High-throughput proteomics: Protein expression and purification in the postgenomic world

Protein Expression and Purification

Volumn 22, Issue 2, 2001, Pages 159-164

  Lesley, Scott A ^a  

^a GENOMICS INSTITUTE OF THE NOVARTIS RESEARCH FOUNDATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DRUG MANUFACTURE; GENE PRODUCT; PLANT BIOTECHNOLOGY; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

EID: 0034957669     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1465     Document Type: Review

References (12)

1. The sequence of the human genome
2. Initial sequencing and analysis of the human genome
3. The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes
4. DNA cloning using in vivo site-specific recombination
5. Metal-chelate affinity chromatography
6. Ribonuclease S-peptide as a carrier in fusion proteins
7. A single step purification for recombinant proteins
8. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
9. Histidine patch thioredoxins. Mutant forms of thioredoxin with metal chelating affinity that provide for convenient purifications of thioredoxin fusion proteins
10. Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins
11. An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein
12. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element