Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution

Protein Science

Volumn 7, Issue 3, 1998, Pages 698-705

  Akanuma, Satoshi ^a,b   Yamagishi, Akihiko ^a   Tanaka, Nobuo ^b   Oshima, Tairo ^a  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

3 isopropylmalate dehydrogenase; Evolutionary molecular engineering; Integration vector system; Thermal stability; Thermus thermophilus

Indexed keywords

3 ISOPROPYLMALATE DEHYDROGENASE; LEUCINE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; BACTERIUM TRANSFORMATION; DNA SEQUENCE; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; THERMOSTABILITY; THERMUS THERMOPHILUS;

BACILLUS SUBTILIS;

EID: 0031935580     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070319     Document Type: Article

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