One-step purification of glucoamylase by affinity precipitation with alginate

Journal of Molecular Recognition

Volumn 14, Issue 5, 2001, Pages 295-299

  Teotia, S ^a   Lata, R ^b   Khare, S K ^a   Gupta, M N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b INDIAN AGRICULTURAL RESEARCH INSTITUTE   (India)

Author keywords

Affinity ligand; Affinity precipitation; Alginate; Aspergillus niger; Bacillus amyloliquefaciens; Glucoamylases

Indexed keywords

AFFINITY PRECIPITATION; ALGINIC ACID; BACTERIUM CULTURE; BINDING SITE; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; ENZYME PURIFICATION; GLUCAN 1,4 ALPHA GLUCOSIDASE; MALTOSE; MOLECULAR RECOGNITION; MOLECULAR WEIGHT; PRECIPITATION; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS;

ALGINATES; ALPHA-AMYLASE; ASPERGILLUS NIGER; BACILLUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUCAN 1,4-ALPHA-GLUCOSIDASE; GLUCURONIC ACID; HEXURONIC ACIDS; LIGANDS; MALTOSE;

EID: 0035151810     PISSN: 09523499     EISSN: None     Source Type: Journal    
DOI: 10.1002/jmr.540     Document Type: Article

References (24)

1. Glucoamylase of Aspergillus niger
2. Amylases α and β
3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
4. Enzymes involved in the processing of starch to sugars
5. Development of yeast strains for the efficient utilization of starch: Evaluation of constructs that express alpha-amylase and glucoamylase separately or as bifunctional fusion proteins
6. Use of dinitrosalicylic acid reagent for determination of reducing sugar
7. Affinity precipitation
8. Affinity precipitation of proteins
9. An introduction to polyacrylamide gel electrophoresis
10. Determination of β-amylase in presence of α-amylase
11. One-step hydrolysis of starch using a recombinant strain of Saccharomyces cerevisiae producing alpha-amylase, glucoamylase and pullulanase
12. Purification of recombinant protein A by aqueous two phase extraction integrated with affinity precipitation
13. Evaluation of alginate as a ligand carrier in affinity precipitation
14. Comparative studies on glucoamylases from three sources
15. Biomass conversion: Synergistic use of α-amylase and amyloglucosidase for rapid and maximum conversion of starch into glucose
16. Affinity precipitation of extra cellular microbial enzymes
17. Current trends in affinity-based separation of proteins/enzymes
18. Exploiting unusual affinity of usual polysaccharides for separation of enzymes on fluidized beds
19. Alginate beads as an affinity material for α-amylases
20. Myoinositol hexaphosphate as a potential inhibitor of α-amylases
21. Purification of wheat germ amylase by precipitation
22. Alginate as immobilization matrix for cells
23. An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate
24. Microbial amylolytic enzymes