Recent observations on HIV type-1 infection in the genital tract of men and women

AIDS

Volumn 17, Issue 4, 2003, Pages 455-480

  Coombs, Robert W ^a   Reichelderfer, Patricia S ^b   Landay, Alan L ^c  

^a UNIVERSITY OF WASHINGTON   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^c RUSH UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

AIDS; Cervix; Female; Genitalia; HIV 1; Male; Mucous membrane; Semen; Sexual transmission; Virus shedding

Indexed keywords

ANTIRETROVIRUS AGENT; CD4 ANTIGEN; VIRUS DNA; VIRUS RNA;

DNA DETERMINATION; GENITAL SYSTEM; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNITY; IMMUNOLOGICAL PARAMETERS; MENSTRUAL CYCLE; PHENOTYPE; PRIORITY JOURNAL; REVIEW; UTERINE CERVIX; VIROLOGY; VIRUS CULTURE; VIRUS LOAD; VIRUS SHEDDING; VIRUS TRANSMISSION;

ADOLESCENT; ADULT; ANTIRETROVIRAL THERAPY, HIGHLY ACTIVE; CERVIX UTERI; DNA, VIRAL; FEMALE; HIV ANTIBODIES; HIV INFECTIONS; HIV-1; HUMANS; LYMPHOCYTE COUNT; MALE; MENSTRUAL CYCLE; RNA, VIRAL; SEMEN; SEXUALLY TRANSMITTED DISEASES; SPECIMEN HANDLING; T-LYMPHOCYTES; VAGINAL SMEARS; VIRUS SHEDDING;

EID: 0037423819     PISSN: 02699370     EISSN: None     Source Type: Journal    
DOI: 10.1097/00002030-200303070-00001     Document Type: Review

References (287)

1. Levy JA. HIV and the Pathogenesis of AIDS, 2nd edn. Washington, DC: American Society for Microbiology; 1998.
2. Mostad SB, Kreiss JK. Shedding of HIV-1 in the genital tract. AIDS 1996; 10:1305-1315.
3. Vernazza PL, Eron JJ, Fiscus SA, Cohen MS. Sexual transmission of HIV: infectiousness and prevention. AIDS 1999; 13:155-166.
4. Chakraborty H, Sen PK, Helms RW, Vernazza PL, Fiscus SA, Eron JJ, et al. Viral burden in genital secretions determines male-to-female sexual transmission of HIV-1: a probabilistic empiric model. AIDS 2001; 15:621-627.
5. Anderson RM. Transmission dynamics of sexually transmitted infections. In: Holmes KK, Sparling PF, Mårdh P-A, Lemon SM, Stamm WE, Piot P, et al. editors. Sexually transmitted diseases, 3rd edn. New York: McGraw-Hill; 1999. Chapter 3, pp. 25-37.
6. Ho DD, Schooley RT, Rota TR, Kaplan JC, Flynn T, Salahuddin SZ, et al. HTLV-III in the semen and blood of a healthy homosexual man. Science 1984; 226:451-453.
7. Zagury D, Bernard J, Leibowitch J, Safai B, Groopman JE, Feldman M, et al. HTLV-III in cells cultured from semen of two patients with AIDS. Science 1984; 226:449-451.
8. Wofsy CB, Cohen JB, Hauer LB, Padian NS, Michaelis BA, Evans LA, et al. Isolation of AIDS-associated retrovirus from genital secretions of women with antibodies to the virus. Lancet 1986; 1:527-529.
9. Vogt MW, Witt DJ, Craven DE, Byington R, Crawford DF, Schooley RT, et al. Isolation of HTLV-III/LAV from cervical secretions of women at risk for AIDS. Lancet 1986; 1:525-527.
10. Nuovo GJ, Forde A, MacConnell P, Fahrenwald R. In situ detection of PCR-amplified HIV-1 nucleic acids and tumor necrosis factor cDNA in cervical tissues. Am J Pathol 1993; 143:40-48.
11. Hocini H, Becquart P, Bouhlal H, Chomont N, Ancuta P, Kazatchkine MD, et al. Active and selective transcytosis of cell-free human immunodeficiency virus through a tight polarized monolayer of human endometrial cells. J Virol 2001; 75:5370-5374.
12. Farrar DJ, Cu Uvin S, Caliendo AM, Costello SF, Murphy DM, Flanigan TP, et al. Detection of HIV-1 RNA in vaginal secretions of HIV-1-seropositive women who have undergone hysterectomy. AIDS 1997; 11:1296-1297.
13. Yeaman GR, White HD, Howell A, Prabhala R, Wira CR. The mucosal immune system in the human female reproductive tract: potential insights into the heterosexual transmission of HIV. AIDS Res Hum Retrovir 1998; 14 (Suppl 1):S57-62.
14. Johnstone FD, Williams AR, Bird GA, Bjornsson S. Immunohistochemical characterization of endometrial lymphoid cell populations in women infected with human immunodeficiency virus. Obstet Gynecol 1994; 83:586-593.
15. Jacobson DL, Peralta L, Farmer M, Graham NM, Wright TC, Zenilman J. Cervical ectopy and the transformation zone measured by computerized planimetry in adolescents. Int J Gynaecol Obstet 1999; 66:7-17.
16. Moss GB, Clemetson D, D'Costa L, Plummer FA, Ndinya-Achola JO, Reilly M, et al. Association of cervical ectopy with heterosexual transmission of human immunodeficiency virus: results of a study of couples in Nairobi, Kenya. J Infect Dis 1991; 164:588-591.
17. Plourde PJ, Pepin J, Agoki E, Ronald AR, Ombette J, Tyndall M, et al. Human immunodeficiency virus type 1 seroconversion in women with genital ulcers. J Infect Dis 1994; 170:313-317.
18. Kreiss J, Willerford DM, Hensel M, Emonyi W, Plummer F, Ndinya-Achola J, et al. Association between cervical inflammation and cervical shedding of human immunodeficiency virus DNA. J Infect Dis 1994; 170:1597-1601.
19. Kuhn L, Denny L, Pollack AE, Wright TC. Prevalence of visible disruption of cervical epithelium and cervical ectopy in African women using Depo-Provera. Contraception 1999; 59:363-367.
20. Crum CP. Chapter 23: Female Genital Tract. In: Cotran RS, Robbins SL, Kumar V, editors. Robbins pathologic basis of disease, 5th edn. Philadelphia: WB Saunders; 1994. pp. 1033-1088.
21. Wright TC, Ferenczy A. Chapter 5: Anatomy and histology of the cervix. In: Kurman RJ, edltor. Blaustein's pathology of the female genital tract, 5th ed. New York: Springer-Verlag; 2002.
22. Wright TC, Jr., Subbarao S, Ellerbrock TV, Lennox JL, Evans-Strickfaden T, Smith DG, et al. Human immunodeficiency virus 1 expression in the female genital tract in association with cervical inflammation and ulceration. Am J Obstet Gynecol 2001; 184:279-285.
23. Cu-Uvin SC, Anderson D, Parekh B. Human immunodeficiency virus-1 shedding in the genital tract of a female long-term nonprogressor without detectable plasma human immunodeficiency virus ribonudeic acid. Am J Obstet Gynecol 1997; 177:490-491.
24. Rasheed S. Infectivity and dynamics of HIV type 1 replication in the blood and reproductive tract of HIV type 1-infected women. AIDS Res Hum Retrovir 1998; 14 (Suppl 1):S105-118.
25. Hart CE, Lennox JL, Pratt-Palmore M, Wright TC, Schinazi RF, Evans-Strickfaden T, et al. Correlation of human immunodeficiency virus type 1 RNA levels in blood and the female genital tract. J Infect Dis 1999; 179:871-882.
26. Baron P, Bremer J, Wasserman SS, Nowicki M, Driscoll B, Polsky B, et al. Detection and quantitation of human immunodeficiency virus type 1 in the female genital tract. The Division of AIDS Treatment Research Initiative 009 Study Group. J Clin Microbiol 2000; 38:3822-3824.
27. Hajjar AM, Lewis PF, Endeshaw Y, Ndinya-Achola J, Kreiss JK, Overbaugh J. Efficient isolation of human immunodeficiency virus type 1 RNA from cervical swabs. J Clin Microbiol 1998; 36:2349-2352.
28. Iversen AK, Larsen AR, Jensen T, Fugger L, Balslev U, Wahl S, et al. Distinct determinants of human immunodeficiency virus type 1 RNA and DNA loads in vaginal and cervical secretions. J Infect Dis 1998; 177:1214-1220.
29. Mostad SB. Prevalence and correlates of HIV type 1 shedding in the female genital tract. AIDS Res Hum Retrovir 1998; 14 (Suppl 1):S11-15.
30. Coombs RW, Wright DJ, Reichelderfer PS, Burns DN, Cohn J, Cu-Uvin S, et al. Variation of human immunodeficiency virus type 1 viral RNA levels in the female genital tract: implications for applying measurements to individual women. J Infect Dis 2001; 184:1187-1191.
31. Webber MP, Schoenbaum EE, Farzadegan H, Klein RS. Tampons as a self-administered collection method for the detection and quantification of genital HIV-1. AIDS 2001; 15:1417-1420.
32. John GC, Sheppard H, Mbori-Ngacha D, Nduati R, Maron D, Reiner M, et al. Comparison of techniques for HIV-1 RNA detection and quantitation in cervicovaginal secretions. J Acquir Immune Defic Syndr 2001; 26:170-175.
33. Kozlowski PA, Lynch RM, Patterson RR, Cu-Uvin S, Flanigan TP, Neutra MR. Modified wick method using Weck-Cel sponges for collection of human rectal secretions and analysis of mucosal HIV antibody. J Acquir Immune Defic Syndr 2000; 24:297-309.
34. Rohan LC, Edwards RP, Kelly LA, Colenello KA, Bowman FP, Crowley-Nowick PA. Optimization of the Weck-Cel collection method for quantitation of cytokines in mucosal secretions. Clin Diagn Lab Immunol 2000; 7:45-48.
35. Hildesheim A, Bratti MC, Edwards RP, Schiffman M, Rodriguez AC, Herrero R, et al. Collection of cervical secretions does not adversely affect Pap smears taken immediately afterward. Clin Diagn Lab Immunol 1998; 5:491-493.
36. Reichelderfer PS, Coombs RW, Wright DJ, Cohn J, Burns DN, Cu-Uvin S, et al. Effect of menstrual cycle on HIV-1 levels in the peripheral blood and genital tract. WHS 001 Study Team. AIDS 2000; 14:2101-2107.
37. Belec L, Meillet D, Levy M, Georges A, Tevi-Benissan C, Pillot J. Dilution assessment of cervicovaginal secretions obtained by vaginal washing for immunological assays. Clin Diagn Lab Immunol 1995; 2:57-61.
38. Mohamed AS, Becquart P, Hocini H, Metais P, Kazatchkine M, Belec L. Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus. Clin Diagn Lab Immunol 1997; 4:624-626.
39. Brambilla D, Reichelderfer PS, Bremer JW, Shapiro DE, Hershow RC, Katzenstein DA, et al. The contribution of assay variation and biological variation to the total variability of plasma HIV-1 RNA measurements. The Women Infant Transmission Study Clinics. Virology Quality Assurance Program. AIDS 1999; 13:2269-2279.
40. Villanueva JM, Ellerbrock TV, Lennox JL, Bush TJ, Wright TC, Pratt-Palmore M, et al. The menstrual cycle does not affect human immunodeficiency virus type 1 levels in vaginal secretions. J Infect Dis 2002; 185:170-177.
41. Taylor S, Back DJ, Drake SM, Workman J, Reynolds H, Gibbons SE, et al. Antiretroviral drug concentrations in semen of HIV-infected men: differential penetration of indinavir, ritonavir and saquinavir. J Antimicrob Chemother 2001; 48:351-354.
42. Quayle AJ, Xu C, Mayer KH, Anderson DJ. T lymphocytes and macrophages, but not motile spermatozoa, are a significant source of human immunodeficiency virus in semen. J Infect Dis 1997; 176:960-968.
43. Dejucq N, Jegou B. Viruses in the mammalian male genital tract and their effects on the reproductive system. Microbiol Mol Biol Rev 2001; 65:208-231.
44. Brogi A, Presentini R, Solazzo D, Piomboni P, Costantino-Ceccarini E. Interaction of human immunodeficiency virus type 1 envelope glycoprotein gp120 with a galactoglycerolipid associated with human sperm. AIDS Res Hum Retrovir 1996; 12:483-489.
45. Scofield VL. Sperm as infection-potentiating cofactors in HIV transmission. J Reprod Immunol 1998; 41:359-372.
46. Zhu T, Wang N, Carr A, Nam DS, Moor-Jankowski R, Cooper DA, et al. Genetic characterization of human immunodeficiency virus type 1 in blood and genital secretions: evidence for viral compartmentalization and selection during sexual transmission. J Virol 1996; 70:3098-3107.
47. Ball SC. Addressing the issue of childbearing in heterosexual couples discordant for HIV. AIDS Read 2000; 10:144-145.
48. Chrystie IL, Mullen JE, Braude PR, Rowell P, Williams E, Elkington N, et al. Assisted conception in HIV discordant couples: evaluation of semen processing techniques in reducing HIV viral load. J Reprod Immunol 1998; 41:301-306.
49. Gilling-Smith C. HIV prevention. Assisted reproduction in HIV-discordant couples. AIDS Read 2000; 10:581-587.
50. Hanabusa H, Kuji N, Kato S, Tagami H, Kaneko S, Tanaka H, et al. An evaluation of semen processing methods for eliminating HIV-1. AIDS 2000; 14:1611-1616.
51. Jamieson DJ, Schieve L, Duerr A. Semen processing for HIV-discordant couples. Am J Obstet Gynecol 2001; 185:1433-1434.
52. Kim LU, Johnson MR, Barton S, Nelson MR, Sontag G, Smith JR, et al. Evaluation of sperm washing as a potential method of reducing HIV transmission in HIV-discordant couples wishing to have children. AIDS 1999; 13:645-651.
53. Marina S, Marina F, Alcolea R, Exposito R, Huguet J, Nadal J, et al. Human immunodeficiency virus type 1-serodiscordant couples can bear healthy children after undergoing intrauterine insemination. Fertil Steril 1998; 70:35-39.
54. Pasquier C, Daudin M, Righi L, Berges L, Thauvin L, Berrebi A, et al. Sperm washing and virus nucleic acid detection to reduce HIV and hepatitis C virus transmission in serodiscordant couples wishing to have children. AIDS 2000; 14:2093-2099.
55. Semprini AE, Flore S, Pardi G. Reproductive counselling for HIV-discordant couples. Lancet 1997; 349:1401-1402.
56. Weigel MM, Gentili M, Beichert M, Friese K, Sonnenberg-Schwan U. Reproductive assistance to HIV-discordant couples-the German approach. Eur J Med Res 2001; 6:259-262.
57. Brechard N, Galea P, Silvy F, Amram M, Chermann JC. [Study of HIV localization in sperm]. Contracept Fertil Sex 1997; 25:389-391.
58. Coombs RW, Speck CE, Hughes JP, Lee W, Sampoleo R, Ross SO, et al. Association between culturable human immunodeficiency virus type 1 (HIV-1) in semen and HIV-1 RNA levels in semen and blood: evidence for compartmentalization of HIV-1 between semen and blood. J Infect Dis 1998; 177:320-330.
59. Gupta P, Leroux C, Patterson BK, Kingsley L, Rinaldo C, Ding M, et al. Human immunodeficiency virus type 1 shedding pattern in semen correlates with the compartmentalization of viral Quasi species between blood and semen. J Infect Dis 2000; 182:79-87.
60. Krieger JN, Coombs RW, Collier AC, Ho DD, Ross SO, Zeh JE, et al. Intermittent shedding of human immunodeficiency virus in semen: implications for sexual transmission. J Urol 1995; 154:1035-1040.
61. Miller CJ, Vogel P, Alexander NJ, Dandekar S, Hendrickx AG, Marx PA. Pathology and localization of simian immunodeficiency virus in the reproductive tract of chronically infected male rhesus macaques. Lab Invest 1994; 70:255-262.
62. Anderson DJ, Politch JA, Martinez A, Van Voorhis BJ, Padian NS, O'Brien TR. White blood cells and HIV-1 in semen from vasectomised seropositive men. Lancet 1991; 338:573-574.
63. Ilaria G, Jacobs JL, Polsky B, Koll B, Baron P, MacLow C, et al. Detection of HIV-1 DNA sequences in pre-ejaculatory fluid. Lancet 1992; 340:1469.
64. Krieger JN, Nirapathpongporn A, Chaiyaporn M, Peterson G, Nikolaeva I, Akridge R, et al. Vasectomy and human immunodeficiency virus type 1 in semen. J Urol 1998; 159:820-825; discussion 825-826.
65. Pudney J, Oneta M, Mayer K, Seage G, 3rd, Anderson D. Pre-ejaculatory fluid as potential vector for sexual transmission of HIV-1. Lancet 1992; 340:1470.
66. Bremer J, Nowicki M, Beckner S, Brambilla D, Cronin M, Herman S, et al. Comparison of two amplification technologies for detection and quantitation of human immunodeficiency virus type 1 RNA in the female genital tract. Division of AIDS Treatment Research Initiative 009 Study Team. J Clin Microbiol 2000; 38:2665-2669.
67. Fiscus SA, Brambilla D, Coombs RW, Yen-Lieberman B, Bremer J, Kovacs A, et al. Multicenter evaluation of methods to quantitate human immunodeficiency virus type 1 RNA in seminal plasma. J Clin Microbiol 2000; 38:2348-2353.
68. Leruez-Ville M, Dulioust E, Costabliola D, Salmon D, Tachet A, Finkielsztejn L, et al. Decrease in HIV-1 seminal shedding in men receiving highly active antiretroviral therapy: an 18 month longitudinal study (ANRS EP012). AIDS 2002; 16:486-488.
69. Vernazza PL, Eron JJ, Fiscus SA. Sensitive method for the detection of infectious HIV in semen of seropositive individuals. J Virol Methods 1996; 56:33-40.
70. Kovacs A, Wasserman SS, Burns D, Wright DJ, Cohn J, Landay A, et al. Determinants of HIV-1 shedding in the genital tract of women. Lancet 2001; 358:1593-1601.
71. Saracino A, Di Stefano M, Fiore JR, Lepera A, Raimondi D, Angarano G, et al. Frequent detection of HIV-1 RNA but low rates of HIV-1 isolation in cervicovaginal secretions from infected women. New Microbiol 2000; 23:79-83.
72. Ellerbrock TV, Lennox JL, Clancy KA, Schinazi RF, Wright TC, Pratt-Palmore M, et al. Cellular replication of human immunodeficiency virus type 1 occurs in vaginal secretions. J Infect Dis 2001; 184:28-36.
73. Spinillo A, Zara F, De Santolo A, Brerra R, Maserati R, Romero E, et al. Quantitative assessment of cell-associated and cell-free virus in cervicovaginal samples of HIV-1-infected women. Clin Microbiol Infect 1999; 5:605-611.
74. Mayer KH, Boswell S, Goldstein R, Lo W, Xu C, Tucker L, et al. Persistence of human immunodeficiency virus in semen after adding indinavir to combination antiretroviral therapy. Clin Infect Dis 1999; 28:1252-1259.
75. Rasheed S, Li Z, Xu D. Human immunodeficiency virus load. Quantitative assessment in semen from seropositive individuals and in spiked seminal plasma. J Reprod Med 1995; 40:747-757.
76. Vernazza PL, Troiani L, Flepp MJ, Cone RW, Schock J, Roth F, et al. Potent antiretroviral treatment of HIV-infection results in suppression of the seminal shedding of HIV. The Swiss HIV Cohort Study. AIDS 2000; 14:117-121.
77. Kovacs A, Chan LS, Chen ZC, Meyer WA, 3rd, Muderspach L, Young M, et al. HIV-1 RNA in plasma and genital tract secretions in women infected with HIV-1. J Acquir Immune Defic Syndr 1999; 22:124-131.
78. Si-Mohamed A, Kazatchkine MD, Heard I, Goujon C, Prazuck T, Aymard G, et al. Selection of drug-resistant variants in the female genital tract of human immunodeficiency virus type 1-infected women receiving antiretroviral therapy. J Infect Dis 2000; 182:112-122.
79. Shepard RN, Schock J, Robertson K, Goujon C, Prazuck T, Aymard G, et al. Quantitation of human immunodeficiency virus type 1 RNA in different biological compartments. J Clin Microbiol 2000; 38:1414-1418.
80. Tachet A, Dulioust E, Salmon D, De Almeida M, Rivalland S, Finkielsztejn L, et al. Detection and quantification of HIV-1 in semen: identification of a subpopulation of men at high potential risk of viral sexual transmission. AIDS 1999; 13:823-831.
81. Dyer JR, Gilliam BL, Eron JJ, Jr., Grosso L, Cohen MS, Fiscus SA. Quantitation of human immunodeficiency virus type 1 RNA in cell free seminal plasma: comparison of NASBA with Amplicor reverse transcription- PCR amplification and correlation with quantitative culture. J Virol Methods 1996; 60:161-170.
82. Holodniy M, Anderson D, Wright D, Sharma O, Cohn J, Alexander N, et al. HIV quantitation in spiked vaginocervical secretions: lack of non-specific inhibitory factors. DATRI 005 Study Team. Division of AIDS Treatment Research Initiative. J Virol Methods 1998; 72:185-195.
83. Sutthent R, Chaisilwattana P, Roongpisuthipong A, Wirachsilp P, Samrangsarp K, Chaiyakul P, et al. Shedding of HIV-1 subtype E in semen and cervico-vaginal fluid. J Med Assoc Thai 1997; 80:348-357.
84. Ball JK, Curran R, Irving WL, Dearden AA. HIV-1 in semen: determination of proviral and viral titres compared to blood, and quantification of semen leukocyte populations. J Med Virol 1999; 59:356-363.
85. Xu C, Politch JA, Tucker L, Mayer KH, Seage GR, 3rd, Anderson DJ. Factors associated with increased levels of human immunodeficiency virus type 1 DNA in semen. J Infect Dis 1997; 176:941-947.
86. Clemetson DB, Moss GB, Willerford DM, Hensel M, Emonyi W, Holmes KK, et al. Detection of HIV DNA in cervical and vaginal secretions. Prevalence and correlates among women in Nairobi, Kenya. JAMA 1993; 269:2860-2864.
87. Vernazza PL, Dyer JR, Fiscus SA, Eron JJ, Cohen MS. HIV-1 viral load in blood, semen and saliva. AIDS 1997; 11:1058-1059.
88. Quesnel A, Cu-Uvin S, Murphy D, Ashley RL, Flanigan T, Neutra MR. Comparative analysis of methods for collection and measurement of immunoglobulins in cervical and vaginal secretions of women. J Immunol Methods 1997; 202:153-161.
89. Musey L, Hu Y, Eckert L, Christensen M, Karchmer T, McElrath MJ. HIV-1 induces cytotoxic T lymphocytes in the cervix of infected women. J Exp Med 1997; 185:293-303.
90. Shacklett BL, Cu-Uvin S, Beadle TJ, Pace CA, Fast NM, Donahue SM, et al. Quantification of HIV-1-specific T-cell responses at the mucosal cervicovaginal surface. AIDS 2000; 14:1911-1915.
91. Belec L, Dupre T, Prazuck T, Tevi-Benissan C, Kanga JM, Pathey O, et al. Cervicovaginal overproduction of specific IgG to human immunodeficiency virus (HIV) contrasts with normal or impaired IgA local response in HIV infection. J Infect Dis 1995; 172:691-697.
92. Lu FX. Predominate HIV1-specific IgG activity in various mucosal compartments of HIV1-infected individuals. Clin Immunol 2000; 97:59-68.
93. Raux M, Finkielsztejn L, Salmon-Ceron D, Bouchez H, Excler JL, Dulioust E, et al. IgG subclass distribution in serum and various mucosal fluids of HIV type 1-infected subjects. AIDS Res Hum Retrovir 2000; 16:583-594.
94. Battle-Miller K, Eby CA, Landay AL, Cohen MH, Sha BE, Baum LL. Antibody-dependent cell-mediated cytotoxicity in cervical lavage fluids of human immunodeficiency virus type 1-infected women. J Infect Dis 2002; 185:439-447.
95. Bardeguez AD, Skurnick JH, Perez G, Colon JM, Kloser P, Denny TN. Lymphocyte shedding from genital tract of human immunodeficiency virus-infected women: immunophenotypic and clinical correlates. Am J Obstet Gynecol 1997; 176:158-165.
96. Givan AL, White HD, Stern JE, Colby E, Gosselin EJ, Guyre PM, et al. Flow cytometric analysis of leukocytes in the human female reproductive tract: comparison of fallopian tube, uterus, cervix, and vagina. Am J Reprod Immunol 1997; 38:350-359.
97. Patterson BK, Landay A, Andersson J, Brown C, Behbahani H, Jiyamapa D, et al. Repertoire of chemokine receptor expression in the female genital tract: implications for human immunodeficiency virus transmission. Am J Pathol 1998; 153:481-490.
98. Hladik F, Lentz G, Delpit E, McElroy A, McElrath MJ. Coexpression of CCR5 and IL-2 in human genital but not blood T cells: implications for the ontogeny of the CCR5+ Th1 phenotype. J Immunol 1999; 163:2306-2313.
99. Anderson DJ, Politch JA, Tucker LD, Fichorova R, Haimovici F, Tuomala RE, et al. Quantitation of mediators of inflammation and immunity in genital tract secretions and their relevance to HIV type 1 transmission. AIDS Res Hum Retrovir 1998; 14 (Suppl 1):S43-49.
100. Belec L, Tevi-Benissan C, Lu XS, Prazuck T, Pillot J. Local synthesis of IgG antibodies to HIV within the female and male genital tracts during asymptomatic and pre-AIDS stages of HIV infection. AIDS Res Hum Retrovir 1995; 11:719-729.
101. Quayle AJ, Xu C, Mayer KH, Anderson DJ. T lymphocytes and macrophages, but not motile spermatozoa, are a significant source of human immunodeficiency virus in semen. J Infect Dis 1997; 176:960-968.
102. Raux M, Finkielsztejn L, Salmon-Ceron D, Bouchez H, Excler JL, Dulioust E, et al. Comparison of the distribution of IgG and IgA antibodies in serum and various mucosal fluids of HIV type 1-infected subjects. AIDS Res Hum Retrovir 1999; 15:1365-1376.
103. Quayle AJ, Coston WM, Trocha AK, Kalams SA, Mayer KH, Anderson DJ. Detection of HIV-1-specific CTLs in the semen of HIV-infected individuals. J Immunol 1998; 161:4406-4410.
104. Hildesheim A, McShane LM, Schiffman M, Bratti MC, Rodriguez AC, Herrero R, et al. Cytokine and immunoglobulin concentrations in cervical secretions: reproducibility of the Weck-Cel collection instrument and correlates of immune measures. J Immunol Methods 1999; 225:131-143.
105. Schacker T. The role of HSV in the transmission and progression of HIV. Herpes 2001; 8:46-49.
106. Auvert B, Buve A, Ferry B, Carael M, Morison L, Lagarde E, et al. Ecological and individual level analysis of risk factors for HIV infection in four urban populations in sub-Saharan Africa with different levels of HIV infection. AIDS 2001; 15 (Suppl 4):S15-30.
107. Palu G, Benetti L, Calistri A. Molecular basis of the interactions between herpes simplex viruses and HIV-1. Herpes 2001; 8:50-55.
108. Mercader M, Nickoloff BJ, Foreman KE. Induction of human immunodeficiency virus 1 replication by human herpesvirus 8. Arch Pathol Lab Med 2001; 125:785-789.
109. Rottingen JA, Cameron DW, Garnett GP. A systematic review of the epidemiologlc interactions between classic sexually transmitted diseases and HIV: how much really is known? Sex Transm Dis 2001; 28:579-597.
110. O'Farrell N. Enhanced efficiency of female-to-male HIV transmission in core groups in developing countries: the need to target men. Sex Transm Dis 2001; 28:84-91.
111. Sobel JD. Gynecologic infections in human immunodeficiency virus-infected women. Clin Infect Dis 2000; 31:1225-1233.
112. Cu-Uvin S, Hogan JW, Warren D, Klein RS, Peipert J, Schuman P, et al. Prevalence of lower genital tract infections among human immunodeficiency virus (HIV)-seropositive and high-risk HIV-seronegative women. HIV Epidemiology Research Study Group. Clin Infect Dis 1999; 29:1145-1150.
113. Eckert LO, Watts DH, Koutsky LA, Hawes SE, Stevens CE, Kuypers J, et al. A matched prospective study of human immunodeficiency virus serostatus, human papillomavirus DNA, and cervical lesions detected by cytology and colposcopy. Infect Dis Obstet Gynecol 1999; 7:158-164.
114. Kuhn L, Sun XW, Wright TC, Jr. Human immunodeficiency virus infection and female lower genital tract malignancy. Curr Opin Obstet Gynecol 1999; 11:35-39.
115. Torrisi A, Del Mistro A, Onnis GL, Merlin F, Bertorelle R, Minucci D. Colposcopy, cytology and HPV-DNA testing in HIV-positive and HIV- negative women. Eur J Gynaecol Oncol 2000; 21:168-172.
116. Volkow P, Rubi S, Lizano M, Carrillo A, Vilar-Compte D, Garcia-Carranca A, et al. High prevalence of oncogenic human papillomavirus in the genital tract of women with human immunodeficiency virus. Gynecol Oncol 2001; 82:27-31.
117. Greenblatt RM, Bacchetti P, Barkan S, Augenbraun M, Silver S, Delapenha R, et al. Lower genital tract infections among HIV-infected and high-risk uninfected women: findings of the Women's Interagency HIV Study (WIHS). Sex Transm Dis 1999; 26:143-151.
118. Mbopi-Keou FX, Gresenguet G, Mayaud P, Weiss HA, Gopal R, Matta M, et al. Interactions between herpes simplex virus type 2 and human immunodeficiency virus type 1 infection in African women: opportunities for intervention. J Infect Dis 2000; 182:1090-1096.
119. Kilmarx PH, Mock PA, Levine WC. Effect of Chlamydia trachomatis coinfection on HIV shedding in genital tract secretions. Sex Transm Dis 2001; 28:347-348.
120. Rotchford K, Strum AW, Wilkinson D. Effect of coinfection with STDs and of STD treatment on HIV shedding in genital-tract secretions: systematic review and data synthesis. Sex Transm Dis 2000; 27:243-248.
121. Mayaud P, Gill DK, Weiss HA, Uledi E, Kopwe L, Todd J, et al. The interrelation of HIV, cervical human papillomavirus, and neoplasia among antenatal clinic attenders in Tanzania. Sex Transm Infect 2001; 77:248-254.
122. Sewankambo N, Gray RH, Wawer MJ, Paxton L, McNaim D, Wabwire-Mangen F, et al. HIV-1 infection associated with abnormal vaginal flora morphology and bacterial vaginosis. Lancet 1997; 350:546-550.
123. Wang CC, Reilly M, Kreiss JK. Risk of HIV infection in oral contraceptive pill users: a meta-analysis. J Acquir Immune Defic Syndr 1999; 21:51-58.
124. Halperin DT. Dry sex practices and HIV infection in the Dominican Republic and Haiti. Sex Transm Infect 1999; 75:445-446.
125. Brown JE, Brown RC. Traditional intravaginal practices and the heterosexual transmission of disease: a review. Sex Transm Dis 2000; 27:183-187.
126. John GC, Nduati RW, Mbori-Ngacha D, Overbaugh J, Welch M, Richardson BA, et al. Genital shedding of human immunodeficiency virus type 1 DNA during pregnancy: association with immunosuppression, abnormal cervical or vaginal discharge, and severe vitamin A deficiency. J Infect Dis 1997; 175:57-62.
127. Mostad SB, Overbaugh J, DeVange DM, Welch MJ, Chohan B, Mandaliya K, et al. Hormonal contraception, vitamin A deficiency, and other risk factors for shedding of HIV-1 infected cells from the cervix and vagina. Lancet 1997; 350:922-927.
128. Ghys PD, Fransen K, Diallo MO, Ettiegne-Traore V, Coulibaly IM, Yeboue KM, et al. The associations between cervicovaginal HIV shedding, sexually transmitted diseases and immunosuppression in female sex workers in Abidjan, Cote d'Ivoire. AIDS 1997; 11:F85-93.
129. McClelland RS, Wang CC, Mandaliya K, Overbaugh J, Reiner MT, Panteleeff DD, et al. Treatment of cervicitis is associated with decreased cervical shedding of HIV-1. AIDS 2001; 15:105-110.
130. Wang CC, McClelland RS, Reilly M, Overbaugh J, Emery SR, Mandaliya K, et al. The effect of treatment of vaginal infections on shedding of human immunodeficiency virus type 1. J Infect Dis 2001; 183:1017-1022.
131. Clarke LM, Duerr A, Feldman J, Sierra MF, Daidone BJ, Landesman SH. Factors associated with cytomegalovirus infection among human immunodeficiency virus type 1-seronegative and -seropositive women from an urban minority community. J Infect Dis 1996; 173:77-82.
132. Mostad SB, Kreiss JK, Ryncarz A, Chohan B, Mandaliya K, Ndinya-Achola J, et al. Cervical shedding of herpes simplex virus and cytomegalovirus throughout the menstrual cycle in women infected with human immunodeficiency virus type 1. Am J Obstet Gynecol 2000; 183:948-955.
133. Greenblatt RM, Jacobson LP, Levine AM, Melnick S, Anastos K, Cohen M, et al. Human herpesvirus 8 infection and Kaposi's sarcoma among human immunodeficiency virus-infected and -uninfected women. J Infect Dis 2001; 183:1130-1134.
134. Wales NM, Nordin AJ, Newell AN, Smith JR, Barton SE, Nelson MR. Cytomegalovirus infection in the genital tract of HIV-seropositive women. AIDS 1996; 10:802-803.
135. Whitby D, Smith NA, Matthews S, O'Shea S, Sabin CA, Kulasegaram R, et al. Human herpesvirus 8: seroepidemiology among women and detection in the genital tract of seropositive women. J Infect Dis 1999; 179:234-236.
136. Rezza G, Capobianchi M, Serraino D, Peroni M, Piselli P, Calcaterra S, et al. HHV-8 shedding among HIV-infected women. J Acquir Immune Defic Syndr 2001; 28:103-104.
137. Lawn SD, Subbarao S, Wright TC, Jr, Evans-Strickfaden T, Ellerbrock TV, Lennox JL, et al. Correlation between human immunodeficiency virus type 1 RNA levels in the female genital tract and immune activation associated with ulceration of the cervix. J Infect Dis 2000; 181:1950-1956.
138. Spear GT, al-Harthi L, Sha B, Saarloos MN, Hayden M, Massad LS, et al. A potent activator of HIV-1 replication is present in the genital tract of a subset of HIV-1-infected and uninfected women. AIDS 1997; 11:1319-1326.
139. Al-Harthi L, Roebuck KA, Olinger GG, Landay A, Sha BE, Hashemi FB, et al. Bacterial vaginosis-associated microflora isolated from the female genital tract activates HIV-1 expression. J Acquir Immune Defic Syndr 1999; 21:194-202.
140. Olinger GG, Hashemi FB, Sha BE, Spear GT. Association of indicators of bacterial vaginosis with a female genital tract factor that induces expression of HIV-1. AIDS 1999; 13:1905-1912.
141. Hashemi FB, Ghassemi M, Roebuck KA, Spear GT. Activation of human immunodeficiency virus type 1 expression by Gardnerella vaginalis. J Infect Dis 1999; 179:924-930.
142. Simoes JA, Hashemi FB, Aroutcheva AA, Heimler I, Spear GT, Shott S, et al. Human immunodeficiency virus type 1 stimulatory activity by Gardnerella vaginalis: relationship to biotypes and other pathogenic characteristics. J Infect Dis 2001; 184:22-27.
143. Hashemi FB, Ghassemi M, Faro S, Aroutcheva A, Spear GT. Induction of human immunodeficiency virus type 1 expression by anaerobes associated with bacterial vaginosis. J Infect Dis 2000; 181:1574-1580.
144. Hashemi FB, Mollenhauer J, Madsen LD, Sha BE, Nacken W, Moyer MB, et al. Myeloid-related protein (MRP)-8 from cervico-vaginal secretions activates HIV replication. AIDS 2001; 15:441-449.
145. Al-Harthi L, Spear GT, Hashemi FB, Landay A, Sha BE, Roebuck KA. A human immunodeficiency virus (HIV)-inducing factor from the female genital tract activates HIV-1 gene expression through the kappaB enhancer. J Infect Dis 1998; 178:1343-1351.
146. Krieger JN, Coombs RW, Collier AC, Ross SO, Speck C, Corey L. Seminal shedding of human immunodeficiency virus type 1 and human cytomegalovirus: evidence for different immunologic controls. J Infect Dis 1995; 171:1018-1022.
147. Speck CE, Coombs RW, Koutsky LA, Zeh J, Ross SO, Hooton TM, et al. Risk factors for HIV-1 shedding in semen. Am J Epidemiol 1999; 150:622-631.
148. Cohen MS, Hoffman IF, Royce RA, Kazembe P, Dyer JR, Daly CC, et al. Reduction of concentration of HIV-1 in semen after treatment of urethritis: implications for prevention of sexual transmission of HIV- 1. AIDSCAP Malawi Research Group. Lancet 1997; 349:1868-1873.
149. Dyer JR, Eron JJ, Hoffman IF, Kazembe P, Vernazza PL, Nkata E, et al. Association of CD4 cell depletion and elevated blood and seminal plasma human immunodeficiency virus type 1 (HIV-1) RNA concentrations with genital ulcer disease in HIV-1-infected men in Malawi. J Infect Dis 1998; 177:224-227.
150. Dyer JR, Kazembe P, Vernazza PL, Gilliam BL, Maida M, Zimba D, et al. High levels of human immunodeficiency virus type 1 in blood and semen of seropositive men in sub-Saharan Africa. J Infect Dis 1998; 177:1742-1746.
151. Hobbs MM, Kazembe P, Reed AW, Miller WC, Nkata E, Zimba D, et al. Trichomonas vaginalis as a cause of urethritis in Malawian men. Sex Transm Dis 1999; 26:381-387.
152. Ping LH, Cohen MS, Hoffman I, Vernazza P, Seillier-Moiseiwitsch F, Chakraborty H, et al. Effects of genital tract inflammation on human immunodeficiency virus type 1 V3 populations in blood and semen. J Virol 2000; 74:8946-8952.
153. Sadiq ST, Taylor S, Kaye S, Bennett J, Johnstone R, Byrne P, et al. The effects of antiretroviral therapy on HIV-1 RNA loads in seminal plasma in HIV-positive patients with and without urethritis. AIDS 2002; 16:219-225.
154. Winter AJ, Taylor S, Workman J, White D, Ross JD, Swan AV, et al. Asymptomatic urethritis and detection of HIV-1 RNA in seminal plasma. Sex Transm Infect 1999; 75:261-263.
155. Ho JL, He S, Hu A, Geng J, Basile FG, Almeida MG, et al. Neutrophils from human immunodeficiency virus (HIV)-seronegative donors induce HIV replication from HIV-infected patients' mononuclear cells and cell lines: an in vitro model of HIV transmission facilitated by Chlamydia trachomatis. J Exp Med 1995; 181:1493-1505.
156. Sturm-Ramirez K, Gaye-Diallo A, Eisen G, Mboup S, Kanki PJ. High levels of tumor necrosis factor-alpha and interleukin-1beta in bacterial vaginosis may increase susceptibility to human immunodeficiency virus. J Infect Dis 2000; 182:467-473.
157. Montano MA, Nixon CP, Ndung'u T, Bussmann H, Novitsky VA, Dickman D, et al. Elevated tumor necrosis factor-alpha activation of human immunodeficiency virus type 1 subtype C in Southern Africa is associated with an NF-kappaB enhancer gain-of-function. J Infect Dis 2000; 181:76-81.
158. Cohen CR, Plummer FA, Mugo N, Maclean I, Shen C, Bukusi EA, et al. Increased interleukin-10 in the endocervical secretions of women with non-ulcerative sexually transmitted diseases: a mechanism for enhanced HIV-1 transmission? AIDS 1999; 13:327-332.
159. Olaitan A, Johnson MA, Reid WM, Poulter LW. Changes to the cytokine microenvironment in the genital tract mucosa of HIV+women. Clin Exp Immunol 1998; 112:100-104.
160. Cohn MA, Frankel SS, Rugpao S, Young MA, Willett G, Tovanabutra S, et al. Chronic inflammation with increased human immunodeficiency virus (HIV) RNA expression in the vaginal epithelium of HIV-infected Thai women. J Infect Dis 2001; 184:410-417.
161. Baeten JM, Mostad SB, Hughes MP, Overbaugh J, Bankson DD, Mandaliya K, et al. Selenium deficiency is associated with shedding of HIV-1-infected cells in the female genital tract. J Acquit Immune Defic Syndr 2001; 26:360-364.
162. French AL, Cohen MH, Gange SJ, Burger H, Gao W, Semba RD, et al. Vitamin A deficiency and genital viral burden in women infected with HIV-1. Lancet 2002; 359:1210-1212.
163. Baeten JM, McClelland RS, Overbaugh J, Richardson BA, Emery S, Lavreys L, et al. Vitamin A supplementation and human immunodeficiency virus type 1 shedding in women: results of a randomized clinical trial. J Infect Dis 2002; 185:1187-1191.
164. Liuzzi G, Chirianni A, Clementi M, Bagnarelli P, Valenza A, Cataldo PT, et al. Analysis of HIV-1 load in blood, semen and saliva: evidence for different viral compartments in a cross-sectional and longitudinal study. AIDS 1996; 10:F51-56.
165. Gupta P, Mellors J, Kingsley L, Riddler S, Singh MK, Schreiber S, et al. High viral load in semen of human immunodeficiency virus type 1- infected men at all stages of disease and its reduction by therapy with protease and nonnucleoside reverse transcriptase inhibitors. J Virol 1997; 71:6271-6275.
166. Vernazza PL, Gilliam BL, Dyer J, Fiscus SA, Eron JJ, Frank AC, et al. Quantification of HIV in semen: correlation with antiviral treatment and immune status. AIDS 1997; 11:987-993.
167. Vernazza PL, Gilliam BL, Flepp M, Dyer JR, Frank AC, Fiscus SA, et al. Effect of antiviral treatment on the shedding of HIV-1 in semen. AIDS 1997; 11:1249-1254.
168. Dyer JR, Gilliam BL, Eron JJ, Jr., Cohen MS, Fiscus SA, Vernazza PL. Shedding of HIV-1 in semen during primary infection. AIDS 1997; 11:543-545.
169. Dulioust E, Tachet A, De Almeida M, Finkielsztejn L, Rivalland S, Salmon D, et al. Detection of HIV-1 in seminal plasma and seminal cells of HIV-1 seropositive men. J Reprod Immunol 1998; 41:27-40.
170. Kiessling AA, Fitzgerald LM, Zhang D, Chhay H, Brettler D, Eyre RC, et al. Human immunodeficiency virus in semen arises from a genetically distinct virus reservoir. AIDS Res Hum Retrovir 1998; 14 (Suppl 1):S33-41.
171. Rasheed S, Li Z, Xu D, Kovacs A. Presence of cell-free human immunodeficiency virus in cervicovaginal secretions is independent of viral load in the blood of human immunodeficiency virus-infected women. Am J Obstet Gynecol 1996; 175:122-129.
172. Cu-Uvin S, Caliendo AM, Reinert S, Chang A, Juliano-Remollino C, Flanigan TP, et al. Effect of highly active antiretroviral therapy on cervicovaginal HIV-1 RNA. AIDS 2000; 14:415-421.
173. Loussert-Ajaka I, Mandelbrot L, Delmas MC, Bastian H, Benifla JL, Farfara I, et al. HIV-1 detection in cervicovaginal secretions during pregnancy. AIDS 1997; 11:1575-1581.
174. Goulston C, McFarland W, Katzenstein D. Human immunodeficiency virus type 1 RNA shedding in the female genital tract. J Infect Dis 1998; 177:1100-1103.
175. Spinillo A, Debiaggi M, Zara F, Maserati R, Polatti F, De Santolo A. Factors associated with nucleic acids related to human immunodeficiency virus type 1 in cervico-vaginal secretions. BJOG 2001; 108:634-641.
176. Spinillo A, Debiaggi M, Zara F, De Santolo A, Polatti F, Filice G. Human immunodeficiency virus type 1-related nucleic acids and papillomavirus DNA in cervicovaginal secretions of immunodeficiency virus-infected women. Obstet Gynecol 2001; 97:999-1004.
177. Debiaggi M, Zara F, Spinillo A, De Santolo A, Maserati R, Bruno R, et al. Viral excretion in cervicovaginal secretions of HIV-1-infected women receiving antiretroviral therapy. Eur J Clin Microbiol Infect Dis 2001; 20:91-96.
178. Gunthard HF, Havlir DV, Fiscus S, Zhang ZQ, Eron J, Mellors J, et al. Residual human immunodeficiency virus (HIV) Type 1 RNA and DNA in lymph nodes and HIV RNA in genital secretions and in cerebrospinal fluid after suppression of viremia for 2 years. J Infect Dis 2001; 183:1318-1327.
179. Fiore JR, Di Stefano M, Lepera A, Saracino A, Monno L, Angarano G, et al. Evidence for a local synthesis of beta-chemokines within the genital tract of both HIV-1-infected and uninfected women. J Acquir Immune Defic Syndr 1999; 21:255-257.
180. Mazzoli S, Lopalco L, Salvi A, Trabattoni D, Lo Caputo S, Semplici F, et al. Human immunodeficiency virus (HIV)-specific IgA and HIV neutralizing activity in the serum of exposed seronegative partners of HIV-seropositive persons. J Infect Dis 1999; 180:871-875.
181. Ghys PD, Belec L, Diallo MO, Ettiegne-Traore V, Becquart P, Maurice C, et al. Cervicovaginal anti-HIV antibodies in HIV-seronegative female sex workers in Abidjan, Cote d'Ivoire. AIDS 2000; 14:2603-2608.
182. Clerici M, Salvi A, Trabattoni D, Lo Caputo S, Semplici F, Biasin M, et al. A role for mucosal immunity in resistance to HIV infection. Immunol Lett 1999; 66:21-25.
183. Mazzoli S, Trabattoni D, Lo Caputo S, Piconi S, Ble C, Meacci F, et al. HIV-specific mucosal and cellular immunity in HIV-seronegative partners of HIV-seropositive individuals. Nat Med 1997; 3:1250-1257.
184. Devito C, Broliden K, Kaul R, Svensson L, Johansen K, Kiama P, et al. Mucosal and plasma IgA from HIV-1-exposed uninfected individuals inhibit HIV-1 transcytosis across human epithelial cells. J Immunol 2000; 165:5170-5176.
185. Devito C, Hinkula J, Kaul R, Lopalco L, Bwayo JJ, Plummet F, et al. Mucosal and plasma IgA from HIV-exposed seronegative individuals neutralize a primary HIV-1 isolate. AIDS 2000; 14:1917-1920.
186. Kaul R, Plummer FA, Kimani J, Dons T, Kiama P, Rostron T, et al. HIV-1-specific mucosal CD8+ lymphocyte responses in the cervix of HIV-1-resistant prostitutes in Nairobi. J Immunol 2000; 164:1602-1611.
187. Barroso PF, Schechter M, Gupta P, Melo MF, Vieira M, Murta FC, et al. Effect of antiretroviral therapy on HIV shedding in semen. Ann Intern Med 2000; 133:280-284.
188. Nunnari G, Otero M, Dornadula G, Vanella M, Zhang H, Frank I, et al. Residual HIV-1 disease in seminal cells of HIV-1-infected men on suppressive HAART: latency without on-going cellular infections. AIDS 2002; 16:39-45.
189. Quinn TC, Wawer MJ, Sewankambo N, Serwadda D, Li C, Wabwire-Mangen F, et al. Viral load and heterosexual transmission of human immunodeficiency virus type 1. Rakai Project Study Group. N Engl J Med 2000; 342:921-929.
190. Taylor S, Pereira AS. Antiretroviral drug concentrations in semen of HIV-1 infected men. Sex Transm Infect 2001; 77:4-11.
191. Zhang H, Dornadula G, Beumont M, Livornese L, Jr, Van Uitert B, Henning K, et al. Human immunodeficiency virus type 1 in the semen of men receiving highly active antiretroviral therapy. N Engl J Med 1998; 339:1803-1809.
192. Dornadula G, Zhang H, VanUitert B, Stern J, Livornese L, Jr, Ingerman MJ, et al. Residual HIV-1 RNA in blood plasma of patients taking suppressive highly active antiretroviral therapy. JAMA 1999; 282:1627-1632.
193. Kroodsma KL, Kozal MJ, Hamed KA, Winters MA, Merigan TC. Detection of drug resistance mutations in the human immunodeficiency virus type 1 (HIV-1) pol gene: differences in semen and blood HIV-1 RNA and proviral DNA. J Infect Dis 1994; 170:1292-1295.
194. Eron JJ, Vernazza PL, Johnston DM, Seillier-Moiseiwitsch F, Alcorn TM, Fiscus SA, et al. Resistance of HIV-1 to antiretroviral agents in blood and seminal plasma: implications for transmission. AIDS 1998; 12:F181-189.
195. Eyre RC, Zheng G, Kiessling AA. Multiple drug resistance mutations in human immunodeficiency virus in semen but not blood of a man on antiretroviral therapy. Urology 2000; 55:591.
196. Boden D, Hurley A, Zhang L, Cao Y, Guo Y, Jones E, et al. HIV-1 drug resistance in newly infected individuals. JAMA 1999; 282:1135-1141.
197. Little SJ, Daar ES, D'Aquila RT, Keiser PH, Connick E, Whitcomb JM, et al. Reduced antiretroviral drug susceptibility among patients with primary HIV infection. JAMA 1999; 282:1142-1149.
198. Hecht FM, Grant RM, Petropoulos CJ, Dillon B, Chesney MA, Tian H, et al. Sexual transmission of an HIV-1 variant resistant to multiple reverse-transcriptase and protease inhibitors. N Engl J Med 1998; 339:307-311.
199. Chirgwin KD, Feldman J, Muneyyirci-Delale O, Landesman S, Minkoff H. Menstrual function in human immunodeficiency virus-infected women without acquired immunodeficiency syndrome. J Acquir Immune Defic Syndr Hum Retrovirol 1996; 12:489-494.
200. Cu-Uvin S, Wright DJ, Anderson D, Kovacs A, Watts DH, Cohn J, et al. Hormonal levels among HIV-1-seropositive women compared with high-risk HIV-seronegative women during the menstrual cycle. Women's Health Study (WHS) 001 and WHS 001a Study Team. J Womens Health Gend Based Med 2000; 9:857-863.
201. Mostad SB, Jackson S, Overbaugh J, Reilly M, Chohan B, Mandaliya K, et al. Cervical and vaginal shedding of human immunodeficiency virus type 1-infected cells throughout the menstrual cycle. J Infect Dis 1998; 178:983-991.
202. Greenblatt RM, Ameli N, Grant RM, Bacchetti P, Taylor RN. Impact of the ovulatory cycle on virologic and immunologic markers in HIV-infected women. J Infect Dis 2000; 181:82-90.
203. Reichelderfer P, Kovacs A, Wright DJ, Landay A, Cu-Unvin S, Burns DN, et al. The menstrual cycle does not affect human immunodeficiency virus type-1 levels in vaginal secretions. J Infect Dis 2002; 186:726-728.
204. Al-Harthi L, Wright DJ, Anderson D, Cohen M, Matity Ahu D, Cohn J, et al. The impact of the ovulatory cycle on cytokine production: evaluation of systemic, cervicovaginal, and salivary compartments. J Interferon Cytokine Res 2000; 20:719-724.
205. Al-Harthi L, Kovacs A, Coombs RW, Reichelderfer PS, Wright DJ, Cohen MH, et al. A menstrual cycle pattern for cytokine levels exists in HIV-positive women: implication for HIV vaginal and plasma shedding. AIDS 2001; 15:1535-1543.
206. Eckert LO, Hawes SE, Stevens CE, Koutsky LA, Eschenbach DA, Holmes KK. Vulvovaginal candidiasis: clinical manifestations, risk factors, management algorithm. Obstet Gynecol 1998; 92:757-765.
207. Homer PJ, Crowley T, Leece J, Hughes A, Smith GD, Caul EO. Chlamydia trachomatis detection and the menstrual cycle. Lancet 1998; 351:341-342.
208. Chow AW, Percival-Smith R, Bartlett KH, Goldring AM, Morrison BJ. Vaginal colonization with Escherichia coli in healthy women. Determination of relative risks by quantitative culture and multivariate statistical analysis. Am J Obstet Gynecol 1986; 154:120-126.
209. Thadepalli H, Savage EW, Jr., Salem FA, Roy I, Davidson EC, Jr. Cyclic changes in cervical microflora and their effect on infections following hysterectomy. Gynecol Obstet Invest 1982; 14:176-183.
210. Westrom L, Eschenbach D. Pelvic inflammatory disease. In: Holmes KK, Sparling PF, Mårdh P-A, Lemon SM, Stamm WE, Piot P, et al. editors. Sexually transmitted diseases, 3rd edn. New York: McGraw-Hill; 1999. Chapter 58, pp. 783-809.
211. Smith CB, Noble V, Bensch R, Ahlin PA, Jacobson JA, Latham RH. Bacterial flora of the vagina during the menstrual cycle: findings in users of tampons, napkins, and sea sponges. Ann Intern Med 1982; 96:948-951.
212. Olafsson K, Smith MS, Marshburn P, Carter SG, Haskill S. Variation of HIV infectibility of macrophages as a function of donor, stage of differentiation, and site of origin. J Acquir Immune Defic Syndr 1991; 4:154-164.
213. Yeaman GR, White HD, Howell A, Prabhala R, Wira CR. The mucosal immune system in the human female reproductive tract: potential insights into the heterosexual transmission of HIV. AIDS Res Hum Retrovir 1998; 14 (Suppl 1):S57-62.
214. Fahey JV, Humphrey SL, Stern JE, Wira CR. Secretory component production by polarized epithelial cells from the human female reproductive tract. Immunol Invest 1998; 27:167-180.
215. Kutteh WH, Prince SJ, Hammond KR, Kutteh CC, Mestecky J. Variations in immunoglobulins and IgA subclasses of human uterine cervical secretions around the time of ovulation. Clin Exp Immunol 1996; 104:538-542.
216. Lu FX, Ma Z, Rourke T, Srinivasan S, McChesney M, Miller CJ. Immunoglobulin concentrations and antigen-specific antibody levels in cervicovaginal lavages of rhesus macaques are influenced by the stage of the menstrual cycle. Infect Immun 1999; 67:6321-6328.
217. Fichorova RN, Anderson DJ. Differential expression of immunobiological mediators by immortalized human cervical and vaginal epithelial cells. Biol Reprod 1999; 60:508-514.
218. Butera ST, Pisell TL, Limpakarnjanarat K, Young NL, Hodge TW, Mastro TD, et al. Production of a novel viral suppressive activity associated with resistance to infection among female sex workers exposed to HIV type 1. AIDS Res Hum Retrovir 2001; 17:735-744.
219. Belec L, Ghys PD, Hocini H, Nkengasong JN, Tranchot-Diallo J, Diallo MO, et al. Cervicovaginal secretory antibodies to human immunodeficiency virus type 1 (HIV-1) that block viral transcytosis through tight epithelial barriers in highly exposed HIV-1-seronegative African women. J Infect Dis 2001; 184:1412-1422.
220. Biasin M, Caputo SL, Speciale L, Colombo F, Racioppi L, Zagliani A, et al. Mucosal and systemic immune activation is present in human immunodeficiency virus-exposed seronegative women. J Infect Dis 2000; 182:1365-1374.
221. Hillier SL. The vaginal microbial ecosystem and resistance to HIV. AIDS Res Hum Retrovir 1998; 14 (Suppl 1):S17-21.
222. Zasloff M. Antimicrobial peptides of multicellular organisms. Nature 2002; 415:389-395.
223. Levy O. Antimicrobial proteins and peptides of blood: templates for novel antimicrobial agents. Blood 2000; 96:2664-2672.
224. Shugars DC, Wahl SM. The role of the oral environment in HIV-1 transmission. J Am Dent Assoc 1998; 129:851-858.
225. Davidson JB, Douglas GC. Modulation of integrin function inhibits HIV transmission to epithelial cells and fertilization. J Reprod Immunol 1998; 41:271-290.
226. Nakashima H, Yamamoto N, Masuda M, Fujii N. Defensins inhibit HIV replication in vitro. AIDS 1993; 7:1129.
227. Tam JP, Wu C, Yang JL. Membranolytic selectivity of cystine-stabilized cyclic protegrins. Eur J Biochem 2000; 267:3289-3300.
228. Sallenave JM, Si-Ta har M, Cox G, Chignard M, Gauldie J. Secretory leukocyte proteinase inhibitor is a major leukocyte elastase inhibitor in human neutrophils. J Leukoc Biol 1997; 61:695-702.
229. Hocini H, Becquart P, Bouhlal H, Adle-Biassette H, Kazatchkine MD, Belec L. Secretory leukocyte protease inhibitor inhibits infection of monocytes and lymphocytes with human immunodeficiency virus type 1 but does not interfere with transcytosis of cell-associated virus across tight epithelial barriers. Clin Diagn Lab Immunol 2000; 7:515-518.
230. Quayle AJ, Porter EM, Nussbaum AA, Wang YM, Brabec C, Yip KP, et al. Gene expression, immunolocalization, and secretion of human defensin-5 in human female reproductive tract. Am J Pathol 1998; 152:1247-1258.
231. Valore EV, Park CH, Quayle AJ, Wiles KR, McCray PB, Jr., Ganz T. Human beta-defensin-1: an antimicrobial peptide of urogenital tissues. J Clin Invest 1998; 101:1633-1642.
232. Hill JA, Anderson DJ. Human vaginal leukocytes and the effects of vaginal fluid on lymphocyte and macrophage defense functions. Am J Obstet Gynecol 1992; 166:720-726.
233. Klebanoff SJ, Hillier SL, Eschenbach DA, Waltersdorph AM. Control of the microbial flora of the vagina by H202-generatins lactobacilli. J Infect Dis 1991; 164:94-100.
234. Draper DL, Landers DV, Krohn MA, Hillier SL, Wiesenfeld HC, Heine RP. Levels of vaginal secretory leukocyte protease inhibitor are decreased in women with lower reproductive tract infections. Am J Obstet Gynecol 2000; 183:1243-1248.
235. Fahey JV, Wira CR. Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture. J Infect Dis 2002; 185:1606-1613.
236. Marx PA, Spira AI, Gettie A, Dailey PJ, Veazey RS, Lackner AA, et al. Progesterone implants enhance SIV vaginal transmission and early virus load. Nat Med 1996; 2:1084-1089.
237. Mauck CK, Callahan MM, Baker J, Arbogast K, Veazey R, Stock R, et al. The effect of one injection of Depo-Provera on the human vaginal epithelium and cervical ectopy. Contraception 1999; 60:15-24.
238. Lunardi-Iskandar Y, Bryant JL, Blattner WA, Hung CL, Flamand L, Gill P, et al. Effects of a urinary factor from women in early pregnancy on HIV-1, SIV and associated disease. Nat Med 1998; 4:428-434.
239. Lee-Huang S, Huang PL, Sun Y, Kung HF, Blithe DL, Chen HC. Lysozyme and RNases as anti-HIV components in beta-core preparations of human chorionic gonadotropin. Proc Natl Acad Sci U S A 1999; 96:2678-2681.
240. Gruber A, Lukasser-Vogl E, Borg-von Zepelin M, Dierich MP, Wurzner R. Human immunodeficiency virus type 1 gp160 and gp41 binding to Candida albicans selectively enhances candidal virulence in vitro. J Infect Dis 1998; 177:1057-1063.
241. Wurzner R, Gruber A, Stoiber H, Spruth M, Chen YH, Lukasser-Vogl E, et al. Human immunodeficiency virus type 1 gp41 binds to Candida albicans via complement C3-like regions. J Infect Dis 1997; 176:492-498.
242. Fiore JR, La Grasta L, Di Stefano M, Buccoliero G, Pastore G, Angarano G. The use of serum-free medium delays, but does not prevent, the cytotoxic effects of seminal plasma in lymphocyte cultures: implications for studies on HIV infection. New Microbiol 1997; 20:339-344.
243. Puddu P, Borghi P, Gessani S, Valenti P, Belardelli F, Seganti L. Antiviral effect of bovine lactoferrin saturated with metal ions on early steps of human immunodeficiency virus type 1 infection. Int J Biochem Cell Biol 1998; 30:1055-1062.
244. Klebanoff SJ, Kazazi F. Inactivation of human immunodeficiency virus type 1 by the amine oxidase-peroxidase system. J Clin Microbiol 1995; 33:2054-2057.
245. Ohlsson K, Bjartell A, Lilja H. Secretory leucocyte protease inhibitor in the male genital tract: PSA- induced proteolytic processing in human semen and tissue localization. J Androl 1995; 16:64-74.
246. Shugars DC. Endogenous mucosal antiviral factors of the oral cavity. J Infect Dis 1999; 179 (Suppl 3):S431-435.
247. Autiero M, Gaubin M, Mani JC, Castejon C, Martin M, el Marhomy S, et al. Surface plasmon resonance analysis of gp17, a natural CD4 ligand from human seminal plasma inhibiting human immunodeficiency virus type-1 gp120-mediated syncytium formation. Eur J Biochem 1997; 245:208-213.
248. Zhang H, Dornadula G, Pomerantz RJ. Endogenous reverse transcription of human immunodeficiency virus type 1 in physiological microenvironments: an important stage for viral infection of nondividing cells. J Virol 1996; 70:2809-2824.
249. Kelly RW, Critchley HO. Immunomodulation by human seminal plasma: a benefit for spermatozoon and pathogen? Hum Reprod 1997; 12:2200-2207.
250. Kelly RW. Prostaglandins in primate semen: biasing the immune system to benefit spermatozoa and virus? Prostaglandins Leukot Essent Fatty Acids 1997; 57:113-118.
251. Kelly RW, Carr GG, Critchley HO. A cytokine switch induced by human seminal plasma: an immune modulation with implications for sexually transmitted disease. Hum Reprod 1997; 12:677-681.
252. Thivierge M, Le Gouill C, Tremblay MJ, Stankova J, Rola-Pleszczynski M. Prostaglandin E2 induces resistance to human immunodeficiency virus-1 infection in monocyte-derived macrophages: downregulation of CCR5 expression by cyclic adenosine monophosphate. Blood 1998; 92:40-45.
253. Poss M, Rodrigo AG, Gosink JJ, Learn GH, de Vange Panteleeff D, Martin HL, Jr, et al. Evolution of envelope sequences from the genital tract and peripheral blood of women infected with clade A human immunodeficiency virus type 1. J Virol 1998; 72:8240-8251.
254. Overbaugh J, Kreiss J, Poss M, Lewis P, Mostad S, John G, et al. Studies of human immunodeficiency virus type 1 mucosal viral shedding and transmission in Kenya. J Infect Dis 1999; 179 (Suppl 3):S401-404.
255. Hladik F, Lentz G, Delpit E, McElroy A, McElrath MJ. Coexpression of CCR5 and IL-2 in human genital but not blood T cells: implications for the ontogeny of the CCR5+ Th1 phenotype. J Immunol 1999; 163:2306-2313.
256. Hladik F, Lentz G, Akridge RE, Peterson G, Kelley H, McElroy A, et al. Dendritic cell-T-cell interactions support coreceptor-independent human immunodeficiency virus type 1 transmission in the human genital tract. J Virol 1999; 73:5833-5842.
257. Vernazza PL, Eron JJ, Cohen MS, van der Horst CM, Troiani L, Fiscus SA. Detection and biologic characterization of infectious HIV-1 in semen of seropositive men. AIDS 1994; 8:1325-1329.
258. Delwart EL, Mullins JI, Gupta P, Learn GH, Jr, Holodniy M, Katzenstein D, et al. Human immunodeficiency virus type 1 populations in blood and semen. J Virol 1998; 72:617-623.
259. Iversen AK, Learn GH, Fugger L, Gerstoft J, Mullins JI, Skinhoj P. Presence of multiple HIV subtypes and a high frequency of subtype chimeric viruses in heterosexually infected women. J Acquir Immune Defic Syndr 1999; 22:325-332.
260. Byrn RA, Kiessling AA. Analysis of human immunodeficiency virus in semen: indications of a genetically distinct virus reservoir. J Reprod Immunol 1998; 41:161-176.
261. Alaeus A. Significance of HIV-1 genetic subtypes. Scand J Infect Dis 2000; 32:455-463.
262. Blackard JT, Cohen DE, Mayer KH. Human immunodeficiency virus superinfection and recombination: current state of knowledge and potential clinical consequences. Clin Infect Dis 2002; 34:1108-1114.
263. Sutthent R, Sumrangsurp K, Wirachsilp P, Chaisilwattana P, Roongpisuthipong A, Chaiyakul P, et al. Diversity of HIV-1 subtype E in semen and cervicovaginal secretion. J Hum Virol 2001; 4:260-268.
264. Girard M, Mahoney J, Wei Q, van der Ryst E, Muchmore E, Barre-Sinoussi F, et al. Genital infection of female chimpanzees with human immunodeficiency virus type 1. AIDS Res Hum Retrovir 1998; 14:1357-1367.
265. Poss M, Overbaugh J. Variants from the diverse virus population identified at seroconversion of a clade A human immunodeficiency virus type 1-infected woman have distinct biological properties. J Virol 1999; 73:5255-5264.
266. Mastro TD, Satten GA, Nopkesom T, Sangkharomya S, Longini IM, Jr. Probability of female-to-male transmission of HIV-1 in Thailand. Lancet 1994; 343:204-207.
267. European Study Group on Heterosexual Transmission of HIV. Comparison of female to male and male to female transmission of HIV in 563 stable couples. BMJ 1992; 304:809-813.
268. Sterling TR, Lyles CM, Vlahov D, Astemborski J, Margolick JB, Quinn TC. Sex differences in longitudinal human immunodeficiency virus type 1 RNA levels among seroconverters. J Infect Dis 1999; 180:666-672.
269. Fideli US, Allen SA, Musonda R, Trask S, Hahn BH, Weiss H, et al. Virologic and immunologic determinants of heterosexual transmission of human immunodeficiency virus type 1 in Africa. AIDS Res Hum Retrovir 2001; 17:901-910.
270. Gray RH, Wawer MJ, Brookmeyer R, Sewankambo NK, Serwadda D, Wabwire-Mangen F, et al. Probability of HIV-1 transmission per coital act in monogamous, heterosexual, HIV-1-discordant couples in Rakai, Uganda. Lancet 2001; 357:1149-1153.
271. Fiore JR, Zhang YJ, Bjorndal A, Di Stefano M, Angarano G, Pastore G, et al. Biological correlates of HIV-1 heterosexual transmission. AIDS 1997; 11:1089-1094.
272. Collins KB, Patterson BK, Naus GJ, Landers DV, Gupta P. Development of an in vitro organ culture model to study transmission of HIV-1 in the female genital tract. Nat Med 2000; 6:475-479.
273. Greenhead P, Hayes P, Watts PS, Laing KG, Griffin GE, Shattock RJ. Parameters of human immunodeficiency virus infection of human cervical tissue and inhibition by vaginal virucides. J Virol 2000; 74:5577-5586.
274. Shattock RJ, Griffin GE, Gorodeski GI. In vitro models of mucosal HIV transmission. Nat Med 2000; 6:607-608.
275. Moench TR, Chipato T, Padian NS. Preventing disease by protecting the cervix: the unexplored promise of internal vaginal barrier devices. AIDS 2001; 15:1595-1602.
276. Dezzutti CS, Guenthner PC, Cummins JE, Jr, Cabrera T, Marshall JH, Dillberger A, et al. Cervical and prostate primary epithelial cells are not productively infected but sequester human immunodeficiency virus type 1. J Infect Dis 2001; 183:1204-1213.
277. Hu J, Gardner MB, Miller CJ. Simian immunodeficiency virus rapidly penetrates the cervicovaginal mucosa after intravaginal inoculation and infects intraepithelial dendritic cells. J Virol 2000; 74:6087-6095.
278. Royce RA, Sena A, Cates W, Jr, Cohen MS. Sexual transmission of HIV. N Engl J Med 1997; 336:1072-1078.
279. O'Farrell N, Egger M. Circumcision in men and the prevention of HIV infection: a 'meta- analysis' revisited. Int J STD AIDS 2000; 11:137-142.
280. Kelly R, Kiwanuka N, Wawer MJ, Serwadda D, Sewankambo NK, Wabwire-Mangen F, et al. Age of male circumcision and risk of prevalent HIV infection in rural Uganda. AIDS 1999; 13:399-405.
281. Gray RH, Kiwanuka N, Quinn TC, Sewankambo NK, Serwadda D, Mangen FW, et al. Male circumcision and HIV acquisition and transmission: cohort studies in Rakai, Uganda. Rakai Project Team. AIDS 2000; 14:2371-2381.
282. Quinn TC, Wawer MJ, Sewankambo NK. A study in rural Uganda of heterosexual transmission of human immunodeficiency virus. N Engl J Med 2000; 343:365; discussion 364-365.
283. Padian NS, Shiboski SC, Glass SO, Vittinghoff E. Heterosexual transmission of human immunodeficiency virus (HIV) in northern California: results from a ten-year study. Am J Epidemiol 1997; 146:350-357.
284. Wright TC, Ferenczy A. Chapter 6: Benign diseases of the cervix. In: Kurman RJ, editor. Blaustein's pathology of the female genital tract, 5th edn. New York: Springer-Verlag; 2002.
285. Muller CH, Coombs RW, Krieger JN. Effects of clinical stage and immunological status on semen analysis results in human immunodeficiency virus type 1-seropositive men. Andrologia 1998; 30:15-22.
286. Quayle AJ, Xu C, Tucker L, Anderson DJ. The case against an association between HIV-1 and sperm: molecular evidence. J Reprod Immunol 1998; 41:127-136.
287. Cu-Uvin S, Hogan JW, Caliendo AM, Harwell J, Mayer KH, Carpenter CC. Association between bacterial vaginosis and expression of human immunodeficiency virus type 1 RNA in the female genital tract. Clin Infect Dis 2001; 33:894-896.