Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations

Biotechnology and Bioengineering

Volumn 74, Issue 4, 2001, Pages 344-352

  Hellwig, Stephan ^a   Emde, Frank ^b   Raven, Nicole P G ^c   Henke, Mario ^a   Van Logt, Paul Der ^b   Fischer, Rainer ^a,d  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b Heinrich Frings GmbH and Co KG   (Germany)

^c UNILEVER RESEARCH   (United Kingdom)

^d IUCT   (Germany)

Author keywords

Methanol sensor; Methylotrophic yeast; Mixed feed fermentation; On line methanol control; Pichia pastoris; Single chain antibody

Indexed keywords

ANTIBODIES; BIOREACTORS; ENZYME KINETICS; FERMENTATION; GLYCEROL; YEAST;

FEED RATES;

GENETIC ENGINEERING;

ANTIBODY; GLYCEROL; METHANOL;

ANTIBODY PRODUCTION; ARTICLE; BIOREACTOR; CELL DENSITY; CONTROLLED STUDY; FERMENTATION; FUNGUS MUTANT; GENE EXPRESSION SYSTEM; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; SURFACE PLASMON RESONANCE;

BIOREACTORS; BIOTECHNOLOGY; CLONING, MOLECULAR; FERMENTATION; GENE EXPRESSION; GLYCEROL; IMMUNOGLOBULIN FRAGMENTS; KINETICS; METHANOL; PICHIA; RECOMBINANT PROTEINS; SURFACE PLASMON RESONANCE; TRANSFORMATION, GENETIC;

PICHIA; PICHIA PASTORIS;

EID: 0035921173     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1125     Document Type: Article

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