Metabolic flux analysis for recombinant protein production by Pichia pastoris using dual carbon sources: Effects of methanol feeding rate

Biotechnology and Bioengineering

Volumn 105, Issue 2, 2010, Pages 317-329

  Çelik, Eda ^a   Çalik, Pinar ^a   Oliver, Stephen G ^b  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Fed batch; Flux; Metabolism; Methanol; Pichia pastoris; Sorbitol

Indexed keywords

ASSIMILATORY PATHWAYS; BIOPROCESSES; CARBON SOURCE; CELL METABOLISM; DILUTION RATE; FED BATCHES; FED-BATCH; FED-BATCH OPERATION; FEEDING RATE; GLUCONEOGENESIS; HUMAN ERYTHROPOIETIN; METABOLIC EFFECTS; METABOLIC FLUX; METABOLIC FLUX ANALYSIS; METHANOL FEED; P. PASTORIS; PICHIA PASTORIS; RECOMBINANT PROTEIN PRODUCTIONS; SPECIFIC GROWTH RATE; TCA CYCLE;

FEEDING; INTERNET PROTOCOLS; METHANOL; STOICHIOMETRY;

METABOLISM;

FORMALDEHYDE; METHANOL; RECOMBINANT ERYTHROPOIETIN; SORBITOL;

ALCOHOL CONSUMPTION; ARTICLE; CARBON SOURCE; CELL METABOLISM; CITRIC ACID CYCLE; CONTROLLED STUDY; DILUTION; FEEDING; GLUCONEOGENESIS; GLYCOLYSIS; GROWTH RATE; METABOLITE; NONHUMAN; PICHIA PASTORIS; PROTEIN SYNTHESIS; STOICHIOMETRY;

CARBON; ERYTHROPOIETIN, RECOMBINANT; HUMANS; INDUSTRIAL MICROBIOLOGY; METHANOL; MODELS, BIOLOGICAL; PICHIA; SORBITOL;

PICHIA PASTORIS;

EID: 74849113944     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22543     Document Type: Article

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