Free polymeric bioligands in aqueous two-phase affinity extractions of microbial xylanases and pullulanase

Protein Expression and Purification

Volumn 22, Issue 3, 2001, Pages 484-488

  Teotia, Sunita ^a   Lata, R ^b   Gupta, M N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b INDIAN AGRICULTURAL RESEARCH INSTITUTE   (India)

Author keywords

Alginate; Aqueous two phase system; Eudragit S 100; Polyethylene glycol (PEG); Pullulanase; Reversibly soluble insoluble polymers; Xylanase

Indexed keywords

ASPERGILLUS NIGER; BACILLUS ACIDOPULLULYTICUS; BACILLUS AMYLOLIQUEFACIENS; ENZYME ACTIVITY; ENZYME PURIFICATION; EXTRACTION; MACROGOL; MICROBIAL ENZYME; PROTEIN FOLDING; PULLULANASE; TRICHODERMA REESEI; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ASPERGILLUS NIGER; BACILLUS ACIDOPULLULYTICUS; BACILLUS AMYLOLIQUEFACIENS; HYPOCREA JECORINA; TRICHODERMA;

EID: 0034888428     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1466     Document Type: Article

References (28)

1. Aqueous two phase protein partitioning using textile dyes as affinity ligands
2. Purification of recombinant protein A by aqueous two phase extraction integrated with affinity precipitation
3. Integration of aqueous two phase extraction and affinity precipitation for the purification of lactate dehydrogenase
4. Affinity cell separations: Problems and prospects
5. Effect of specific binding reactions on the partitioning behavior of biomaterials
6. Bioaffinity extraction of glucoamylase in aqueous two-phase systems using starch as free bioligand
7. Aqueous two phase partitioning
8. Purification of xylanase from Trichoderrna viride by precipitation with an anionic polymer Eudragit S-100
9. Non-covalent immobilization of enzymes on an enteric polymer, Eudragit S-100
10. Preparation and characterization and application of Aspergillus sp. xylanase immobilized on Eudragit S-100
11. Simultaneous purification and immobilization of Aspergillus niger xylanase on the reversibly soluble polymer Eudragit L-100
12. Alginate as immobilization matrix for cells
13. Purification of wheat germ amylase by precipitation
14. Exploiting unusual affinity of usual polysaccharides for separation of enzymes on fluidized beds
15. Purification of α-amylase isoenzymes from Scytalidium thermophilum on a fluidized bed of alginate beads followed by concanavalin A-agarose column chromatography
16. Purification of endo-polygalacturonase by affinity precipitation using alginate
17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
18. Interlaboratory testing of methods for assay of xylanase activity
19. Amylases α and β
20. The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis
21. Glycopeptides
22. An introduction to polyacrylamide gel electrophoresis
23. Fractionation and purification of endo-1,4-beta-xylanases and exo-1,4-beta-xylosidases of Aspergillus niger
24. The two major xylanases from Trichoderma reesei: Characterization of both enzymes and genes
25. Microbial xylanases for paper industry
26. Microbial amylolytic enzymes
27. β-1,4-D-xylandegrading enzyme systems: Biochemistry, molecular biology and applications
28. Starch conversion