α-chymotrypsin shows higher activity in water as well as organic solvents after three phase partitioning

Biocatalysis and Biotransformation

Volumn 22, Issue 4, 2004, Pages 261-268

  Roy, Ipsita ^a   Gupta, Munishwar N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

chymotrypsin; Nonaqueous enzymology; Solvent effects; Three phase partitioning

Indexed keywords

ESTERIFICATION; ETHANOL; HYDROLYSIS; ORGANIC SOLVENTS; PHASE TRANSITIONS; SPECTROSCOPIC ANALYSIS; TOLUENE;

FLUORESCENCE EMISSION SPECTRUM; N-OCTANE; QUANTUM YIELDS; UNTREATED CONTROL;

ENZYMES;

ACETIC ACID DERIVATIVE; ALCOHOL; CHYMOTRYPSIN A; ESTER DERIVATIVE; OCTANE; ORGANIC SOLVENT; TOLUENE; WATER;

AQUEOUS SOLUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ESTERIFICATION; FLUORESCENCE; HYDROLYSIS; MICHAELIS MENTEN KINETICS; PH; PHASE PARTITIONING; QUANTUM YIELD; TEMPERATURE;

EID: 11244328195     PISSN: 10242422     EISSN: None     Source Type: Journal    
DOI: 10.1080/10242420400010523     Document Type: Article

References (37)

1. Abeles, R.H., Frey, P.A. and Jencks, W.P. (1992) Biochemistry (Jones and Bartlett Publishers, London), pp. 101-102.
2. Bornscheuer, U.T. and Pohl, C. (2001) Improved biocatalysis by directed evolution and rational protein design, Curr. Opin. Chem. Biol. 5, 137-143.
3. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-254.
4. Branden, C. and Tooze, J. (1991) Introduction to protein structure (Garland Publishing Inc, New York), pp. 238-239.
5. Brandt, K.G., Himoe, A. and Hess, G.F. (1967) Investigations of the chymotrypsin-catalyzed hydrolysis of specific substrates. III. Determination of individual rate constants and enzymesubstrate binding constants for specific amides and ester substrates, J. Biol. Chem. 242, 3973-3982.
6. Clapes, P., Caminal, G., Feliu, J.A. and López-Santín, J.A. (2000) Peptide synthesis in non-aqueous media, In: Gupta, M.N., ed, Methods in non-aqueous enzymology (Birkhauser Verlag, Basel), pp. 133-145.
7. Cornish-Bowden, A. (1995) Analysis of enzyme kinetic data (Oxford University Press, Oxford), p. 167.
8. Dekker, L.A. (1977) Chymotrypsin, In: Worthington Enzyme Manual (Worthington Biochemical Corp, New Jersey), pp. 215-220.
9. Dennsion, C. and Lovrein, R. (1997) Three phase partitioning: Concentration and purification of proteins, Protein Expr. Purif. 11, 149-161.
10. Desie, G., Boens, N. and De Schryver, F.C. (1986) Study of time-resolved tryptophan fluorescence of crystalline α -chymotrypsin, Biochemistry 25, 8301-8308.
11. Fersht, A. (1985a) Enzyme structure and mechanism (W.H. Freeman and Co, New York), pp. 103-104.
12. Fersht, A. (1985b) Enzyme structure and mechanism (W.H. Freeman and Co, New York), pp. 121-124.
13. Fujimura, M., Mori, T. and Tosa, T. (1987) Preparation and properties of soluble-insoluble immobilized proteases, Biotechnol. Bioeng. 29, 747-752.
14. Gupta, M.N. (1992) Enzyme function in organic solvents, Eur. J. Biochem. 203, 25-32.
15. Halling, P.J. (2002) Enzymic conversions in organic and other low water media, In: Drauz, K. and Waldmann, H., eds, Enzyme catalysis in organic synthesis, Vol. 1 (Wiley-VCH, Weinheim), pp. 259-285.
16. Jacobs, G.R., Pike, R.N. and Dennison, C. (1989) Isolation of Cathepsin D using three phase partitioning in t-butanol/ water/ammonium sulphate, Anal. Biochem. 180, 169-171.
17. Klibanov, A.M. (1986) Enzymes that work in organic solvents, Chemtech 6, 354-359.
18. Lee, M.Y. and Dordick, J.S. (2002) Enzyme activation for non aqueous media, Curr. Opin. Biotechnol. 13, 376-384.
19. Lovrein, R., Goldensoph, C., Anderson, P.C. and Odegaard, B. (1987) Three phase partitioning (TPP) via t-butanol: Enzymes separation from crudes, In: Burgess, R., ed, Protein purification: micro to macro (Alan R. Liss, Inc, New York), pp. 131-148.
20. Lovrein, R.E., Conroy, M.J. and Richardson, T.I. (1994) Molecular basis for protein separations, In: Gregory R.B., ed, Protein-solvent interactions (Marcel Dekker, New York), pp. 521-553.
21. Lowry, O.H., Rosebrough, N.J., Fall, A.C. and Randall, R.J. (1951) Protein measurement with the Folin Phenol reagent, J. Biol. Chem. 193, 265-275.
22. Reslow, M., Adlercreutz, P. and Mattiasson, B. (1987) Organic solvents for bioorganic synthesis. I. Optimization of parameters for a chymotrypsin catalyzed process, Appl. Microb. Biotechnol. 26, 1-8.
23. Reslow, M., Adlercreutz, P. and Mattiasson, B. (1988a) On the importance of support material for bioorganic synthesis: Influence of water partition between solvent, enzyme and solid support in water-poor reaction media, Eur. J. Biochem. 172, 573-578.
24. Reslow, M., Adlercreutz, P. and Mattiasson, B. (1988b) The influence of water on protease-catalyzed peptide synthesis in acetonitrile/water mixtures, Eur. J. Biochem. 177, 313-318.
25. Riddick, J.A., Bunger, W.B. and Sakano, T.K. (1986) Techniques of chemistry, Vol. 2 (Wiley-Interscience, New York).
26. Roy, I. and Gupta, M.N. (2002) Three phase affinity partitioning of proteins, Anal. Biochem. 300, 11-14.
27. Roy, I. and Gupta, M.N. (2004) Enhancing reaction rate for transesterification reaction catalyzed by Chromobacterium lipase Enz. Microb. Technol. (in press).
28. Singh, R.K., Gourinath, S., Sharma, S., Roy, I., Gupta, M.N., Betzel, C., Srinivasan, A. and Singh, T.P. (2001) Enhancement of enzyme activity through three phase partitioning: Crystal structure of a modified serine proteinase at 1.5Å resolution, Protein Eng. 14, 307-313.
29. Sharma, A., Sharma, S. and Gupta, M.N. (2000) Purification of alkaline phosphatase from chicken intestine by three-phase partitioning and use of Phenyl-Sepharose 6B in the batch mode, Bioseparation 9, 155-161.
30. Sharma, A. and Gupta, M.N. (2001) Purification of pectinase from tomato using three phase partitioning, Biotechnol. Lett. 23, 1625-1627.
31. Srinivasulu, S. and Acharya, A.S. (2002) Product-conformation-driven ligation of peptides by V8 protease, Protein Sci. 11, 1384-1392.
32. Teale, F.W.J. (1960) The ultraviolet fluorescence of proteins in neutral solutions, Biochem. J. 76, 381-388.
33. Walsh, G. and Headon, D. (1994) Protein Biotechnology (John Wiley & Sons, Chichester), pp. 302-336.
34. Walsh, K.A. and Wilcox, P.E. (1970) Serine proteases, In: Perlmann, G.E. and Lorand, L., eds, Methods in Enzymology, Vol. 19 (Academic Press, New York), pp. 31-41.
35. Yang, Z. and Russell, A.J. (1996) Fundamentals of nonaqueous enzymology, In: Koskinen, A.M.P. and Klibanov, A.M, eds, Enzymatic reactions in organic media (Blackie Academic and Professional, Glasgow), pp. 43-69.
36. Zaks, A. and Klibanov, A.M. (1984) Enzyme catalysis in organic media at 100°C, Science 224, 1249-1251.
37. Zaks, A. and Klibanov, A.M. (1988) Enzyme catalysis in non-aqueous solvents, J. Biol. Chem. 263, 3194-3201.