Secondary siRNAs result from unprimed RNA synthesis and form a distinct class

Science

Volumn 315, Issue 5809, 2007, Pages 244-247

  Sijen, Titia ^a   Steiner, Florian A ^a   Thijssen, Karen L ^a   Plasterk, Ronald H A ^a  

^a HUBRECHT INSTITUTE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN; PROTEIN ARGONAUTE; RNA DIRECTED RNA POLYMERASE; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ENZYME; NEMATODE; NUCLEIC ACID; PROTEIN; RNA;

ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; GENE SILENCING; NONHUMAN; PRIORITY JOURNAL; RNA CLEAVAGE; RNA INTERFERENCE; RNA SYNTHESIS;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BASE PAIRING; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; CLONING, MOLECULAR; PHOSPHATES; RIBONUCLEASE III; RNA INTERFERENCE; RNA REPLICASE; RNA, ANTISENSE; RNA, COMPLEMENTARY; RNA, HELMINTH; RNA, MESSENGER; RNA, SMALL INTERFERING;

CAENORHABDITIS ELEGANS;

EID: 33846524902     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1136699     Document Type: Article

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24. We thank G. Hannon and F. Rivas for help with the capture assays; R. Ketting for sharing unpublished data on the identity of mut-15 and for the 5′ linker; W. Verheul, J. van de Belt, and E. Berezikov for help with sequence analyses; C. Mello for providing strains; M. Darding for experimental help; B. Ason and M. Joosten for critically reading the manuscript; and A. Fire for the suggestion to use 9:1 gels. The work was supported by a Vidi fellowship from the Dutch Scientific Organization (NWO) to T.S. and a European Union grant (HPRN-CT-2002-00257) and Netherlands Genomic Initiative grant (050-72-415) to F.A.S.