Directed evolution of an enantioselective Bacillus subtilis lipase

Biocatalysis and Biotransformation

Volumn 21, Issue 2 SPEC. ISS., 2003, Pages 67-73

  Funke, Susanne Aileen ^a   Eipper, Andreas ^b,d   Reetz, Manfred T ^b   Otte, Nikolaj ^b   Thiel, Walter ^b   Van Pouderoyen, Gertie ^c   Dijkstra, Bauke W ^c   Jaeger, Karl Erich ^a   Eggert, Thorsten ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

^d BASF SE   (Germany)

Author keywords

Bacillus subtilis; Directed evolution; Enantioselectivity; Esterase; Lipase; Saturation mutagenesis

Indexed keywords

CATALYST SELECTIVITY; ENZYME KINETICS; HYDROLYSIS; IONIZATION; MASS SPECTROMETRY; MOLECULAR DYNAMICS; MUTAGENESIS;

ENANTIOSELECTIVITY;

BIOCATALYSTS;

1,4 DIACETOXY 2 CYCLOPENTENE; ACID LIPASE; ALCOHOL DERIVATIVE; ESTERASE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

BACILLUS SUBTILIS; CHIRALITY; CONFERENCE PAPER; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME MECHANISM; ENZYME STRUCTURE; EVOLUTION; MASS SPECTROMETRY; MOLECULAR MECHANICS; MUTAGENESIS; NONHUMAN; PARALLEL DESIGN; PROTEIN HYDROLYSIS; QUANTUM MECHANICS; SIMULATION; STRUCTURE ANALYSIS; X RAY ANALYSIS;

BACILLUS SUBTILIS;

EID: 0041813141     PISSN: 10242422     EISSN: None     Source Type: Journal    
DOI: 10.1080/1024242031000110847     Document Type: Conference Paper

References (27)

1. Barettino, D., Feigenbutz, M., Valcárcel, R. and Stunnenberg, H.G. (1994) "Improved method for PCR-mediated site-directed mutagenesis", Nucleic Acids Res. 22, 541-542.
2. Bornscheuer, U.T. and Pohl, M. (2001) "Improved biocatalysts by directed evolution and rational protein design", Curr. Opin. Chem. Biol. 5, 137-143.
3. Eggert, T. and Jaeger, K.-E. (2003) "Directed evolution by random mutagenesis: A critical evaluation", In: Svendsen, A., ed, Enzyme Functionality: Design, Engineering and Screening (Marcel Dekker, New York) (in press).
4. Eggert, T., Pencreac'h, G., Douchet, I., Verger, R. and Jaeger, K.-E. (2000) "A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase", Eur. J. Biochem. 267, 6459 - 6469.
5. Funke, S.A., Eipper, A., Eggert, T., Jaeger, K.-E., Reetz, M.T. (submitted for publication).
6. Jaeger, K.-E. and Eggert, T. (2002) "Lipases for biotechnology", Curr. Opin. Biotechnol. 13, 390-397.
7. Jaeger, K.-E., Dijkstra, B.W. and Reetz, M.T. (1999) "Bacterial biocatalysts: Molecular biology, three-dimensional structures, and biotechnological applications of lipases", Annu. Rev. Microbiol. 53, 315-351.
8. Jaeger, K.-E., Eggert, T., Eipper, A. and Reetz, M.T. (2001) "Directed evolution and the creation of enantioselective biocatalysts", Appl. Microbiol. Biotechnol. 55, 519-530.
9. Liebeton, K., Zonta, A., Schimossek, K., Nardini, M., Lang, D., Dijkstra, B.W., Reetz, M.T. and Jaeger, K.-E. (2000) "Directed evolution of an enantioselective lipase", Chem. Biol. 7, 209-218.
10. Lutz, S., Ostermeier, M., Moore, G.I., Maranas, C.D. and Benkovic, S.J. (2001) "Creating multiple-crossover libraries independent of sequence identity", Proc. Natl. Acad. Sci. 98, 11248-11253.
11. Petrounia, I.P. and Arnold, F. (2000) "Designed evolution of enzymatic properties", Curr. Opin. Biotechnol. 11, 325 - 330.
12. Reetz, M.T. (2001) "Combinatorial and evolution-based methods in the creation of enantioselective catalysts", Angew. Chem. Int. Ed. 40, 284-310.
13. Reetz, M.T. (2002) "Lipases as practical biocatalysts", Curr. Opin. Chem. Biol. 6, 145-150.
14. Reetz, M.T. and Jaeger, K.-E. (2002) "Directed evolution as a means to create enantioselective enzymes for use in organic chemistry", In: Brakman, S. and Johnsson, K., eds, Directed Molecular Evolution of Proteins or How to Improve Enzymes for Biocatalysis (WILEY-VCH, Weinheim, Germany), pp 245-279.
15. Reetz, M.T., Zonta, A., Schimossek, K., Liebeton, K. and Jaeger, K.-E. (1997) "Creation of enantioselective biocatalysts for organic chemistry by in vitro evolution", Angew. Chem. Int. Ed. 36, 2830-2832.
16. Reetz, M.T., Becker, M.H., Klein, H.W. and Stöckigt, D. (1999) "A method for high-throughput screening of enantioselective catalysts", Angew. Chem. Int. Ed. 38, 1758-1761.
17. Rosenau, F. and Jaeger, K.-E. (2003) "Overexpression of biocatalysts in Pseudomonas", In: Svendsen, A., ed, Enzyme Functionality: Design, Engineering and Screening (Marcel Dekker, New York) (in press).
18. Rouhi, A.M. (2002) "Chiral roundup", Chem. Eng. News 80(23), 43-50.
19. Shao, Z., Zhao, H., Giver, L. and Arnold, F.H. (2000) "Random-priming in vitro recombination: An effective tool for directed evolution", Nucleic Acids Res. 26, 681-683.
20. Schöneboom, J.C., Lin, H., Reuter, N., Thiel, W., Cohen, S., Ogliaro, F. and Shaik, S. (2002) "The elusive oxidant species of cytochrome p450 enzymes: Characterization by combined quantum mechanical/molecular mechanical (QM/MM) calculations", J. Am. Chem. Soc. 124, 8142-8151.
21. Stemmer, W.P.C. (1994) "Rapid evolution of a protein in vitro by DNA shuffling", Nature 370, 389-391.
22. Stemmer, W.P.C. (1994) "DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution", Proc. Natl. Acad. Sci. 91, 10747-10751.
23. Stinson, S.C. (2001) "Chiral pharmaceuticals", Chem. Eng. News. 79(40), 79-97.
24. Van Pouderoyen, G., Eggert, T., Jaeger, K.-E. and Dijkstra, B.W. (2001) "The crystal structure of Bacillus subtilis lipase: A minimal α/β-hydrolase fold enzyme", J. Mol. Biol. 309, 215-226.
25. Zhao, H., Giver, L., Shao, Z., Affholter, J.A. and Arnold, F. (1998) "Molecular evolution by staggered extension process (StEP) in vitro recombination", Nat. Biotechnol. 16, 258-261.
26. Zhao, H., Chockalingam, K. and Chen, Z. (2002) "Directed evolution of enzymes and pathways for industrial biocatalysis", Curr. Opin. Biotechnol. 13, 104-110.
27. Zhou, Y.H., Zhang, X.P. and Ebright, R.H. (1991) "Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase", Nucleic Acids Res. 19, 6052.