Expanded bed adsorption for purification of alcohol dehydrogenase using a dye-iminodiacetic acid matrix

Journal of Bioscience and Bioengineering

Volumn 97, Issue 4, 2004, Pages 284-287

  Hidayat, Chusnul ^a   Takagi, Mutsumi ^a   Yoshida, Toshiomi ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Alcohol dehydrogenase; Dye; Expanded bed; Metal affinity; Unclarified yeast homogenate

Indexed keywords

ADSORPTION; ALCOHOLS; CELLS; CHROMATOGRAPHY; IONS; YEAST;

CELL DEBRIS; EXPANDED BED ADSORPTION (EBA);

BIOMEDICAL ENGINEERING;

ALCOHOL DEHYDROGENASE; IMIDAZOLE;

ARTICLE; CONTROLLED STUDY; ELUTION; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; EXPANDED BED ADSORPTION; ION EXCHANGE; NONHUMAN; PROTEIN ANALYSIS; SACCHAROMYCES CEREVISIAE;

NEGIBACTERIA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 2342510245     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.97.284     Document Type: Article

References (20)

1. Galliot, F. P., Gleason, C., Wilson, J. J., and Zwarick, J.: Fluidized bed adsorption for whole broth extraction. Biotechnol. Prog., 6, 370-375 (1990).
2. Chang, Y. K., McCreath, G. E., and Chase, H. A.: Development of an expanded bed technique for an affinity purification of G6PDH from unclarified yeast cell homogenate. Biotechnol. Bioeng., 48, 355-366 (1995).
3. Frej, A-K. B., Hjorth, R., and Hammarström, Å.: Pilot scale recovery of recombinant annexin V from unclarified Escherichia coli homogenate using expanded bed adsorption. Biotechnol. Bioeng., 44, 922-929 (1994).
4. Chang, Y. K. and Chase, H. A.: Ion exchange purification of G6PDH from unclarified yeast cell homogenates using expanded bed adsorption. Biotechnol. Bioeng., 49, 204-216 (1996).
5. Thömmes, J., Halfar, M., Lenz, S., and Kula, M-R.: Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption. Biotechnol. Bioeng., 45, 205-211 (1995).
6. Frej, A-K. B.: Expanded bed adsorption for recovery of re-naturated human recombinant interleukin 8 from Escherichia coli inclusion bodies. Bioseparation, 6, 265-271 (1996).
7. Chase, H. A. and Draeger, N. M.: Affinity purification of proteins using expanded beds. J. Chromatogr. A, 597, 129-145 (1992).
8. Fernández-Lahore, H. M., Geilenkirchen, S., Boldt, K., Nagel, A., Kula, M-R., and Thömmes, J.: The influence of cell adsorbent interactions on protein adsorption in expanded beds. J. Chromatogr., 873, 195-208 (2000).
9. Michel, P., Torkkeli, T., Karp, M., and Oker-Blom, C.: Expression and purification of pohyhistidine-tagged firely luciferase in insect cell - a potential alternative for process scale-up. J. Biotechnol., 85, 49-56 (2001).
10. Clemmltt, R. H. and Chase, H. A.: Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal-affinity expanded-bed adsorption. Biotechnol. Bioeng., 67, 206-216 (2000).
11. Gibert, S., Bakalara, N., and Santarelli, X.: Three-step chromatographic purification procedure for the purification of a His-tag recombinant kinesin over expressed in E. coli. J. Chromatogr. B, 737, 143-150 (2000).
12. Clemmitt, R. H. and Chase, H. A.: Immobilized metal-affinity chromatography of ß-galactosidase from unclarified Escherichia coli homogenates using expanded bed adsorption. J. Chromatogr. A, 874, 27-43 (2000).
13. Hidayat, C., Nakajima, M., Takagi, M., and Yoshida, T.: Development of new dye-metal agarose-coated alumina matrix and elution strategy for purification of alcohol dehydrogenase. J. Biosci. Bioeng., 95, 133-138 (2003).
14. Willoughby, N. A., Kirschner, T., Smith, M. P., Hjorth, R., and Tichener-Hooker, N. J.: Immobilized metal ion affinity chromatography purification of alcohol dehydrogenase from baker's yeast using an expanded bed adsorption system. J. Chromatogr. A, 840, 195-204 (1999).
15. Jornvall, H., Eklund, H., and Branden, C. I.: Subunit conformational of yeast alcohol dehydrogenase. J. Biol. Chem., 253, 8414-8419 (1978).
16. Sulkowski, E.: Purification of proteins by IMAC. Trends Biotechnol., 3, 1-7 (1985).
17. Hemdan, E. S., Zhao, Y. J., Sulkowski, E., and Porath, J.: Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography. Proc. Natl. Acad. Sci. USA, 86, 1811-1815 (1989).
18. Gaberc-Porekar, V. and Menart, V.: Perspectives of immobilized-metal affinity chromatography. J. Biochem. Biophys. Methods, 49, 335-360 (2001).
19. Smith, M. P., Bulmer, M. A., Hjorth, R., and Titchener-Hooker, N. J.: Hydrophobic interaction ligand selection and scale-up of an expanded bed separation of an intracellular enzyme from Saccharomyces cerevisiae. J. Chromatogr. A, 968, 121-128 (2002).
20. Denizli, A., Yavuz, H., and Arica, Y.: Monosize and nonporous p(HEMA-co-MMA) microparticles designed as dye-and metal-chelate affinity sorbents. Colloids Surf. A, 174, 307-317 (2000).