Improvement in thermal stability and substrate binding of pig kidney D-amino acid oxidase by chemical modification

Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

Volumn 112, Issue 3, 2004, Pages 123-131

  Bakke, Mikio ^a   Kajiyama, Naoki ^a  

^a KIKKOMAN CORPORATION   (Japan)

Author keywords

Chemical modification; D amino acid oxidase; Km value; Soluble dextran; Stabilization

Indexed keywords

AMINO ACIDS; CHARACTERIZATION; ENZYMES; MODIFICATION; OPTIMIZATION; ORGANIC POLYMERS;

LIQUID ASSAY REAGENT; PIG KIDNEY D-AMINO ACID OXIDASE (PKDAAO); SUBSTRATE BINDING;

BIOLOGICAL ORGANS;

ACETIC ACID; ALANINE; BUFFER; DEXTRAN; DEXTRO ALANINE; DEXTRO AMINO ACID OXIDASE; DEXTRO SERINE; GLYCINE; METHIONINE; POTASSIUM; PROLINE; PYRIDINE; REAGENT; TRYPTOPHAN; VALINE; AMINO ACID; FLAVINE ADENINE NUCLEOTIDE;

ARTICLE; CENTRIFUGATION; CHEMICAL MODIFICATION; CONJUGATE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; GEL PERMEATION CHROMATOGRAPHY; KIDNEY; NONHUMAN; PH; REACTION TIME; SWINE; THERMOSTABILITY; ANIMAL; CHEMISTRY; ENZYMOLOGY; GEL CHROMATOGRAPHY; HEAT; KINETICS; METABOLISM; OXIDATION REDUCTION REACTION; PROTEIN BINDING; TEMPERATURE;

SUS SCROFA;

AMINO ACIDS; ANIMALS; CHROMATOGRAPHY, GEL; D-AMINO-ACID OXIDASE; DEXTRANS; ENZYME STABILITY; FLAVIN-ADENINE DINUCLEOTIDE; HEAT; HYDROGEN-ION CONCENTRATION; KIDNEY; KINETICS; OXIDATION-REDUCTION; PROTEIN BINDING; SUBSTRATE SPECIFICITY; SWINE; TEMPERATURE;

EID: 11244341067     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1385/ABAB:112:3:123     Document Type: Article

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