Simultaneous purification and immobilization of Aspergillus niger xylanase on the reversibly soluble polymer Eudragit(TM) L-100

Enzyme and Microbial Technology

Volumn 27, Issue 9, 2000, Pages 672-679

  Sardar, Meryam ^a   Roy, Ipsita ^a   Gupta, Munishwar N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Cellulase free immobilized xylanase; Eudragit(TM); Non covalent immobilization of enzymes; UV Fluorescence CD spectra of immobilized proteins; Water soluble polymers; Xylanase

Indexed keywords

CATALYST ACTIVITY; ENZYME IMMOBILIZATION; FLUORESCENCE; MACROMOLECULES; MOLECULAR STRUCTURE; POLYMERS; PURIFICATION; SOLUBILITY; ULTRAVIOLET SPECTROSCOPY; WATER;

XYLANASE;

ENZYMES;

ADSORPTION; ARTICLE; ASPERGILLUS NIGER; CIRCULAR DICHROISM; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE SPECTROSCOPY; NONHUMAN; PAPER INDUSTRY; PH; ULTRAVIOLET SPECTROSCOPY;

ASPERGILLUS NIGER;

EID: 0034669586     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(00)00257-X     Document Type: Article

References (42)

1. Application of reversibly soluble-insoluble polymers in bioprocessing
2. Purification of xylanase from Trichoderma viride by precipitation with an anionic polymer Eudragit S-100
3. Use smart polymers for bioseparation
4. Properties of a reversibly soluble-insoluble cellulase and its application to repeated hydrolysis of crystalline cellulose
5. Properties and repeated use of a reversibly soluble-insoluble yeast lytic enzyme
6. Production of soluble sugar from cellulose material by repeated use of a reversibly soluble-autoprecipitating cellulase
7. A model study on Eudragit and polyethyleneimine as soluble carriers for α-amylase for repeated hydrolysis of starch
8. Non-covalent immobilization of enzymes on an enteric polymer, Eudragit S-100
9. Preparation characterization and application of Aspergillus sp. xylanase immobilized on Eudragit S-100
10. Roles for microbial enzymes in pulp and paper processing
11. Bleaching of eucalyptus kraft pulp with commercial xylanases
12. Microbial xylanases for paper industry
13. Physical characterization of enzymatically modified kraft pulp fibres
14. Treatment of recycled kraft pulps with Trichoderma reesei hemicellulases and cellulases
15. Chitosan as an affinity macroligand for precipitation of N-acetyl glucosamine binding proteins/enzymes
16. Purification of endo-polygalacturonase by affinity precipitation using alginate
17. Use of dinitrosalicylic acid reagent for determination of reducing sugar
18. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
19. Measurement of cellulase activities
20. Interlaboratory testing of methods for assay of xylanase activity
21. Affinity precipitation of extracellular microbial enzymes
22. Affinity thermoprecipitation of lactate dehydrogenase and pyruvate kinase from porcine muscle using Eudragit bound Cibacron blue
23. Purification of wheat germ agglutinin using flocculation with chitosan and a subsequent centrifugation step
24. Alginate beads as an affinity material for alpha amylases
25. Trends in affinity-based separation of proteins/enzymes
26. An experimental verification of the theory of diffusional limitation of immobilized enzyme
27. Immobilization of β-galactosidase on benzoquinone-activated beaded cellulose
28. Influence of lectin concentration on the catalytic properties of S1 nuclease bound to Concanavalin A-Sepharose
29. Immobilization of tetrameric and monomeric forms of pigeon liver malic enzyme on Sepharose beads
30. Non-covalent immobilization of potato (Solanum tuberosum) polyphenol oxidase on chitin
31. Models of enzyme deactivation
32. Purification and immobilization of Aspergillus niger pectinase on magnetic latex beads
33. Immobilization of acid phosphatase from Arachis hypogaea on CM cellulose
34. Repeated hydrolysis of raw starch using amylase immobilized on a reversibly soluble-insoluble carrier
35. Adsorption to solid surfaces
36. Fractionation and purification of endo-1,4-beta-xylanases and exo-1,4-beta-xylosidases of Aspergillus niger
37. Site-directed mutagenesis at aspartate and glutamate residues of xylanase from Bacillus pumilus
38. Measuring the conformational stability of enzymes
39. Separation of bacterial xylanase by precipitation using Eudragit S-100
40. β-1,4-xylan degrading enzyme systems. Biochemistry, molecular biology and applications