메뉴 건너뛰기




Volumn 287, Issue 5462, 2000, Pages 2494-2497

Genetic requirements for inheritance of RNAi in C. elegans

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

EID: 0034737747     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.287.5462.2494     Document Type: Article
Times cited : (385)

References (31)
  • 2
    • 0032076565 scopus 로고    scopus 로고
    • M. A. Surani, Cell 93, 309 (1998).
    • (1998) Cell , vol.93 , pp. 309
    • Surani, M.A.1
  • 6
    • 0032545933 scopus 로고    scopus 로고
    • A. Fire et al., Nature 391, 806 (1998).
    • (1998) Nature , vol.391 , pp. 806
    • Fire, A.1
  • 15
    • 0032717319 scopus 로고    scopus 로고
    • H. Tabara et al., Cell 99, 123 (1999).
    • (1999) Cell , vol.99 , pp. 123
    • Tabara, H.1
  • 16
    • 0342769847 scopus 로고    scopus 로고
    • note
    • 2 when injected into the intestine of hermaphrodites.
  • 18
    • 0343640104 scopus 로고    scopus 로고
    • note
    • 1 animals scored for each RNA tested).
  • 22
    • 0342769845 scopus 로고    scopus 로고
    • in preparation
    • Embryonic lethal phenotype resulting from inactivation of sgg-1 in the injected mother will be described elsewhere (Y. Bei, in preparation).
    • Bei, Y.1
  • 23
    • 0342769844 scopus 로고    scopus 로고
    • note
    • 1 cross-progeny. This type of inheritance occurs only in hermaphrodites and may reflect a passive transfer of the injected material into the maternal germ line. The activities of rde-1 and rde-4 genes are necessary in the ensuing generation for the initiation of interference in response to this inherited material.
  • 24
    • 0342334945 scopus 로고    scopus 로고
    • note
    • 2 progeny were examined for affected embryos. No pos-1 affected embryos were observed in any generation.
  • 25
    • 0342769843 scopus 로고    scopus 로고
    • note
    • Thirty-five rde-1 homozygous animals generated through crosses shown in Fig. 3A were tested by feeding bacteria expressing pos-1 dsRNA and 21 similar animals were tested by direct injections of pos-1 dsRNA; all animals tested were resistant to pos-1 (RNAi).
  • 26
    • 0032739015 scopus 로고    scopus 로고
    • R. Ketting et al., Cell 99, 133 (1999).
    • (1999) Cell , vol.99 , pp. 133
    • Ketting, R.1
  • 27
    • 0032955004 scopus 로고    scopus 로고
    • A. Schmidt et al., Genetics 151, 749 (1999).
    • (1999) Genetics , vol.151 , pp. 749
    • Schmidt, A.1
  • 29
    • 0030829387 scopus 로고    scopus 로고
    • C. E. Rocheleau et al., Cell 90, 707 (1997).
    • (1997) Cell , vol.90 , pp. 707
    • Rocheleau, C.E.1
  • 30
    • 0342334944 scopus 로고    scopus 로고
    • note
    • The alleles of RNAi-deficient mutants used in this study were as follows: rde-1(ne300) unc-42, rde-1(ne219), rde-2(ne221), rde-4(ne299), and mut-7(pk204).
  • 31
    • 0343640102 scopus 로고    scopus 로고
    • note
    • We thank T. H. Shin, M. Soto, A. Fire, and members of our laboratory for many discussions; Y. Bei and T. Shin for help in scoring RNAi mutant embryos; G. Seydoux for the pes-10::gfp strain; and the anonymous reviewer for helpful suggestions. Some strains were obtained from the C. elegans Genetic Stock Center, which is funded by a grant from the NIH National Center for Research Support. Supported in part by a Pew scholarship to C.C.M. and by NIH grant GM58800.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.