Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density Fed-batch cultures

Applied and Environmental Microbiology

Volumn 76, Issue 13, 2010, Pages 4486-4496

  Hyka, Petr ^a,b   Züllig, Thomas ^a   Ruth, Claudia ^c   Looser, Verena ^a   Meier, Christian ^a   Klein, Joachim ^d   Melzoch, Karel ^b   Meyer, Hans Peter ^d   Glieder, Anton ^c   Kovar, Karin ^a  

^a ZURICH UNIVERSITY OF APPLIED SCIENCES   (Switzerland)

^b INSTITUTE OF CHEMICAL TECHNOLOGY   (Czech Republic)

^c GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^d LONZA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BIOPROCESS DEVELOPMENT; BIOPROCESSES; COMBINED EFFECT; CULTURE BROTHS; FED-BATCH CULTURES; FLUORESCENT DYES; HETEROLOGOUS GENES; HETEROLOGOUS PROTEINS; HIGH CELL DENSITY; HORSE-RADISH PEROXIDASE; INDIVIDUAL CELLS; METHANOL UTILIZATION; PH VALUE; PHYSIOLOGICAL STATE; PHYSIOLOGICAL STRESS; PICHIA PASTORIS; PROCESS CONDITION; QUANTITATIVE ASSESSMENTS; RECOMBINANT PROTEIN; RECOMBINANT STRAINS; REPRODUCIBILITIES; STRESS FACTORS; TARGET PROTEINS; UNIFORM CELLS;

BATCH CELL CULTURE; CELL MEMBRANES; CELL PROLIFERATION; FLOW CYTOMETRY; FLUORESCENCE; METHANOL; PH EFFECTS; PROCESS ENGINEERING; PROTEINS; STRAIN;

CELL CULTURE;

FLUORESCENT DYE; METHANOL; RECOMBINANT PROTEIN;

DYE; ENZYME ACTIVITY; FLOW CYTOMETRY; METHANOL; PH; PHYSIOLOGY; PROTEIN; YEAST;

ARTICLE; BACTERIAL COUNT; BIOSYNTHESIS; BIOTECHNOLOGY; CELL PROLIFERATION; CULTURE MEDIUM; EVALUATION; FLOW CYTOMETRY; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; PHENOTYPE; PHYSIOLOGY; PICHIA;

BIOTECHNOLOGY; CELL PROLIFERATION; COLONY COUNT, MICROBIAL; CULTURE MEDIA; FLOW CYTOMETRY; FLUORESCENT DYES; METHANOL; PHENOTYPE; PICHIA; RECOMBINANT PROTEINS;

ARMORACIA RUSTICANA; PICHIA PASTORIS; SUS;

EID: 77954279954     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02475-09     Document Type: Article

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