Production of Single-Chain Variable Fragment Antibody (scFv) in Fed-Batch and Continuous Culture of Pichia pastoris by Two Different Methanol Feeding Methods

Journal of Bioscience and Bioengineering

Volumn 104, Issue 5, 2007, Pages 403-407

  Yamawaki, Shinya ^a   Matsumoto, Takehiro ^a   Ohnishi, Yuka ^a   Kumada, Yoichi ^b   Shiomi, Naofumi ^c   Katsuda, Tomohisa ^a   Lee, Eun Kyu ^d   Katoh, Shigeo ^a  

^a KOBE UNIVERSITY   (Japan)

^b KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^c KOBE COLLEGE   (Japan)

^d Hanyang University   (South Korea)

Author keywords

continuous culture; DO stat; fed batch culture; methanol concentration control; methylotrophic yeast; scFv

Indexed keywords

ANTIBODIES; CELL CULTURE; CELL GROWTH; METHANOL;

FED BATCH CULTURE; METHANOL CONCENTRATION CONTROL; METHYLOTROPHIC YEAST; SINGLE-CHAIN VARIABLE FRAGMENT ANTIBODY;

YEAST;

METHANOL; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY PRODUCTION; ARTICLE; BROTH DILUTION; CONCENTRATION (PARAMETERS); CONTINUOUS CULTURE; CONTROLLED STUDY; FED BATCH CULTURE; FOOD INTAKE; FUNGUS CULTURE; NONHUMAN; PICHIA PASTORIS;

CELL CULTURE TECHNIQUES; FERMENTATION; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; INDUSTRIAL MICROBIOLOGY; METHANOL; PICHIA; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 37049021103     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.104.403     Document Type: Article

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