Chitosan as a macroaffinity ligand: Purification of chitinases by affinity precipitation and aqueous two-phase extractions

Journal of Chromatography A

Volumn 1052, Issue 1-2, 2004, Pages 85-91

  Teotia, S ^a   Lata, R ^b   Gupta, M N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b INDIAN AGRICULTURAL RESEARCH INSTITUTE   (India)

Author keywords

Affinity precipitation; Chitinases; Chitosan; Polymers; Protein purification; Two phase affinity extraction

Indexed keywords

CHITOSAN; GEL ELECTROPHORESIS; LIGANDS;

DESORPTION; ELECTROPHORESIS; ENZYME KINETICS; EXTRACTION; MOLECULAR WEIGHT; POLYETHYLENE GLYCOLS; PRECIPITATION (CHEMICAL); PURIFICATION;

AMINES;

CHITINASE; CHITOSAN; MACROGOL; MAGNESIUM CHLORIDE;

ARTICLE; CABBAGE; DESORPTION; ENZYME ACTIVITY; EXTRACTION; GEL ELECTROPHORESIS; MOLECULAR WEIGHT; NEUROSPORA CRASSA; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PURIFICATION;

CHITINASE; CHITOSAN; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; LIGANDS; WATER;

BRASSICA OLERACEA VAR. CAPITATA; MARSHALLIA CAESPITOSA; NEUROSPORA; NEUROSPORA CRASSA;

EID: 4744339826     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2004.08.096     Document Type: Article

References (35)

1. M.V. Deshpande J. Sci. Ind. Res. 45 1986 277
2. J.P. Chen, and K.C. Chang J. Chem. Technol. Biotechnol. 60 1994 133
3. A.V. Ilyina, V.E. Tikhonov, V.P. Varlamov, L.A. Radigina, N.Y. Tatarinova, and I.A. Yamskov Biotechnol. Appl. Biochem. 21 1995 139
4. A.V. Ilyina, V.P. Varlamov, V.E. Tikhonov, I.A. Yamskov, and V.A. Davankov Biotechnol. Appl. Biochem. 19 1994 199
5. F. Shahidi, J.K.V. Arachchi, and Y.-J. Jeon Trends Food Sci. Technol. 10 1999 37
6. R.A.A. Muzzarelli Chitin 1977 Pergamon Press Oxford, UK
7. R. Vaidya, S. Roy, S. Macmil, S. Gandhi, P. Vyas, and H.S. Chhatpar Biotechnol. Lett. 25 2003 715
8. B. Bhusan, and G.S. Hoondal Biotechnol. Lett. 20 1998 157
9. G. Xia, C. Jin, J. Zhon, S. Yang, S. Zhang, and C. Jin Eur. J. Biochem. 268 2001 4079
10. I. Kawachi, T. Fujida, M. Ujita, Y. Ishii, K. Yamagishi, H. Sato, T. Funaguma, and A. Hara J. Biosci. Biotechnol. 92 2001 544
11. C.-J. Woo, and H.-D. Park Biotechnol. Lett. 25 2004 409
12. V.E. Tikhonov, L.A. Radigina, I.A. Yamskov, N.D. Gulyaeva, A.V. Ilyina, M.V. Anisimova, V.P. Varlamov, and N.Y. Tatarinova Enzyme Microb. Technol. 22 1998 82
13. J.G. Huddleston, and A. Lyddiatt M. Verrall Downstream Processing of Natural Products 1996 Wiley Chichester 53
14. M. Kamihira, R. Kaul, and B. Mattiasson Biotechnol. Bioeng. 40 1992 1381
15. D. Guoqiang, R. Kaul, and B. Mattiasson J. Chromatogr. A 668 1994 145
16. S. Teotia, and M.N. Gupta J. Chromatogr. A 923 2001 275
17. S. Teotia, and M.N. Gupta Protein Expr. Purif. 22 2001 484
18. S. Teotia, and M.N. Gupta J. Chromatogr. A 1025 2004 297
19. A. Sharma, S. Sharma, and M.N. Gupta Protein Expr. Purif. 18 2001 111
20. S. Teotia, R. Lata, S.K. Khare, and M.N. Gupta J. Mol. Recognit. 14 2001 295
21. S. Teotia, S.K. Khare, and M.N. Gupta Enzyme Microb. Technol. 28 2001 792
22. S. Sharma, A. Sharma, and M.N. Gupta Bioseparation 9 2000 93
23. I. Roy, and M.N. Gupta J. Chromatogr. A 998 2003 103
24. C. Senstad, and B. Mattiasson Biotechnol. Bioeng. 34 1989 389
25. R. Tyagi, A. Kumar, M. Sardar, S. Kumar, and M.N. Gupta Isol. Purif. 2 1996 217
26. A. Arroyo-Begovich Methods Enzymol. 161 1988 471
27. P. Bridge S. Doonam Methods in Molecular Biology vol. 59 1996 Humana Press Totowa, NJ
28. C.-T. Chang, H.-F. Lo, C.-J. Wu, and H.-J. Sung Biochem. Int. 28 1992 707
29. J.P. Zikakis, and J.E. Castle Methods Enzymol. 161 1988 490
30. M.N. Gupta, and B. Mattiasson G. Street Highly Selective Separations in Biotechnology 1994 Chapman and Hall London 7
31. G.L. Miller Anal. Chem. 31 1959 426
32. M.M. Bradford Anal. Biochem. 72 1976 248
33. C.H.W. Hirs Methods Enzymol. 11 1967 411
34. B.D. Hames B.D. Hames D. Rickwood Gel Electrophoresis of Proteins: A Practical Approach 1986 IRL Press Oxford 1
35. M.L. Yarmush, K.P. Antonsen, S. Sundaram, and D.M. Yarmush Biotechnol. Prog. 8 1992 168