Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut +

Journal of Biotechnology

Volumn 157, Issue 1, 2012, Pages 180-188

  Paulová, Leona ^a   Hyka, Petr ^a,b   Branská, Barbora ^a   Melzoch, Karel ^a   Kovar, Karin ^b  

^a INSTITUTE OF CHEMICAL TECHNOLOGY   (Czech Republic)

^b ZURICH UNIVERSITY OF APPLIED SCIENCES   (Switzerland)

Author keywords

Continuous culture; Glucose repression; Mixed substrate; Pichia pastoris; Porcine trypsinogen production

Indexed keywords

A-CARBON; BATCH CULTURE; BIOMASS GROWTH; CARBON SUBSTRATES; CELL VIABILITY; CONTINUOUS CULTURE; DILUTION RATE; EXTRACELLULAR; GLUCOSE REPRESSION; IN-PROCESS; MAXIMUM PRODUCTIVITY; METHANOL UTILIZATION; P. PASTORIS; PICHIA PASTORIS; PROCESS PRODUCTIVITY; RECOMBINANT PROTEIN PRODUCTIONS;

BATCH CELL CULTURE; CARBON; GLUCOSE; METHANOL; PRODUCTIVITY; PROTEINS; YEAST;

SUBSTRATES;

CARBON; GLUCOSE; METHANOL; RECOMBINANT PROTEIN; TRYPSINOGEN;

ARTICLE; BACTERIUM CULTURE; BIOMASS PRODUCTION; CARBON SOURCE; CELL VIABILITY; CONTINUOUS CULTURE; CONTROLLED STUDY; DILUTION; ENERGY; METABOLISM; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL;

ANIMALS; BIOMASS; BIOREACTORS; CELL SURVIVAL; CULTURE MEDIA; GLUCOSE; KINETICS; METHANOL; PICHIA; RECOMBINANT PROTEINS; SWINE; TRYPSINOGEN;

PICHIA PASTORIS; SUS;

EID: 84855211850     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.10.010     Document Type: Article

References (73)

1. Abad S., Nahalka J., Bergler G., Arnold S.A., Speight R., Fotheringham I., Nidetzky B., Glieder A. Stepwise engineering of a Pichia pastoris d-amino acid oxidase whole cell catalyst. Microb. Cell Fact. 2010, 9:24.
2. Blank L.M., Kuepfer L. Metabolic flux distributions: genetic information, computational predictions, and experimental validation. Appl. Microbiol. Biotechnol. 2010, 86:1243-1255.
3. Brierley, R.A., Siegel, R.S., Bussineau, C.M., Craig, W.S., Holtz, G.C., Davis, G.R., Buckholz, R.G., Thill, G.P., Wondrack, L.M., Digan, M.E., Harpold, M.M., Lair, S.V., Ellis, S.B., Williams, M.E., 1990. Mixed feed recombinant yeast fermentation. US Patent, WO9003431.
4. Celik E., Calik P., Oliver S.G. Metabolic flux analysis for recombinant protein production by Pichia pastoris using dual carbon sources: effects of methanol feeding rate. Biotechnol. Bioeng. 2010, 105:317-329.
5. Cereghino J.L., Cregg J.M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 2000, 24:45-66.
6. Cos O., Ramon R., Montesinos J.L., Vallero F. A simple model-based control for Pichia pastoris allows a more efficient heterologous protein production bioprocess. Biotechnol. Bioeng. 2006, 95:145-154.
7. Cregg J.M., Cereghino J.L., Shi J., Higgins D.R. Recombinant protein expression in Pichia pastoris. Mol. Biotechnol. 2000, 16:23-52.
8. Cregg J.M., Tschopp J.F., Stillman C., Siegel R., Akong M., Craig W.S., Buckholz R.G., Madden K.R., Kellaris P.A., Davis G.R., Smiley B.L., Cruze J., Torregrossa R., Velicelebi G., Thill G.P. High-level expression and efficient assembly of hepatitis-B surface-antigen in the methylotrophic yeast, Pichia pastoris. Bio-Technology Biotechnol. 1987, 5:479-485.
9. Cunha A.E., Clemente J.J., Gomes R., Pinto F., Thomaz M., Miranda S., Pinto R., Moosmayer D., Donner P., Carrondo M.J.T. Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol. Bioeng. 2004, 86:458-467.
10. Curvers S., Linneman J., Klauser T., Wandrey C., Takors R. Recombinant protein production with Pichia pastoris in continuous fermentation - kinetic analysis of growth and product formation. Chem. Ing. Tech. 2001, 73:1615-1621.
11. d'Anjou M.C., Daugulis A.J. A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol. Bioeng. 2001, 72:1-11.
12. De Schutter K., Lin Y.C., Tiels P., Van Hecke A., Glinka S., Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N. Genome sequence of the recombinant protein production host Pichia pastoris. Nat. Biotechnol. 2009, 27:561-566.
13. Egli T., Bosshard C., Hamer G. Simultaneous utilization of methanol-glucose mixtures by Hansenula polymorpha in chemostat: influence of dilution rate and mixture composition on utilization pattern. Biotechnol. Bioeng. 1986, 28:1735-1741.
14. Egli T., Harder W. Growth of methylotrophs on mixed substrates. Microbial growth on C1 compound. Am. Soc. Microbiol. 1984, 330-337.
15. Egli T., Kappeli O., Fiechter A. Mixed substrate growth of methylotrophic yeasts in chemostat culture - influence of the dilution rate on the utilization of a mixture of glucose and methanol. Arch. Microbiol. 1982, 131:8-13.
16. Egli T., Kappeli O., Fiechter A. Regulatory flexibility of methylotrophic yeasts in chemostat cultures - simultaneous assimilation of glucose and methanol at a fixed dilution rate. Arch. Microbiol. 1982, 131:1-7.
17. Egli T., Lindley N.D., Quayle J.R. Regulation of enzyme synthesis and variation of residual methanol concentration during carbon-limited growth of Kloeckera sp. 2201 on mixtures of methanol and glucose. J. Gen. Microbiol. 1983, 129:1269-1281.
18. Egli T., Mason C.A. Mixed substrates and mixed cultures. Biology of Methylotrophs 1991, Butterworth-Heinemann, Boston. I. Goldberg, J.S. Rokem (Eds.).
19. Files D., Ogawa M., Scaman C.H., Baldwin S.A. A Pichia pastoris fermentation process for producing high-levels of recombinant human cystatin-C. Enzyme Microb. Technol. 2001, 29:335-340.
20. Gasser B., Maurer M., Rautio J., Sauer M., Bhattacharyya A., Saloheimo M., Penttila M., Mattanovich D. Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions. BMC Genomics 2007, 8:179.
21. Giuseppin M.L.F. Mathematical modeling of growth and production of alcohol oxidase by Hansenula polymorpha grown on methanol-glucose mixtures. Biotechnol. Bioeng. 1989, 33:524-535.
22. Giuseppin M.L.F., Vaneijk H.M.J., Bes B.C.M. Molecular regulation of methanol oxidase activity in continuous cultures of Hansenula polymorpha. Biotechnol. Bioeng. 1988, 32:577-583.
23. Graf A., Dragosits M., Gasser B., Mattanovich D. Yeast systems biotechnology for the production of heterologous proteins. FEMS Yeast Res. 2009, 9:335-348.
24. Graf A., Gasser B., Dragosits M., Sauer M., Leparc G.G., Tuchler T., Kreil D.P., Mattanovich D. Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays. BMC Genomics 2008, 9:390.
25. Hang H.F., Chen W., Guo M.J., Chu J., Zhuang Y.P., Zhang S.L. A simple unstructured model-based control for efficient expression of recombinant porcine insulin precursor by Pichia pastoris. Korean J. Chem. Eng. 2008, 25:1065-1069.
26. Hanquier J., Sorlet Y., Desplancq D., Baroche L., Ebtinger M., Lefevre J.F., Pattus F., Hershberger C.L., Vertes A.A. A single mutation in the activation site of bovine trypsinogen enhances its accumulation in the fermentation broth of the yeast Pichia pastoris. Appl. Environ. Microbiol. 2003, 69:1108-1113.
27. Hanquier, J.M., Hershberger, C.L., Desplancq, D., Larson, J.L., 2000. Production of soluble recombinant trypsinogen analogs. US, WO00/17332.
28. Hartner F.S., Glieder A. Regulation of methanol utilisation pathway genes in yeasts. Microb. Cell Fact. 2006, 5:39.
29. Hartner F.S., Ruth C., Langenegger D., Johnson S.N., Hyka P., Lin-Cereghino G.P., Lin-Cereghino J., Kovar K., Cregg J.M., Glieder A. Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res. 2008, 36:e76.
30. Hellwig S., Emde F., Raven N.P., Henke M., van Der Logt P., Fischer R. Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol. Bioeng. 2001, 74:344-352.
31. Hewitt C.J., Nebe-von Caron G., Nienow A.W., McFarlane C.M. The use of multi-parameter flow cytometry to compare the physiological response of Escherichia coli W3110 to glucose limitation during batch, fed-batch and continuous culture cultivations. J. Biotechnol. 1999, 75:251-264.
32. Heyland J., Fu J., Blank L.M., Schmid A. Quantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production. Biotechnol. Bioeng. 2010, 107:357-368.
33. Higgins, D.R., Cregg, J.M., 1998. Pichia protocols. Methods in Molecular Biology, Humana Press, Totowa, USA.
34. Hohenblum H., Borth N., Mattanovich D. Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J. Biotechnol. 2003, 102:281-290.
35. Hohenblum H., Gasser B., Maurer M., Borth N., Mattanovich D. Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol. Bioeng. 2004, 85:367-375.
36. Hyka P., Paulová L., Egger M., Melzoch K. A study of the physiological response of Pichia pastoris growing in a continuous culture to an induced stress situation. Chimia 2005, 59:745-748.
37. Hyka P., Zullig T., Ruth C., Looser V., Meier C., Klein J., Melzoch K., Meyer H.P., Glieder A., Kovar K. Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high cell density fed-batch cultures. Appl. Environ. Microbiol. 2010, 76:4486-4496.
38. Inan M., Meagher M.M. Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris. J. Biosci. Bioeng. 2001, 92:585-589.
39. Invitrogen, 2002. Pichia fermentation process guidelines. Catalog No. K1719-91, pp. 1-11.
40. Issaly N., Solsona O., Joudrier P., Gautier M.F., Moulin G., Boze H. Optimization of the wheat puroindoline - a production in Pichia pastoris. J. Appl. Microbiol. 2001, 90:397-406.
41. Jahic M., Gustavsson M., Jansen A.K., Martinelle M., Enfors S.O. Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J. Biotechnol. 2003, 102:45-53.
42. Jahic M., Wallberg F., Bollok M., Garcia P., Enfors S.O. Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microb. Cell Fact. 2003, 2:6-17.
43. + strain. Enzyme Microb. Technol. 2006, 39:936-944.
44. Jungo C., Marison I., von Stockar U. Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: a quantitative study based on concentration gradients in transient continuous cultures. J. Biotechnol. 2007, 128:824-837.
45. Jungo C., Marison I., von Stockar U. Regulation of alcohol oxidase of a recombinant Pichia pastoris Mut+ strain in transient continuous cultures. J. Biotechnol. 2007, 130:236-246.
46. Katakura Y., Zhang W.H., Zhuang G.Q., Omasa T., Kishimoto M., Goto W., Suga K.I. Effect of methanol concentration on the production of human beta(2)-glycoprotein I domain V by a recombinant Pichia pastoris: a simple system for the control of methanol concentration using a semiconductor gas sensor. J. Ferment. Bioeng. 1998, 86:482-487.
47. Keil B., Sorm F., Holeysovsky V., Kostka V., Meloun B., Mikes O., Tomasek V., Vanecek J. On proteins 41. On the partial structures of bovine chymotrypsinogen and trypsinogen. Collect. Czech. Chem. Commun. 1959, 24:3491-3493.
48. Khatri N.K., Hoffmann F. Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris. Biotechnol. Bioeng. 2006, 93:871-879.
49. Kovar K., Looser V., Hyka P., Merseburger T., Meier C. Recombinant yeast technology at the cutting edge: robust tools for both designed catalysts and new biologicals. Chimia 2010, 64:813-818.
50. Lu Chau T., Guillan A., Roca E., Nunez M.J., Lema J.M. Population dynamics of a continuous fermentation of recombinant Saccharomyces cerevisiae using flow cytometry. Biotechnol. Progr. 2001, 17:951-957.
51. Mattanovich D., Callewaert N., Rouze P., Lin Y.C., Graf A., Redl A., Tiels P., Gasser B., De Schutter K. Open access to sequence: browsing the Pichia pastoris genome. Microb. Cell. Fact. 2009, 8.
52. Mattanovich D., Gasser B., Hohenblum H., Sauer M. Stress in recombinant protein producing yeasts. J. Biotechnol. 2004, 113:121-135.
53. Mattanovich D., Graf A., Stadlmann J., Dragosits M., Redl A., Maurer M., Kleinheinz M., Sauer M., Altmann F., Gasser B. Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris. Microb. Cell. Fact. 2009, 8:29.
54. Maurer M., Kuehleitner M., Gasser B., Mattanovich D. Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris. Microb. Cell. Fact. 2006, 5:37.
55. Nakano A., Lee C.Y., Yoshida A., Matsumoto T., Shiomi N., Katoh S. Effects of methanol feeding methods on chimeric alpha-amylase expression in continuous culture of Pichia pastoris. J. Biosci. Bioeng. 2006, 101:227-231.
56. Okabayashi, K., Ohmura, T., Yokoyama, K., Kawabe, H., 1997. Mutant strain of Pichia pastoris which utilizes methanol in the presence of glucose. US Patent 5,643,792.
57. Ruth C., Glieder A. Perspectives on synthetic promoters for biocatalysis and biotransformation. Chembiochem 2010, 11:761-765.
58. Schenk J., Balazs K., Jungo C., Urfer J., Wegmann C., Zocchi A., Marison I.W., von Stockar U. Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut(+) strain. Biotechnol. Bioeng. 2008, 99:368-377.
59. Sola A., Jouhten P., Maaheimo H., Sanchez-Ferrand F., Szyperski T., Ferrer P. Metabolic flux profiling of Pichia pastoris grown on glycerol/methanol mixtures in chemostat cultures at low and high dilution rates. Microbiol. SGM 2007, 153:281-290.
60. Stadlmayr G., Mecklenbrauker A., Rothmuller M., Maurer M., Sauer M., Mattanovich D., Gasser B. Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production. J. Biotechnol. 2010, 150:519-529.
61. Stratton J., Chiruvolu V., Meagher M. High cell-density fermentation. Method Mol. Biol. 1998, 103:107-120.
62. Tolner B., Smith L., Begent R.H., Chester K.A. Production of recombinant protein in Pichia pastoris by fermentation. Nat. Protoc. 2006, 1:1006-1021.
63. Tschopp J.F., Brust P.F., Cregg J.M., Stillman C.A., Gingeras T.R. Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res. 1987, 15:3859-3876.
64. Waterham H.R., Digan M.E., Koutz P.J., Lair S.V., Cregg J.M. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene 1997, 186:37-44.
65. Wei C., Zhou X., Zhang Y. Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme. Appl. Microbiol. Biotechnol. 2008, 78:257-264.
66. Werten M.W., de Wolf F.A. Reduced proteolysis of secreted gelatin and Yps1-mediated alpha-factor leader processing in a Pichia pastoris kex2 disruptant. Appl. Environ. Microbiol. 2005, 71:2310-2317.
67. Xiao A., Zhou X., Zhou L., Zhang Y. Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation. Appl. Microbiol. Biotechnol. 2006, 72:837-844.
68. Xie J.L., Zhou Q.W., Peng D., Gan R.B., Qin Y. Use of different carbon sources in cultivation of recombinant Pichia pastoris for angiostatin production. Enzyme Microb. Technol. 2005, 36:210-216.
69. Yamawaki S., Matsumoto T., Ohnishi Y., Kumada Y., Shiomi N., Katsuda T., Lee E.K., Katoh S. Production of single-chain variable fragment antibody (scFv) in fed-batch and continuous culture of Pichia pastoris by two different methanol feeding methods. J. Biosci. Bioeng. 2007, 104:403-407.
70. Yano T., Yurimoto H., Sakai Y. Activation of the oxidative stress regulator PpYap1 through conserved cysteine residues during methanol metabolism in the yeast Pichia pastoris. Biosci. Biotechnol. Biochem. 2009, 73:1404-1411.
71. Zhang W., Bevins M.A., Plantz B.A., Smith L.A., Meagher M.M. Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol. Bioeng. 2000, 70:1-8.
72. Zhang W.H., Liu C.P., Inan M., Meagher M.M. Optimization of cell density and dilution rate in Pichia pastoris continuous fermentations for production of recombinant proteins. J. Ind. Microbiol. Biotechnol. 2004, 31:330-334.
73. Zhang W.H., Potter K.J.H., Plantz B.A., Schlegel V.L., Smith L.A., Meagher M.M. Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement. J. Ind. Microbiol. Biotechnol. 2003, 30:210-215.