Emerging options in protein bioseparation

Biotechnology Annual Review

Volumn 12, Issue , 2006, Pages 1-29

  Mondal, Kalyani ^a   Jain, Sulakshana ^a   Teotia, Sunita ^a   Gupta, Munishwar N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; ALGINIC ACID; ALKALINE PROTEINASE; AVIDIN; BETA GLUCOSIDASE; CARRAGEENAN; CELLULOSE; CHITINASE; CHITOSAN; CHYMOTRYPSIN; CONCANAVALIN A; EUDRAGIT; GALACTOMANNAN; HYDROXYPROPYLMETHYLCELLULOSE ACETATE SUCCINATE; LACTATE DEHYDROGENASE; LECTIN; LYSOZYME; MONOCLONAL ANTIBODY; ORGANIC SOLVENT; POLY(N ISOPROPYLACRYLAMIDE); POLYACRYLAMIDE; POLYGALACTURONASE; PROTEIN A; PYRUVATE KINASE; RECOMBINANT DNA; SODIUM CHLORIDE; TRYPSIN; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; WHEAT GERM AGGLUTININ;

AFFINITY CHROMATOGRAPHY; CAPILLARY ELECTROPHORESIS; CELL SEPARATION; CHROMATOGRAPHY; CRYSTALLIZATION; DOWNSTREAM PROCESSING; ELECTROPHORESIS; ENZYME ANALYSIS; ENZYMOLOGY; EXPANDED BED CHROMATOGRAPHY; GEL FILTRATION CHROMATOGRAPHY; HEATING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INCUBATION TIME; INTERMETHOD COMPARISON; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC POINT; MASS SPECTROMETRY; MATHEMATICAL ANALYSIS; PARTITION COEFFICIENT; PHASE PARTITIONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEOMICS; RECOMBINANT DNA TECHNOLOGY; REVIEW; THERMOSTABILITY; TISSUE CULTURE;

BIOTECHNOLOGY; CHROMATOGRAPHY; CHROMATOGRAPHY, AFFINITY; POLYMERS; PRECIPITATION; PROTEINS; SALTS; SOLVENTS;

EID: 33749453802     PISSN: 13872656     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S1387-2656(06)12001-3     Document Type: Review

References (171)

1. Sumner J.B. The isolation and crystallization of the enzyme urease. Preliminary paper. J Biol Chem 69 (1926) 435-441
2. Northrop J.H. Crystallization of pepsin from alcohol. J Gen Physiol 30 (1946) 177-184
3. Kunitz M. Isolation of a crystalline compound of trypsin and of soybean trypsin inhibitor. J Gen Physiol 30 (1947) 311-320
4. Fruton JS and Simmonds S (eds). General properties of protein. In: General Biochemistry, 2nd edn, New York, Wiley, 1958, pp. 14-44.
5. Dixon M and Webb EC (eds). Enzyme isolation. In: Enzymes, UK, Longmans Green & Co. Ltd, 1958, pp. 27-53.
6. Peterson E.A., and Sober H.A. Chromatography of proteins. I. Cellulose ion exchange adsorbents. J Am Chem Soc 78 (1956) 751-755
7. Porath J., and Flodin P. Gel filtration: a method for desalting and group separation. Nature 183 (1959) 1657-1659
8. Axén R., Porath J., and Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogens halides. Nature 214 (1967) 1302-1304
9. In: Godfrey T., and West S. (Eds). Industrial Enzymology (1988), London, Macmillan Press Ltd.
10. Smyth DG. Techniques in enzymic hydrolysis. In: Methods in Enzymology, Vol. 11, New York, Academic Press, 1967, pp. 214-231.
11. Rovery M. Chromatography of trypsin to remove chymotrypsin and of chymotrypsin to remove trypsin. In: Methods in Enzymology, Vol. 11, New York, Academic Press, 1967, pp. 231-236.
12. Ryu D.D.Y., and Nam D.H. Biomolecular engineering: a new frontier in biotechnology. J Mol Catal B: Enzym 10 (2000) 23-37
13. Sunic D., McNeil J.D., Andress D.L., and Belford D.A. Insulin-like growth factor binding protein-5 proteolytic activity in ovine articular chondrocyte culture. Biochim Biophys Acta-Gen Subjects 1425 (1998) 567-576
14. Jaeger K.E. Protein technologies and commercial enzyme. White is the hype. Biocatalyst on the move. Curr Opin Biotechnol 15 (2004) 269-271
15. Jenck J.F., Agterberg F., and Droescher M.J. Products and processes for a sustainable chemical industry: a review of achievments and prospects. Green Chem 6 (2004) 544-556
16. In: Means G.E., and Feeny R.E. (Eds). Chemical Modification of Proteins (1971), Holden Day Inc, London
17. Tyagi R., and Gupta M.N. Chemical modification and chemical crosslinking for protein/enzyme stabilization. Biochemistry (Moscow) 63 (1998) 334-344
18. In: Taylor R.F. (Ed). Protein Immobilization (1991), Marcel Dekker Inc, New York
19. Trower M.K. (1996), Humana Press Inc, Totowa, NJ
20. Turner N.J. Directed evolution of enzymes for applied biocatalysis. Trends Biotechnol 21 (2003) 474-478
21. Labrou N.E. Directed enzyme evolution: bridging the gap between natural enzymes and commercial applications. Biomol Eng 22 (2005) vii-ix
22. Morley K.L., and Kazlauskas R.J. Improving enzyme properties: when are closer mutations better? Trends Biotechnol 23 (2005) 231-237
23. Gupta M.N. Enzyme function in organic solvents. Eur J Biochem 203 (1992) 25-32
24. Gupta M.N., and Roy I. Enzymes in organic media: forms, function and applications. Eur J Biochem 271 (2004) 2575-2583
25. In: Gupta M.N. (Ed). Methods in Non-aqueous Enzymology (2000), Birkhauser Verlag, Basel, Switzerland
26. Lichty J.J., Malecki J.L., Agnew H.D., Michelson-Horowitz D.J., and Tan S. Comparison of affinity tags for protein purification. Protein Expression Purif 41 (2005) 98-105
27. Lesley S.A. High-throughput proteomics: protein expression and purification in the postgenomic world. Protein Expression Purif 22 (2001) 159-164
28. Smith C. Striving for purity: advances in protein purification. Nat Methods 2 (2005) 71-77
29. Bajpai S., Sharma A., and Gupta M.N. Removal and recovery of antinutritional factors from soybean flour. Food Chem 89 (2005) 497-501
30. Jain S., and Gupta M.N. An integrated process for separation of major and minor proteins from goat serum. Appl Biochem Biotechnol 125 (2005) 53-62
31. Harsa S., and Furusaki S. Chromatographic separation of amyloglucosidase from the mixtures of enzymes. Biochem Eng J 8 (2001) 257-261
32. Sharma A., and Gupta M.N. Macroaffinity ligand facilitated three-phase partitioning (MLFTPP) for purification of xylanase. Biotechnol Bioeng 80 (2002) 228-232
33. Bonnerjea J., Oh S., Hoare M., and Dunnill P. Protein purification: the right step at the right time. Bio/Technology 4 (1986) 954-958
34. Kaul R.H., and Mattiasson B. Secondary purification. Bioseparation 3 (1992) 1-26
35. LaVallie E.R., and McCoy J.M. Gene fusion expression systems in E. coli. Curr Opin Biotechnol 6 (1995) 501-506
36. Sharma A., Sharma S., and Gupta M.N. Purification of wheat germ amylase by precipitation. Protein Expression Purif 18 (2000) 111-114
37. Teotia S., Lata R., Khare S.K., and Gupta M.N. One step purification of glucoamylase by affinity precipitation with alginate. J Mol Recogn 14 (2001) 295-299
38. Schuster M., Wasserbauer E., Ortner C., Graumann K., Jungbauer A., Hammerschmid F., and Werner G. Short cut of protein purification by integration of cell-disrupture and affinity extraction. Bioseparation 9 (2000) 59-67
39. Pierpoint WS. The extraction of enzymes from plant tissues rich in phenolic compounds. In: Methods in Molecular Biology, Doonan S (ed), Protein Purification Protocols, Totowa, NJ, Humana Press Inc, 1996, pp. 58-68.
40. Chase H.A. Purification of proteins by adsorption chromatography in expanded beds. Trends Biotechnol 12 (1994) 296-303
41. Anspach F.B., Curbelo D., Hartmann R., Garke G., and Deckwer W.D. Expanded bed chromatography in primary protein purification. J Chromatogr A 865 (1999) 129-144
42. Jensen L.B., Riise E., Nielsen L.K., Dziegiel M., Fugger L., and Engberg J. Efficient purification of unique antibodies using peptide affinity-matrix columns. J Immunol Methods 284 (2004) 45-54
43. Jungbauer A. Chromatographic media for bioseparation. J Chromatogr A 1065 (2005) 3-12
44. Cuatrecasas P., Wilchek M., and Anfinsen C.B. Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci 61 (1968) 636-643
45. Thompson S.T., Cass K.H., and Stellwagen E. Blue Dextran-Sepharose: an affinity column for the dinucleotide fold in proteins. Proc Natl Acad Sci 72 (1975) 669-672
46. Stellwagen E. Use of blue dextran as a probe for the nicotinamide adenine dinucleotide domain in proteins. Acc Chem Res 10 (1977) 92-98
47. Denizli A., and Piskin E. Dye-ligand affinity systems. J Biochem Biophys Methods 49 (2001) 391-416
48. Porath J., Carlsson J., Olsson I., and Belfrage G. Metal chelate affinity chromatography: a new approach to protein fractionation. Nature 258 (1975) 598-599
49. Huang P.Y., Baumbach G.A., Dadd C.A., Buettner J.A., Masecar B.L., Hentsch M., Hammond D.J., and Carbonell R.G. Affinity purification of von Willebrand factor using ligands derived from peptide libraries. Bioorg Med Chem 4 (1996) 699-708
50. Chen B., Bestetti G., Day R.M., and Turner A.P.F. The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin. Biosensor Bioelectron 13 (1998) 779-785
51. Romig T.S., Bell C., and Drolet D.W. Aptamer affinity chromatography: combinatorial chemistry applied to protein purification. J Chromatogr B, Biomed Sci Appl 731 (1999) 275-284
52. Larsson P.O., and Mosbach K. Affinity precipitation of enzymes. FEBS Lett 98 (1979) 333-338
53. Burnett T.J., Peebles H.C., and Hageman J.H. Synthesis of a fluorescent boronic acid which reversibly binds to cell walls and a diboronic acid which agglutinates erythrocytes. Biochem Biophys Res Commun 96 (1980) 157-162
54. Hoffmann A.S. Bioconjugates of intelligent polymers and recognition proteins for use in diagnostics and affinity separations. Clin Chem 46 (2000) 1478-1486
55. Roy I., and Gupta M.N. Smart polymeric materials: emerging biochemical applications. Chem Biol 10 (2003) 1161-1171
56. Linne E., Garg N., Kaul R.H., and Mattiasson B. Evaluation of alginate as a ligand carrier. Biotechnol Appl Biochem 16 (1992) 48
57. Schneider M., Guillot C., and Lamy B. The affinity precipitation technique. Application of isolation and purification of trypsin from bovine pancreas. Ann N Y Acad Sci USA 369 (1981) 257-263
58. Pécs M., Eggert M., and Schügerl K. Affinity precipitation of extracellular microbial enzymes. J Biotechnol 21 (1991) 137-142
59. Morris J.E., Hoffman A.S., and Fisher R.R. Affinity precipitation of proteins by polyligands. Biotechnol Bioeng 41 (1993) 991-997
60. Maeda M., Nishimura C., Inenaga A., and Takagi M. 1993 Modification of DNA with poly(N-isopropylacrylamide) for thermally innduced affinity separation. Reactive Polym 21 (1995) 27-35
61. Taniguchi M., Kobayashi M., Natsui K., and Fujii M. Purification of Staphylococcal protein A by affinity precipitation using a reversible soluble-insoluble polymer with human IgG as a ligand. J Ferment Bioeng 68 (1989) 32-36
62. Guoquiang D., Lali A., Kaul R.H., and Mattiasson B. Affinity thermoprecipitation of lactate dehydrogenase and pyruvate kinase from porcine muscle using Eudragit bound cibacron blue. J Biotechnol 37 (1994) 23-31
63. Guoquiang D., Benhura M.A.N., Kaul R.H., and Mattiasson B. Affinity thermoprecipitation of yeast alcohol-dehydrogenase through metal ion-promoted binding with Eudragit-bound cibacron-blue-3GA. Biotechnol Prog 11 (1995) 187-193
64. Kumar A., and Gupta M.N. Affinity precipitation of trypsin with soybean trypsin inhibitor linked Eudragit S-100. J Biotechnol 37 (1994) 185-189
65. Larsson E.L., and Mattiasson B. Isolation of Con A by affinity precipitation. Biotechnol Tech 8 (1994) 51-56
66. Taipa M.A., Kaul R., Mattiasson B., and Cabral J.M.S. Preliminary studies on the purification of a monoclonal antibody by affinity precipitation with Eudragit S-100. J Mol Recogn 11 (1998) 240-242
67. Guoquiang D., Kaul R.H., and Mattiasson B. Purification of Lactobacillus bulgaricus d-lactate dehydrogenase by precipitation with an anionic polymer. Bioseparation 3 (1993) 333-341
68. Gupta M.N., Guoquiang D., Kaul R.H., and Mattiasson B. Purification of xylanase from Trichoderma viride by precipitation with an anionic polymer Eudragit S-100. Biotechnol Techn 8 (1993) 117-122
69. Breccia J.D., Siñeriz F., Baigorí M.D., Castro G.R., and Kaul R.H. Purification and characterization of a thermostable xylanase from Bacillus amyloliquefaciens. Enzyme Microb Technol 22 (1998) 42-49
70. Sun Y., Yu K., Jin X.H., and Zhou X.Z. Polymerized liposome as ligand carrier for affinity precipitation of proteins. Biotechnol Bioeng 47 (1995) 20-25
71. Senstad C., and Mattiasson B. Purification of wheat germ agglutinin using affinity flocculation with chitosan and a subsequent centrifugation or flotation step. Biotechnol Bioeng 34 (1989) 387-393
72. Homma T., Fujii M., Mori J., Kawakami T., Kuroda K., and Taniguchi M. Production of cellobiose by enzymatic hydrolysis: removal of β-glucosidase from cellulase by affinity precipitation using chitosan. Biotechnol Bioeng 41 (1993) 405-410
73. Tyagi R., Kumar A., Sardar M., Kumar S., and Gupta M.N. Chitosan as an affinity macroligand for precipitation of N-acetyl glucosamine binding proteins/enzymes. Iso Purif 2 (1996) 217-226
74. Teotia S., Lata R., and Gupta M.N. Chitosan as a macroaffinity ligand: purification of chitinases by affinity precipitation and aqueous two-phase extractions. J Chromatogr A 1052 (2004) 85-91
75. Gupta M.N., Guoquiang D., and Mattiasson B. Purification of endopolygalacturonase by affinity precipitation using alginate. Biotechnol Appl Biochem 18 (1993) 321-327
76. Teotia S., Khare S.K., and Gupta M.N. An efficient purification process for sweet potato β-amylase by affinity precipitation with alginate. Enzyme Microb Technol 28 (2001) 792-795
77. Sharma S., Sharma A., and Gupta M.N. 2000 One step purification of peanut phospholipase D by precipitation with alginate. Bioseparation 9 (2001) 93-98
78. Bradshaw A.P., and Sturgeon R.J. The synthesis of soluble polymer-ligand complexes for affinity precipitation studies. Biotechnol Techn 4 (1990) 67-71
79. Mondal K., Roy I., and Gupta M.N. κ-Carrageenan as a carrier in affinity precipitation of yeast alcohol dehydrogenase. Protein Expression Purif 32 (2003) 151-160
80. Walter H., and Johansson G. Partitioning in aqueous two-phase systems: an overview. Anal Biochem 155 (1986) 215-242
81. Albertsson PA (ed). Partition of Cell Particles and Macromolecules, 3rd edn, New York, Wiley (Interscience), 1986.
82. Rito-Palomares M. Practical application of aqueous two phase partition to process development for the recovery of biological products. J Chromatogr B 807 (2004) 3-11
83. Walker S.J., Dale C.J., and Lyddiatt A. Aqueous two-phase partition of complex protein feedstocks derived from brain tissue homogenates. J Chromatogr B 680 (1996) 91-96
84. Harris D.P., Andrews A.T., Wright G., Pyle D.L., and Asenjo J.A. The application of aqueous two-phase systems to the purification of pharmaceutical proteins from transgenic sheep milk. Bioseparation 7 (1997) 31-37
85. Alves J.G.L.F., Chumpitaz L.D.A., da Silva L.H.M., Franco T.T., and Meirelles A.J.A. Partitioning of whey proteins, bovine serum albumin and porcine insulin in aqueous two-phase systems. J Chromatogr B 743 (2000) 235-240
86. Benavided J., and Rito-Palomares M. Bioprocess intensification: a potential aqueous two phase process for the primary recovery of B-phycoerythrin from Porphyridium Cruentum. J Chromatogr B 807 (2004) 33-38
87. Jain S., Teotia S., and Gupta M.N. Purification of green fluorescent protein overexpressed by a mutant recombinant E. coli. Protein Expression Purif 36 (2004) 76-81
88. Lu M., Tjerneld F., Johansson G., and Albertsson P.A. Preparation of benzoyl-dextran and its use in aqueous two-phase systems. Bioseparation 2 (1991) 247-255
89. Stewart R.M., and Todd P. A poly(ethylene glycol)-sodium chloride multiphase system for extraction of acid hydrolasates. In: Todd P., Sikdar S.K., and Bier M. (Eds). Frontiers in Bioprocessing II (1992), American Chemical Society, Washington, DC 352-356
90. Snyder S.M., Cole K.D., and Szlag C.C. Phase composition viscosities, and densities for aqueous two phase system composed of polyethylene glycol and various salts at 25 °C. J Chem Eng Data 37 (1992) 268-274
91. Pietruszka N., Galaev I.Y., Kumar A., Brzozowski Z.K., and Mattiasson B. 2000 New polymers forming aqueous two phase polymer systems. Biotechnol Prog 16 (1996) 408-415
92. Oliverira L.A., Neto B.B., Porto A.L.F., and Tambourg E.B. Extractive cultivation of xylanase by Penicillin janthinellum in a poly(ethylene glycol)/cashew nut gum aqueous two phase system. Biotechnol Prog 20 (2004) 1880-1884
93. Johansson G. Effects of salts on the partition of proteins in aqueous polymeric biphasic systems. Acta Chem Scand Sec B 28 (1974) 873-882
94. Bamberger S., Seaman G.V.F., Brown J.A., and Brooks D.V. The partition of sodium phosphate and sodium chloride in aqueous dextran poly(ethylene glycol) two phase systems. J Colloid Inter Sci 99 (1984) 187-193
95. Teotia S., and Gupta M.N. Reversibly soluble macroaffinity ligand in aqueous two-phase separation of enzymes. J Chromatogr A 923 (2001) 275-280
96. In: Hatti-Kaul R. (Ed). Aqueous Two Phase Systems: Methods and Protocols (2000), Humana Press Inc, Totowa, NJ
97. Cordes A., and Kula M.-R. Process design for large-scale purification of formate dehydrogenase from Candida boidinii by affinity partitioning. J Chromatogr 376 (1986) 375-384
98. Kamihara M., Kaul R., and Mattiasson B. Purification of recombinant protein A by aqueous two phase extraction integrated with affinity precipitation. Biotechnol Bioeng 40 (1992) 1381-1387
99. Teotia S., Lata R., and Gupta M.N. Free polymeric bioligands in aqueous two-phase affinity extractions of microbial xylanases and pullulanase. Protein Expression Purif 22 (2001) 484-488
100. Teotia S., and Gupta M.N. Purification of phospholipase D by two-phase affinity extraction. J Chromatogr A 1025 (2004) 297-301
101. Albertsson P.A., Johansson G., and Tjerneld F. Aqueous two phase separations. In: Asenjo J.A. (Ed). Separation Processes in Biotechnology (1990), Marcel Dekker, New York 287-327
102. Veide A., Lindbäck T., and Enfors S.O. Continuous extraction of β-d-galactosidase from E. coli in an aqueous two-phase system: effects of biomass concentration on partitioning and mass transfer. Enzyme Microb Technol 6 (1984) 325-330
103. Boland M.J., Hesselink P.G.M., Papamichael N., and Hustedt H. Extractive purification of enzymes from animal tissue using aqueous two phase systems: pilot scale studies. J Biotechnol 19 (1991) 19-34
104. Paulsen J., and Hustedt H. Extractive purification of aspartase from E. coli K12. In: Walter H., and Johansson G. (Eds). Methods in Enzymology Vol. 228 (1994), Academic Press, New York 590-599
105. Li M., and Peeples T.L. Purification of hyperthermophilic archael amylotytic enzyme (MJAI) using thermoseparating aqueous two phase systems. J Chromatgr B 807 (2004) 69-74
106. Johansson G., and Andersson M. Parameters determining affinity partitioning of yeast enzymes using polymer-bound triazine dye ligands. J Chromatogr 303 (1984) 39-51
107. Johansson G., and Kopperschlager G. Effects of organic solvents on the partitioning of enzymes in aqueous two-phase systems. J Chromatogr 388 (1987) 295-305
108. Wuenschell G.E., Naranjo E., and Arnold F.H. Aqueous two-phase metal affinity extraction of heme proteins. Bioprocess Eng 5 (1990) 199-202
109. Luther J.R., and Glatz C.E. Genetically engineered charge modifications to enhance protein separation in aqueous two phase systems: electrochemical partitioning. Biotechnol Bioeng 44 (1994) 147-153
110. Luther J.R., and Glatz C.E. Genetically engineered charge modifications to enhance protein separation in aqueous two phase systems: charge directed partitioning. Biotechnol Bioeng 46 (1995) 62-68
111. Bandmann N., Collet E., Leijen J., Uhlén M., Veide A., and Nygren P.-A. Genetic engineering of the Fusarium solani pisi lipase cutinase for enhanced partitioning in PEG-phosphate aqueous two-phase systems. J. Biotechnol 79 (2000) 161-172
112. Fexby S., and Bulow L. Improved partitioning in aqueous two phase system of tyrosine-tagged recombinant lactate dehydrogenase. Protein Expression Purif 25 (2002) 263-269
113. Gouveia T.D., and Kilikian B.V. Bioaffinity extraction of glucoamylase in aqueous two phase systems using starch as free bioligand. J Chromatogr B 743 (2000) 241-246
114. Cisneros M., Benavides J., Brenes C., and Rito-Palomares M. Recovery in aqueous two phase systems of lutein produced by the green microalga Chlorella protothecoides. J Chromatogr A 807 (2004) 105-110
115. Wei D.-Z., Zhu J.-H., and Cao X.-J. Enzymatic synthesis of cephalexin in aqueous two phase systems. Biochem Eng J 11 (2002) 95-99
116. Kuboi R., Morita S., Ota H., and Umakoshi H. Protein refolding using stimuli-responsive polymer-modified aqueous two-phase systems J Chromatogr B 743 (2000) 215-223
117. Bermudez O., and Forciniti D. Aggregation and denaturation of antibodies: a capillary electrophoresis, dynamic light scattering, and aqueous two phase partitioning study. J Chromatgr B 807 (2004) 17-24
118. Walker S.J., and Lyddiatt A. Aqueous two-phase systems as an alternative process route for the fractionation of small inclusion bodies. J Chromatogr B 711 (1998) 185
119. Sharma S., Teotia S., and Gupta M.N. Bioconversion in an aqueous two phase system using a smart biocatalyst: casein hydrolysis by alpha chymotrypsin. Enzyme Microb Technol 32 (2003) 337-339
120. Dennison C., and Lovrien R. Three phase partitioning: concentration and purification of proteins. Protein Expression Purif 11 (1997) 149-161
121. In: Scopes R.K. (Ed). Protein Purification: Principles and Practice (1987), Springer, New York pp. 71-101
122. Hatzinikolaou D.G., Tsoukia C., Kekos D., and Macris B.J. An efficient and optimized purification procedure for the superoxide dismutase from Aspergillus niger. Partial characterization of the purified enzyme. Bioseparation 7 (1997) 39-46
123. Morton R.K. Separation and purification of enzymes associated with insoluble particles. Nature 166 (1950) 1092-1095
124. Melius P., and Simmons W.S. Utilization of 1-butanol for the preparation of pancreatic lipase. Biochim Biophys Acta 105 (1965) 600-602
125. Pike R.N., and Dennison C. Protein fractionation by three phase partitioning (TPP) in aqueous/t-butanol mixtures. Biotechnol Bioeng 33 (1989) 221-228
126. Borbas R., Turza S., Szamos J., and Kiss E. Analysis of protein gels formed by interfacial partitioning. Colloid Poly Sci 279 (2001) 705-713
127. Kiss E., Szamos J., Tamas B., and Borbas R. Interfacial behaviour of proteins in three phase partitioning using salt-containing water/tert-butanol systems. Colloids Surf A 142 (1998) 295-302
128. Lovrien R., Goldensoph C., Anderson P.C., and Odegaard B. Three phase partitioning (TPP) via t-butanol: enzymes separation from crudes. In: Burgess R. (Ed). Protein Purification: Micro to Macro (1987), Alan R. Liss Inc, New York, NY 131-148
129. Singh R.K., Gourinath S., Sharma S., Roy I., Gupta M.N., Betzel C., Srinivasan A., and Singh T.P. Enhancement of enzyme activity through three phase partitioning: crystal structure of a modified serine proteinase at 1.5 Å resolution. Protein Eng 14 (2001) 307-313
130. Roy I., and Gupta M.N. α-Chymotrypsin shows higher activity in water as well as organic solvents after three phase partitioning. Biocatal Biotransform 22 (2004) 261-268
131. Singh N., Jabeen T., Sharma S., Roy I., Gupta M.N., Bilgrami S., Somvanshi R.K., Dey S., Perbandt M., Betzel C., and Singh T.P. Detection of native peptides as potent inhibitors of enzymes: crystal structure of the complex formed between the treated bovine α-chymotrypsin and an autocatalytically produced fragment Ile-Val-Asn-Gly-Glu-Glu-Ala-Val-Pro-Gly-Ser-Trp-Pro-Trp at 2.2 Å resolution. FEBS J 272 (2005) 562-572
132. Jain S., Singh R., and Gupta M.N. Purification of recombinant green fluorescent protein by three-phase partitioning. J Chromatogr A 1035 (2004) 83-86
133. Sharma S., and Gupta M.N. Purification of phospholipase D from Dacus carota by three phase partitioning and its characterization. Protein Expression Purif 21 (2001) 310-316
134. Sharma A., Sharma S., and Gupta M.N. Purification of alkaline phosphatase from chicken intestine by three phase partitioning and use of phenyl-Sepharose 6B in the batch mode. Bioseparation 9 (2000) 155-161
135. Sharma A., and Gupta M.N. Purification of pectinases by three phase partitioning. Biotechnol Lett 23 (2001) 1625-1627
136. Roy I., and Gupta M.N. Three phase affinity partitioning of proteins. Anal Biochem 300 (2002) 11-14
137. Sharma A., and Gupta M.N. Three phase partitioning of carbohydrate polymers - separation and purification of alginates. Carb Polym 48 (2002) 391-395
138. Sharma A., Mondal K., and Gupta M.N. Some studies on characterization of three phase partitioned chitosan. Carb Polym 52 (2003) 433-438
139. Mondal K., Sharma A., and Gupta M.N. Macroaffinity ligand facilitated three phase partitioning (MLFTPP) of α-amylases using modified alginate. Biotechnol Prog 19 (2003) 493-494
140. Mondal K., Sharma A., and Gupta M.N. Macroaffinity ligand facilitated three phase partitioning (MLFTPP) for purification of glucoamylase and pullulanase using alginate. Protein Expression Purif 28 (2003) 190-195
141. Sharma A., Mondal K., and Gupta M.N. Separation of enzymes by sequential macroaffinity ligand-facilitated three-phase partitioning. J Chromatogr A 995 (2003) 127-134
142. Borbas R., Murray B.S., and Kiss E. Interfacial shear rheological behaviour of proteins in three phase partitioning systems. Colloid Surf A 213 (2003) 93-103
143. Roy I., Sharma A., and Gupta M.N. Three phase partitioning for simultaneous renaturation and partial purification of Aspergillus niger xylanase. Biochim Biophys Acta 1698 (2004) 107-110
144. Sharma A., Khare S.K., and Gupta M.N. Three phase partitioning of extraction of oil from soybean. Bioresource Technol 85 (2002) 327-329
145. Hjorth R., Leijon P., Frej A.K.B., and Jagersten C. Expanded bed adsorption chromatography. In: Subramanian G. (Ed). Bioseparation & Bioprocessing Vol. 1 (1998), Wiley, Weinheim 199-226
146. Chase H.A. Prediction of the performance of preparative affinity chromatography. J Chromatogr 297 (1984) 179-202
147. Barnfield F.A.K., Hjorth R., and Hammarstrom A. Pilot scale recovery of recombinant annexin from unclarified E. coli homogenate using expanded bed adsorption. Biotechnol Bioeng 44 (1994) 922-929
148. Chase H.A., and Draeger N.M. Affinity purification of proteins using expanded beds. J Chromatogr 597 (1992) 129-145
149. Clemmitt R.H., and Chase H.A. Immobilised metal affinity chromatography of β-galactosidase from unclarified E. coli homogenates using expanded bed adsorption. J Chromatogr A 874 (2000) 27-43
150. Choe W., Clemmit R.H., Chase H.A., and Middelberg A.P.J. Comparison of histidine tag capture chemistries for purification following chemical extraction. J Chromatogr A 953 (2002) 111-121
151. Roy I., and Gupta M.N. Purificatioon of a 'double headed' inhibitor of alpha-amylase/proteinase K from wheat germ by expanded bed chromatography. Bioseparation 9 (2000) 239-245
152. Clemmitt R.H., and Chase H.A. Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded bed absorption. Biotechnol Bioeng 67 (2000) 206-216
153. Thömmes J., Bader A., Halfar M., Karau A., and Kula M.R. Isolation of monoclonal antibodies from cell containing hybridoma broth using a protein A coated adsorbent in expanded beds. J Chromatogr A 752 (1996) 111-122
154. TM Red H-E7B. Adv Bioprocess Eng 1994;519-525.
155. McCreath G.E., Chase H.A., Purvis D.R., and Lowe C.R. Novel affinity separations based on perfluorocarbon emulsions. Use of perfluorocarbon affinity emulsion for the purification of human serum albumin from blood plasma in a fluidized bed. J Chromatogr A 597 (1992) 189-196
156. Cocker T.M., Fee C.J., and Evans R.A. Preparation of magnetically susceptible polyacrylamide/magnetite beads for use in magnetically stabilized fluid bed chromatography. Biotechnol Bioeng 53 (1997) 79-87
157. Roy I., Pai A., Lali A., and Gupta M.N. Comparison of batch, packed bed and expanded bed purification of A. niger cellulase using cellulose beads. Bioseparation 8 (1999) 317-326
158. Somers W., van't Riet K., Rozie H., Rombouts F., and Visser J. Isolation and purification of endopolygalacturonase by affinity chromatography in a fluidized bed reactor. Chem Eng J 40 (1989) 137-139
159. Roy I., Sardar M., and Gupta M.N. Exploiting unusual affinity of usual polysaccharides for bioseparation of enzymes on fluidized beds. Enzyme Microb Technol 27 (2000) 53-65
160. Sharma S., Roy I., and Gupta M.N. Separation of phospholipase D from peanut on a fluidized bed of crosslinked alginate beads. Biochem Eng J 8 (2001) 235-239
161. Roy I., and Gupta M.N. Purification of a bacterial pullulanase on a fluidized bed of calcium alginate beads. J Chromatogr A 950 (2002) 131-137
162. Roy I., Jain S., Teotia S., and Gupta M.N. Evaluation of micro beads of calcium alginate for affinity chromatography of Aspergillus niger pectinase. Biotechnol Prog 20 (2004) 1490-1495
163. Tong X., Dong X., and Sun Y. Lysozyme adsorption and purification by expanded bed chromatography with a small sized dense adsorbent. Biochem Eng J 12 (2002) 117-124
164. Hidayat C., Takagi M.M., and Yoshida T. Expanded bed adsorption for purification of alcohol dehydrogenase using iminodiacetic acid matrix. J Biosci Bioeng 97 (2004) 284-287
165. Haynes C. The importance of bioseparations: giving credit where credit is due. Biotechnol Bioeng 87 (2004) 257-258
166. Przybycien T.M., Pujar N.S., and Steele L.M. Alternative bioseparation operations: life beyond packed bed chromatography. Curr Opin Biotechnol 15 (2004) 469-478
167. D'Souza S.F., and Deshpande A. Simultaneous purification and reversible immobilization of d-amino acid oxidase from Trigonopsis variabilis on a hydrophobic support. Appl Biochem Biotechnol 5 (2001) 83-92
168. Sardar M., Roy I., and Gupta M.N. Simultaneous purification and immobilization of Aspergillus niger xylanase on the reversibly soluble polymer Eudragit™ L-100. Enzyme Microb Technol 27 (2000) 672-679
169. Melo J.S., and D'Souza S.F. A simple approach for the simultaneous isolation and immobilization of invertase using crude extracts of yeast and Jack bean meal. J Biochem Biophys Methods 16 (2000) 133-135
170. Schoevaart R., Wolbers M.W., Golubovic M., Ottens M., Kieboom A.P.G., Van Rantwijk F., van der Wielen L.A.M., and Sheldon R.A. Preparation, optimization and structures of cross-linked enzyme aggregates (CLEAs). Biotechnol Bioeng 87 (2004) 754-762
171. Sharma S., and Gupta M.N. Alginate as a macroaffinity ligand and an additive for enhanced activity and thermostability of lipases. Biotechnol Appl Biochem 35 (2001) 161-165