna-Rose W., Sternberg P.W. Morphogenesis of the vulva and the vulval-uterine connection. WormBook 2012, 1-20. Nov 30.
73. Habig J.W., Aruscavage P.J., Bass B.L. In C. elegans, high levels of dsRNA allow RNAi in the absence of RDE-4. PLoS One 2008, 3(12):e4052.
74. Hall S.E., Chirn G.W., Lau N.C., Sengupta P. RNAi pathways contribute to developmental history-dependent phenotypic plasticity in C. elegans. RNA 2013, 19(3):306-319.
75. Hammell C.M. The microRNA-argonaute complex: A platform for mRNA modulation. RNA Biology 2008, 5(3):123-127.
76. Hammond S.M., Boettcher S., Caudy A.A., Kobayashi R., Hannon G.J. Argonaute2, a link between genetic and biochemical analyses of RNAi. Science 2001, 293(5532):1146-1150.
77. Han T., Manoharan A.P., Harkins T.T., Bouffard P., Fitzpatrick C., Chu D.S., et al. 26G endo-siRNAs regulate spermatogenic and zygotic gene expression in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America 2009, 106(44):18674-18679.
78. Han W., Sundaram P., Kenjale H., Grantham J., Timmons L. The Caenorhabditis elegans rsd-2 and rsd-6 genes are required for chromosome functions during exposure to unfavorable environments. Genetics 2008, 178(4):1875-1893.
79. Harrison M.M., Ceol C.J., Lu X., Horvitz H.R. Some C. elegans class B synthetic multivulva proteins encode a conserved LIN-35 Rb-containing complex distinct from a NuRD-like complex. Proceedings of the National Academy of Sciences of the United States of America 2006, 103(45):16782-16787.
80. Hayes G.D., Frand A.R., Ruvkun G. The mir-84 and let-7 paralogous microRNA genes of Caenorhabditis elegans direct the cessation of molting via the conserved nuclear hormone receptors NHR-23 and NHR-25. Development 2006, 133(23):4631-4641.
81. Hayes G.D., Riedel C.G., Ruvkun G. The Caenorhabditis elegans SOMI-1 zinc finger protein and SWI/SNF promote regulation of development by the mir-84 microRNA. Genes & Development 2011, 25(19):2079-2092.
82. Hinas A., Wright A.J., Hunter C.P. SID-5 is an endosome-associated protein required for efficient systemic RNAi in C. elegans. Current Biology 2012, 22(20):1938-1943.
83. Hobert O. Architecture of a microRNA-controlled gene regulatory network that diversifies neuronal cell fates. Cold Spring Harbor Symposia on Quantitative Biology 2006, 71:181-188.
84. Hundley H.A., Krauchuk A.A., Bass B.L. C. elegans and H. sapiens mRNAs with edited 3' UTRs are present on polysomes. RNA 2008, 14(10):2050-2060.
85. Hutvagner G., Simard M.J. Argonaute proteins: Key players in RNA silencing. Nature Reviews. Molecular Cell Biology 2008, 9(1):22-32.
86. Hutvagner G., Simard M.J., Mello C.C., Zamore P.D. Sequence-specific inhibition of small RNA function. PLoS Biology 2004, 2(4):E98.
87. Ibanez-Ventoso C., Driscoll M. MicroRNAs in C. elegans aging: molecular insurance for robustness?. Current Genomics 2009, 10(3):144-153.
88. Ibanez-Ventoso C., Yang M., Guo S., Robins H., Padgett R.W., Driscoll M. Modulated microRNA expression during adult lifespan in Caenorhabditis elegans. Aging Cell 2006, 5(3):235-246.
89. Ishizu H., Siomi H., Siomi M.C. Biology of PIWI-interacting RNAs: New insights into biogenesis and function inside and outside of germlines. Genes & Development 2012, 26(21):2361-2373.
90. Jannot G., Boisvert M.E., Banville I.H., Simard M.J. Two molecular features contribute to the Argonaute specificity for the microRNA and RNAi pathways in C. elegans. RNA 2008, 14(5):829-835.
91. Johnson S.M., Grosshans H., Shingara J., Byrom M., Jarvis R., Cheng A., et al. RAS is regulated by the let-7 microRNA family. Cell 2005, 120(5):635-647.
92. Johnston R.J., Hobert O. A microRNA controlling left/right neuronal asymmetry in Caenorhabditis elegans. Nature 2003, 426(6968):845-849.
93. Jose A.M., Garcia G.A., Hunter C.P. Two classes of silencing RNAs move between Caenorhabditis elegans tissues. Nature Structural and Molecular Biology 2011, 18(11):1184-1188.
94. Jose A.M., Kim Y.A., Leal-Ekman S., Hunter C.P. Conserved tyrosine kinase promotes the import of silencing RNA into Caenorhabditis elegans cells. Proceedings of the National Academy of Sciences of the United States of America 2012, 109(36):14520-14525.
95. Jose A.M., Smith J.J., Hunter C.P. Export of RNA silencing from C. elegans tissues does not require the RNA channel SID-1. Proceedings of the National Academy of Sciences of the United States of America 2009, 106(7):2283-2288.
96. Kai Z.S., Pasquinelli A.E. MicroRNA assassins: Factors that regulate the disappearance of miRNAs. Nature Structural and Molecular Biology 2010, 17(1):5-10.
97. Kamminga L.M., van Wolfswinkel J.C., Luteijn M.J., Kaaij L.J., Bagijn M.P., Sapetschnig A., et al. Differential impact of the HEN1 homolog HENN-1 on 21U and 26G RNAs in the germline of Caenorhabditis elegans. PLoS Genetics 2012, 8(7):e1002702.
98. Karp X., Hammell M., Ow M.C., Ambros V. Effect of life history on microRNA expression during C. elegans development. RNA 2011, 17(4):639-651.
99. Kato M., Chen X., Inukai S., Zhao H., Slack F.J. Age-associated changes in expression of small, noncoding RNAs, including microRNAs, in C. elegans. RNA 2011, 17(10):1804-1820.
100. Kato M., Paranjape T., Muller R.U., Nallur S., Gillespie E., Keane K., et al. The mir-34 microRNA is required for the DNA damage response in vivo in C. elegans and in vitro in human breast cancer cells. Oncogene 2009, 28(25):2419-2424.
101. Kato M., Slack F.J. Ageing and the small, non-coding RNA world. Ageing Research Reviews 2013, 12(1):429-435.
102. Kaufman E.J., Miska E.A. The microRNAs of Caenorhabditis elegans. Seminars in Cell & Developmental Biology 2010, 21(7):728-737.
103. Kemp B.J., Allman E., Immerman L., Mohnen M., Peters M.A., Nehrke K., et al. miR-786 regulation of a fatty-acid elongase contributes to rhythmic calcium-wave initiation in C. elegans. Current Biology 2012, 22(23):2213-2220.
104. Kennedy S., Wang D., Ruvkun G. A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans. Nature 2004, 427(6975):645-649.
105. Ketting R.F. MicroRNA biogenesis and function. An overview. Advances in Experimental Medicine and Biology 2010, 700:1-14.
106. Ketting R.F., Haverkamp T.H., van Luenen H.G., Plasterk R.H. Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell 1999, 99(2):133-141.
107. Ketting R.F., Plasterk R.H. A genetic link between co-suppression and RNA interference in C. elegans. Nature 2000, 404(6775):296-298.
108. Khurana J.S., Theurkauf W. piRNAs, transposon silencing, and Drosophila germline development. The Journal of Cell Biology 2010, 191(5):905-913.
109. Kim V.N., Han J., Siomi M.C. Biogenesis of small RNAs in animals. Nature Reviews. Molecular Cell Biology 2009, 10(2):126-139.
110. Knight S.W., Bass B.L. The role of RNA editing by ADARs in RNAi. Molecular Cell 2002, 10(4):809-817.
111. Krol J., Loedige I., Filipowicz W. The widespread regulation of microRNA biogenesis, function and decay. Nature Reviews. Genetics 2010, 11(9):597-610.
112. Kudron M., Niu W., Lu Z., Wang G., Gerstein M., Snyder M., et al. Tissue-specific direct targets of Caenorhabditis elegans Rb/E2F dictate distinct somatic and germline programs. Genome Biology 2013, 14(1):R5.
113. Lagos-Quintana M., Rauhut R., Lendeckel W., Tuschl T. Identification of novel genes coding for small expressed RNAs. Science 2001, 294(5543):853-858.
114. Lapierre L.R., Hansen M. Lessons from C. elegans: Signaling pathways for longevity. Trends in Endocrinology and Metabolism 2012, 23(12):637-644.
115. Lau N.C., Lim L.P., Weinstein E.G., Bartel D.P. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science 2001, 294(5543):858-862.
116. Lee R.C., Ambros V. An extensive class of small RNAs in Caenorhabditis elegans. Science 2001, 294(5543):862-864.
117. Lee R.C., Feinbaum R.L., Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 1993, 75(5):843-854.
118. Lee H.C., Gu W., Shirayama M., Youngman E., Conte D., Mello C.C. C. elegans piRNAs mediate the genome-wide surveillance of germline transcripts. Cell 2012, 150(1):78-87.
119. Lee R.C., Hammell C.M., Ambros V. Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans. RNA 2006, 12(4):589-597.
120. Lehner B., Calixto A., Crombie C., Tischler J., Fortunato A., Chalfie M., et al. Loss of LIN-35, the Caenorhabditis elegans ortholog of the tumor suppressor p105Rb, results in enhanced RNA interference. Genome Biology 2006, 7(1):R4.
121. Lehrbach N.J., Castro C., Murfitt K.J., Abreu-Goodger C., Griffin J.L., Miska E.A. Post-developmental microRNA expression is required for normal physiology, and regulates aging in parallel to insulin/IGF-1 signaling in C. elegans. RNA 2012, 18(12):2220-2235.
122. Lehrbach N.J., Miska E.A. Regulation of pre-miRNA processing. Advances in Experimental Medicine and Biology 2010, 700:67-75.
123. Li J., Greenwald I. LIN-14 inhibition of LIN-12 contributes to precision and timing of C. elegans vulval fate patterning. Current Biology 2010, 20(20):1875-1879.
124. Li M., Jones-Rhoades M.W., Lau N.C., Bartel D.P., Rougvie A.E. Regulatory mutations of mir-48, a C. elegans let-7 family MicroRNA, cause developmental timing defects. Developmental Cell 2005, 9(3):415-422.
125. Lim L.P., Lau N.C., Weinstein E.G., Abdelhakim A., Yekta S., Rhoades M.W., et al. The microRNAs of Caenorhabditis elegans. Genes & Development 2003, 17(8):991-1008.
126. Lu R., Maduro M., Li F., Li H.W., Broitman-Maduro G., Li W.X., et al. Animal virus replication and RNAi-mediated antiviral silencing in Caenorhabditis elegans. Nature 2005, 436(7053):1040-1043.
127. Luteijn M.J., van Bergeijk P., Kaaij L.J., Almeida M.V., Roovers E.F., Berezikov E., et al. Extremely stable Piwi-induced gene silencing in Caenorhabditis elegans. EMBO Journal 2012, 31(16):3422-3430.
128. Maine E.M., Hauth J., Ratliff T., Vought V.E., She X., Kelly W.G. EGO-1, a putative RNA-dependent RNA polymerase, is required for heterochromatin assembly on unpaired dna during C. elegans meiosis. Current Biology 2005, 15(21):1972-1978.
129. Maniar J.M., Fire A.Z. EGO-1, a C. elegans RdRP, modulates gene expression via production of mRNA-templated short antisense RNAs. Current Biology 2011, 21(6):449-459.
130. Mansisidor A.R., Cecere G., Hoersch S., Jensen M.B., Kawli T., Kennedy L.M., et al. A conserved PHD finger protein and endogenous RNAi modulate insulin signaling in Caenorhabditis elegans. PLoS Genetics 2011, 7(9):e1002299.
131. Margulies M., Egholm M., Altman W.E., Attiya S., Bader J.S., Bemben L.A., et al. Genome sequencing in microfabricated high-density picolitre reactors. Nature 2005, 437(7057):376-380.
132. Marzluff W.F., Wagner E.J., Duronio R.J. Metabolism and regulation of canonical histone mRNAs: Life without a poly(A) tail. Nature Reviews. Genetics 2008, 9(11):843-854.
133. Massirer K.B., Perez S.G., Mondol V., Pasquinelli A.E. The miR-35-41 family of microRNAs regulates RNAi sensitivity in Caenorhabditis elegans. PLoS Genetics 2012, 8(3):e1002536.
134. McEwan D.L., Weisman A.S., Hunter C.P. Uptake of extracellular double-stranded RNA by SID-2. Molecular Cell 2012, 47(5):746-754.
135. McManus M.T., Petersen C.P., Haines B.B., Chen J., Sharp P.A. Gene silencing using micro-RNA designed hairpins. RNA 2002, 8(6):842-850.
136. McManus M.T., Sharp P.A. Gene silencing in mammals by small interfering RNAs. Nature Reviews. Genetics 2002, 3(10):737-747.
137. Miska E.A., Alvarez-Saavedra E., Abbott A.L., Lau N.C., Hellman A.B., McGonagle S.M., et al. Most Caenorhabditis elegans microRNAs are individually not essential for development or viability. PLoS Genetics 2007, 3(12):e215.
138. Mondol V., Pasquinelli A.E. Let's make it happen: The role of let-7 microRNA in development. Current Topics in Developmental Biology 2012, 99:1-30.
139. Montgomery T.A., Rim Y.S., Zhang C., Dowen R.H., Phillips C.M., Fischer S.E., et al. PIWI associated siRNAs and piRNAs specifically require the Caenorhabditis elegans HEN1 ortholog henn-1. PLoS Genetics 2012, 8(4):e1002616.
140. Nakamura M., Ando R., Nakazawa T., Yudazono T., Tsutsumi N., Hatanaka N., et al. Dicer-related drh-3 gene functions in germ-line development by maintenance of chromosomal integrity in Caenorhabditis elegans. Genes to Cells 2007, 12(9):997-1010.
141. Nuez I., Felix M.A. Evolution of susceptibility to ingested double-stranded RNAs in Caenorhabditis nematodes. PLoS One 2012, 7(1):e29811.
142. Obsil T., Obsilova V. Structural basis for DNA recognition by FOXO proteins. Biochimica et Biophysica Acta 2011, 1813(11):1946-1953.
143. Okamura K., Lai E.C. Endogenous small interfering RNAs in animals. Nature Reviews. Molecular Cell Biology 2008, 9(9):673-678.
144. Olsson-Carter K., Slack F.J. A developmental timing switch promotes axon outgrowth independent of known guidance receptors. PLoS Genetics 2010, 6(8):e1001054.
145. Ouellet J., Roy R. The lin-35/Rb and RNAi pathways cooperate to regulate a key cell cycle transition in C. elegans. BMC Developmental Biology 2007, 7:38.
146. Pak J., Fire A. Distinct populations of primary and secondary effectors during RNAi in C. elegans. Science 2007, 315(5809):241-244.
147. Pak J., Maniar J.M., Mello C.C., Fire A. Protection from feed-forward amplification in an amplified RNAi mechanism. Cell 2012, 151(4):885-899.
148. Parker G.S., Eckert D.M., Bass B.L. RDE-4 preferentially binds long dsRNA and its dimerization is necessary for cleavage of dsRNA to siRNA. RNA 2006, 12(5):807-818.
149. Parker G.S., Maity T.S., Bass B.L. dsRNA binding properties of RDE-4 and TRBP reflect their distinct roles in RNAi. Journal of Molecular Biology 2008, 384(4):967-979.
150. Parrish S., Fire A. Distinct roles for RDE-1 and RDE-4 during RNA interference in Caenorhabditis elegans. RNA 2001, 7(10):1397-1402.
151. Pasquinelli A.E. MicroRNAs and their targets: Recognition, regulation and an emerging reciprocal relationship. Nature Reviews. Genetics 2012, 13(4):271-282.
152. Pasquinelli A.E., Reinhart B.J., Slack F., Martindale M.Q., Kuroda M.I., Maller B., et al. Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature 2000, 408(6808):86-89.
153. Pavelec D.M., Lachowiec J., Duchaine T.F., Smith H.E., Kennedy S. Requirement for the ERI/DICER complex in endogenous RNA interference and sperm development in Caenorhabditis elegans. Genetics 2009, 183(4):1283-1295.
154. Phillips C.M., Montgomery T.A., Breen P.C., Ruvkun G. MUT-16 promotes formation of perinuclear mutator foci required for RNA silencing in the C. elegans germline. Genes & Development 2012, 26(13):1433-1444.
155. Rechtsteiner A., Ercan S., Takasaki T., Phippen T.M., Egelhofer T.A., Wang W., et al. The histone H3K36 methyltransferase MES-4 acts epigenetically to transmit the memory of germline gene expression to progeny. PLoS Genetics 2010, 6(9):e1001091.
156. Reinhart B.J., Slack F.J., Basson M., Pasquinelli A.E., Bettinger J.C., Rougvie A.E., et al. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 2000, 403(6772):901-906.
157. Resnick T.D., McCulloch K.A., Rougvie A.E. miRNAs give worms the time of their lives: Small RNAs and temporal control in Caenorhabditis elegans. Developmental Dynamics 2010, 239(5):1477-1489.
158. Rocheleau C.E., Cullison K., Huang K., Bernstein Y., Spilker A.C., Sundaram M.V. The Caenorhabditis elegans ekl (enhancer of ksr-1 lethality) genes include putative components of a germline small RNA pathway. Genetics 2008, 178(3):1431-1443.
159. Rougvie A.E. Intrinsic and extrinsic regulators of developmental timing: From miRNAs to nutritional cues. Development 2005, 132(17):3787-3798.
160. Ruby J.G., Jan C., Player C., Axtell M.J., Lee W., Nusbaum C., et al. Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans. Cell 2006, 127(6):1193-1207.
161. Ruvkun G. The perfect storm of tiny RNAs. Nature Medicine 2008, 14(10):1041-1045.
162. Sabin L.R., Delas M.J., Hannon G.J. Dogma derailed: The many influences of RNA on the genome. Molecular Cell 2013, 49(5):783-794.
163. Schmitz C., Wacker I., Hutter H. The Fat-like cadherin CDH-4 controls axon fasciculation, cell migration and hypodermis and pharynx development in Caenorhabditis elegans. Developmental Biology 2008, 316(2):249-259.
164. Schott D.H., Cureton D.K., Whelan S.P., Hunter C.P. An antiviral role for the RNA interference machinery in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America 2005, 102(51):18420-18424.
165. Shaw W.R., Armisen J., Lehrbach N.J., Miska E.A. The conserved miR-51 microRNA family is redundantly required for embryonic development and pharynx attachment in Caenorhabditis elegans. Genetics 2010, 185(3):897-905.
166. She X., Xu X., Fedotov A., Kelly W.G., Maine E.M. Regulation of heterochromatin assembly on unpaired chromosomes during Caenorhabditis elegans meiosis by components of a small RNA-mediated pathway. PLoS Genetics 2009, 5(8):e1000624.
167. Shen Y., Wollam J., Magner D., Karalay O., Antebi A. A steroid receptor-microRNA switch regulates life span in response to signals from the gonad. Science 2012, 338(6113):1472-1476.
168. Shi Z., Montgomery T.A., Qi Y., Ruvkun G. High-throughput sequencing reveals extraordinary fluidity of miRNA, piRNA, and siRNA pathways in nematodes. Genome Research 2013, 23(3):497-508.
169. Shih J.D., Fitzgerald M.C., Sutherlin M., Hunter C.P. The SID-1 double-stranded RNA transporter is not selective for dsRNA length. RNA 2009, 15(3):384-390.
170. Shih J.D., Hunter C.P. SID-1 is a dsRNA-selective dsRNA-gated channel. RNA 2011, 17(6):1057-1065.
171. Shirayama M., Seth M., Lee H.C., Gu W., Ishidate T., Conte D., et al. piRNAs initiate an epigenetic memory of nonself RNA in the C. elegans germline. Cell 2012, 150(1):65-77.
172. Sijen T., Fleenor J., Simmer F., Thijssen K.L., Parrish S., Timmons L., et al. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell 2001, 107(4):465-476.
173. Sijen T., Plasterk R.H. Transposon silencing in the Caenorhabditis elegans germ line by natural RNAi. Nature 2003, 426(6964):310-314.
174. Sijen T., Steiner F.A., Thijssen K.L., Plasterk R.H. Secondary siRNAs result from unprimed RNA synthesis and form a distinct class. Science 2007, 315(5809):244-247.
175. Simmer F., Tijsterman M., Parrish S., Koushika S.P., Nonet M.L., Fire A., et al. Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Current Biology 2002, 12(15):1317-1319.
176. Simon D.J., Madison J.M., Conery A.L., Thompson-Peer K.L., Soskis M., Ruvkun G.B., et al. The microRNA miR-1 regulates a MEF-2-dependent retrograde signal at neuromuscular junctions. Cell 2008, 133(5):903-915.
177. Smardon A., Spoerke J.M., Stacey S.C., Klein M.E., Mackin N., Maine E.M. EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans. Current Biology 2000, 10(4):169-178.
178. Smith-Vikos T., Slack F.J. MicroRNAs and their roles in aging. Journal of Cell Science 2012, 125(Pt. 1):7-17.
179. Sokol N.S. Small temporal RNAs in animal development. Current Opinion in Genetics and Development 2012, 22(4):368-373.
180. Sokol N.S., Ambros V. Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles during larval growth. Genes & Development 2005, 19(19):2343-2354.
181. Spike C.A., Bader J., Reinke V., Strome S. DEPS-1 promotes P-granule assembly and RNA interference in C. elegans germ cells. Development 2008, 135(5):983-993.
182. Steiner F.A., Hoogstrate S.W., Okihara K.L., Thijssen K.L., Ketting R.F., Plasterk R.H., et al. Structural features of small RNA precursors determine Argonaute loading in Caenorhabditis elegans. Nature Structural and Molecular Biology 2007, 14(10):927-933.
183. Steiner F.A., Okihara K.L., Hoogstrate S.W., Sijen T., Ketting R.F. RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans. Nature Structural and Molecular Biology 2009, 16(2):207-211.
184. Sundaram P., Echalier B., Han W., Hull D., Timmons L. ATP-binding cassette transporters are required for efficient RNA interference in Caenorhabditis elegans. Molecular Biology of the Cell 2006, 17(8):3678-3688.
185. Sundaram P., Han W., Cohen N., Echalier B., Albin J., Timmons L. Caenorhabditis elegans ABCRNAi transporters interact genetically with rde-2 and mut-7. Genetics 2008, 178(2):801-814.
186. Tabach Y., Billi A.C., Hayes G.D., Newman M.A., Zuk O., Gabel H., et al. Identification of small RNA pathway genes using patterns of phylogenetic conservation and divergence. Nature 2013, 493(7434):694-698.
187. Tabara H., Sarkissian M., Kelly W.G., Fleenor J., Grishok A., Timmons L., et al. The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 1999, 99(2):123-132.
188. Tabara H., Yigit E., Siomi H., Mello C.C. The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegans. Cell 2002, 109(7):861-871.
189. Thivierge C., Makil N., Flamand M., Vasale J.J., Mello C.C., Wohlschlegel J., et al. Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi. Nature Structural and Molecular Biology 2012, 19(1):90-97.
190. Tijsterman M., Ketting R.F., Okihara K.L., Sijen T., Plasterk R.H. RNA helicase MUT-14-dependent gene silencing triggered in C. elegans by short antisense RNAs. Science 2002, 295(5555):694-697.
191. Tijsterman M., May R.C., Simmer F., Okihara K.L., Plasterk R.H. Genes required for systemic RNA interference in Caenorhabditis elegans. Current Biology 2004, 14(2):111-116.
192. Tijsterman M., Okihara K.L., Thijssen K., Plasterk R.H. PPW-1, a PAZ/PIWI protein required for efficient germline RNAi, is defective in a natural isolate of C. elegans. Current Biology 2002, 12(17):1535-1540.
193. Timmons L., Fire A. Specific interference by ingested dsRNA. Nature 1998, 395(6705):854.
194. Tonkin L.A., Saccomanno L., Morse D.P., Brodigan T., Krause M., Bass B.L. RNA editing by ADARs is important for normal behavior in Caenorhabditis elegans. EMBO Journal 2002, 21(22):6025-6035.
195. Tops B.B., Tabara H., Sijen T., Simmer F., Mello C.C., Plasterk R.H., et al. RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans. Nucleic Acids Research 2005, 33(1):347-355.
196. Turner M.J., Slack F.J. Transcriptional control of microRNA expression in C. elegans: Promoting better understanding. RNA Biology 2009, 6(1):49-53.
197. Unhavaithaya Y., Shin T.H., Miliaras N., Lee J., Oyama T., Mello C.C. MEP-1 and a homolog of the NURD complex component Mi-2 act together to maintain germline-soma distinctions in C. elegans. Cell 2002, 111(7):991-1002.
198. Updike D.L., Strome S. A genomewide RNAi screen for genes that affect the stability, distribution and function of P granules in Caenorhabditis elegans. Genetics 2009, 183(4):1397-1419.
199. van Wolfswinkel J.C., Claycomb J.M., Batista P.J., Mello C.C., Berezikov E., Ketting R.F. CDE-1 affects chromosome segregation through uridylation of CSR-1-bound siRNAs. Cell 2009, 139(1):135-148.
200. Vasale J.J., Gu W., Thivierge C., Batista P.J., Claycomb J.M., Youngman E.M., et al. Sequential rounds of RNA-dependent RNA transcription drive endogenous small-RNA biogenesis in the ERGO-1/Argonaute pathway. Proceedings of the National Academy of Sciences of the United States of America 2010, 107(8):3582-3587.
201. Vasquez-Rifo A., Jannot G., Armisen J., Labouesse M., Bukhari S.I., Rondeau E.L., et al. Developmental characterization of the microRNA-specific C. elegans Argonautes alg-1 and alg-2. PLoS One 2012, 7(3):e33750.
202. Vastenhouw N.L., Brunschwig K., Okihara K.L., Muller F., Tijsterman M., Plasterk R.H. Gene expression: Long-term gene silencing by RNAi. Nature 2006, 442(7105):882.
203. Vastenhouw N.L., Fischer S.E., Robert V.J., Thijssen K.L., Fraser A.G., Kamath R.S., et al. A genome-wide screen identifies 27 genes involved in transposon silencing in C. elegans. Current Biology 2003, 13(15):1311-1316.
204. Vella M.C., Slack F.J. C. elegans microRNAs. WormBook 2005, 1-9. Sep 21.
205. Vought V.E., Ohmachi M., Lee M.H., Maine E.M. EGO-1, a putative RNA-directed RNA polymerase, promotes germline proliferation in parallel with GLP-1/notch signaling and regulates the spatial organization of nuclear pore complexes and germline P granules in Caenorhabditis elegans. Genetics 2005, 170(3):1121-1132.
206. Wang D., Kennedy S., Conte D., Kim J.K., Gabel H.W., Kamath R.S., et al. Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants. Nature 2005, 436(7050):593-597.
207. Wang G., Reinke V. A C. elegans Piwi, PRG-1, regulates 21U-RNAs during spermatogenesis. Current Biology 2008, 18(12):861-867.
208. Warf M.B., Shepherd B.A., Johnson W.E., Bass B.L. Effects of ADARs on small RNA processing pathways in C. elegans. Genome Research 2012, 22(8):1488-1498.
209. Welker N.C., Habig J.W., Bass B.L. Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes. RNA 2007, 13(7):1090-1102.
210. Welker N.C., Maity T.S., Ye X., Aruscavage P.J., Krauchuk A.A., Liu Q., et al. Dicer's helicase domain discriminates dsRNA termini to promote an altered reaction mode. Molecular Cell 2011, 41(5):589-599.
211. Welker N.C., Pavelec D.M., Nix D.A., Duchaine T.F., Kennedy S., Bass B.L. Dicer's helicase domain is required for accumulation of some, but not all, C. elegans endogenous siRNAs. RNA 2010, 16(5):893-903.
212. Whangbo J.S., Hunter C.P. Environmental RNA interference. Trends in Genetics 2008, 24(6):297-305.
213. Wightman B., Ha I., Ruvkun G. Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 1993, 75(5):855-862.
214. Wilkins C., Dishongh R., Moore S.C., Whitt M.A., Chow M., Machaca K. RNA interference is an antiviral defence mechanism in Caenorhabditis elegans. Nature 2005, 436(7053):1044-1047.
215. Winston W.M., Molodowitch C., Hunter C.P. Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1. Science 2002, 295(5564):2456-2459.
216. Winston W.M., Sutherlin M., Wright A.J., Feinberg E.H., Hunter C.P. Caenorhabditis elegans SID-2 is required for environmental RNA interference. Proceedings of the National Academy of Sciences of the United States of America 2007, 104(25):10565-10570.
217. Wu D., Lamm A.T., Fire A.Z. Competition between ADAR and RNAi pathways for an extensive class of RNA targets. Nature Structural and Molecular Biology 2011, 18(10):1094-1101.
218. Wu X., Shi Z., Cui M., Han M., Ruvkun G. Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes. PLoS Genetics 2012, 8(3):e1002542.
219. Wu E., Thivierge C., Flamand M., Mathonnet G., Vashisht A.A., Wohlschlegel J., et al. Pervasive and cooperative deadenylation of 3'UTRs by embryonic microRNA families. Molecular Cell 2010, 40(4):558-570.
220. Yang J., Chen D., He Y., Melendez A., Feng Z., Hong Q., et al. MiR-34 modulates Caenorhabditis elegans lifespan via repressing the autophagy gene atg9. Age (Dordrecht, Netherlands) 2013, 35(1):11-22.
221. Yang H., Zhang Y., Vallandingham J., Li H., Florens L., Mak H.Y. The RDE-10/RDE-11 complex triggers RNAi-induced mRNA degradation by association with target mRNA in C. elegans. Genes & Development 2012, 26(8):846-856.
222. Yigit E., Batista P.J., Bei Y., Pang K.M., Chen C.C., Tolia N.H., et al. Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Cell 2006, 127(4):747-757.
223. Yoo A.S., Greenwald I. LIN-12/Notch activation leads to microRNA-mediated down-regulation of Vav in C. elegans. Science 2005, 310(5752):1330-1333.
224. Zhang C., Montgomery T.A., Fischer S.E., Garcia S.M., Riedel C.G., Fahlgren N., et al. The Caenorhabditis elegans RDE-10/RDE-11 complex regulates RNAi by promoting secondary siRNA amplification. Current Biology 2012, 22(10):881-890.
225. Zhang C., Montgomery T.A., Gabel H.W., Fischer S.E., Phillips C.M., Fahlgren N., et al. mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans. Proceedings of the National Academy of Sciences of the United States of America 2011, 108(4):1201-1208.
226. Zhao Z., Boyle T.J., Liu Z., Murray J.I., Wood W.B., Waterston R.H. A negative regulatory loop between microRNA and Hox gene controls posterior identities in Caenorhabditis elegans. PLoS Genetics 2010, 6(9):e1001089.
227. Zhao Y., Ransom J.F., Li A., Vedantham V., von Drehle M., Muth A.N., et al. Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2. Cell 2007, 129(2):303-317.
228. Zhuang J.J., Hunter C.P. RNA interference in Caenorhabditis elegans: Uptake, mechanism, and regulation. Parasitology 2012, 139(5):560-573.
229. Zisoulis D.G., Kai Z.S., Chang R.K., Pasquinelli A.E. Autoregulation of microRNA biogenesis by let-7 and Argonaute. Nature 2012, 486(7404):541-544.