Separation of phospholipase D from peanut on a fluidized bed of crosslinked alginate beads

Biochemical Engineering Journal

Volumn 8, Issue 3, 2001, Pages 235-239

  Sharma, Shweta ^a   Roy, Ipsita ^a   Gupta, Munishwar N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Alginate; Fluidized beds; Phospholipase D

Indexed keywords

ALGINIC ACID; EPICHLOROHYDRIN; GULURONIC ACID; MANNURONIC ACID; PHOSPHOLIPASE D; POLYSACCHARIDE; UNCLASSIFIED DRUG;

ARTICLE; CROSS LINKING; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME PURIFICATION; FLUIDIZED BED; NONHUMAN; PEANUT; PRECIPITATION; PRIORITY JOURNAL; RESIDUE ANALYSIS; STREPTOMYCES;

ARACHIS HYPOGAEA;

EID: 0034755469     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1369-703X(01)00113-9     Document Type: Article

References (27)

1. Exploiting unusual affinity of usual polysaccharides for separation of enzymes on fluidized beds
2. Expanded bed chromatography in primary protein purification
3. Expanded bed protein A affinity of a recombinant humanized monoclonal antibody: Process development, operation, and comparison with a packed bed method
4. Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded-bed adsorption
5. Expanded bed adsorption: Elution in expanded bed mode
6. Use of streamline chelating for capture and purification of poly-His-tagged recombinant proteins
7. Expanded bed adsorption in industrial bioprocessing: Recent developments
8. Comparison of batch, packed bed and expanded bed purification of A. Niger cellulase using cellulose beads
9. One step purification of peanut phospholipase D by precipitation with alginate
10. Alginate as immobilisation matrix for cells
11. Measurement of phospholipase D activity
12. Identification and characterisation of a novel plant phospholipase D that requires polyphosphoinoditides and submicromolar calcium for activity in Arabidopsis
13. Improved purification and biochemical characterisation of phospholipase D from cabbage
14. Phospholipase D from soyabean (Glycine max L.) suspension-cultured cells: Purification, structural and enzymatic properties
15. Purification and properties of two phospholipase D from Streptomyces sp.
16. Phospholipase D: Enzymology, mechanism of regulation and function
17. Using phospholipases for phospholipid modification
18. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
19. Phospholipase D from peanut seeds. IV. Final purification and some properties of the enzyme
20. Alginate beads as an affinity material for alpha amylases
21. Purification of α-amylase using magnetic alginate beads
22. Isolation and purification of endo-polygalactouronase by affinity chromatography in a fluidized bed reactor
23. Prediction of the performance of preparative affinity chromatography
24. Purification of xylanase from Trichoderma viride by precipitation with an anionic polymer Eudragit S-100
25. Affinity precipitation using chitosan as a ligand carrier
26. Purification of endopolygalactouronase by affinity precipitation using alginate