Rational design of thermostable lactate oxidase by analyzing quaternary structure and prevention of deamidation

Biotechnology Letters

Volumn 27, Issue 22, 2005, Pages 1777-1784

  Kaneko, Hiroki ^a   Minagawa, Hirotaka ^b   Shimada, Jiro ^b  

^a NIHON UNIVERSITY   (Japan)

^b NEC CORPORATION   (Japan)

Author keywords

Biosensor; Computer aided design; Lactate oxidase; Protein engineering; Thermostability

Indexed keywords

BIOMEDICAL ENGINEERING; BIOSENSORS; CARBOXYLIC ACIDS; COMPUTER AIDED DESIGN; MATHEMATICAL MODELS; MOLECULAR STRUCTURE; PROTEINS;

DENATURATION; LACTATE OXIDASE; PROTEIN ENGINEERING; THERMOSTABILITY;

ENZYMES;

AMIDE; LACTATE 2 MONOOXYGENASE; LACTATE 2-MONOOXYGENASE; MIXED FUNCTION OXIDASE;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER AIDED DESIGN; COMPUTER SIMULATION; DRUG DESIGN; ENZYME STABILITY; ENZYMOLOGY; GENETIC ENHANCEMENT; GENETICS; METABOLISM; METHODOLOGY; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN QUATERNARY STRUCTURE; SITE DIRECTED MUTAGENESIS; STREPTOCOCCACEAE; STRUCTURE ACTIVITY RELATION;

AMIDES; AMINO ACID SUBSTITUTION; COMPUTER SIMULATION; COMPUTER-AIDED DESIGN; DRUG DESIGN; ENZYME STABILITY; GENETIC ENHANCEMENT; MIXED FUNCTION OXYGENASES; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN STRUCTURE, QUATERNARY; STREPTOCOCCACEAE; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 28344442808     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-005-3555-2     Document Type: Article

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