Short cut of protein purification by integration of cell-disrupture and affinity extraction

Bioseparation

Volumn 9, Issue 2, 2000, Pages 59-67

  Schuster, M ^a,b   Wasserbauer, E ^a   Ortner, C ^b   Graumann, K ^b   Jungbauer, A ^b   Hammerschmid, F ^a   Werner, G ^a  

^a Brunnerstrasse 59   (Austria)

^b UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

Author keywords

Affinity chromatography; Cell disruption; FLAG peptide; Magnetic glass beads; Yeast

Indexed keywords

AFFINITY CHROMATOGRAPHY; CELL DISRUPTION; CHELATING AGENT; COMPLEMENTARY DNA; GENE OVEREXPRESSION; GENE PRODUCT; HUMAN SERUM ALBUMIN; HYBRID PROTEIN; IMMOBILIZED ANTIBODY; MONOCLONAL ANTIBODY; MOUSE; PROTEIN DETECTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; SUSPENSION CELL CULTURE;

ACETIC ACID; AMINO ACIDS; CELL IMMOBILIZATION; CHELATION; CHEMICAL BONDS; DISINTEGRATION; EXTRACTION; PURIFICATION; YEAST;

CELL DISINTEGRATION; CYTOPLASM; PEPTIDE;

MONOCLONAL ANTIBODIES;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; CELL FRACTIONATION; CHROMATOGRAPHY, AFFINITY; HUMANS; IMMUNOMAGNETIC SEPARATION; MICE; PEPTIDES; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SERUM ALBUMIN;

EID: 0034042651     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008135913202     Document Type: Article

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