Recombinant protein expression in Pichia pastoris

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 16, Issue 1, 2000, Pages 23-52

  Cregg, James M ^a   Cereghino, Joan Lin ^b   Shi, Jianying ^b   Higgins, David R ^c  

^a KECK GRADUATE INSTITUTE   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^c PFIZER INC   (United States)

Author keywords

Fermentation; Foreign gene expression; Heterologous protein production; Methylotrophic yeast; Pichia methanolica; Pichia pastoris; Yeast protein expression

Indexed keywords

METHANOL; RECOMBINANT PROTEIN;

ANIMAL FOOD; CYTOLOGY; FERMENTATION; GENE EXPRESSION; MEDICAL RESEARCH; MOLECULAR BIOLOGY; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PROCESSING; REVIEW; YEAST;

ALCOHOL OXIDOREDUCTASES; ANIMALS; CLONING, MOLECULAR; FERMENTATION; GENETIC VECTORS; GENOTYPE; HUMANS; MOLECULAR BIOLOGY; PHENOTYPE; PICHIA; RECOMBINANT PROTEINS;

EID: 0033753671     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:16:1:23     Document Type: Review

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16. Development of yeast transformation systems and construction of methanol-utilization-defective mutants of Pichia pastoris gene disruption
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20. The significance of peroxisomes in the metabolism of one-carbon compounds in yeasts
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23. Protocol for efficient secretion of HSA developed from Pichia pastoris
24. High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast, Pichia pastoris
25. Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fed-batch fermentations
26. High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast Pichia pastoris
27. Production of mouse epidermal growth factor in yeast: High-level secretion using Pichia pastoris strains containing multiple gene copies
28. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter
29. A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris
30. High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris
31. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression
32. High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene
33. Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: High-level production and immunological properties
34. Antibody screening for secreted proteins expressed in Pichia pastoris
35. Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris
36. The solution structure of type II antifreeze protein reveals a new member of the lectin family
37. Independently melting modules and highly structured intermodular junctions within complement receptor type 1
38. Structural analysis of the CD5 antigen - Expression, disulphide bond analysis and physical characterization of the CD5 scavenger receptor superfamily domain 1
39. Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1
40. NMR studies of a viral protein that mimics the regulators of complement activation
41. Isotopically labeled bovine beta-lactoglobulin for NMR studies expressed in Pichia pastoris
42. Complete assignment of 1H, 13C and 15N chemical shifts for bovine beta-lactoglobulin: Secondary structure and topology of the native state is retained in a partially unfolded form
43. High-level expression of bovine beta-lactoglobulin in Pichia pastoris and characterization of its physical properties
44. High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements
45. Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation
46. NMR analysis of the N-terminal SRCR domain of human CD5: Engineering of a glycoprotein for superior characteristics in NMR experiments
47. High-level secretion in Pichia pastoris and biochemical characterization of the recombinant kringle 2 domain of tissue-type plasminogen activator
48. Characterization of the extracellular ligand-binding domain of the type II activin receptor
49. Three-finger toxin fold for the extracellular ligand-binding domain of the type II activin receptor serine kinase
50. Development of the methylotrophic yeast, Pichia methanolica, for the expression of the 65-kilodalton isoform of human glutamate decarboxylase
51. Recombinant protein expression in Pichia methanolica
52. Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris
53. Efficient expression and secretion of recombinant alpha amylase in Pichia pastoris using two different signal sequences
54. The dacA gene of Bacillus stearothemophilus coding for D-alanine carboxypeptidase: Cloning, structure and expression in Escherichia coli and Pichia pastoris
55. Development of recombinant vaccines for botulinum neurotoxin
56. Production and purification of the heavy-chain fragment C of botulinum neurotoxin, serotype B, expressed in the methylotrophic yeast Pichia pastoris
57. Purification, potency, and efficacy of the botulinum neurotoxin type A binding domain from Pichia pastoris as a recombinant vaccine candidate
58. Cloning, sequencing, and expression of an Escherichia coli acid phosphatase/phytase gene (appA2) isolated from pig colon
59. Expression and alteration of the S2 subsite of the Leishmania major cathepsin B-like cysteine protease
60. Glycosylation of asparagine-28 of recombinant staphylokinase with high-mannose-type oligosaccharides results in a protein with highly attenuated plasminogen activator activity
61. Expression of streptokinase in Pichia pastoris yeast
62. Inhibition of Aspergillus serine proteinase by Streptomyces subtilisin inhibitor and high-level expression of this inhibitor in Pichia pastoris
63. Cloning of clustered Streptomyces viridosporus T7A lignocellulose catabolism genes encoding peroxidase and endoglucanase and their extracellular expression in Pichia pastoris
64. The conformation of purified Toxoplasma gondii SAG1 antigen, secreted from engineered Pichia pastoris, is adequate for serorecognition and cell proliferation
65. Production of Vibrio cholerae accessory cholera enterotoxin (Ace) in the yeast Pichia pastoris
66. Isolation and expression of a cDNA clone encoding an Alternaria alternata Alt a 1 subunit
67. Overexpression and characterization of Aspergillus awamori wild-type and mutant glucoamylase secreted by the methylotrophic yeast Pichia pastoris: Comparison with wild-type recombinant glucoamylase produced using Saccharomyces cerevisiae and Aspergillus niger as hosts
68. Expression in Pichia pastoris and purification of Aspergillus awamori glucoamylase catalytic domain
69. Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus
70. Dipeptidyl-peptidase IV secreted by Aspergillus fumigatus, a fungus pathogenic to humans
71. Biochemical and antigenic characterization of a new dipeptidyl-peptidase isolated from Aspergillus fumigatus
72. Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: The Lys-Arg motif is required for maturation
73. Role of glycosylation in the functional expression of an Aspergillus niger phytase (phyA) in Pichia pastoris
74. Cloning and expression of Candida guilliermondii xylose reductase gene (xyl1) in Pichia pastoris
75. Design, total synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase
76. Cloning of a novel constitutively expressed pectate lyase gene pelB from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris
77. Identification of a novel pelD gene expressed uniquely in planta by Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of its protein product as an endopectate lyase
78. High-level production of recombinant Geotrichum candidum lipases in yeast Pichia pastoris
79. Overexpression in Pichia pastoris and crystallization of an elicitor protein secreted by the phytopathogenic fungus, Phytophthora cryptogea
80. Functional expression of Rhizopus oryzae lipase in Pichia pastoris: High-level production and some properties
81. Ktr1p is an alpha-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recombinant Ktr1p and Kre2p/Mnt1p produced in Pichia pastoris
82. Riboflavin 5'-hydroxymethyl oxidation. Molecular cloning, expression, and glycoprotein nature of the 5'-aldehyde-forming enzyme from Schizophyllum commune
83. Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris
84. A new tool for studying the molecular architecture of the fungal cell wall: One-step purification of recombinant trichoderma beta-(1-6)-glucanase expressed in Pichia pastoris
85. Hexose oxidase from the red alga Chondrus crispus. Purification, molecular cloning, and expression in Pichia pastoris
86. A group of alpha-1,4-glucan lyases and their genes from the red alga Gracilariopsis lemaneiformis: Purification, cloning, and heterologous expression
87. High-level expression of Plasmodium vivax apical membrane antigen 1 (AMA-1) in Pichia pastoris: Strong immunogenicity in Macaca mulatta immunized with P. vivax AMA-1 and adjuvant SBAS2
88. Expression of Reticulomyxa filosa tubulins in Pichia pastoris: Regulation of tubulin pools
89. Cloning, expression, purification, and characterization of the acid alpha-mannosidase from Trypanosoma cruzi
90. Recombinant expression of alliin lyase from garlic (Allium sativum) in bacteria and yeasts
91. Analysis of wild-type and mutant plant nitrate reductase expressed in the methylotrophic yeast Pichia pastoris
92. Identification in vitro of a post-translational regulatory site in the hinge 1 region of Arabidopsis nitrate reductase
93. Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris
94. Overexpression, purification, and characterization of recombinant barley alpha-amylases 1 and 2 secreted by the methylotrophic yeast Pichia pastoris
95. Expression of enzymatically active, recombinant barley alpha-glucosidase in yeast and immunological detection of alpha-glucosidase from seed tissue
96. High-level expression and purification of coffee bean alpha-galactosidase produced in the yeast Pichia pastoris
97. Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from cardoon (Cynara cardunculus)
98. Sequence polymorphism of the group 1 allergen of Bermuda grass pollen
99. Cloning and expression in yeast Pichia pastoris of a biologically active form of Cyn d 1, the major allergen of Bermuda grass pollen
100. Functional phytohaemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris: Correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide
101. High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts
102. cDNA cloning of the 43-kDa latex allergen Hev b 7 with sequence similarity to patatins and its expression in the yeast Pichia pastoris
103. Cloning of the patatin-like latex allergen Hev b 7, its expression in the yeast Pichia pastoris and its immunological characterization
104. Isolation of a gene encoding a glycosylated cytokinin oxidase from maize
105. Large-scale generation of affinity-purified recombinant phytochrome chromopeptide
106. Effect of chromophore exchange on the resonance Raman spectra of recombinant phytochromes
107. Chromophore incorporation. Pr to Pfr kinetics, and Pfr thermal reversion of recombinant N-terminal fragments of phytochrome A and B chromoproteins
108. Production and detailed characterization of biologically active olive pollen allergen Ole e 1 secreted by the yeast Pichia pastoris
109. Secretion, purification and activity of two recombinant pepper endo-beta-1,4-glucanases expressed in the yeast Pichia pastoris
110. Identification and cloning of prs a 1, a 32-kDa endochitinase and major allergen of avocado, and its expression in the yeast Pichia pastoris
111. Grass group I allergens (beta-expansins) are novel, papain-related proteinases
112. Recombinant type A and B phytochromes from potato. Transient absorption spectroscopy
113. Cloning and expression of ragweed allergen Amb a 6
114. Purification and characterization of a soybean root nodule phosphatase expressed in Pichia pastoris
115. High-level production of spinach glycolate oxidase in the methylotrophic yeast Pichia pastoris: Engineering a biocatalyst
116. Engineering Pichia pastoris for biocatalysis: Co-production of two active enzymes
117. Efficient expression of the gene for spinach phosphoribulokinase in Pichia pastoris and utilization of the recombinant enzyme to explore the role of regulatory cysteinyl residues by site-directed mutagenesis
118. Processing, disulfide pattern, and biological activity of a sugar beet defensin. AX2, expressed in Pichia pastoris
119. Comparison of natural and recombinant isoforms of grass pollen allergens
120. Characterization of LeMir, a root-knot nematode-induced gene in tomato with an encoded product secreted from the root
121. High-level secretion of a wheat lipid transfer protein in Pichia pastoris
122. Macromolecular antimicrobial glycoprotein, achacin, expressed in a methylotrophic yeast Pichia pastoris
123. High-level expression of recombinant Aplysia ADP-ribosyl cyclase in Pichia pastoris by fermentation
124. Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells
125. Heterologous expression of an engineered truncated form of human Lewis fucosyltransferase (Fuc-TIII) by the methylotrophic yeast Pichia pastoris
126. Adjuvant and immuno-stimulating properties of the recombinant Bm86 protein expressed in Pichia pastoris
127. Effect of particulation on the immunogenic and protective properties of the recombinant Bm86 antigen expressed in Pichia pastoris
128. High level expression of the B. microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle
129. High-level expression of cockroach allergen, Bla g 4, in Pichia pastoris
130. Drosophila melanogaster angiotensin I-converting enzyme expressed in Pichia pastoris resembles the C domain of the mammalian homologue and does not require glycosylation for secretion and enzymic activity
131. Sequencing and functional expression of the malonyl-CoA-sensitive carnitine palmitoyltransferase from Drosophila melanogaster
132. The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome - The PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family
133. Large-scale production in Pichia pastoris of the recombinant vaccine Gavac against cattle tick
134. Expression of a synthetic gene encoding the anticoagulant-antimetastatic protein ghilanten by the methylotropic yeast Pichia pastoris
135. Production and purification of recombinant hirudin expressed in the methylotrophic yeast Pichia pastoris
136. Optimization of the production of a honeybee odorant-binding protein by Pichia pastoris
137. Cloning and expression of a queen pheromone-binding protein in the honeybee: An olfactory-specific, developmentally regulated protein
138. Cloning, expression, and properties of a nonneuronal secreted acetylcholinesterase from the parasitic nematode Nippostrongylus brasiliensis
139. Production of synthetic spider dragline silk protein in Pichia pastoris
140. Expressions of recombinant venom allergen, antigen 5 of yellowjacket (Vespula vulgaris) and paper wasp (Polistes annularis), in bacteria or yeast
141. Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris
142. Expression of biologically active beta subunit of bovine follicle-stimulating hormone in the methylotrophic yeast Pichia pastoris
143. The bovine IFN-omega 1 is biologically active and secreted at high levels in the yeast Pichia pastoris
144. Continuous production of a novel lysozyme via secretion from the yeastm, Pichia pastoris
145. Functional expression of bovine opsin in the methylotrophic yeast Pichia pastoris
146. High-level secretion of biologically active aprotinin from the yeast Pichia pastoris
147. Study of putative glycosylation site in bovine beta-casein introduced by PCR-based site-directed mutagenesis
148. A refined kinetic analysis of plasminogen activation by recombinant bovine tissue-type plasminogen activator indicates two interconvertible activator forms
149. Sequence, S-S bridges, and spectra of bovine transcobalamin expressed in Pichia pastoris
150. Physiological effects and adjuvanticity of recombinant brushtail possum TNF-alpha
151. Expression and processing of vertebrate acetylcholinesterase in the yeast Pichia pastoris
152. Expression, purification, and characterization of recombinant alpha-N-acetylgalactosaminidase produced in the yeast Pichia pastoris
153. Cloning and expression of acetylcholinesterase from Electrophorus. Splicing pattern of the 3' exons in vivo and in transfected mammalian cells
154. Molecular and crystal properties of Bos d 2, an allergenic protein of the lipocalin family
155. Comparative biochemical and pharmacological characterization of the mouse 5HT5A 5-hydroxytryptamine receptor and the human beta 2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris
156. High-yield secretion of recombinant gelatins by Pichia pastoris
157. Production and characterization of recombinant active mouse gelatinase B from eukaryotic cells and in vivo effects after intravenous administration
158. Cloning, expression, purification, and characterization of the murine lysosomal acid alpha-mannosidase
159. Expression of a lipocalin in Pichia pastoris: Secretion, purification and binding activity of a recombinant mouse major urinary protein
160. Mutations in either nucleotide-binding site of P-glycoprotein (Mdr3) prevent vanadate trapping of nucleotide at both sites
161. High-level expression of mouse Mdr3 P-glycoprotein in yeast Pichia pastoris and characterization of ATPase activity
162. Mutations in the nucleotide-binding sites of P-glycoprotein that affect substrate specificity modulate substrate-induced adenosine triphosphatase activity
163. High-level secretion of two antibody single chain Fv fragments by Pichia pastoris
164. Disruption of the KEXI gene in Pichia pastoris allows expression of full-length murine and human endostatin
165. Purification, crystallization and preliminary X-ray crystallographic analysis of recombinant murine Golgi mannosidase IA, a class I alphamannosidase involved in Asn-linked oligosaccharide maturation
166. Functional and receptor binding characterization of recombinant murine macrophage inflammatory protein 2: Sequence analysis and mutagenesis identify receptor binding epitopes
167. Expression and secretion of a biologically active glycoprotein hormone, ovine follicle-stimulating hormone, by Pichia pastoris
168. Mapping the pro-region of carboxypeptidase B by protein engineering. Cloning, overexpression, and mutagenesis of the porcine proenzyme
169. High-level secretion of biologically active recombinant porcine follicle-stimulating hormone by the methylotrophic yeast Pichia pastoris
170. Cloning, sequencing, and recombinant expression of the porcine inhibitor of carbonic anhydrase: A novel member of the transferrin family
171. Expression, purification, and characterization of porcine leukocyte 12-lipoxygenase produced in the methylotrophic yeast, Pichia pastoris
172. Expression and functional characterization of the mammalian intestinal peptide transporter PEPT1 in the methylotrophic yeast Pichia pastoris
173. Expression of the mammalian renal peptide transporter PEPT2 in the yeast Pichia pastoris and applications of the yeast system for functional analysis
174. Generation of rabbit monoclonal antibody fragments from a combinatorial phage display library and their production in the yeast Pichia pastoris
175. Expression and secretion of rabbit plasma cholesteryl ester transfer protein by Pichia pastoris
176. Synthesis and cleavage - Secretion of enzymatically active rabbit angiotensin-converting enzyme in Pichia pastoris
177. Functional expression of a mammalian acetylcholinesterase in Pichia pastoris: Comparison to acetylcholinesterase, expressed and reconstituted from Escherichia coli
178. Production of the rat complement regulator, Crry, as an active soluble protein in Pichia pastoris
179. The Golgi sialoglycoprotein MG160, expressed in Pichia pastoris, does not require complex carbohydrates and sialic acid for secretion and basic fibroblast growth factor binding
180. Recombinant HMG1 protein produced in Pichia pastoris: A nonviral gene delivery agent
181. Functional studies of yeast-expressed human heart muscle carnitine palmitoyltransferase I
182. Functional characterization of mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris
183. Expression, crystallization and preliminary X-ray analysis of the two amino-terminal Ig domains of the neural cell adhesion molecule (NCAM)
184. Characterization of the reductase domain of rat neuronal nitric oxide synthase generated in the methylotrophic yeast Pichia pastoris. Calmodulin response is complete within the reductase domain itself
185. Peroxisomal multifunctional enzyme of beta-oxidation metabolizing D-3-hydroxyacyl-CoA esters in rat liver: Molecular cloning, expression and characterization
186. An NMR-based identification of peptide fragments mimicking the interactions of the cathepsin B propeptide
187. Crystallization of rat procathepsin B
188. Biosynthetic production of type II fish antifreeze protein: Fermentation by Pichia pastoris
189. Functional expression of recombinant spiny dogfish shark (Squalus acanthias) cytochrome P450c17 (17 alpha-hydroxylase/C17,20-lyase) in yeast (Pichia pastoris)
190. Overexpression of active Syrian golden hamster prion protein PrP(c) as a glutathione S-transferase fusion in heterologous systems
191. Molecular cloning, chromosomal mapping and tissue-specific expression of a novel human alphal,2-mannosidase gene involved in N-glycan maturation
192. Structural studies of alpha-N-acetylgalactosaminidase: Effect of glycosylation on the level of expression, secretion efficiency, and enzyme activity
193. Glycosylation of human alpha 1-antitrypsin in Saccharomyces cerevisiae and methylotrophic yeasts
194. Characterization of wild-type and mutant alpha 2-antiplasmins: Fibrinolysis enhancement by reactive site mutant
195. Expression and pharmacological characterization of the human square-opioid receptor in the methylotrophic yeast Pichia pastoris
196. Purification of native and recombinant double-stranded RNA-specific adenosine deaminases
197. Processing of the Alzheimer's disease amyloid precursor protein in Pichia pastoris: Immunodetection of alpha-, beta-, and gamma-secretase products
198. Expression and analysis of heparin-binding regions of the amyloid precursor protein of Alzheimer's disease
199. Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth
200. Expression of human amyloid precursor protein ectodomains in Pichia pastoris: Analysis of culture conditions, purification, and characterization
201. Subcellular localization of the Alzheimer's disease amyloid precursor protein and derived polypeptides expressed in a recombinant yeast system
202. Atypical processing of amyloid precursor fusion protein by proteolytic activity in Pichia pastoris
203. Expression, purification, and neurotrophic activity of amyloid precursor protein-secreted forms produced by yeast
204. Production of recombinant human bile salt stimulated lipase and its variant in Pichia pastoris
205. Improved tumour targeting by disulphide stabilized diabodies expressed in Pichia pastoris
206. Intracellular expression and purification of the c-kit receptor kinase domain in Pichia pastoris
207. A transforming mutation enhances the activity of the c-Kit soluble tyrosine kinase domain
208. Expression; purification, and characterization of a two domain carcinoembryonic antigen minigene (N-A3) in Pichia pastoris. The essential role of the N-domain
209. Recombinant caspase-3 expressed in Pichia pastoris is fully activated and kinetically indistinguishable from the native enzyme
210. Expression of human cathepsin K in Pichia pastoris and preliminary crystallographic studies of an inhibitor complex
211. Characterization of novel cathepsin K mutations in the pro and mature polypeptide regions causing pycnodysostosis
212. Autocatalytic processing of recombinant human procathepsin L. Contribution of both intermolecular and unimolecular events in the processing of procathepsin L in vitro
213. Potency and selectivity of the cathepsin L propeptide as an inhibitor of cysteine proteases
214. Human cathepsin V functional expression, tissue distribution, electrostatic surface potential, enzymatic characterization, and chromosomal localization
215. Human cathepsin X: A cysteine protease with unique carboxypeptidase activity
216. Large-scale production of human CD38 in yeast by fermentation
217. Expression of trimeric CD40 ligand in Pichia pastoris: Use of a rapid method to detect high-level expressing transformants
218. Costimulation of T cell proliferation by a chimeric B7-2 antibody fusion protein specifically targeted to cells expressing the erbB2 proto-oncogene
219. Hyperexpression of biologically active human chorionic gonadotropin using the methylotropic yeast Pichia pastoris
220. Hemagglutination inhibition of Cromer blood group antibodies with soluble recombinant decay-accelerating factor
221. Intracellular production of a major cytomegalovirus antigenic protein in the methylotrophic yeast Pichia pastoris
222. Interaction between echovirus 7 and its receptor, decay-accelerating factor (CD55): Evidence for a secondary cellular factor in A-particle formation
223. Two forms of human double-stranded RNA-specific editase 1 (hRED1) generated by the insertion of an Alu cassette
224. Zinc-binding of endostatin is essential for its angiogenic activity
225. Engineering a soluble extracellular erythropoietin receptor (EPObp) in Pichia pastoris to eliminate microheterogeneity, and its complex with erythropoietin
226. Lethal effect of recombinant human Fas ligand in mice pretreated with Propionibacterium acnes
227. The polymerization pocket 'a' within the carboxy-terminal region of the gamma chain of human fibrinogen is adjacent to but independent from the calcium-binding site
228. Production and purification of human fibroblast collagenase (MMP-1) expressed in the methylotrophic yeast Pichia pastoris
229. Crystal structure of a recombinant alpha EC domain from human fibrinogen-420
230. Secretion of human intracellular aspartic proteinase cathepsin E expressed in the methylotrophic yeast, Pichia pastoris and characterization of produced recombinant cathepsin E
231. Expression of human gelatinase B in Pichia pastoris
232. Expression and purification of recombinant human granzyme B from Pichia pastoris
233. Reconstitution of highly expressed human heart muscle carnitine palmitoyltransferase I
234. Secretory expression and characterization of insulin in Pichia pastoris
235. Secretion of recombinant human insulin-like growth factor I (IGF-1)
236. Human IFN gamma receptor cytoplasmic domain: Expression and interaction with HuIFN gamma
237. Expression, purification, and characterization of interferon-tau produced in Pichia pastoris grown in a minimal medium
238. Expression of human interleukin-17 in Pichia pastoris: Purification and characterization
239. Production and characterization of recombinant human proteinase inhibitor 6 expressed in Pichia pastoris
240. High level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid beta-protein precursor
241. Evidence for the formation of a heterotrimeric complex of leukaemia inhibitory factor with its receptor subunits in solution
242. Functional expression of soluble forms of human CD38 in Escherichia coli and Pichia pastoris
243. Cloning, expression, purification, and characterization of the human broad specificity lysosomal acid alpha-mannosidase
244. Expression and characterization of recombinant mast cell tryptase
245. Recombinant human mast cell tryptase beta: Stable expression in Pichia pastoris and purification of fully active enzyme
246. Expression of recombinant HLA-DR2 molecules. Replacement of the hydrophobic transmembrane region by a leucine zipper dimerization motif allows the assembly and secretion of soluble DR alpha-beta heterodimers
247. Vl-linker-Vh orientation-dependent expression of single chain Fv-containing an engineered disulfide-stabilized bond in the framework regions
248. Expression of human monocyte chemoattractant protein-1 in the yeast Pichia pastoris
249. Expression of a human mutant monocyte chemotactic protein 3 in Pichia pastoris and characterization as an MCP-3 receptor antagonist
250. Heterologous overexpression of human NEFA and studies on the two EF-hand calcium-binding sites
251. The first immunoglobulin-like neural cell adhesion molecule (NCAM) domain is involved in double-reciprocal interaction with the second immunoglobulin-like NCAM domain and in heparin binding
252. NonO enhances the association of many DNA-binding proteins to their targets
253. The N-terminus of gp130 is critical for the formation of the high-affinity interleukin-6 receptor complex
254. Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris
255. Human pancreatic triglyceride lipase expressed in yeast cells: Purification and characterization
256. Synthesis of amidrazones using an engineered papain nitrile hydratase
257. Human placental alkaline phosphatase: Expression in Pichia pastoris, purification and characterization of the enzyme
258. Cloning, expression, purification, and immunocharacterization of placental protein-14
259. O-Mannosylation of Pichia pastoris cellular and recombinant proteins
260. A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer
261. Enhancement through mutagenesis of the binding of the isolated kringle 2 domain of human plasminogen to omega-amino acid ligands and to an internal sequence of a Streptococcal surface protein
262. Overexpression of human procarboxypeptidase A2 in Pichia pastoris and detailed characterization of its activation pathway
263. Role of occluding loop in cathepsin B activity
264. Purification and characterization of human procolipase expressed in yeast cells
265. Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins
266. Recombinant proteinase 3 (Wegener's antigen) expressed in Pichia pastoris is functionally active and is recognized by patient sera
267. Expression, purification, and inhibitory properties of human proteinase inhibitor
268. Expression of a fusion protein of scFv-biotin mimetic peptide for immunoassay
269. In vitro production of recombinant antibody fragments in Pichia pastoris
270. Physicochemical and immunochemical properties of recombinant human serum albumin from Pichia pastoris
271. Complete determination of disulfide forms of purified recombinant human serum albumin, secreted by the yeast Pichia pastoris
272. Analysis of Pichia pastoris components in recombinant human serum albumin by immunological assays and by HPLC with pulsed amperometric detection
273. Structural investigations of a new familial dysalbuminemic hyperthyroxinemia genotype
274. Production and purification of N-terminal half-transfertin in Pichia pastoris
275. X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32
276. Production and isolation of the recombinant N-lobe of human serum transferrin from the methylotrophic yeast Pichia pastoris
277. Heterologous expression of wild type and deglycosylated human sex steroid-binding protein (SBP or SHBG) in the yeast. Pichia pastoris. Characterization of the recombinant proteins
278. Secretion of a variant of human single-chain urokinase-type plasminogen activator without an N-glycosylation site in the methylotrophic yeast. Pichia pastoris and characterization of the secreted product
279. Large-scale expression, purification and characterization of small fragments of thrombomodulin: The roles of the sixth domain and of methionine 388
280. Production of human tissue factor using the Pichia pastoris expression system
281. Expression and characterization of human tissue kallikrein variants
282. Crystal structure of recombinant human tissue kallikrein at 2.0 Å resolution
283. Use of site-specific recombination to regenerate selectable markers
284. Characterization of the acidic oligosaccharides assembled on the Pichia pastoris-expressed recombinant kringle 2 domain of human tissue-type plasminogen activator
285. Glycosylation properties of the Pichia pastoris-expressed recombinant kringle 2 domain of tissue-type plasminogen activator
286. Role of tryptophan-63 of the kringle 2 domain of tissue-type plasminogen activator in its thermal stability, folding, and ligand binding properties
287. Expression of recombinant human soluble type II transforming growth factor-beta receptor in Pichia pastoris and Escherichia coli: Two powerful systems to express a potent inhibitor of transforming growth factor-beta
288. High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris
289. Binding of urokinase-type plasminogen activator-plasminogen activator inhibitor-1 complex to the endocytosis receptors alpha 2-macroglobulin receptor/low-density lipoprotein receptor-related protein and very-low-density lipoprotein receptor involves basic residues in the inhibitor
290. Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast Pichia pastoris: Formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase
291. Secretory production of recombinant urokinase-type plasminogen activator-annexin V chimeras in Pichia pastoris
292. Expression of biologically active human vascular endothelial growth factor in yeast
293. Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin
294. Protection of mice against a lethal influenza challenge by immunization with yeast-derived recombinant influenza neuraminidase
295. Yeast-secreted bovine herpesvirus type 1 glycoprotein D has authentic conformational structure and immunogenecity
296. A chimeric protein comprised of bovine herpesvirus type 1 glycoprotein D and bovine interleukin-6 is secreted by yeast and possesses biological activities of both molecules
297. Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles
298. Altered antigenicity of 'a' determinant variants of hepatitis B virus
299. Quality and authenticity of heterologous proteins synthesized in yeast
300. Expression and characterization of the hepatitis E virus ORF3 protein in the methylotrophic yeast, Pichia pastoris
301. The intracellular production and secretion 52 of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris
302. Production of active polyomavirus large T antigen in yeast Pichia pastoris
303. Characterization of the nucleoside triphosphate phosphohydrolase and helicase activities of the reovirus lambda 1 protein
304. A glycosyl hydrolase activity of mammalian reovirus sigma 1 protein can contribute to viral infection through a mucus layer