Increased Rigidity of Domain Structures Enhances the Stability of a Mutant Enzyme Created by Directed Evolution

Biochemistry

Volumn 42, Issue 49, 2003, Pages 14469-14475

  Hoseki, Jun ^a   Okamoto, Akihiro ^a   Takada, Naoki ^b   Suenaga, Atsushi ^b   Futatsugi, Noriyuki ^b   Konagaya, Akihiko ^b   Taiji, Makoto ^b   Yano, Takato ^a   Kuramitsu, Seiki ^c,d   Kagamiyama, Hiroyuki ^a  

^a OSAKA MEDICAL COLLEGE   (Japan)

^b RIKEN YOKOHAMA INSTITUTE   (Japan)

^c OSAKA UNIVERSITY   (Japan)

^d RIKEN HARIMA INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; COMPUTER SIMULATION; MOLECULAR DYNAMICS; RIGIDITY; THERMODYNAMIC STABILITY;

MUTATIONS;

ENZYMES;

CYSTEINE; KANAMYCIN NUCLEOTIDYLTRANSFERASE; MUTANT PROTEIN; NUCLEOTIDYLTRANSFERASE; REAGENT; THIOL GROUP; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME STABILITY; ENZYME STRUCTURE; GENE MUTATION; MOLECULAR DYNAMICS; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; SIMULATION; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; TECHNIQUE; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

ALANINE; AMINO ACID SUBSTITUTION; AMINO ACIDS; BACTERIAL PROTEINS; CYSTEINE; DIRECTED MOLECULAR EVOLUTION; DITHIONITROBENZOIC ACID; ENZYME STABILITY; HEAT; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; NUCLEOTIDYLTRANSFERASES; PROTEIN STRUCTURE, TERTIARY; SULFHYDRYL REAGENTS; THERMODYNAMICS;

EID: 10744233518     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi034776z     Document Type: Article

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