Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures

Journal of Biotechnology

Volumn 128, Issue 4, 2007, Pages 824-837

  Jungo, Carmen ^a   Marison, Ian ^b   von Stockar, Urs ^a  

^a EPFL   (Switzerland)

^b DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

Avidin; Calorimetry; Mixed substrate; Pichia pastoris; Transient

Indexed keywords

GLYCEROL; GROWTH KINETICS; METHANOL; PROTEINS;

AVIDIN; MIXED SUBSTRATES; PICHIA PASTORIS;

CELL CULTURE;

ALCOHOL OXIDASE; AVIDIN; GLYCEROL; METHANOL;

ANIMAL CELL; ARTICLE; BIOMASS; CELL DENSITY; CONTINUOUS CULTURE; FED BATCH CULTURE; FUNGAL STRAIN; FUNGUS CULTURE; NONHUMAN; OXYGEN CONSUMPTION; PICHIA PASTORIS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; THERMOGENESIS;

ALCOHOL OXIDOREDUCTASES; AVIDIN; BIOREACTORS; GLYCEROL; INDUSTRIAL MICROBIOLOGY; METHANOL; MYCOLOGY; PICHIA; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 33847375437     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.12.024     Document Type: Article

References (43)

1. Babel W., Brinkmann U., and Muller R.H. The auxiliary substrate concept-an approach for overcoming limits of microbial performances. Ada. Biotechnol. 13 (1993) 211-242
2. Birou, B., 1986. PhD Thesis no 612. Etude de la chaleur dégagée par des cultures microbiennes dans un fermenteur de laboratoire. Institute of Chemical and Biological Engineering. Lausanne, Ecole Polytechnique Fédérale de Lausanne, Switzerland.
3. Brinkmann U., Mueller R.H., and Babel W. The growth rate-limiting reaction in methanol-assimilating yeasts. FEMS Microb. Rev. 87 (1990) 261-266
4. Cannizzaro, C., 2002. PhD Thesis no 2620. Spectroscopic monitoring of bioprocesses: a study of carotenoid production by Phaffia Rhodozyma yeast. Institute of Chemical and Biological Engineering. Lausanne, Ecole Polytechnique Fédérale de Lausanne, Switzerland.
5. Cregg J.M., Madden K.R., Barringer K.J., Thill G.P., and Stillman C.A. Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol. cell. Biol. 9 3 (1989) 1316-1323
6. Cregg J.M., Vedvick T.S., and Raschke W.C. Recent advances in the expression of foreign genes in Pichia pastoris. Bio/Technology 11 8 (1993) 905-910
7. Curvers S., Linnemann J., Klauser T., Wandrey C., and Takors R. Recombinant protein production with Pichia pastoris in continuous fermentation-kinetic analysis of growth and product formation. Chem. Ing. Tech. 73 (2001) 1615-1621
8. D'Anjou M.C., and Daugulis A.J. Mixed-feed exponential feeding for fed-batch culture of recombinant methylotrophic yeast. Biotechnol. Lett. 22 (2000) 341-346
9. D'Anjou M.C., and Daugulis A.J. A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol. Bioeng. 72 1 (2001) 1-11
10. Dijken J.P., Otto R., and Harder W. Growth of Hansenula polymorpha in a methanol-limited chemostat. Arch. Microbiol. 111 (1976) 137-144
11. Duboc P., and von Stockar U. Systematic errors in data evaluation due to ethanol stripping and water vaporization. Biotechnol. Bioeng. 58 4 (1998) 428-439
12. Egli T., Bosshard C., and Hamer G. Simultaneous utilization of methanol-glucose mixtures by Hansenula polymorpha in chemostat: influence of dilution rate and mixture composition on utilization pattern. Biotechnol. Bioeng. 28 (1986) 1735-1741
13. Egli T., and Fiechter A. Theoretical analysis of media used in the growth of yeasts on methanol. J. Gen. Microbiol. 123 (1981) 365-369
14. Egli T., Käppeli O., and Fiechter A. Regulatory flexibility of methyltrophic yeasts in chemostat cultures: simultaneous assimilation of glucose and methanol at a fixed dilution rate. Arch. Microbiol. 131 (1982) 1-7
15. Ellis S.B., Brust P.F., Koutz P.J., Waters A.F., Harpold M.M., and Gingeras T.R. Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Mol. cell. Biol. 5 5 (1985) 1111-1121
16. Gurakan T., Marison I.W., von Stockar U., Gustafsson L., and Gnaiger E. Proposals for a standardized sample handling procedure for the determination of elemental composition and enthalpy of combustion of biological material. Thermochim. Acta 172 (1990) 251-266
17. Hellwig S., Emde F., Raven N.P.G., Henke M., van der Logt P., and Fischer R. Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol. Bioeng. 74 4 (2001) 344-352
18. Inan M., and Meagher M.M. The effect of ethanol and acetate on protein expression in Pichia pastoris. J. Biosci. Bioeng. 92 4 (2001) 337-341
19. Jenzsch M., Lange M., Bär J., Rahfeld J.-U., and Lübbert A. Bioreactor retrofitting to avoid aeration with oxygen in Pichia pastoris cultivation processes for re-combinant protein production. Chem. Eng. Res. Des. 82 A9 (2004) 1144-1152
20. + strain. Enzyme Microb. Technol. 39 4 (2006) 936-944
21. Jungo C., Urfer J., Zocchi A., Marison I.W., and von Stockar U. Optimisation of culture conditions with respect to biotin requirement for the production of recombinant avidin in Pichia pastoris. J. Biotechnol. 127 4 (2007) 703-715
22. Kada G., Falk H., and Gruber H.J. Accurate measurement of avidin and streptavidin in crude biofluids with a new, optimized biotin-fluorescein conjugate. Biochim. Biophys. Acta 1427 (1999) 33-43
23. 2-glycoproteinn I Domain V by a recombinant Pichia pastoris: a simple system for the control of methanol concentration using a semiconductor gas sensor. J. Ferment. Bioeng. 86 5 (1998) 482-487
24. Kovarova-Kovar K., and Egli T. Growth kinetics of suspended microbial cells: from single-substrate-controlled growth to mixed-substrate kinetics. Microbiol. Mol. Biol. Rev. 62 3 (1998) 646-666
25. Lendenmann U., and Egli T. Kinetic models for the growth of Escherichia coli with mixtures of sugars under carbon-limited conditions. Biotechnol. Bioeng. 59 1 (1998) 99-107
26. Loewen M.C., Liu X., Davies P.L., and Daugulis A.J. Biosynthetic production of type II fish antifreeze protein: fermentation by Pichia pastoris. Appl. Microbiol. Biotechnol. 48 4 (1997) 480-486
27. Mardon C.J. Automated protocols for the production of recombinant proteins from Pichia pastoris. Aust. Biotechnol. 5 2 (1995) 95-98
28. Marison I., Liu J.S., Ampuero S., von Stockar U., and Schenker B. Biological reaction calorimetry: development of high sensitivity bio-calorimeters. Thermochim. Acta 309 (1998) 157-173
29. Maskow T., and Babel W. Calorimetrically recognized maximum yield of poly-3-hydroxybutyrate (PHB) continuously synthesized from toxic substrates. J. Biotechnol. 77 (2000) 247-253
30. Maskow T., and Babel W. Calorimetric analysis of microorganisms in transient growth states to quantify changes of metabolic fluxes in response to nutrient deficiencies and osmostress. Thermochim. Acta 382 (2002) 229-237
31. McGrew J.T., Leiske D., Dell B., Klinke R., Krasts D., Wee S., Abbott N., Armitage R., and Harrington K. Expression of trimeric CD41 ligand in Pichia pastoris: use of a rapid method to detect high-level expressing transformants. Gene 187 2 (1997) 193-200
32. Meier-Schneiders M., Schäfer F., Grosshans U., and Busch C. Impact of carbon dioxide evolution on the calorimetric monitoring of fermentations. Thermochim. Acta 251 (1995) 85-97
33. Menoud L., Marison I.W., and von Stockar U. On-line detection of baseline variations through torque measurements in isothermal reaction calorimeters. Thermochim. Acta 251 (1995) 79-84
34. Sreekrishna K., Brankamp R.G., Kropp K.E., Blankenship D.T., Tsay J.T., Smith P.L., Wierschke J.D., Subramaniam A., and Birkenberger L.A. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190 (1997) 55-62
35. Stephanopoulos G.N., Aristidou A.A., and Nielsen J. Material balances and data consistency. Metabolic Engineering. Principles and methodologies (1998), Press, Elsevier Science, A. San Diego (USA) 115-146
36. Stratton J., Chiruvolu V., and Meagher M. High cell-density fermentation. Methods in Molecular Biology (1998), D.R. Higgins, J.M.C. Humana Press, Pichia Protocols 107-120
37. Tschopp J.F., Brust P.F., Cregg J.M., Stillman C.A., and Gingeras T.R. Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res. 15 9 (1987) 3859-3876
38. von Stockar U., Gustafsson L., Larsson C., Marison I., Tissot P., and Gnaiger E. Thermodynamic considerations in constructing energy balances for cellular growth. Biochim. Biophys. Acta 1183 (1993) 221-240
39. Wang N.S., and Stephanopoulos G. Application of macroscopic balances to the identification of gross measurement errors. Biotechnol. Bioeng. 25 (1983) 2177-2208
40. Weast R.C. Handbook of Chemistry and Physics (1980), CRC Press, Inc., Boca Raton (Florida)
41. Zhang L., Hywood Potter K.J., Plantz B.A., Schlegel V.L., Smith L.A., and Meagher M.M. Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement. J. Ind. Microbiol. Biot. 30 (2003) 210-215
42. Zinn, M., 1998. PhD Thesis no 12987. Dual (C,N) nutrient limited growth of Pseudomonas oleovorans. Swiss Federal Institute of Environmental Science and Technology (EAWAG). Zurich, Switzerland.
43. Zocchi A., Jobé A.M., Neuhaus J.M., and Ward T.R. Expression and purification of a recombinant avidin with a lowered isoelectric point in Pichia pastoris. Protein Exp. Purif. 32 (2003) 167-174