Effects of methanol feeding methods on chimeric α-amylase expression in continuous culture of Pichia pastoris

Journal of Bioscience and Bioengineering

Volumn 101, Issue 3, 2006, Pages 227-231

  Nakano, Atsushi ^a   Lee, Chun Yeon ^a   Yoshida, Arei ^a   Matsumoto, Takehiro ^a   Shiomi, Naofumi ^b   Katoh, Shigeo ^a  

^a KOBE UNIVERSITY   (Japan)

^b KOBE COLLEGE   (Japan)

Author keywords

amylase; continuous culture; DO stat; induction with methanol; methylotrophic yeast

Indexed keywords

CONTINUOUS CELL CULTURE; FERMENTATION; GENETIC ENGINEERING; GROWTH KINETICS; METHANOL; PROTEINS; YEAST;

Α-AMYLASE; DO-STAT; INDUCTION WITH METHANOL; METHYLOTROPHIC YEAST;

ENZYME KINETICS;

AMYLASE; HYBRID PROTEIN; METHANOL; RECOMBINANT PROTEIN;

ARTICLE; CELL GROWTH; CHIMERA; CONTROLLED STUDY; DILUTION; FERMENTATION; MOLECULAR MODEL; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; VOLUMETRY; BIOREACTOR; BIOTECHNOLOGY; CELL CULTURE; CHEMISTRY; GENE EXPRESSION; METABOLISM; METHODOLOGY; PICHIA; TIME;

PICHIA; PICHIA PASTORIS;

ALPHA-AMYLASE; BIOREACTORS; BIOTECHNOLOGY; CELLS, CULTURED; FERMENTATION; GENE EXPRESSION; METHANOL; PICHIA; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; TIME FACTORS;

EID: 33745699548     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.101.227     Document Type: Article

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