Semi-rational approaches to engineering enzyme activity: Combining the benefits of directed evolution and rational design

Current Opinion in Biotechnology

Volumn 16, Issue 4, 2005, Pages 378-384

  Chica, Roberto A ^a   Doucet, Nicolas ^a   Pelletier, Joelle N ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME MODIFICATION; MUTATIONS;

ENZYMES; GENETIC ENGINEERING; MUTAGENESIS; SCAFFOLDS; SUBSTRATES;

ENZYME KINETICS;

AMINO ACID; AMINO ACID TRANSFER RNA LIGASE; ENZYME; EPOXIDE HYDROLASE;

CATALYSIS; ENZYME ACTIVITY; ENZYME MODIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE MUTATION; METHODOLOGY; MINERALIZATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RANDOMIZATION; REVIEW; RHIZOBIUM RADIOBACTER;

COMBINATORIAL CHEMISTRY TECHNIQUES; COMPUTATIONAL BIOLOGY; DIRECTED MOLECULAR EVOLUTION; ENZYMES; PROTEIN ENGINEERING;

EID: 23444450226     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.copbio.2005.06.004     Document Type: Review

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