Directed evolution to enhance secretion efficiency and thermostability of chitosanase from Mitsuaria chitosanitabida 3001

Bioscience, Biotechnology and Biochemistry

Volumn 70, Issue 2, 2006, Pages 559-563

  Yun, ChoongSoo ^a   Matsuda, Hideyuki ^a   Kawamukai, Makoto ^a  

^a SHIMANE UNIVERSITY   (Japan)

Author keywords

Chitosanase; Error prone PCR; Random mutagenesis; Thermostability

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; GENES; MUTAGENESIS; NITROGEN COMPOUNDS; THERMODYNAMIC STABILITY;

CHITOSANASE; ERROR-PRONE PCR; RANDOM MUTAGENESIS; THERMOSTABILITY;

ENZYMES;

CHITOSAN; CHITOSANASE; GLYCOSIDASE;

ARTICLE; BURKHOLDERIA; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; SECRETION; TEMPERATURE;

BURKHOLDERIA; CHITOSAN; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; GLYCOSIDE HYDROLASES; MUTATION; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 33344455273     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.70.559     Document Type: Article

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