-
2
-
-
4744358151
-
-
A method for growing enzyme crystals. US Patent 5120650; 1992.
-
Cultor Ltd. (Visuri K): A method for growing enzyme crystals. US Patent 5120650; 1992.
-
-
-
Visuri, K.1
-
3
-
-
4744340085
-
-
Subtilisin crystallization process. US Patent 5041377; 1991.
-
Genencor International Inc. (Becker NT, Lawlis VB Jr): Subtilisin crystallization process. US Patent 5041377; 1991.
-
-
-
Becker, N.T.1
Lawlis Jr., V.B.2
-
4
-
-
4744342612
-
-
Method for rapidly obtaining crystals with desireable morphologies. US Patent 6593118; 2003. in press.
-
Genencor International Inc. (Heng M): Method for rapidly obtaining crystals with desireable morphologies. US Patent 6593118; 2003.. in press. Illustrates the importance of temperature to simultaneously increase kinetics of industrial crystallization processes and obtain morphologies of crystals that improve process yields.
-
-
-
Heng, M.1
-
5
-
-
0012002711
-
Economics of antibody production
-
J. Morrow Economics of antibody production Genet Eng News 22 2002 1 39
-
(2002)
Genet Eng News
, vol.22
, pp. 1-39
-
-
Morrow, J.1
-
6
-
-
4744337810
-
Perilous economics of the industry
-
Sept/Oct/Nov
-
Rankin P: Perilous economics of the industry. The Source 2003, Sept/Oct/Nov: 5-10.
-
(2003)
The Source
, pp. 5-10
-
-
Rankin, P.1
-
7
-
-
0038173662
-
Purification of capsular polysaccharide from Streptococcus pneumoniae serotype 23F by a procedure suitable for scale-up
-
V.M.M. Gonçalves, M. Takagi, R.B. Lima, H. Massaldi, R.C. Giordano, and M.M. Tanizaki Purification of capsular polysaccharide from Streptococcus pneumoniae serotype 23F by a procedure suitable for scale-up Biotechnol Appl Biochem 37 2003 283 287
-
(2003)
Biotechnol Appl Biochem
, vol.37
, pp. 283-287
-
-
Gonçalves, V.M.M.1
Takagi, M.2
Lima, R.B.3
Massaldi, H.4
Giordano, R.C.5
Tanizaki, M.M.6
-
8
-
-
0037200989
-
Fractional precipitation of plasmid DNA from lysate by CTAB
-
R.J. Lander, M.A. Winters, F.J. Meacle, B.C. Buckland, and A.L. Lee Fractional precipitation of plasmid DNA from lysate by CTAB Biotechnol Bioeng 79 2002 776 784 One of the first industrial applications of a non-chromatographic process for the purification of plasmid DNA.
-
(2002)
Biotechnol Bioeng
, vol.79
, pp. 776-784
-
-
Lander, R.J.1
Winters, M.A.2
Meacle, F.J.3
Buckland, B.C.4
Lee, A.L.5
-
9
-
-
0037420336
-
DNA purification by triple-helix affinity precipitation
-
M.D. Costioli, I. Fisch, F. Garret-Flaudy, F. Hilbrig, and R. Freitag DNA purification by triple-helix affinity precipitation Biotechnol Bioeng 81 2003 535 545
-
(2003)
Biotechnol Bioeng
, vol.81
, pp. 535-545
-
-
Costioli, M.D.1
Fisch, I.2
Garret-Flaudy, F.3
Hilbrig, F.4
Freitag, R.5
-
10
-
-
0842321815
-
Affinity purification of plasmid DNA by temperature-triggered precipitation
-
J. Kostal, A. Mulchandani, and W. Chen Affinity purification of plasmid DNA by temperature-triggered precipitation Biotechnol Bioeng 85 2004 293 297 ELPs are conjugated with a DNA-binding protein resulting in a thermally reversible affinity precipitation system specific for plasmids that incorporate the corresponding DNA-binding sequence. The possibilities for using protein engineering to tailor the response of this system are seemingly endless.
-
(2004)
Biotechnol Bioeng
, vol.85
, pp. 293-297
-
-
Kostal, J.1
Mulchandani, A.2
Chen, W.3
-
11
-
-
0012774928
-
Precipitation of RNA impurities with high salt in a plasmid DNA purification process: Use of experimental design to determine reaction conditions
-
A. Eon-Duval, K. Gumbs, and C. Ellett Precipitation of RNA impurities with high salt in a plasmid DNA purification process: use of experimental design to determine reaction conditions Biotechnol Bioeng 83 2003 544 553
-
(2003)
Biotechnol Bioeng
, vol.83
, pp. 544-553
-
-
Eon-Duval, A.1
Gumbs, K.2
Ellett, C.3
-
12
-
-
0036326352
-
Large scale production and downstream processing of a recombinant porcine parvovirus vaccine
-
L. Maranga, P. Rueda, A.F.G. Antonis, C. Vela, J.P.M. Langeveld, J.I. Casal, and M.J.T. Carrondo Large scale production and downstream processing of a recombinant porcine parvovirus vaccine Appl Micro Biotechnol 59 2002 45 50
-
(2002)
Appl Micro Biotechnol
, vol.59
, pp. 45-50
-
-
Maranga, L.1
Rueda, P.2
Antonis, A.F.G.3
Vela, C.4
Langeveld, J.P.M.5
Casal, J.I.6
Carrondo, M.J.T.7
-
13
-
-
2642572488
-
Purification of hyperthermophilic archaeal amylolytic enzyme (MJA1) using thermoseparating aqueous two-phase systems
-
M. Li, and T.L. Peeples Purification of hyperthermophilic archaeal amylolytic enzyme (MJA1) using thermoseparating aqueous two-phase systems J Chromatogr B 807 2004 69 74
-
(2004)
J Chromatogr B
, vol.807
, pp. 69-74
-
-
Li, M.1
Peeples, T.L.2
-
14
-
-
0038119852
-
Pilot-scale extraction of an intracellular recombinant cutinase from E. coli cell homogenate using a thermoseparating aqueous two-phase system
-
C. Kepka, E. Collet, J. Persson, A. Stahl, T. Lagerstedt, F. Tjerneld, and A. Veide Pilot-scale extraction of an intracellular recombinant cutinase from E. coli cell homogenate using a thermoseparating aqueous two-phase system J Biotechnol 103 2003 165 181
-
(2003)
J Biotechnol
, vol.103
, pp. 165-181
-
-
Kepka, C.1
Collet, E.2
Persson, J.3
Stahl, A.4
Lagerstedt, T.5
Tjerneld, F.6
Veide, A.7
-
15
-
-
1842763756
-
Protein partitioning in aqueous two-phase systems composed of a pH-responsive copolymer and poly(ethylene glycol)
-
S.M. Waziri, B.F. Abu-Sharkh, and S.A. Ali Protein partitioning in aqueous two-phase systems composed of a pH-responsive copolymer and poly(ethylene glycol) Biotechnol Prog 20 2004 526 532
-
(2004)
Biotechnol Prog
, vol.20
, pp. 526-532
-
-
Waziri, S.M.1
Abu-Sharkh, B.F.2
Ali, S.A.3
-
16
-
-
0037141601
-
Primary recovery of a genetically engineered Trichoderma reesei endoglucanase I (Cel 7B) fusion protein in cloud point extraction systems
-
A. Collen, K. Selber, T. Hyytia, J. Persson, T. Nakari-Setla, M. Bailey, R. Fagerstrom, M.-R. Kula, M. Penttila, and H. Stalbrand Primary recovery of a genetically engineered Trichoderma reesei endoglucanase I (Cel 7B) fusion protein in cloud point extraction systems Biotechnol Bioeng 78 2002 385 394
-
(2002)
Biotechnol Bioeng
, vol.78
, pp. 385-394
-
-
Collen, A.1
Selber, K.2
Hyytia, T.3
Persson, J.4
Nakari-Setla, T.5
Bailey, M.6
Fagerstrom, R.7
Kula, M.-R.8
Penttila, M.9
Stalbrand, H.10
-
17
-
-
0037039743
-
Cutinase-peptide fusions in thermoseparating aqueous two-phase systems. Prediction of partitioning and enhanced tag efficiency by detergent addition
-
A. Nilsson, M. Mannesse, M.R. Egmond, and F. Tjerneld Cutinase-peptide fusions in thermoseparating aqueous two-phase systems. Prediction of partitioning and enhanced tag efficiency by detergent addition J Chromatogr A 946 2002 141 155 The differences in hydrophobicities between the phases in an EOPO/starch system are enhanced by adding detergents to the system, resulting in improved fusion protein partitioning to the EOPO phase.
-
(2002)
J Chromatogr a
, vol.946
, pp. 141-155
-
-
Nilsson, A.1
Mannesse, M.2
Egmond, M.R.3
Tjerneld, F.4
-
18
-
-
0037121457
-
Partitioning of peptide-tagged proteins in aqueous two-phase systems using hydrophobically modified micelle-forming thermoseparating polymer
-
A. Nilsson, H.-O. Johansson, M. Mannesse, M.R. Egmond, and F. Tjerneld Partitioning of peptide-tagged proteins in aqueous two-phase systems using hydrophobically modified micelle-forming thermoseparating polymer Biochim Biophys Acta 1601 2002 138 148 The Tjerneld group has pioneered the use of hydrophobic peptide tags to bias partitioning in ATPE systems, producing many nice papers. This paper describes a careful study of tryptophan tag and spacer effects on protein partitioning in hydrophobically modified EOPO/starch systems. Tag exposure is the key to good partitioning.
-
(2002)
Biochim Biophys Acta
, vol.1601
, pp. 138-148
-
-
Nilsson, A.1
Johansson, H.-O.2
Mannesse, M.3
Egmond, M.R.4
Tjerneld, F.5
-
19
-
-
0036421610
-
Improved partitioning in aqueous two-phase system of tyrosine-tagged recombinant lactate dehydrogenase
-
S. Fexby, and L. Bulow Improved partitioning in aqueous two-phase system of tyrosine-tagged recombinant lactate dehydrogenase Prot Exp Purif 25 2002 263 269 A holistic approach to hydrophobic peptide tag development. Tyrosine doesn't partition as strongly as tryptophan in EOPO/dextran systems, but there may be a net advantage considering the relative expression levels and thermal stabilities of tyrosine-containing and tryptophan-containing fusion proteins.
-
(2002)
Prot Exp Purif
, vol.25
, pp. 263-269
-
-
Fexby, S.1
Bulow, L.2
-
20
-
-
2942704125
-
Partitioning and characterization of tyrosine-tagged green fluorescent proteins in aqueous two-phase systems
-
S. Fexby, A. Nilsson, G. Hambraeus, F. Tjerneld, and L. Buelow Partitioning and characterization of tyrosine-tagged green fluorescent proteins in aqueous two-phase systems Biotechnol Prog 20 2004 793 798
-
(2004)
Biotechnol Prog
, vol.20
, pp. 793-798
-
-
Fexby, S.1
Nilsson, A.2
Hambraeus, G.3
Tjerneld, F.4
Buelow, L.5
-
21
-
-
0037059484
-
Extraction of endoglucanase I (Cel7B) fusion proteins from Trichoderma reesei culture filtrate in a poly(ethylene glycol)-phosphate aqueous two-phase system
-
A. Collen, M. Penttila, H. Stalbrand, F. Tjerneld, and A. Veide Extraction of endoglucanase I (Cel7B) fusion proteins from Trichoderma reesei culture filtrate in a poly(ethylene glycol)-phosphate aqueous two-phase system J Chromatogr A 943 2002 55 62
-
(2002)
J Chromatogr a
, vol.943
, pp. 55-62
-
-
Collen, A.1
Penttila, M.2
Stalbrand, H.3
Tjerneld, F.4
Veide, A.5
-
22
-
-
0037081755
-
A novel two-step extraction method with detergent/polymer systems for primary recovery of the fusion protein endoglucanase I-hydrophobin I
-
A. Collen, J. Persson, M. Linder, T. Nakari-Setala, M. Penttila, F. Tjerneld, and U. Sivars A novel two-step extraction method with detergent/polymer systems for primary recovery of the fusion protein endoglucanase I-hydrophobin I Biochim Biophys Acta 1569 2002 139 150
-
(2002)
Biochim Biophys Acta
, vol.1569
, pp. 139-150
-
-
Collen, A.1
Persson, J.2
Linder, M.3
Nakari-Setala, T.4
Penttila, M.5
Tjerneld, F.6
Sivars, U.7
-
23
-
-
0037117912
-
Fundamental investigation of protein partitioning in two-phase aqueous mixed (nonionic/ionic) micellar systems
-
D.T. Kamei, D.I.C. Wang, and D. Blankschtein Fundamental investigation of protein partitioning in two-phase aqueous mixed (nonionic/ionic) micellar systems Langmuir 18 2002 3047 3057
-
(2002)
Langmuir
, vol.18
, pp. 3047-3057
-
-
Kamei, D.T.1
Wang, D.I.C.2
Blankschtein, D.3
-
24
-
-
0037457697
-
Glucose-6-phosphate dehydrogenase partitioning in two-phase aqueous mixed (nonionic/cationic) micellar systems
-
C.O. Rangel-Yagui, H. Lam, D.T. Kamei, D.I.C. Wang, A. Pessoa Jr., and D. Blankschtein Glucose-6-phosphate dehydrogenase partitioning in two-phase aqueous mixed (nonionic/cationic) micellar systems Biotechnol Bioeng 82 2003 445 456
-
(2003)
Biotechnol Bioeng
, vol.82
, pp. 445-456
-
-
Rangel-Yagui, C.O.1
Lam, H.2
Kamei, D.T.3
Wang, D.I.C.4
Pessoa Jr., A.5
Blankschtein, D.6
-
25
-
-
0035552564
-
Physical properties of detergent-based aqueous two-phase systems
-
K. Selber, S. Muller, H. Gieren, J. Thommes, T. Sottmann, R. Strey, and M.-R. Kula Physical properties of detergent-based aqueous two-phase systems Bioseparation 10 2002 243 253
-
(2002)
Bioseparation
, vol.10
, pp. 243-253
-
-
Selber, K.1
Muller, S.2
Gieren, H.3
Thommes, J.4
Sottmann, T.5
Strey, R.6
Kula, M.-R.7
-
26
-
-
0037706852
-
Technological aspects of extractive fermentation using aqueous two-phase systems
-
R.M. Banik, A. Santhiagu, B. Kanari, C. Sabarinath, and S.N. Upadhyay Technological aspects of extractive fermentation using aqueous two-phase systems World J Microbiol Biotechnol 19 2003 337 348
-
(2003)
World J Microbiol Biotechnol
, vol.19
, pp. 337-348
-
-
Banik, R.M.1
Santhiagu, A.2
Kanari, B.3
Sabarinath, C.4
Upadhyay, S.N.5
-
27
-
-
0035553798
-
Acoustic field assisted demixing of aqueous two-phase polymer systems
-
N.D. Srinivas, N. Nagaraj, and K.S.M.S. Raghavarao Acoustic field assisted demixing of aqueous two-phase polymer systems Bioseparation 10 2002 203 210
-
(2002)
Bioseparation
, vol.10
, pp. 203-210
-
-
Srinivas, N.D.1
Nagaraj, N.2
Raghavarao, K.S.M.S.3
-
28
-
-
0346459923
-
Affinity two-phase partitioning in acoustically levitated drops
-
S. Santesson, I.B-.R. Ramirez, P. Viberg, B. Jergil, and S. Nilsson Affinity two-phase partitioning in acoustically levitated drops Anal Chem 76 2004 303 308 This paper describes a system in which the two-phase partitioning of biotinylated liposomes in a PEG/avidin-modified dextran system within individual ultrasonically levitated droplets, ranging in volume from 0.1 to 2 μL, was monitored. Will this be the ultimate ATPE process development tool of the future or is it too impractical? Regardless, it's just plain cool.
-
(2004)
Anal Chem
, vol.76
, pp. 303-308
-
-
Santesson, S.1
Ramirez, I.B.-R.2
Viberg, P.3
Jergil, B.4
Nilsson, S.5
-
29
-
-
0344665699
-
Relationship between the protein surface hydrophobicity and its partitioning behaviour in aqueous two-phase systems of polyethyleneglycol- dextran
-
G. Tubio, B. Nerli, and G. Pico Relationship between the protein surface hydrophobicity and its partitioning behaviour in aqueous two-phase systems of polyethyleneglycol-dextran J Chromatogr B 799 2004 293 301
-
(2004)
J Chromatogr B
, vol.799
, pp. 293-301
-
-
Tubio, G.1
Nerli, B.2
Pico, G.3
-
30
-
-
0037193196
-
The surface exposed amino acid residues of monomeric proteins determine the partitioning in aqueous two-phase systems
-
K. Berggren, A. Wolf, J.A. Asenjo, B.A. Andrews, and F. Tjerneld The surface exposed amino acid residues of monomeric proteins determine the partitioning in aqueous two-phase systems Biochim Biophys Acta 1596 2002 253 268 The authors correlate the partitioning behavior of a set of proteins with several different amino acid-based hydrophobicity scales. Here the twist is that the authors weight the scales with the fraction of the protein surface occupied by each amino acid type for each protein. The predictive ability for partition coefficients is good. And, this approach should have implications for predicting solubility in salting-out processes and retention in hydrophobic chromatography.
-
(2002)
Biochim Biophys Acta
, vol.1596
, pp. 253-268
-
-
Berggren, K.1
Wolf, A.2
Asenjo, J.A.3
Andrews, B.A.4
Tjerneld, F.5
-
31
-
-
2642530983
-
N-Terminal tagged lactate dehydrogenase proteins: Evaluation of relative hydrophobicity by hydrophobic interaction chromatography and aqueous two-phase system partition
-
S. Fexby, H. Ihre, J. Van Alstine, and L. Bulow N-Terminal tagged lactate dehydrogenase proteins: evaluation of relative hydrophobicity by hydrophobic interaction chromatography and aqueous two-phase system partition J Chromatogr B 807 2004 25 31 The authors find a good correlation between protein partitioning behavior and retention on hydrophobic interaction chromatography (HIC) media. This is reminiscent of earlier work by Horvath correlating protein solubility in salting-out systems with retention in reversed-phase chromatography. Perhaps HIC can serve as a rapid screening platform to map out partitioning behavior.
-
(2004)
J Chromatogr B
, vol.807
, pp. 25-31
-
-
Fexby, S.1
Ihre, H.2
Van Alstine, J.3
Bulow, L.4
-
32
-
-
2642530194
-
Effect of electrostatic energy on partitioning of proteins in aqueous two-phase systems
-
A. Olivera-Nappa, G. Lagomarsino, B.A. Andrews, and J.A. Asenjo Effect of electrostatic energy on partitioning of proteins in aqueous two-phase systems J Chromatogr B 807 2004 81 86
-
(2004)
J Chromatogr B
, vol.807
, pp. 81-86
-
-
Olivera-Nappa, A.1
Lagomarsino, G.2
Andrews, B.A.3
Asenjo, J.A.4
-
33
-
-
0035809228
-
Characterization of aqueous two-phase partition systems by distribution analysis of radiolabeled analytes (DARA): Application to process definition and control in biorecovery
-
B. Lebreton, S.G. Walker, and A. Lyddiatt Characterization of aqueous two-phase partition systems by distribution analysis of radiolabeled analytes (DARA): application to process definition and control in biorecovery Biotechnol Bioeng 77 2002 329 339 The authors get their hands dirty here, quantitating the effects of varying amounts of contaminants on the phase composition in ATPE with a clever radiolabeled amino acid partitioning assay that reports on variations in tie line length. This type of information could be used to anticipate adjustments that might be needed in overall PEG/salt system composition to account for batch-to-batch variations in feedstocks.
-
(2002)
Biotechnol Bioeng
, vol.77
, pp. 329-339
-
-
Lebreton, B.1
Walker, S.G.2
Lyddiatt, A.3
-
34
-
-
0348143604
-
Two-phase affinity partitioning of animal cells: Implications of multipoint interactions
-
M.N. Gupta Birkhaeuser Verlag Basel
-
A. Kumar, M. Kamihira, and B. Mattiasson Two-phase affinity partitioning of animal cells: implications of multipoint interactions M.N. Gupta Methods for Affinity-Based Separations of Enzymes and Proteins 2002 Birkhaeuser Verlag Basel 163 180
-
(2002)
Methods for Affinity-Based Separations of Enzymes and Proteins
, pp. 163-180
-
-
Kumar, A.1
Kamihira, M.2
Mattiasson, B.3
-
35
-
-
0037140410
-
Understanding viral partitioning in two-phase aqueous nonionic micellar systems: 2. Effect of entrained micelle-poor domains
-
D.T. Kamei, J.A. King, D.I.C. Wang, and D. Blankschtein Understanding viral partitioning in two-phase aqueous nonionic micellar systems: 2. Effect of entrained micelle-poor domains Biotechnol Bioeng 78 2002 203 216
-
(2002)
Biotechnol Bioeng
, vol.78
, pp. 203-216
-
-
Kamei, D.T.1
King, J.A.2
Wang, D.I.C.3
Blankschtein, D.4
-
36
-
-
0348110469
-
Extraction of plasmid DNA from Escherichia coli cell lysate in a thermoseparating aqueous two-phase system
-
C. Kepka, J. Rhodin, R. Lemmens, F. Tjerneld, and P.-E. Gustavsson Extraction of plasmid DNA from Escherichia coli cell lysate in a thermoseparating aqueous two-phase system J Chromatogr A 1024 2004 95 104
-
(2004)
J Chromatogr a
, vol.1024
, pp. 95-104
-
-
Kepka, C.1
Rhodin, J.2
Lemmens, R.3
Tjerneld, F.4
Gustavsson, P.-E.5
-
37
-
-
4744352796
-
Permeation associated with three-phase-partitioning method on release of green fluorescent protein
-
T.C.V. Penna, E. Chiarini, and A.P. Jsdgunior Permeation associated with three-phase-partitioning method on release of green fluorescent protein Appl Biochem Biotechnol 105-108 2003 481 491
-
(2003)
Appl Biochem Biotechnol
, vol.105-108
, pp. 481-491
-
-
Penna, T.C.V.1
Chiarini, E.2
Jsdgunior, A.P.3
-
39
-
-
1842607757
-
Purification of recombinant green fluorescent protein by three-phase partitioning
-
S. Jain, R. Singh, and M.N. Gupta Purification of recombinant green fluorescent protein by three-phase partitioning J Chromatogr A 1035 2004 83 86
-
(2004)
J Chromatogr a
, vol.1035
, pp. 83-86
-
-
Jain, S.1
Singh, R.2
Gupta, M.N.3
-
40
-
-
1842561637
-
Three-phase partitioning for simultaneous renaturation and partial purification of Aspergillus niger xylanase
-
I. Roy, A. Sharma, and M.N. Gupta Three-phase partitioning for simultaneous renaturation and partial purification of Aspergillus niger xylanase Biochim Biophys Acta 1698 2004 107 110
-
(2004)
Biochim Biophys Acta
, vol.1698
, pp. 107-110
-
-
Roy, I.1
Sharma, A.2
Gupta, M.N.3
-
41
-
-
12144289986
-
Three-phase partitioning as an efficient method for extraction/ concentration of immunoreactive excreted-secreted proteins of Corynebacterium pseudotuberculosis
-
B.J.A. Paule, R. Meyer, L.F. Moura-Costa, R.C. Bahia, R. Carminati, L.F. Regis, V.L.C. Vale, S.M. Freire, I. Nascimento, and R. Schaer Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted-secreted proteins of Corynebacterium pseudotuberculosis Prot Exp Purif 34 2004 311 316
-
(2004)
Prot Exp Purif
, vol.34
, pp. 311-316
-
-
Paule, B.J.A.1
Meyer, R.2
Moura-Costa, L.F.3
Bahia, R.C.4
Carminati, R.5
Regis, L.F.6
Vale, V.L.C.7
Freire, S.M.8
Nascimento, I.9
Schaer, R.10
-
42
-
-
0036604782
-
Three phase partitioning of carbohydrate polymers: Separation and purification of alginates
-
A. Sharma, and M.N. Gupta Three phase partitioning of carbohydrate polymers: separation and purification of alginates Carb Polymers 48 2002 391 395
-
(2002)
Carb Polymers
, vol.48
, pp. 391-395
-
-
Sharma, A.1
Gupta, M.N.2
-
43
-
-
0037910083
-
A method for large scale purification of turnip peroxidase and its characterization
-
N. Singh, and J. Singh A method for large scale purification of turnip peroxidase and its characterization Prep Biochem Biotechnol 33 2003 125 135
-
(2003)
Prep Biochem Biotechnol
, vol.33
, pp. 125-135
-
-
Singh, N.1
Singh, J.2
-
44
-
-
0036145798
-
Three-phase affinity partitioning of proteins
-
I. Roy, and M.N. Gupta Three-phase affinity partitioning of proteins Anal Biochem 300 2002 11 14 Metal ions, Zn2+ and Cu2+ in this case, may be used to enhance the precipitation of metal ion binding proteins at the water-t-butanol interface in three-phase partitioning. Yields and purity of the target species, soybean trypsin inhibitor, exceeded that possible with metal affinity precipitation. The three-phase process also required lower metal ion concentrations than did straight metal affinity precipitation.
-
(2002)
Anal Biochem
, vol.300
, pp. 11-14
-
-
Roy, I.1
Gupta, M.N.2
-
45
-
-
0037568349
-
Separation of enzymes by sequential macroaffinity ligand-facilitated three-phase partitioning
-
A. Sharma, K. Mondal, and M.N. Gupta Separation of enzymes by sequential macroaffinity ligand-facilitated three-phase partitioning J Chromatogr A 995 2003 127 134
-
(2003)
J Chromatogr a
, vol.995
, pp. 127-134
-
-
Sharma, A.1
Mondal, K.2
Gupta, M.N.3
-
46
-
-
0037358560
-
Macroaffinity ligand-facilitated three-phase partitioning for purification of glucoamylase and pullulanase using alginate
-
K. Mondal, A. Sharma, and M.N. Gupta Macroaffinity ligand-facilitated three-phase partitioning for purification of glucoamylase and pullulanase using alginate Prot Exp Purif 28 2003 190 195
-
(2003)
Prot Exp Purif
, vol.28
, pp. 190-195
-
-
Mondal, K.1
Sharma, A.2
Gupta, M.N.3
-
47
-
-
0037358957
-
Macroaffinity ligand-facilitated three-phase partitioning (MLFTPP) of α-amylases using a modified alginate
-
K. Mondal, A. Sharma, R. Lata, and M.N. Gupta Macroaffinity ligand-facilitated three-phase partitioning (MLFTPP) of α-amylases using a modified alginate Biotechnol Prog 19 2003 493 494
-
(2003)
Biotechnol Prog
, vol.19
, pp. 493-494
-
-
Mondal, K.1
Sharma, A.2
Lata, R.3
Gupta, M.N.4
-
48
-
-
0037145328
-
Macroaffinity ligand-facilitated three-phase partitioning (MLFTPP) for purification of xylanase
-
A. Sharma, and M.N. Gupta Macroaffinity ligand-facilitated three-phase partitioning (MLFTPP) for purification of xylanase Biotechnol Bioeng 80 2002 228 232 A study notable for its introduction of the macroaffinity ligand facilitate three-phase partitioning concept. Here, a polymer (Eudragit S-100) to which a target enzyme had an affinity and that could be partitioned selectively to the interphase in a three-phase partitioning system, was used to enhance process selectivity. The work included promising purification performance studies carried out in the presence of yeast disruptates.
-
(2002)
Biotechnol Bioeng
, vol.80
, pp. 228-232
-
-
Sharma, A.1
Gupta, M.N.2
-
49
-
-
78649673749
-
Precipitation of proteins: Nonspecific and specific
-
A. Kumar, I.Y. Galaev, and Mattiasson B Precipitation of proteins: nonspecific and specific Biotechnol Bioproc 27 2003 225 275
-
(2003)
Biotechnol Bioproc
, vol.27
, pp. 225-275
-
-
Kumar, A.1
Galaev, I.Y.2
Mattiasson, B.3
-
50
-
-
0038739516
-
Protein purification by affinity precipitation
-
F. Hilbrig, and R. Freitag Protein purification by affinity precipitation J Chromatogr B 790 2003 79 90
-
(2003)
J Chromatogr B
, vol.790
, pp. 79-90
-
-
Hilbrig, F.1
Freitag, R.2
-
51
-
-
0037488298
-
Molecular thermodynamics for some applications in biotechnology
-
J.M. Prausnitz Molecular thermodynamics for some applications in biotechnology Pure Appl Chem 75 2003 859 873 If you were to read one paper on modern approaches to modeling protein solubility behavior, this would be a great choice. Prausnitz carefully and concisely explains how to construct an equation of state for a protein in solution based on a molecular interaction potential and how to apply thermodynamic equilibrium criteria to the equations of state to predict phase behavior. Very accessible to non-thermodynamicists.
-
(2003)
Pure Appl Chem
, vol.75
, pp. 859-873
-
-
Prausnitz, J.M.1
-
52
-
-
0036627825
-
Molecular thermodynamics for fluid-phase equilibria in aqueous two-protein systems
-
N. Von Solms, C.O. Anderson, H.W. Blanch, and J.M. Prausnitz Molecular thermodynamics for fluid-phase equilibria in aqueous two-protein systems AIChE J 48 2002 1292 1300 A first look at a multiprotein solution solubility model. The solubility of lysozyme and ovalbumin in ammonium sulfate solutions is addressed. Further progress will depend on developing predictive ability for model parameters as a function of ionic strength, pH and precipitant type.
-
(2002)
AIChE J
, vol.48
, pp. 1292-1300
-
-
Von Solms, N.1
Anderson, C.O.2
Blanch, H.W.3
Prausnitz, J.M.4
-
53
-
-
0037454676
-
Ultra scale-down approach for the prediction of full-scale recovery of ovine polycolonal immunoglobulins used in the manufacture of snake venom-specific Fab fragment
-
G. Neal, J. Christie, E. Keshavarz-Moore, and P.A. Shamlou Ultra scale-down approach for the prediction of full-scale recovery of ovine polycolonal immunoglobulins used in the manufacture of snake venom-specific Fab fragment Biotechnol Bioeng 81 2003 149 157 Rigorous modeling of the fluid dynamics of process-scale precipitation equipment was used to develop small-scale equipment that exposed protein and precipitates to similar time-integrated fluid stresses. One in a series of papers from University College London aimed at developing small-scale mimics of process-scale equipment for process development work.
-
(2003)
Biotechnol Bioeng
, vol.81
, pp. 149-157
-
-
Neal, G.1
Christie, J.2
Keshavarz-Moore, E.3
Shamlou, P.A.4
-
54
-
-
0036165573
-
Investigation of how agitation during precipitation, and subsequent processing affects the particle size distribution and separation of β-lactalbumin enriched whey protein precipitates
-
E.P. Byrne, and J.J. Fitzpatrick Investigation of how agitation during precipitation, and subsequent processing affects the particle size distribution and separation of β-lactalbumin enriched whey protein precipitates Biochem Eng J 10 2002 17 25
-
(2002)
Biochem Eng J
, vol.10
, pp. 17-25
-
-
Byrne, E.P.1
Fitzpatrick, J.J.2
-
55
-
-
4744369462
-
Manufacturing protein-based structures using a volatile acid
-
Boston, MA, United States, August 18-22
-
van der Wielen LAM, Hofland GW, Ottens M, Gulobovic M, Witkam G-J: Manufacturing protein-based structures using a volatile acid. In Abstracts of Papers, 224th ACS National Meeting, Boston, MA, United States, August 18-22, 2002. The use of pressurized carbon dioxide to adjust pH in an isoelectric precipitation process is described. Beyond the ease of precipitant removal by simply depressurizing the system, uniform, spherical precipitate particles were produced. Very interesting; the paper by Hofland and coworkers [56] provides more details.
-
(2002)
Abstracts of Papers, 224th ACS National Meeting
-
-
Van Der Wielen, L.A.M.1
Hofland, G.W.2
Ottens, M.3
Gulobovic, M.4
Witkam, G.-J.5
-
56
-
-
0034705825
-
Isoelectric precipitation of soybean protein using carbon dioxide as a volatile acid
-
G.W. Hofland, A. de Rijke, R. Thiering, L.A.M. van der Wielen, and G.-J. Witkamp Isoelectric precipitation of soybean protein using carbon dioxide as a volatile acid J Chromatogr B 743 2000 357 368
-
(2000)
J Chromatogr B
, vol.743
, pp. 357-368
-
-
Hofland, G.W.1
De Rijke, A.2
Thiering, R.3
Van Der Wielen, L.A.M.4
Witkamp, G.-J.5
-
57
-
-
1842614220
-
Precipitation and recovery of cytochrome c and hemoglobin using AOT and acetone
-
Y.-O. Shin, E. Rodil, and J.H. Vera Precipitation and recovery of cytochrome c and hemoglobin using AOT and acetone Sep Sci Tech 39 2004 1005 1019
-
(2004)
Sep Sci Tech
, vol.39
, pp. 1005-1019
-
-
Shin, Y.-O.1
Rodil, E.2
Vera, J.H.3
-
59
-
-
9144244168
-
Improved recovery of active recombinant laccase from maize seed
-
M.R. Bailey, S.L. Woodard, E. Callaway, K. Beifuss, M. Magallanes-Lundback, J.R. Lane, M.E. Horn, H. Mallubhotla, D.D. Delaney, and M. Ward Improved recovery of active recombinant laccase from maize seed Appl Microbiol Biotechnol 63 2004 390 397
-
(2004)
Appl Microbiol Biotechnol
, vol.63
, pp. 390-397
-
-
Bailey, M.R.1
Woodard, S.L.2
Callaway, E.3
Beifuss, K.4
Magallanes-Lundback, M.5
Lane, J.R.6
Horn, M.E.7
Mallubhotla, H.8
Delaney, D.D.9
Ward, M.10
-
60
-
-
0038072717
-
6-tagged lactate dehydrogenase using poly(vinylimidazole-co-N- isopropylacrylamide) copolymers
-
6-tagged lactate dehydrogenase using poly(vinylimidazole-co-N-isopropylacrylamide) copolymers Enz Micro Tech 33 2003 113 117 Mattiasson's group has pioneered the use of modified, thermally responsive polymers for metal affinity precipitation.
-
(2003)
Enz Micro Tech
, vol.33
, pp. 113-117
-
-
Kumar, A.1
Khalil, A.A.M.2
Galaev, I.Y.3
Mattiasson, B.4
-
61
-
-
0142104893
-
Purification of histidine-tagged single-chain Fv-antibody fragments by metal chelate affinity precipitation using thermoresponsive copolymers
-
A. Kumar, P.-O. Wahlund, C. Kepka, I.Y. Galaev, and B. Mattiasson Purification of histidine-tagged single-chain Fv-antibody fragments by metal chelate affinity precipitation using thermoresponsive copolymers Biotechnol Bioeng 84 2003 494 503 Another example of the use of modified, thermally responsive polymers for metal affinity precipitation; the purification and yield for the single-chain antibody reported were impressive.
-
(2003)
Biotechnol Bioeng
, vol.84
, pp. 494-503
-
-
Kumar, A.1
Wahlund, P.-O.2
Kepka, C.3
Galaev, I.Y.4
Mattiasson, B.5
-
62
-
-
0036953259
-
Affinity precipitation separation of DNA binding protein using block conjugate composed of poly(N-isopropylacrylamide) grafted double-stranded DNA and double-stranded DNA containing a target sequence
-
N. Soh, D. Umeno, Z. Tang, M. Murata, and M. Maeda Affinity precipitation separation of DNA binding protein using block conjugate composed of poly(N-isopropylacrylamide) grafted double-stranded DNA and double-stranded DNA containing a target sequence Anal Sci 18 2002 1295 1299
-
(2002)
Anal Sci
, vol.18
, pp. 1295-1299
-
-
Soh, N.1
Umeno, D.2
Tang, Z.3
Murata, M.4
Maeda, M.5
-
63
-
-
0032739304
-
Purification of recombinant proteins by fusion with thermally responsive polypeptides
-
D.E. Meyer, and A. Chilkoti Purification of recombinant proteins by fusion with thermally responsive polypeptides Nat Biotechnol 17 1999 1112 1115
-
(1999)
Nat Biotechnol
, vol.17
, pp. 1112-1115
-
-
Meyer, D.E.1
Chilkoti, A.2
-
64
-
-
0037420917
-
One-step metal-affinity purification of histidine-tagged proteins by temperature-triggered precipitation
-
H. Stiborova, J. Kostal, A. Mulchandani, and W. Chen One-step metal-affinity purification of histidine-tagged proteins by temperature- triggered precipitation Biotechnol Bioeng 82 2003 605 611 A report on the use of affinity-modified ELPs - recombinant proteins with a repeating VPGVG sequence - as thermally reversible precipitation agents. Specificity was conferred by incorporation of imidazole groups within the ELPs and targeting His-tagged proteins with metal ions. This is exceedingly clever technology.
-
(2003)
Biotechnol Bioeng
, vol.82
, pp. 605-611
-
-
Stiborova, H.1
Kostal, J.2
Mulchandani, A.3
Chen, W.4
-
65
-
-
0345832412
-
Affinity precipitation of Aspergillus niger pectinase by microwave-treated alginate
-
K. Mondal, P. Mehta, and M.N. Gupta Affinity precipitation of Aspergillus niger pectinase by microwave-treated alginate Prot Exp Purif 33 2004 104 109
-
(2004)
Prot Exp Purif
, vol.33
, pp. 104-109
-
-
Mondal, K.1
Mehta, P.2
Gupta, M.N.3
-
66
-
-
1542288211
-
Preparative separation of proteins using centrifugal precipitation chromatography based on solubility in ammonium sulfate solution
-
H. Yu, and Y. Ito Preparative separation of proteins using centrifugal precipitation chromatography based on solubility in ammonium sulfate solution Prep Biochem Biotechnol 34 2004 1 12 A progress report on the CPC technique in which a precipitant gradient is established in a long channel within a centrifuge by countercurrent flow of buffer and precipitant solutions on opposite sides of an ultrafiltration/dialysis membrane. The mixture to be separated is fed with the buffer while the slope of the precipitant gradient is gradually reduced. This results in repeated precipitation and dissolution, with substances eluting from the centrifuge in order of most soluble to least soluble. CPC sounds more complicated than it actually is and reported separations for several different crude mixtures are impressive.
-
(2004)
Prep Biochem Biotechnol
, vol.34
, pp. 1-12
-
-
Yu, H.1
Ito, Y.2
-
67
-
-
0036383317
-
Centrifugal precipitation chromatography: Novel fractionation method for biopolymers, based on their solubility
-
Y. Ito Centrifugal precipitation chromatography: novel fractionation method for biopolymers, based on their solubility J Liq Chromatogr Rel Tech 25 2002 2039 2064
-
(2002)
J Liq Chromatogr Rel Tech
, vol.25
, pp. 2039-2064
-
-
Ito, Y.1
-
68
-
-
0347719515
-
Protein crystallization: From HTS to kilogram-scale
-
V. Klyushnichenko Protein crystallization: from HTS to kilogram-scale Curr Opin Drug Discovery Dev 6 2003 848 854 Overview of the state-of-the-art of protein crystallization. Summary of impact of process variables and vessel design on crystal morphology and process yield at larger scale. High-throughput screening systems are being developed in 96-well-plate and smaller formats that, combined with robotic liquid handling and novel analysis methods, are enabling the screening of broader ranges of conditions. Some initial evidence of predictive behavior upon scaleup is emerging.
-
(2003)
Curr Opin Drug Discovery Dev
, vol.6
, pp. 848-854
-
-
Klyushnichenko, V.1
-
69
-
-
4744359925
-
Protein crystallization for large-scale bioseparation
-
E.K. Lee, and W.S. Kim Protein crystallization for large-scale bioseparation Biotechnol Bioproc 27 2003 277 320 A comprehensive and very theoretical review of crystallization theory, including equipment design, the impact of impurities, and considerations for process development of a crystallization process.
-
(2003)
Biotechnol Bioproc
, vol.27
, pp. 277-320
-
-
Lee, E.K.1
Kim, W.S.2
-
70
-
-
0037393408
-
A procedure for setting up high-throughput nanolitre crystallization experiments. II. Crystallization results
-
J. Brown, T.S. Walter, L. Carter, N.G.A. Abrescia, A.R. Aricescu, T.D. Batuwangala, L.E. Bird, N. Brown, P.P. Chamberlain, and S. Davis A procedure for setting up high-throughput nanolitre crystallization experiments. II. Crystallization results J Appl Crystallogr 36 2003 315 318
-
(2003)
J Appl Crystallogr
, vol.36
, pp. 315-318
-
-
Brown, J.1
Walter, T.S.2
Carter, L.3
Abrescia, N.G.A.4
Aricescu, A.R.5
Batuwangala, T.D.6
Bird, L.E.7
Brown, N.8
Chamberlain, P.P.9
Davis, S.10
-
71
-
-
4744364756
-
-
Microfluidic chip for biomolecule crystallization. US Patent Application: US 2004048388; 2004.
-
Teragenics (Gilbert J, Trikha J): Microfluidic chip for biomolecule crystallization. US Patent Application: US 2004048388; 2004.
-
-
-
Gilbert, J.1
Trikha, J.2
-
72
-
-
0035313212
-
Membrane separations in biotechnology
-
R. van Reis, and A.L. Zydney Membrane separations in biotechnology Curr Opin Biotechnol 12 2001 208 211 Previous review on membrane separation in this journal.
-
(2001)
Curr Opin Biotechnol
, vol.12
, pp. 208-211
-
-
Van Reis, R.1
Zydney, A.L.2
-
73
-
-
0037056573
-
High-performance tangential flow filtration: A highly selective membrane separation process
-
Charles C, Adams G, Kuriyel R, Bolton G, Seilly A: High-performance tangential flow filtration: a highly selective membrane separation process. Desalination 144:133-136.
-
Desalination
, vol.144
, pp. 133-136
-
-
Charles, C.1
Adams, G.2
Kuriyel, R.3
Bolton, G.4
Seilly, A.5
-
74
-
-
0000902780
-
Effect of membrane charge on high performance tangential flow filtration separation
-
G. Bolton, R. Kuriyel, and S. Orlando Effect of membrane charge on high performance tangential flow filtration separation Adv Filt Sep Tech 13a 1999 537 544
-
(1999)
Adv Filt Sep Tech
, vol.13
, pp. 537-544
-
-
Bolton, G.1
Kuriyel, R.2
Orlando, S.3
-
75
-
-
1842713256
-
Separation of protein charge variants by ultrafiltration
-
M.F. Ebersold, and A.L. Zydney Separation of protein charge variants by ultrafiltration Biotechnol Prog 20 2004 543 549 A compelling demonstration of separation of nearly identical proteins by ultrafiltration.
-
(2004)
Biotechnol Prog
, vol.20
, pp. 543-549
-
-
Ebersold, M.F.1
Zydney, A.L.2
-
76
-
-
1242270470
-
A two-stage ultrafiltration process for fractionation of whey protein isolate
-
Cheang B, Zydney AL: A two-stage ultrafiltration process for fractionation of whey protein isolate. J Mem Sci 231:159-167.
-
J Mem Sci
, vol.231
, pp. 159-167
-
-
Cheang, B.1
Zydney, A.L.2
-
77
-
-
0343527982
-
Purification of lysozyme using ultrafiltration
-
R. Ghosh, and Z.F. Cui Purification of lysozyme using ultrafiltration Biotechnol Bioeng 68 2000 191 203
-
(2000)
Biotechnol Bioeng
, vol.68
, pp. 191-203
-
-
Ghosh, R.1
Cui, Z.F.2
-
78
-
-
0034609044
-
Purification of plasmid DNA by tangential flow filtration
-
D.W. Kahn, M.D. Butlet, D.L. Cohen, M. Gordon, J.W. Kahn, and M.E. Winkler Purification of plasmid DNA by tangential flow filtration Biotechnol Bioeng 69 2000 101 106
-
(2000)
Biotechnol Bioeng
, vol.69
, pp. 101-106
-
-
Kahn, D.W.1
Butlet, M.D.2
Cohen, D.L.3
Gordon, M.4
Kahn, J.W.5
Winkler, M.E.6
-
79
-
-
0037402921
-
Removal of RNA impurities by tangential flow filtration in an RNase-free plasmid DNA purification process
-
A. Eon-Duval, R.H. MacDuff, C.A. Fisher, M.J. Harris, and C. Brook Removal of RNA impurities by tangential flow filtration in an RNase-free plasmid DNA purification process Anal Biochem 316 2003 66 73
-
(2003)
Anal Biochem
, vol.316
, pp. 66-73
-
-
Eon-Duval, A.1
MacDuff, R.H.2
Fisher, C.A.3
Harris, M.J.4
Brook, C.5
-
80
-
-
2542498803
-
Highly selective membrane in protein ultrafiltration
-
M. Feins, and K.K. Sirkar Highly selective membrane in protein ultrafiltration Biotechnol Bioeng 86 2004 603 611 Staged approach to enhancing selectivity in traditional ultrafiltration.
-
(2004)
Biotechnol Bioeng
, vol.86
, pp. 603-611
-
-
Feins, M.1
Sirkar, K.K.2
-
82
-
-
4744357526
-
[Monolithic] tubes
-
A. Podgornik, M. Barut, I. Mihelic, and A. Strancar [Monolithic] tubes J Chromatogr Lib 67 2003 77 101 Review that focuses on recent developments in polymerization strategies to produce larger monolithic tubes with similar separation properties to smaller tubes and disks.
-
(2003)
J Chromatogr Lib
, vol.67
, pp. 77-101
-
-
Podgornik, A.1
Barut, M.2
Mihelic, I.3
Strancar, A.4
-
84
-
-
4744367007
-
Monolithic continuous beds prepared from water-soluble acrylamide-based monomers
-
A. Maruska Monolithic continuous beds prepared from water-soluble acrylamide-based monomers J Chromatogr Lib 67 2003 143 172
-
(2003)
J Chromatogr Lib
, vol.67
, pp. 143-172
-
-
Maruska, A.1
-
85
-
-
3142643857
-
Transfer of gradient chromatographic methods for protein separation to convective interaction media monolithic columns
-
P. Milavec Zmak, H. Podgornik, J. Jancar, A. Podgornik, and A. Strancar Transfer of gradient chromatographic methods for protein separation to convective interaction media monolithic columns J Chromatogr A 1006 2003 195 205 Systematic, mathematical scale up approaches for monolithic axial flow columns and radial flow tubes. This was done using the world's most chromatographed proteins, but the authors also investigated purification of peroxidases from a 'real world' fungal broth. Scale up to an 800 mL monolithic radial flow tube was demonstrated.
-
(2003)
J Chromatogr a
, vol.1006
, pp. 195-205
-
-
Milavec Zmak, P.1
Podgornik, H.2
Jancar, J.3
Podgornik, A.4
Strancar, A.5
-
86
-
-
0042347638
-
Chromatographic performance of monolithic and particulate stationary phases. Hydrodynamics and adsorption capacity
-
F.C. Leinweber, and U. Tallarek Chromatographic performance of monolithic and particulate stationary phases. Hydrodynamics and adsorption capacity J Chromatogr A 1006 2003 207 228 Mathematical approach to treating flow through continuous monoliths and determining the equivalent particle (sphere) dimensions that characterize the corresponding hydrodynamic and adsorption behavior in a particulate bed. The paper includes scanning electron microscopy pictures of various commercially available monoliths, including both organic polymer monoliths and silica based monoliths.
-
(2003)
J Chromatogr a
, vol.1006
, pp. 207-228
-
-
Leinweber, F.C.1
Tallarek, U.2
-
87
-
-
0038401243
-
Application of semi-industrial monolithic columns for downstream processing of clotting factor IX
-
K. Branovic, A. Buchacher, M. Barut, A. Strancar, and D. Josic Application of semi-industrial monolithic columns for downstream processing of clotting factor IX J Chromatogr B 790 2003 175 182 Demonstration of superior performance of monoliths in a commercially relevant separation (both faster and higher purification factor) and one of the first papers dealing with larger scale tubes (up to 500mL).
-
(2003)
J Chromatogr B
, vol.790
, pp. 175-182
-
-
Branovic, K.1
Buchacher, A.2
Barut, M.3
Strancar, A.4
Josic, D.5
-
88
-
-
0037023369
-
Protein separation using membrane chromatography: Opportunities and challenges
-
R. Ghosh Protein separation using membrane chromatography: Opportunities and challenges J Chromatogr A 952 2002 13 27 A recent detailed catalog of membrane chromatography studies.
-
(2002)
J Chromatogr a
, vol.952
, pp. 13-27
-
-
Ghosh, R.1
-
89
-
-
0037224686
-
Rethinking the economics of chromatography. New technologies and hidden costs
-
T.N. Warner, and S. Nochureson Rethinking the economics of chromatography. New technologies and hidden costs Biopharm Intl 16 2003 58 60 Economic comparison of membrane chromatography and packed-bed chromatography.
-
(2003)
Biopharm Intl
, vol.16
, pp. 58-60
-
-
Warner, T.N.1
Nochureson, S.2
-
90
-
-
20744449554
-
Membrane adsorbers. A cutting edge process technology at the threshold
-
U. Gottschalk, Fischer-Fruehholz, and O. Reif Membrane adsorbers. A cutting edge process technology at the threshold Bioprocess Intl 2004 56 65
-
(2004)
Bioprocess Intl
, pp. 56-65
-
-
Ast, F.1
Fischer-Fruehholz2
Reif, O.3
-
91
-
-
0037424580
-
Adsorptive membrane chromatography for purification of plasmid DNA
-
M.A. Teeters, S.E. Conrardy, B.L. Thomas, T.W. Root, and E.N. Lightfoot Adsorptive membrane chromatography for purification of plasmid DNA J Chromatogr A 980 2003 165 173
-
(2003)
J Chromatogr a
, vol.980
, pp. 165-173
-
-
Teeters, M.A.1
Conrardy, S.E.2
Thomas, B.L.3
Root, T.W.4
Lightfoot, E.N.5
-
92
-
-
0037938790
-
Evaluation of an ion-exchange membrane for the purification of plasmid DNA
-
H.N. Endres, J.A.C. Johnson, C.A. Ross, J.K. Welp, and M.R. Etzel Evaluation of an ion-exchange membrane for the purification of plasmid DNA Biotechnol Appl Biochem 37 2003 259 266
-
(2003)
Biotechnol Appl Biochem
, vol.37
, pp. 259-266
-
-
Endres, H.N.1
Johnson, J.A.C.2
Ross, C.A.3
Welp, J.K.4
Etzel, M.R.5
-
93
-
-
6344285524
-
Case study: Capacity challenges in chromatography-based purification of plasmid DNA
-
A.S. Rathore A. Velayudhan Marcel Dekker Inc New York
-
S.L. Sagar, M.P. Watson, A.L. Lee, and Y.G. Chau Case study: capacity challenges in chromatography-based purification of plasmid DNA A.S. Rathore A. Velayudhan Scale-up and Optimization in Preparative Chromatography 2002 Marcel Dekker Inc New York 251 272 Study demonstrating the low capacity for plasmid DNA in chromatography and its enhancement by solution additives.
-
(2002)
Scale-up and Optimization in Preparative Chromatography
, pp. 251-272
-
-
Sagar, S.L.1
Watson, M.P.2
Lee, A.L.3
Chau, Y.G.4
-
94
-
-
0035847162
-
Membrane ion-exchange chromatography for process-scale antibody purification
-
H.L. Knudsen, R.L. Fahmer, Y. Xu, L.A. Norling, and G.S. Blank Membrane ion-exchange chromatography for process-scale antibody purification J Chromatogr A 907 2001 145 154 A thorough evaluation of industrial application of membrane chromatography.
-
(2001)
J Chromatogr a
, vol.907
, pp. 145-154
-
-
Knudsen, H.L.1
Fahmer, R.L.2
Xu, Y.3
Norling, L.A.4
Blank, G.S.5
-
95
-
-
0035115607
-
Downstream processing of bovine lactoferrin from sweet whey
-
R. Ulber, K. Plate, T. Weiss, W. Demmer, H. Buchholz, and T. Scheper Downstream processing of bovine lactoferrin from sweet whey Acta Biotechnol 21 2001 27 34
-
(2001)
Acta Biotechnol
, vol.21
, pp. 27-34
-
-
Ulber, R.1
Plate, K.2
Weiss, T.3
Demmer, W.4
Buchholz, H.5
Scheper, T.6
-
96
-
-
0034759325
-
Manufacture and quality control of CAMPATH-1 antibodies for clinical trials
-
J. Phillips, A. Drumm, P. Harrison, P. Bird, K. Bhamra, E. Berrie, and G. Hale Manufacture and quality control of CAMPATH-1 antibodies for clinical trials Cytotherapy 3 2001 233 242
-
(2001)
Cytotherapy
, vol.3
, pp. 233-242
-
-
Phillips, J.1
Drumm, A.2
Harrison, P.3
Bird, P.4
Bhamra, K.5
Berrie, E.6
Hale, G.7
-
97
-
-
0036021998
-
An integrated process for mammalian cell perfusion cultivation and product purification using a dynamic filter
-
L.R. Castilho, F.B. Anspach, and W.-D. Deckwer An integrated process for mammalian cell perfusion cultivation and product purification using a dynamic filter Biotechnol Prog 18 2002 776 781 The authors describe the use of a rotating disk filter functionalized with protein A to capture a monoclonal antibody from whole Chinese hamster ovary cell broths in a cell recycle mode, yielding true upstream/downstream integration.
-
(2002)
Biotechnol Prog
, vol.18
, pp. 776-781
-
-
Castilho, L.R.1
Anspach, F.B.2
Deckwer, W.-D.3
-
98
-
-
0037105919
-
Comparison of affinity membranes for the purification of immunoglobins
-
L.R. Castilho, F.B. Anspach, and W.-D. Deckwer Comparison of affinity membranes for the purification of immunoglobins J Mem Sci 207 2002 253 264
-
(2002)
J Mem Sci
, vol.207
, pp. 253-264
-
-
Castilho, L.R.1
Anspach, F.B.2
Deckwer, W.-D.3
-
99
-
-
0036809548
-
Separation of IgG from human plasma using thiophilic hollow fiber membranes
-
Y. Coffinier, C. Legallais, and M.A. Vijayalakshmi Separation of IgG from human plasma using thiophilic hollow fiber membranes J Mem Sci 28 2002 13 22
-
(2002)
J Mem Sci
, vol.28
, pp. 13-22
-
-
Coffinier, Y.1
Legallais, C.2
Vijayalakshmi, M.A.3
-
100
-
-
0036007994
-
Separation of MBP fusion proteins through affinity membranes
-
F. Cattoli, and G.C. Sarti Separation of MBP fusion proteins through affinity membranes Biotechnol Prog 18 2002 94 100
-
(2002)
Biotechnol Prog
, vol.18
, pp. 94-100
-
-
Cattoli, F.1
Sarti, G.C.2
-
101
-
-
0037454634
-
Immunoaffinity removal of xenoreactive antibodies using modified dialysis or microfiltration membranes
-
S. Karoor, J. Molina, C.R. Buchmann, C. Colton, J.S. Logan, and L.W. Henderson Immunoaffinity removal of xenoreactive antibodies using modified dialysis or microfiltration membranes Biotech Bioeng 81 2003 134 148
-
(2003)
Biotech Bioeng
, vol.81
, pp. 134-148
-
-
Karoor, S.1
Molina, J.2
Buchmann, C.R.3
Colton, C.4
Logan, J.S.5
Henderson, L.W.6
-
102
-
-
0035188126
-
Molecular imprinting: At the edge of the third millennium
-
S.A. Piletsky, S. Alcock, and A.P.F. Turner Molecular imprinting: at the edge of the third millennium Trends Biotechnol 19 2001 9 12 Review of molecular imprinting.
-
(2001)
Trends Biotechnol
, vol.19
, pp. 9-12
-
-
Piletsky, S.A.1
Alcock, S.2
Turner, A.P.F.3
-
103
-
-
0033532285
-
Receptor and transport properties of imprinted polymer membranes - A review
-
S.A. Piletsky, T.L. Panasyuk, E.V. Piletskaya, I.A. Nicholls, and M. Ulbricht Receptor and transport properties of imprinted polymer membranes - a review J Mem Sci 157 1999 263 278
-
(1999)
J Mem Sci
, vol.157
, pp. 263-278
-
-
Piletsky, S.A.1
Panasyuk, T.L.2
Piletskaya, E.V.3
Nicholls, I.A.4
Ulbricht, M.5
-
104
-
-
0037426677
-
Integration of production and aqueous two-phase systems extraction of extracellular Fusarium solani pisi cutinase fusion proteins
-
M.T. Cunha, M.J.L. Costa, C.R.C. Calado, L.P. Fonseca, M.R. Aires-Barros, and J.M.S. Cabral Integration of production and aqueous two-phase systems extraction of extracellular Fusarium solani pisi cutinase fusion proteins J Biotechnol 100 2003 55 64
-
(2003)
J Biotechnol
, vol.100
, pp. 55-64
-
-
Cunha, M.T.1
Costa, M.J.L.2
Calado, C.R.C.3
Fonseca, L.P.4
Aires-Barros, M.R.5
Cabral, J.M.S.6
-
105
-
-
0035553492
-
Parameters influencing protein extraction for whole broths in detergent based aqueous two-phase systems
-
K. Selber, A. Collen, T. Hyytia, M. Penttila, F. Tjerneld, and M.-R. Kula Parameters influencing protein extraction for whole broths in detergent based aqueous two-phase systems Bioseparation 10 2002 229 236
-
(2002)
Bioseparation
, vol.10
, pp. 229-236
-
-
Selber, K.1
Collen, A.2
Hyytia, T.3
Penttila, M.4
Tjerneld, F.5
Kula, M.-R.6
-
106
-
-
0036729223
-
Aqueous two-phase systems for the recovery of a recombinant viral coat protein from Escherichia coli
-
M. Rito-Palomares, and A.P.J. Middelberg Aqueous two-phase systems for the recovery of a recombinant viral coat protein from Escherichia coli J Chem Tech Biotechnol 77 2002 1025 1029
-
(2002)
J Chem Tech Biotechnol
, vol.77
, pp. 1025-1029
-
-
Rito-Palomares, M.1
Middelberg, A.P.J.2
-
107
-
-
2642519497
-
Recovery in aqueous two-phase systems of lutein produced by the green microalga Chlorella protothecoides
-
M. Cisneros, J. Benavides, C.H. Brenes, and M. Rito-Palomares Recovery in aqueous two-phase systems of lutein produced by the green microalga Chlorella protothecoides J Chromatogr B 807 2004 105 110
-
(2004)
J Chromatogr B
, vol.807
, pp. 105-110
-
-
Cisneros, M.1
Benavides, J.2
Brenes, C.H.3
Rito-Palomares, M.4
-
108
-
-
0037189835
-
Process integration using aqueous two-phase partition for the recovery of intracellular proteins
-
M. Rito-Palomares, and A. Lyddiatt Process integration using aqueous two-phase partition for the recovery of intracellular proteins Chem Eng J 87 2002 313 319 A description of the direct integration of cell disruption and aqueous two-phase extraction via use of a bead mill to both disrupt yeast cells and homogenize the system to extract an intracellular enzyme. The performance of this system in terms of PEG-phase yield of enzyme was indistinguishable from that of separate disruption and extraction steps. However, a complicating factor in this approach is that the partitioning behavior of the cell debris in some cases was opposite to that of the separate unit operations. A logical integration somewhat complicated by the vagaries of cell debris partitioning.
-
(2002)
Chem Eng J
, vol.87
, pp. 313-319
-
-
Rito-Palomares, M.1
Lyddiatt, A.2
-
109
-
-
0038640274
-
Collection and purification of parasporal crystals from Bacillus thuringiensis by aqueous two-phase extraction
-
D.-Q. Lin, S.-J. Yao, L.-H. Mei, and Z.-Q. Zhu Collection and purification of parasporal crystals from Bacillus thuringiensis by aqueous two-phase extraction Sep Sci Tech 38 2003 1665 1680
-
(2003)
Sep Sci Tech
, vol.38
, pp. 1665-1680
-
-
Lin, D.-Q.1
Yao, S.-J.2
Mei, L.-H.3
Zhu, Z.-Q.4
-
110
-
-
0037160333
-
Process integration of separation of amino acids by a temperature-induced aqueous two-phase system
-
M. Li, Z.-Q. Zhu, and A.E. Rodrigues Process integration of separation of amino acids by a temperature-induced aqueous two-phase system Ind Eng Chem Res 41 2002 251 256
-
(2002)
Ind Eng Chem Res
, vol.41
, pp. 251-256
-
-
Li, M.1
Zhu, Z.-Q.2
Rodrigues, A.E.3
-
111
-
-
0037198877
-
Controlled shear affinity filtration (CSAF): A new technology for integration of cell separation and protein isolation from mammalian cell cultures
-
J.H. Vogel, B. Anspach, K.-H. Kroner, J.M. Piret, and C.A. Haynes Controlled shear affinity filtration (CSAF): a new technology for integration of cell separation and protein isolation from mammalian cell cultures Biotechnol Bioeng 78 2002 806 814 A thorough and compelling study of the use of a rotating disk filter functionalized with lysine to capture tPA, a secreted product, from whole Chinese hamster ovary cell broths. Vogel and co-workers paid particular attention to the fluid mechanics of the filter device, using a cone-and-plate geometry to generate a uniform filter surface shear rate - motivating the 'CSAF' moniker - that produces sharp breakthrough curves due to more homogeneous transmembrane flow; the sharper the breakthrough curve, the greater the dynamic binding capacity. The cone-and-plate design is particularly clever in its simplicity and effectiveness.
-
(2002)
Biotechnol Bioeng
, vol.78
, pp. 806-814
-
-
Vogel, J.H.1
Anspach, B.2
Kroner, K.-H.3
Piret, J.M.4
Haynes, C.A.5
-
112
-
-
0037137887
-
Host selection as a downstream strategy: Polyelectrolyte precipitation of β-glucuronidase from plant extracts
-
10 tail actually reduced PEI precipitation performance in the oilseed extracts, as it was more difficult to re-dissolve the resulting precipitate.
-
(2002)
Biotechnol Bioeng
, vol.77
, pp. 148-154
-
-
Menkhaus, T.J.1
Eriksson, S.U.2
Whitson, P.B.3
Glatz, C.E.4
-
113
-
-
0037171135
-
The use of high pressure for separation and production of bioactive molecules
-
P. Lemay The use of high pressure for separation and production of bioactive molecules Biochim Biophys Acta 1595 2002 357 366
-
(2002)
Biochim Biophys Acta
, vol.1595
, pp. 357-366
-
-
Lemay, P.1
-
114
-
-
0038377222
-
Protein loading, elution, and resolution behavior in a novel device that integrates ultrafiltration and chromatographic separation
-
X.-P. Dai, S. Majumdar, R.G. Luo, and K.K. Sirkar Protein loading, elution, and resolution behavior in a novel device that integrates ultrafiltration and chromatographic separation Biotechnol Bioeng 83 2003 125 139
-
(2003)
Biotechnol Bioeng
, vol.83
, pp. 125-139
-
-
Dai, X.-P.1
Majumdar, S.2
Luo, R.G.3
Sirkar, K.K.4
|