Quantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production

Biotechnology and Bioengineering

Volumn 107, Issue 2, 2010, Pages 357-368

  Heyland, Jan ^a   Fu, Jianan ^a   Blank, Lars M ^a   Schmid, Andreas ^a  

^a TU DORTMUND UNIVERSITY   (Germany)

Author keywords

13C flux analysis; Metabolic burden; MFA; Pichia pastoris; Recombinant protein production; TCA cycle

Indexed keywords

FLUX ANALYSIS; METABOLIC BURDEN; MFA; PICHIA PASTORIS; RECOMBINANT PROTEIN PRODUCTIONS; TCA CYCLE; 13C-FLUX ANALYSIS;

ECOLOGY; ENERGY RESOURCES; FERMENTATION; GLUCOSE; METABOLISM; METHANOL; CARBON; CYTOLOGY; EXPERIMENTS; PHYSIOLOGY; RECOMBINANT PROTEINS; YEAST;

PROTEINS; CELLS;

CARBON 13; GLUCOSE; METHANOL; RECOMBINANT PROTEIN; CARBON; FUNGAL PROTEIN;

ARTICLE; BACTERIUM CULTURE; CARBON SOURCE; CELL DENSITY; CELL GROWTH; CITRIC ACID CYCLE; CONTROLLED STUDY; ENERGY RESOURCE; FED BATCH CULTURE; FED BATCH FERMENTATION; FUNGAL BIOMASS; FUNGAL CELL; FUNGAL STRAIN; GROWTH RATE; NONHUMAN; PHYSIOLOGY; PICHIA PASTORIS; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; BIOMASS; FERMENTATION; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; PICHIA; STAINING; CONCENTRATION (PARAMETERS); FED BATCH REACTOR; FUNGAL METABOLISM; FUNGUS GROWTH;

PICHIA PASTORIS;

BIOMASS; CARBON ISOTOPES; CITRIC ACID CYCLE; FERMENTATION; FUNGAL PROTEINS; GLUCOSE; PICHIA; RECOMBINANT PROTEINS; STAINING AND LABELING;

EID: 78149252300     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22836     Document Type: Article

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