Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A

Biotechnology and Bioengineering

Volumn 70, Issue 1, 2000, Pages 1-8

  Kim, Namjoon ^a   Choi, Youngjin ^a   Jung, Seunho ^a   Kim, Sunghyun ^a  

^a Konkuk University   (South Korea)

Author keywords

Botulinum neurotoxin; Fed batch; Fermentation; Growth modeling; Optimization; Pichia pastoris

Indexed keywords

COMPOSITION EFFECTS; FERMENTATION; GROWTH (MATERIALS); MATHEMATICAL MODELS; METHANOL; MOLECULAR STRUCTURE;

BOTULINUM NEUROTOXIN; FED-BATCH FERMENTATION;

YEAST;

AMMONIA; BOTULINUM TOXIN A; METHANOL; RECOMBINANT PROTEIN;

ANAEROBIC METABOLISM; ARTICLE; BIOMASS; CELL DENSITY; COMPUTER PROGRAM; CONCENTRATION RESPONSE; CONTROLLED STUDY; FERMENTATION; GROWTH INHIBITION; GROWTH RATE; NONHUMAN; PICHIA PASTORIS; POPULATION MODEL; PROTEIN SYNTHESIS;

BOTULINUM TOXIN TYPE A; CELL DIVISION; DOSE-RESPONSE RELATIONSHIP, DRUG; FERMENTATION; GLYCEROL; KINETICS; METHANOL; MODELS, STATISTICAL; PICHIA; RECOMBINANT PROTEINS; SUPPORT, U.S. GOV'T, NON-P.H.S.; TIME FACTORS;

EID: 0034610097     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20001005)70:1<1::AID-BIT1>3.0.CO;2-Y     Document Type: Article

References (24)

1. Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: Bovine lysozyme
2. Purification, potency, and efficacy of the botulinum neurotoxin type A binding domain from Pichia pastoris as a recombinant vaccine candidate
3. Maximum histidine production by fed-batch culture of Brevibacterium flavum
4. Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli
5. Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase
6. Development of the methylotrophic yeast, Pichia pastoris, as a host system for the production of foreign proteins
7. Recent advances in the expression of foreign genes in Pichia pastoris
8. Production of foreign proteins in the yeast Pichia pastoris
9. Secretion of heterologous proteins from the methylotrophic yeast, Pichia pastoris
10. On-line monitoring and control of methanol concentration in shake-flask culture of Pichia pastoris
11. Characterization of monoclonal antibodies to botulinum type A neurotoxin
12. 2-glycoprotein I domain V by a recombinant Pichia pastoris: A simple system for the control of methanol concentration using a semiconductor gas sensor
13. The most poisonous poison
14. High-level secretion and very efficient isotopic labeling of tick anti-coagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris
15. The enhancement of specific antibody production rate in glucose- and glutamine-controlled fed-batch culture
16. Effects of lactate concentration on hybridoma culture in lactate-controlled fed-batch operation
17. Maximum production strategy for biodegradable copolymer P(HB-co-HV) in fed-batch culture of Alcaligenes eutrophus
18. Optimization and control in fed-batch bioreactors
19. High cell-density fermentation
20. High-level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris
21. Optimization of rice α-amylase production using temperature-sensitive mutants of Saccharomyces cerevisiae for the PHO regulatory system
22. Alcohol oxidase from Hansenula polymorpha CBS 4732
23. Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli