Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut + strain

Biotechnology and Bioengineering

Volumn 99, Issue 2, 2008, Pages 368-377

  Schenk, Jonas ^a   Balazs, Krisztina ^a   Jungo, Carmen ^a   Urfer, Julien ^b   Wegmann, Carole ^a   Zocchi, Andrea ^c   Marison, Ian W ^d   Von Stockar, Urs ^a  

^a EPFL   (Switzerland)

^b Inotech Labor AG   (Switzerland)

^c Baxter Bioscience   (Switzerland)

^d DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

Avidin; Fed batch; N glycosylation; Pichia pastoris; Specific growth rate

Indexed keywords

CARBOHYDRATES; CELL GROWTH; GLYCOSYLATION; GROWTH RATE;

AVIDIN; FED BATCH CULTIVATIONS; PICHIA PASTORIS; SPECIFIC GROWTH RATE;

PROTEINS;

AVIDIN; CARBOHYDRATE; GLYCAN; GLYCOPROTEIN; MANNOSE; METHANOL; RECOMBINANT PROTEIN;

ARTICLE; FED BATCH CULTURE; FUNGAL STRAIN; FUNGUS GROWTH; GLYCOSYLATION; GROWTH RATE; NONHUMAN; PICHIA PASTORIS; BIOREACTOR; BIOSYNTHESIS; FERMENTATION; GENETICS; METABOLISM; PHYSIOLOGY; PICHIA;

PICHIA PASTORIS;

AVIDIN; BIOREACTORS; FERMENTATION; GLYCOSYLATION; PICHIA; RECOMBINANT PROTEINS;

EID: 38449106761     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21565     Document Type: Article

References (48)

1. Airenne KJ, Oker-Bolm C, Marjomäki VS, Bayer EA, Wilchek M, Kulomaa MS. 1997. Production of biologically active recombinant avidin in baculovirus-infected insect cells. Protein Expr Purif 9(1):100-108.
2. Airenne KJ, Sarkinnen P, Punnonen E-L, Kulomaa MS. 1994. Production of recombinant avidin in Escherichia coli. Gene 144(1):75-80.
3. Andersen DC, Bridges T, Gawlitzek M, Hoy C. 2000. Multiple cell culture factors can affect the glycosylation of Asn-184 in CHO-produced tissue-type plasminogen activator. Biotechnol Bioeng 70(1):25-31.
4. Andrews JF. 1968. A Mathematical model for the continuous culture of microorganisms utilizing inhibitory Substrates. Biotechnol Bioeng X: 707-723.
5. Bruch R, White HB. 1982. Composition and structural heterogeneity of avidin glycopeptides. Biochemistry 21:5334-5341.
6. Cannizzaro C. 2002. Spectroscopic monitoring of bioprocesses: A study of carotenoid production by Phaffia rhodozyma yeast. PhD Thesis No 2620, EPFL (Switzerland).
7. Cereghino JL, Cregg JM. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24(1):45-66.
8. Choi B-K, Bobrowicz P, Davidson RC, Hamilton SR, Kung DH, Li H, Miele RG, Nett JH, Wildt S, Gerngross TU. 2003. Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris. Proc Natl Acad Sci USA 100(9):5022-5027.
9. Cos O, Ramón R, Montesinos JL, Valero F. 2006. Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review. Microb Cell Fact 5:17.
10. Cunha AE, Clemente JJ, Gomes R, Pinto F, Thomaz M, Miranda S, Pinto F, Moosmayer D, Donner P, Carrondo MJT. 2004. Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol Bioeng 86(4):458-467.
11. Daly R, Hearn MTW, 2005. Expression of heterologous proteins in Pichia pastoris: A useful experimental tool in protein engineering and production. J Mol Recognit 18(2):119-138.
12. DeLange RJ. 1970. Egg white avidin. I. Amino acid composition; sequence of the amino- and carboxyl-terminal cyanogen bromide peptides. J Biol Chem 245(5):907-916.
13. Gerngross TU. 2004. Advances in the production of human therapeutic proteins in yeasts and filamentous fungi. Nat Biotechnol 22(11):1409-1414.
14. Goochee CF, Gramer MJ, Andersen DC, Bahr JB, Rasmussen JR. 1991. The oligosaccharides of glycoproteins - Bioprocess factors affecting oligosaccharide structure and their effect on glycoprotein properties. Biotechnology 9(12):1347-1355.
15. Goochee CF, Monica T. 1990. Environmental-effects on protein glycosylation. Biotechnology 8(5):421-427.
16. Grinna LS, Tschopp JF. 1989. Size distribution and general structural features of N-linked oligosaccharides from the methylotrophic yeast, Pichia pastoris. Yeast 5(2):107-115.
17. Gurakan T, Marison IW, von Stockar U, Gustafsson L, Gnaiger E. 1990. Proposals for a standardized sample handling procedure for the determination of elemental composition and enthalpy combustion of biological material. Thermochim Acta 172:251-266.
18. Hellwig S, Emde F, Raven NPG, Henke M, van der Logt P, Fischer R. 2001. Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol Bioeng 74(4):344-352.
19. Hiller Y, Gershoni JM, Bayer EA, Wilchek M. 1987. Biotin binding to avidin. Oligosaccharide side chain not required for ligand association. Biochem J 24(1):167-171.
20. Hong F, Meinander NQ, Jönsson LJ. 2002. Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnol Bioeng 79(4):438-449.
21. Hood EE, Witcher DR, Maddock S, Meyer T, Baszczynski C, Bailey M, Flynn P, Register J, Marshall L, Bond D, et al. 1997. Commercial production of avidin from transgenic maize: Characterization of transformant, production, processing, extraction and purification. Mol Breeding 3:291-306.
22. Invitrogen 2002. Pichia fermentation process guidelines. Carlsbad (CA): Invitrogen.
23. Jenkins N, Parekh RB, James DC. 1996. Getting the glycosylation right: Implications for the biotechnology industry. Nat Biotechnol 14(8):975-981.
24. + strain. Enz Microb Technol 39(4):936-944.
25. Kada G, Falk H, Gruber HJ. 1999. Accurate measurement of avidin and streptavidin in crude biofluids with a new, optimized biotin-fluorescein conjugate. Biochim Biophys Acta 1427:33-43.
26. Kahtri NK, Hoffman F. 2006. Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris. Biotechnol Bioeng 93(5):871-879.
27. Kobayashi K, Kuwae S, Ohya T, Ohda T, Ohyama M, Tomomitsu K. 2000. High level secretion of recombinant human serum albumin by fed-batch fermentation of the methylotrophic yeast, Pichia pastoris, based on optimal methanol feeding strategy. J Biosci Bioeng 90(3):280-288.
28. Li HJ, Sethuraman N, Stadheim TA, Zha DX, Prinz B, Ballew N, Bobrowicz P, Choi BK, Cook WJ, Cukan M, et al. 2006. Optimization of humanized IgGs in glycoengineered Pichia pastoris. Nat Biotechnol 24(2):210-215.
29. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM. 2005. Heterologous protein production using the Pichia pastoris expression system. Yeast 22(4):249-270.
30. Meuwly F, von Stockar U, Kadouri A. 2004. Optimization of the medium perfusion rate in a packed-bed bioreactor charged with CHO cells. Cytotechnology 46(1):37-47.
31. Montesino R, García R, Quintero O, Cremata JA. 1998. Variation in N-Linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris. Protein Expr Purif 14(2):197-207.
32. Nardone E, Rosano C, Santambrogio P, Curnis F, Corti A, Magni F, Siccardi AG, Paganelli G, Losso R, Apreda B, et al. 1998. Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli. Eur J Biochem 256(2):453-460.
33. Ohya T, Ohyama M, Kobayashi K. 2005. Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation. Biotechnol Bioeng 90(7):876-887.
34. Reddy ST, Dahms NM. 2002. High-level expression and characterization of a secreted recombinant cation-dependent mannose 6-phosphate receptor in Pichia pastoris. Protein Expr Purif 26(2):290-300.
35. Rosebrough SF, Hartley DF. 1996. Biochemical modification of streptavidin and avidin: In vitro and in vivo analysis. J Nucl Med 37(8):1380-1384.
36. Schenk J, Marison IW, von Stockar U. 2007. A simple method to monitor and control methanol feeding of Pichia pastoris fermentations using mid-IR spectroscopy. J Biotechnol 128(2):344-353.
37. Sreekrishna K, Brankamp RG, Kropp KE, Blankenship DT, Tsay JT, Smith PL, Wierschke JD, Subramaniam A, Birkenberger LA. 1997. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190(1):55-62.
38. Stephanopoulos GN, Aristidou AA, Nielsden J. 1998. Metabolic engineering: Principles and methodologies. San Diego (CA): Academic Press.
39. Stratton J, Chiruvolu V, Meagher M. 1998. High cell-density fermentation. In: Higgins DR, Cregg JM, editors. Methods in molecular biology. Totowa (NJ): Humana Press p 107-120.
40. Trimble RB, Atkinson PH, Tschopp JF, Townsend RR, Maley F. 1991. Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris. J Biol Chem 266(34):22807-22817.
41. Wilchek M, Bayer EA. 1990. Applications of avidin-biotin technology: Literature survey. In: Wilchek M, Bayer EA, editors. Methods in enzymology. San Diego (CA): Academic Press. Vol. 148: p 14-45.
42. Wildt S, Gerngross TU. 2005. The humanization of N-glycosylation pathways in yeast. Nat Rev Microbiol 3(2):119-128.
43. Woo JH, Liu YY, Neville DM. 2006. Minimization of aggregation of secreted bivalent anti-human T cell immunotoxin in Pichia pastoris bioreactor culture by optimizing culture conditions for protein secretion. J Biotechnol 121:75-85.
44. Yao Z, Zhang M, Sakahara H, Nakamoto Y, Higashi T, Zhao S, Sato N, Arano Y, Konishi J. 1999. The relationship of glycosylation and isoelectric point with tumor accumulation of avidin. J Nucl Med 40(3):479-483.
45. Yuen CT, Storring PL, Tiplady RJ, Izquierdo M, Wait R, Gee CK, Gerson P, Lloyd P, Cremata JA. 2003. Relationships between the N-glycan structures and biological activities of recombinant human erythropoietins produced using different culture conditions and purification procedures. Brit J Haematol 121(3):511-526.
46. Zhang W, Bevins MA, Plantz BA, Smith LA, Meagher MM. 2000. Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol Bioeng 70(1):1-8.
47. Zhang W, Sinha J, Smith LA, Inan M, Meagher MM. 2005. Maximization of production of secreted recombinant proteins in Pichia pastoris fedbatch fermentation. Biotechnol Prog 21(2):386-393.
48. Zocchi A, Jobe A-M, Neuhaus J-M, Ward TR. 2003. Expression and purification of a recombinant avidin with a lowered isoelectric point in Pichia pastoris. Protein Expr Purif 32(2):167-174.