Directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state

Protein Engineering, Design and Selection

Volumn 18, Issue 1, 2005, Pages 51-58

  Roodveldt, C ^a   Tawfik, D S ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

Apoenzyme; Directed evolution; Escherichia coli; Heterologous expression; Organophosphorus hydrolase; Phosphotriesterase; Promiscuity; Recombinant

Indexed keywords

ESCHERICHIA COLI; GENES; HYDROLYSIS; NEUROLOGY; PROTEINS;

METAL-FREE APOENZYME; NERVE AGENTS; PHOSPHOTRIESTERASE; PSEUDOMONAS DIMINUTA (PTE);

ENZYMES;

APOENZYME; ESTERASE; HOLOENZYME; METALLOPROTEINASE; ORGANOPHOSPHORUS COMPOUND; PHOSPHOTRIESTERASE; ZINC DERIVATIVE;

ARTICLE; BREVUNDIMONAS DIMINUTA; DIRECTED MOLECULAR EVOLUTION; ENZYME DENATURATION; ESCHERICHIA COLI; GENE LIBRARY; HYDROLYSIS; METAL BINDING; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PRODUCT RECOVERY; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN STABILITY; SENSITIVITY ANALYSIS; THERMAL ANALYSIS;

CHELATING AGENTS; DIRECTED MOLECULAR EVOLUTION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; PHOSPHORIC TRIESTER HYDROLASES; PROTEIN DENATURATION; PSEUDOMONAS; RECOMBINANT FUSION PROTEINS;

BREVUNDIMONAS DIMINUTA; ESCHERICHIA COLI; PSEUDOMONAS;

EID: 17144364646     PISSN: 17410126     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/gzi005     Document Type: Article

References (45)

1. Aharoni, A., Gaidukov, L., Yagur, S., Toker, L., Silman, I. and Tawfik, D.S. (2004) Proc. Natl Acad. Sci. USA, 101, 482-487.
2. Aharoni, A., Gaidukov, L., Khersonsky, O., McQ Gould, S., Roodveldt, C. and Tawfik, D.S. (2005) Nat. Genet., 37, 73-77.
3. Arnold, F.H., Wintrode, P.L., Miyazaki, K. and Gershenson, A. (2001) Trends Biochem. Sci., 26, 100-106.
4. Benning, M.M., Kuo, J.M., Raushel, F.M. and Hazel, H.M. (1994) Biochemistry, 33, 15001-15007.
5. Benning, M.M., Kuo, J.M., Raushel, F.M. and Holden, H.M. (1995) Biochemistry, 34, 7973-7978.
6. Caldwell, S.R. and Raushel, F.M. (1991) Appl. Biochem. Biotechnol., 31, 59-73.
7. Chen-Goodspeed, M., Sogorb, M.A., Wu, F., Hong, S.B. and Raushel, F.M. (2001a) Biochemistry, 40, 1325-1331.
8. Chen-Goodspeed, M., Sogorb, M.A., Wu, F. and Raushel, F.M. (2001b) Biochemistry, 40, 1332-1339.
9. Cho, C.M., Mulchandani, A. and Chen, W. (2004) Appl. Environ. Microbiol., 70, 4681-4685.
10. Cho, C.M., Mulchandani, A. and Chen, W. (2002) Appl. Environ. Microbiol., 68, 2026-2030.
11. Dave, K.I., Miller, C.E. and Wild, J.R. (1993) Chem. Biol. Interact., 87, 55-48.
12. DiSioudi, B., Grimsley, J.K., Lai, K. and Wild, J.R. (1999) Biochemistry, 38, 2866-2872.
13. Dumas, D.P., Caldwell, S.R., Wild, J. and Raushel, F.M. (1989) J. Biol. Chem., 264, 19659-16665.
14. Esteban, O. and Zhao, H. (2004) J. Mol. Biol., 340, 81-95.
15. Gill, I. and Ballesteros, A. (2000) Biotechnol. Bioeng., 70, 400-410.
16. Griffiths, A. and Tawfik, D.S. (2003). EMBO J., 22, 24-35.
17. Grimsley, J.K., Scholtz, J.M., Pace, C.N. and Wild, J.R. (1997) Biochemistry, 36, 14366-14374.
18. Hantke, K. (2001) Biometals, 14, 239-249.
19. Hong, S.B. and Raushel, F.M. (1999) Biochemistry, 38, 1159-1165.
20. Hoskin, F.C.G., Walker, J.E., Dettbarn, W.D. and Wild, J.R. (1995) Biochem. Pharmacol., 49, 711-715.
21. James, L.C. and Tawfik, D.S. (2001) Protein Sci., 10, 2600-2607.
22. Khalameyzer, V., Fischer, I., Bornscheuer, U. and Altenbuchner J. (1999) Appl. Environ. Microbiol., 65, 477-482.
23. Kuo, J.M. and Raushel, F.M. (1994) Biochemistry, 33, 4265-4272.
24. Lai, K., Dave, K.I. and Wild, J.R. (1994) J. Biol. Chem., 269, 16579-16584.
25. LeJune, K.E., Wild, J.R. and Russell, A.J. (1998) Nature, 395, 27-28.
26. Morawski, B., Quan, S. and Arnold, F.H. (2001) Biotechnol. Bioeng., 76, 99-107.
27. O'Brien, P.J. and Herschlag, D. (1999) Chem. Biol., 6, R91-R105.
28. Omburo, G.A., Kuo, J.M., Mullins, L.S. and Raushel, F.M. (1992) J. Biol. Chem., 267, 13278-13283.
29. Outten, C.E. and O'Halloran, T.V. (2001) Science, 292, 2488-2492.
30. Rastogi, V.K., DeFrank, J.J., Cheng, T.C. and Wild, J.R. (1997) Biochem. Biophys. Res. Commun., 241, 294-296.
31. Raushel, F.M. and Holden, H.M. (2000) Adv. Enzymol. Relat. Areas Mol. Biol., 74, 51-93.
32. Remaud-Simeon, M. (2004) FEBS Lett., 560, 91-97.
33. Richins, R.D., Kaneva, I., Mulchandani, A. and Chen, W. (1997) Nat. Biotechnol., 15, 984-987.
34. Rochu, D., Viguie, N., Renault, F., Crouzier, D., Froment, M.T. and Masson, P. (2004). Biochem. J., 380, 627-633.
35. Roodveldt, C, Aharoni, A. and Tawfik, D.S. (2005) Curr. Opin. Struct. Biol., 15, 50-56.
36. Shim, H., Hong, S.B. and Raushel, F.M. (1998) J. Biol. Chem., 273, 17445-17450.
37. Shimazu, M., Mulchandani, A. and Chen, W. (2001). Biotechnol. Prog., 17, 76-80.
38. Shimazu, M., Nguyen, A., Mulchandani, A. and Chen, W. (2003) Biotechnol. Prog., 19, 1612-1614.
39. Shusta, E.V., Holler, P.D., Kieke, M.C., Kranz, D.M. and Wittrup, K.D. (2000) Nat. Biotechnol., 18, 754-759.
40. Stemmer, W.P. (1994). Nature. 370, 389-391.
41. Turner, N.J. (2003) Trends Biotecnol., 21, 474-478.
42. Vanhooke J.L., Benning, M.M., Raushel, F.M. and Holden, H.M. (1996) Biochemistry, 35, 6020-6025.
43. Vartanian, J.P., Henry, M. and Wain-Hobson, S. (1996) Nucleic Acids Res., 24, 2627-2631.
44. Yang, H., Carr, P.D., McLoughlin, S.Y., Liu, J.W., Home, I., Qiu, X., Jeffries, C.M., Russell, R.J., Oakeshott, J.G. and Ollis, D.L. (2003) Protein Eng., 16, 135-145.
45. Zaccolo, M., Williams, D.M., Brown, D.M. and Gherardi, E. (1996) J. Mol. Biol., 255, 589-603.