Computational thermostabilization of an enzyme

Science

Volumn 308, Issue 5723, 2005, Pages 857-860

  Korkegian, Aaron ^a,b   Black, Margaret E ^c   Baker, David ^b   Stoddard, Barry L ^a  

^a Fred Hutchinson Cancer Research Center   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

^c WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOMEDICAL ENGINEERING; COMPUTATIONAL METHODS; MELTING; METABOLISM; MUTAGENESIS; THERMAL EFFECTS; THERMODYNAMIC STABILITY;

BACTERIAL GROWTH; COMPUTATIONAL THERMOSTABILIZATION; MELTING TEMPERATURE; METABOLIC ENGINEERING;

ENZYMES;

CYTOSINE DEAMINASE;

BIOCHEMISTRY; ENZYME; MEDICINE;

ARTICLE; BACTERIAL GROWTH; CATALYSIS; ENZYME STABILITY; ENZYME STRUCTURE; HALF LIFE TIME; MATHEMATICAL COMPUTING; MELTING POINT; MOLECULAR MODEL; MUTATION; PRIORITY JOURNAL; PROTEIN FOLDING; TEMPERATURE; THERMOSTABILITY;

AMINO ACID SEQUENCE; BINDING SITES; CATALYSIS; CIRCULAR DICHROISM; COMPUTER SIMULATION; CRYSTALLOGRAPHY, X-RAY; CYTOSINE DEAMINASE; ENZYME STABILITY; ESCHERICHIA COLI; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MONTE CARLO METHOD; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; SOFTWARE; TEMPERATURE; THERMODYNAMICS; TRANSFORMATION, GENETIC; YEASTS;

BACTERIA (MICROORGANISMS);

EID: 18244373419     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1107387     Document Type: Article

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38. The authors acknowledge the assistance and advice of the Baker Laboratory in running RosettaDesign, the FHCRC structural biology program for assistance with data collection, and critiques and suggestions from R. Strong and A. Ferre-D'Amare. Funding was provided by NIH grant nos. GM49857 and CA97328 (B.L.S.), CA97328 and CA85939 (M.E.B.), CM59224 (D.B.), and T32-GM08268 (A.K.). Crystal structures of the yCD double and triple mutants have been submitted to the Research Collaboratory for Structural Bioinformatics Protein Databank with accession codes 1YSD and 1YSB, respectively.