Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration

Applied and Environmental Microbiology

Volumn 71, Issue 10, 2005, Pages 5728-5734

  Johannes, Tyler W ^a   Woodyer, Ryan D ^a   Zhao, Huimin ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOCATALYSTS; CATALYSIS; COMPLEXATION; MUTAGENESIS; PHOSPHATES; SYNTHESIS (CHEMICAL); THERMODYNAMIC STABILITY;

BIOCATALYSIS; CATALYTIC EFFICIENCY; THERMOSTABILIZATION; THERMOSTABLE PHOSPHITE DEHYDROGENASE;

ENZYME KINETICS;

FORMATE DEHYDROGENASE; LEUCINE; MUTANT PROTEIN; OXIDOREDUCTASE; PHOSPHITE; PHOSPHITE DEHYDROGENASE; PYRUVIC ACID DERIVATIVE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRIMETHYLPYRUVATE; UNCLASSIFIED DRUG;

MICROBIOLOGY;

AMINO ACID SUBSTITUTION; ARTICLE; BIOCATALYST; BIOTRANSFORMATION; CANDIDA BOIDINII; CATALYSIS; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME STABILITY; ESCHERICHIA COLI; HALF LIFE TIME; HIGH THROUGHPUT SCREENING; MOLECULAR EVOLUTION; MOLECULAR MODEL; MUTAGENESIS; NONHUMAN; PSEUDOMONAS STUTZERI; SITE DIRECTED MUTAGENESIS; TEMPERATURE; THERMOSTABILITY;

AMINO ACID SUBSTITUTION; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; HEAT; INDUSTRIAL MICROBIOLOGY; MODELS, MOLECULAR; NADH, NADPH OXIDOREDUCTASES; NADP; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; PSEUDOMONAS STUTZERI;

CANDIDA BOIDINII; PSEUDOMONAS STUTZERI;

EID: 26844523510     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.10.5728-5734.2005     Document Type: Article

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