Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded-bed adsorption

Biotechnology and Bioengineering

Volumn 67, Issue 2, 2000, Pages 206-216

  Clemmitt, R H ^a   Chase, H A ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Expanded bed adsorption; Facilitated downstream processing; Immobilized metal affinity chromatography; Metal affinity precipitation; Metal ion transfer; Polyhistidine tails

Indexed keywords

ADSORPTION ISOTHERMS; BIOREACTORS; CHROMATOGRAPHIC ANALYSIS; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; ION EXCHANGE; PRECIPITATION (CHEMICAL); PURIFICATION;

EXPANDED BED ADSORPTION (EBA); GLUTATHIONE TRANSFERASE; HISTIDINE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY (IMAC);

BIOTECHNOLOGY;

ADSORPTION; ARTICLE; BINDING AFFINITY; ESCHERICHIA COLI; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PROCESSING;

ADSORPTION; BIOTECHNOLOGY; CHELATING AGENTS; COPPER; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FERMENTATION; GLUTATHIONE TRANSFERASE; HISTIDINE; NICKEL; RECOMBINANT PROTEINS; ZINC;

EID: 0034688320     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000120)67:2<206::AID-BIT10>3.0.CO;2-X     Document Type: Article

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