Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry

Journal of Biotechnology

Volumn 102, Issue 3, 2003, Pages 281-290

  Hohenblum, Hubertus ^a   Borth, Nicole ^a   Mattanovich, Diethard ^a  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

Author keywords

Fed batch; Heterologous protein; Immunofluorescence; Methanol induction; Pichia pastoris; Viability

Indexed keywords

CELLS; CYTOLOGY; FERMENTATION; FLUORESCENCE; GENETIC ENGINEERING; IMMUNOLOGY; METHANOL;

CYTOMETRY;

PROTEINS;

METHANOL; PROPIDIUM IODIDE; RECOMBINANT PROTEIN; TRYPSINOGEN;

ARTICLE; BIOTECHNOLOGY; FED BATCH FERMENTATION; FLOW CYTOMETRY; FUNGAL CELL; FUNGAL STRAIN; IMMUNOFLUORESCENCE TEST; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN SECRETION; STRESS;

PICHIA; PICHIA PASTORIS;

EID: 0038364057     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(03)00049-X     Document Type: Article

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